Characterization of the lipid envelope of exosome encapsulated HEV particles protected from the immune response.


:The hepatitis E virus (HEV) is the most common cause of acute hepatitis worldwide. Although HEV is a small, naked RNA virus, HEV particles become associated with lipids in the blood of infected patients and in the supernatant of culture systems. The egress of these particles from cells implies the exocytosis pathway but the question of the role of the resulting HEV RNA containing exosomes and the nature of the lipids they contain has not been fully addressed. We determined the lipid proportions of exosomes from uninfected and HEV-infected cells and their role in HEV spreading. We cultured a suitable HEV strain on HepG2/C3A cells and analyzed the population of exosomes containing HEV RNA using lipidomics methods and electron microscopy. We also quantified HEV infectivity using an infectivity endpoint method based on HEV RNA quantification to calculate the tissue culture infectious dose 50. Exosomes produced by HEV-infected HepG2/C3A cells contained encapsidated HEV RNA. These HEV RNA-containing exosomes were infectious but ten times less than stools. HEV from stools, but not exosome-associated HEV from culture supernatant, was neutralized by anti-HEV antibodies in a dose-dependent manner. HEV infection did not influence the morphology or lipid proportions of the bulk of exosomes. These exosomes contained significantly more cholesterol, phosphatidylserine, sphingomyelin and ceramides than the parent cells, but less phosphoinositides and polyunsaturated fatty acids. Exosomes play a major role in HEV egress but HEV infection does not modify the characteristics of the bulk of exosomes produced by infected cells. PS and cholesterol enriched in these vesicles could then be critical for HEV entry. HEV particles in exosomes are protected from the immune response which could lead to the wide circulation of HEV in its host.






Chapuy-Regaud S,Dubois M,Plisson-Chastang C,Bonnefois T,Lhomme S,Bertrand-Michel J,You B,Simoneau S,Gleizes PE,Flan B,Abravanel F,Izopet J




Has Abstract


2017-10-01 00:00:00












  • The roles of nucleolin subcellular localization in cancer.

    abstract::Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...


    pub_type: 杂志文章,评审


    authors: Berger CM,Gaume X,Bouvet P

    更新日期:2015-06-01 00:00:00

  • New nomenclature of the non-rod-shaped lactic acid bacteria.

    abstract::Bacteria of the Lactococcus (formerly lactic Streptococcus) genus are described to emphasize certain less well-known facts of importance in their successful use as dairy starters. Other streptococci (non-rod forms) are briefly characterized to emphasize their usefulness in industrial milk fermentations. ...


    pub_type: 杂志文章


    authors: Sandine WE

    更新日期:1988-04-01 00:00:00

  • Characterization of HU-like protein from Bifidobacterium longum.

    abstract::A HU-like protein (HBl) of Bifidobacterium longum was purified and characterized. HBl is heat-stable and acid-resistant, and has a molecular weight of about 9.1 kDa as estimated by its mobility on electrophoresis. HBl is intermediate in basicity (pI 9.8) between the HU-1 and HU-2 proteins of Escherichia coli, and is d...


    pub_type: 杂志文章


    authors: Goshima N,Kano Y,Imamoto F

    更新日期:1990-04-01 00:00:00

  • Characterization and purification of messenger RNAs containing poly A in insect imaginal disks.

    abstract::The presence of a fragment of polyA resistant to both T1 and p ribonucleases in mRNAs extracted from wing imaginal disks of an insect, Pieris brassicae, is reported. Its length was approximatively 150 nucleotides. PolyU sepharose affinity chromatography was subsequently used for purification of these polyA(+)mRNA mole...


    pub_type: 杂志文章


    authors: Tarroux P

    更新日期:1975-01-01 00:00:00

  • Alteration of plasma phospholipid fatty acid profile in patients with septic shock.

    abstract::In septic shock patients, alterations of plasma phospholipid fatty acid profile have never been described. The purpose of this monocentric, non-interventional, observational prospective study was to describe this fatty acid profile in the early phase of septic shock in intensive care unit. Thirty-seven adult patients ...


    pub_type: 杂志文章


    authors: Rival T,Cinq-Frais C,Silva-Sifontes S,Garcia J,Riu B,Salvayre R,Genestal M,Caspar-Bauguil S

    更新日期:2013-11-01 00:00:00

  • Isotopic discrimination between D-[1-(13)C]fructose and D-[2-(13)C]fructose in rat liver cells.

