Abstract:
:In order to confirm the presence of a phosphoenolpyruvate (PEP)-dependent sugar-phosphotransferase system (PTS) in the mollicute Acholeplasma florum we studied the ability of cell free extracts of this organism to phosphorylate glucose and/or fructose in the presence of PEP. We also cloned and sequenced a DNA fragment coding for a putative polypeptide showing significant similarity with the enzyme II of the beta-glucoside PTS of Escherichia coli. Taken together, these results show that A florum possesses a fructose-PTS, but not a glucose-PTS, and that the amino acid sequence deduced from the DNA fragment is related to beta-glucoside and sucrose enzymes II of PTS from various bacteria.
journal_name
Biochimiejournal_title
Biochimieauthors
Navas-Castillo J,Laigret F,Hocquellet A,Chang CJ,Bove JMdoi
10.1016/0300-9084(93)90098-dsubject
Has Abstractpub_date
1993-01-01 00:00:00pages
675-9issue
8eissn
0300-9084issn
1638-6183pii
0300-9084(93)90098-Djournal_volume
75pub_type
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