    abstract::When liver cells from either normal or hereditarily diabetic rats are exposed to (13)C-enriched D-fructose (10 mM) and unlabelled D-glucose (also 10 mM) in the presence of D(2)O, the output of (13)C-enriched D-glucose generated from D-[1-(13)C]fructose is significantly lower than that from D-[2-(13)C]fructose. This co...


    pub_type: 杂志文章


    authors: Malaisse WJ,Willem R

    更新日期:2004-02-01 00:00:00

  • Structural and functional consequences of subunit interactions in glyceraldehyde 3-phosphate dehydrogenase.

    abstract::A variety of species of GPDH undergo acylation at two of the four active cystein sites per mole of tetrameric enzyme. This reaction requires tightly bound NAD+, a situation restricted to two of the four NAD sites per tetramer. S leads to N acyl transfer from cysteins to lysine in the diacyl enzyme yields an inactive e...


    pub_type: 杂志文章


    authors: Malhotra OP,Bernhard SA,Seydoux F

    更新日期:1981-02-01 00:00:00

  • Phage resistance in lactic acid bacteria.

    abstract::The interactions between lactic acid bacteria and their phages are commercially significant. Current research has focused on the elucidation of the mechanisms and genetics of phage resistance. Phage resistance genes have been linked to plasmid DNA for Streptococcus lactis and Streptococcus cremoris, and preliminary st...


    pub_type: 杂志文章,评审


    authors: Sanders ME

    更新日期:1988-03-01 00:00:00

  • [Heterogeneity of ciguatoxins extracted from fish caught in the French Antilles].

    abstract::Ciguatoxin-like substances were extracted from the viscera or the flesh of eight Caribbean fish species, including small invertebrate feeders and large carnivores. The had similar properties, i.e. pharmacological action, solubility, chromatographic behaviour on silicic acid or Sephadex LH 20 column, stability in a wea...


    pub_type: 杂志文章


    authors: Vernoux JP,Abbad el Andaloussi S

    更新日期:1986-03-01 00:00:00

  • Serotonin in retina.

    abstract::The expression of serotonin (5-HT) in the retina was first reported in the sixties. The detection of vesicular monoamine transporter and serotonin receptors in several retinal cells confirm that 5-HT is playing a neuromodulatory role in this structure. Whereas signaling pathways activated by 5-HT receptor binding has ...


    pub_type: 杂志文章,评审


    authors: Masson J

    更新日期:2019-06-01 00:00:00

  • Properties of chromosomal proteins of human leukemic cells.

    abstract::Purified chromatin isolated from lymphocytic cells derived from patients with acute leukemia, or other lymphoproliferative disorders has been compared with chromatin isolated from normal human lymphocytic cells by gel electrophoresis and differential gradient ultracentrifugation. Thermal denaturation studies showed hi...


    pub_type: 杂志文章


    authors: Desai LS,Wulff UC,Foley GE

    更新日期:1975-01-01 00:00:00

  • [Study, by an enzymatic method, of the isomers of L-proline (3H-4) and of the effects of preservation].

    abstract::By use of procollagen proline hydroxylas which catalyses the substitution of the hydrogen in the trans position on carbon 4 with an hydroxyl on L-proline residues of procollagen, we have shown that the L-[3H-4] proline product of C.E.N. Saclay contained 29 per cent of the trans and 71 per cent of the cis form. It was ...


    pub_type: 杂志文章


    authors: Farjanel J,Pichat L,Audinot M,Perier C,Frey J

    更新日期:1976-01-01 00:00:00

  • Characterization of conserved microRNAs from five different cucurbit species using computational and experimental analysis.

    abstract::MicroRNAs (miRNAs) are ∼21 nt non-coding small RNAs which regulate gene expression at the post-transcriptional level in plants and animals. Until recently, only limited numbers of miRNAs were identified in Cucurbitaceae, a large flowering plant family. In this study, 220 potential miRNA candidates were identified from...


    pub_type: 杂志文章


    authors: Hu J,Sun L,Zhu Z,Zheng Y,Xiong W,Ding Y

    更新日期:2014-07-01 00:00:00

  • Stereospecific hydrogenations with immobilized microbial cells or enzymes.

    abstract::Stereospecific hydrogenations according to the general scheme [formula: see text] are of interest from a preparative and mechanistic point of view. Proteus mirabilis is suitable for the hydrogenation of a-keto-acids to R-hydroxy-acids, and a Clostridium strain for the reduction of enoates. Both have been immobilized i...


    pub_type: 杂志文章


    authors: Tischer W,Tiemeyer W,Simon H

    更新日期:1980-01-01 00:00:00

  • PssO, a unique extracellular protein important for exopolysaccharide synthesis in Rhizobium leguminosarum bv. trifolii.

    abstract::Synthesis and secretion of polysaccharides by Gram-negative bacteria are a result of a concerted action of enzymatic and channel-forming proteins localized in different compartments of the cell. The presented work comprises functional characterization of PssO protein encoded within the previously identified, chromosom...


    pub_type: 杂志文章


    authors: Marczak M,Mazur A,Gruszecki WI,Skorupska A

    更新日期:2008-11-01 00:00:00

  • Lactic acid bacteria: model systems for in vivo studies of sugar transport and metabolism in gram-positive organisms.

    abstract::Lactic acid bacteria provide a model system for the in vivo study of mechanisms pertaining to the regulation of sugar transport and metabolism by microorganisms. Recent studies with resting and growing cells of the homofermentative Streptococci and Lactobacilli have yielded evidence for hitherto unsuspected regulatory...


    pub_type: 杂志文章,评审


    authors: Thompson J

    更新日期:1988-03-01 00:00:00

  • Interaction of Fis protein with DNA: bending and specificity of binding.

    abstract::The Escherichia coli Fis protein is a dimeric DNA-binding protein whose specific binding sites share a weak consensus sequence. Use of the gel retardation technique indicates that binding of Fis on a linear DNA fragment leads to the formation of a ladder of defined retarded complexes, independently of the presence of ...


    pub_type: 杂志文章


    authors: Bétermier M,Galas DJ,Chandler M

    更新日期:1994-01-01 00:00:00

  • Mapping the eosinophil cationic protein antimicrobial activity by chemical and enzymatic cleavage.

    abstract::The eosinophil cationic protein (ECP) is a human antimicrobial protein involved in the host immune defense that belongs to the pancreatic RNase A family. ECP displays a wide range of antipathogen activities. The protein is highly cationic and its bactericidal activity is dependant on both cationic and hydrophobic surf...


    pub_type: 杂志文章


    authors: Sánchez D,Moussaoui M,Carreras E,Torrent M,Nogués V,Boix E

    更新日期:2011-02-01 00:00:00

  • Amino acid sequences of cytochromes c2 and c' from the moderately halophilic purple phototrophic bacterium Rhodospirillum salexigens.

    abstract::Rhodospirillum salexigens is a moderately halophilic purple phototrophic bacterium which grows optimally in 8% NaCl. The amino acid sequences of the two principal soluble cytochromes c have been determined. One of these is a cytochrome c2, similar in size to mitochondrial cytochrome c. While clearly of the same sequen...


    pub_type: 杂志文章


    authors: Ambler RP,Daniel M,Meyer TE,Kamen MD

    更新日期:1994-01-01 00:00:00

  • Complete purification and studies on the structural and kinetic properties of two forms of yeast valyl-tRNA synthetase.

    abstract::Two forms of baker's yease valyl-tRNA synthetase have been purified to apparent homogeneity by classical methods. It was demonstrated that one of the two forms of the enzyme originates from the other by proteolysis, the respective amounts of each form depending on the physiological state of the yeast. The species main...


    pub_type: 杂志文章


    authors: Kern D,Giegé R,Robre-Saul S,Boulanger Y,Ebel JP

    更新日期:1975-01-01 00:00:00

  • Comparison of histidine recognition in human and trypanosomatid histidyl-tRNA synthetases.

    abstract::As part of a project aimed at obtaining selective inhibitors and drug-like compounds targeting tRNA synthetases from trypanosomatids, we have elucidated the crystal structure of human cytosolic histidyl-tRNA synthetase (Hs-cHisRS) in complex with histidine in order to be able to compare human and parasite enzymes. The...


    pub_type: 杂志文章


    authors: Koh CY,Wetzel AB,de van der Schueren WJ,Hol WG

    更新日期:2014-11-01 00:00:00

  • Dyskeratosis congenita: the diverse clinical presentation of mutations in the telomerase complex.

    abstract::Dyskeratosis congenita is an inherited syndrome characterised by mucocutaneous features, bone marrow failure, an increased risk of malignancy and other somatic abnormalities. There is a considerable range of clinical severity and in its occult form the disease may present as idiopathic aplastic anaemia. Genes responsi...


    pub_type: 杂志文章,评审


    authors: Vulliamy TJ,Dokal I

    更新日期:2008-01-01 00:00:00

  • IL-28 and IL-29: newcomers to the interferon family.

    abstract::IL-28 and IL-29 were recently described as members of a new cytokine family that shares with type I interferon (IFN) the same Jak/Stat signalling pathway driving expression of a common set of genes. Accordingly, they have been named IFN lambda. IFNs lambda exhibit several common features with type I IFNs: antiviral ac...


    pub_type: 杂志文章,评审


    authors: Uzé G,Monneron D

    更新日期:2007-06-01 00:00:00

  • MicroRNA-149 is associated with clinical outcome in human neuroblastoma and modulates cancer cell proliferation through Rap1 independent of MYCN amplification.

    abstract:PURPOSE:We evaluated the clinical relevance of microRNA-149 (miR-149) in neuroblastoma (NB) and its functional roles in regulating NB proliferation in vitro. METHODS:QRT-PCR was used to evaluate miR-149 expression in NB cell lines and primary NB tumors. Association between endogenous miR-149 expression in primary NB t...


    pub_type: 杂志文章


    authors: Xu Y,Chen X,Lin L,Chen H,Yu S,Li D

    更新日期:2017-08-01 00:00:00

  • The structure of the gene for ribosomal protein L5 in the archaebacterium Sulfolobus acidocaldarius.

    abstract::The gene for the ribosomal protein L5 from the archaebacterium Sulfolobus acidocaldarius has been isolated and sequenced. The gene codes for a basic protein of molecular weight 29 165 Da. This protein shows substantial similarity to the equivalent protein from other archaebacteria as well as from yeast, and considerab...


    pub_type: 杂志文章


    authors: Yang D,Günther I,Matheson AT,Auer J,Spicker G,Böck A

    更新日期:1991-06-01 00:00:00

  • DNA binding thermodynamics and site stoichiometry as a function of polyamide size.

    abstract::The interactions of 6-8 ring hairpin polyamides (PAs) with the minor groove of DNA have been investigated extensively. More recent studies of large antiviral PAs (14-20 rings) active against small DNA tumor viruses lead to questions regarding the extent to which the DNA binding behaviors of the well studied, smaller P...


    pub_type: 杂志文章


    authors: Song Y,Niederschulte J,Bales KN,Park AH,Bashkin JK,Dupureur CM

    更新日期:2019-10-01 00:00:00

  • GKN1 expression in gastric cancer cells is negatively regulated by miR-544a.

    abstract::Gastrokine1 (GKN1), important for maintaining the physiological function of the gastric mucosa, is highly expressed in the stomach of healthy individuals but is down-regulated or absent in gastric tumor tissues and derived cell lines. The mechanisms underlying GKN1 gene inactivation are still unknown. We previously sh...


    pub_type: 杂志文章


    authors: Stella di Stadio C,Faraonio R,Federico A,Altieri F,Rippa E,Arcari P

    更新日期:2019-12-01 00:00:00

  • [A new protein from human plasma lipoproteins: isolation and partial characterization of apolipoprotein G].

    abstract::A new apolipoprotein has been identified in VHDL1 and in HDL. This protein is immunologically distinct from already isolated apoproteins. It was isolated by column chromatography on hydroxylapatite. In polyacrylamide gel electrophoresis, its mobility is very close to that of apo D. The amino acid composition differs f...


    pub_type: 杂志文章


    authors: Ayrault-Jarrier M,Alix JF,Polonovski J

    更新日期:1978-01-01 00:00:00

  • The increased accumulation of structurally modified versican and decorin is related with the progression of laryngeal cancer.

    abstract::Versican and decorin, two proteoglycans (PGs) with contradictory roles in the pathophysiology of cancer, comprise important stromal components in many tumor types and play a crucial role in the progression of cancer. In this study, we provide direct evidence for a significant and stage-related accumulation of versican...


    pub_type: 杂志文章


    authors: Skandalis SS,Theocharis AD,Papageorgakopoulou N,Vynios DH,Theocharis DA

    更新日期:2006-09-01 00:00:00

  • A method for deciding whether two experimental fluorescence anisotropy decay curves are significantly different.

    abstract::We describe a method to compare two fluorescence anisotropy decay curves. After numerical deconvolution of both decays by a non a priori method [1], their difference, D, is considered. The variance is computed for each point of D. A confidence interval is defined which allows a decision to be made as to the significan...


    pub_type: 杂志文章


    authors: Genest D,Privat JP

    更新日期:1984-09-01 00:00:00