Abstract:
:In order to identify conserved potential secondary structures within translational start sites, mRNA sequences derived from different species were studied with programs able to depict such features. The potential secondary structure of 71 bases around the initiator AUG or AUGs in the coding sequences of 290 eukaryotic mRNAs was first examined and compared to 290 similarly analyzed regions derived from prokaryotic mRNA sequences (Nucleic Acids Res (1987) 15, 345-360). In both sets of sequences the initiator codon was often found to be in an open potential structure whereas a denser region characterized by nearly-periodic spacings defined the coding regions. Randomization of the sequences obliterated the observed patterns suggesting that the structure of the mRNA may determine these differences. Three sets of eukaryotic and prokaryotic mRNAs of approximately equal length were analyzed and found to preserve an open unpaired non-coding region 5' to the start codon. The start codon was found free of potential secondary structure in over 80% of all the sequences analyzed. These data, and study of mutants that restrict the accessibility of the start codon to the ribosomal initiation complex, suggest that both the prokaryotic and eukaryotic mRNA start sites must occur free of potential secondary structure for efficient initiation. A striking difference of the eukaryotic mRNA sequences analyzed was the high propensity of the coding region vicinal to the start codon to form secondary structures. Certain translation-defective mutants exhibit impaired formation of these secondary structures suggesting that the structure of the coding regions adjacent to the start codons of eukaryotic mRNAs may be an important, thus far unexamined, determinant of initiation. We propose that, for all genes studied, the transition in secondary structure between the coding and non-coding regions may be an important determinant of initiation.
journal_name
Biochimiejournal_title
Biochimieauthors
Ganoza MC,Louis BGdoi
10.1016/0300-9084(94)90120-1subject
Has Abstractpub_date
1994-01-01 00:00:00pages
428-39issue
5eissn
0300-9084issn
1638-6183pii
0300-9084(94)90120-1journal_volume
76pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Epigenetic alterations are involved in every step of carcinogenesis. The development of chromatin-modifying agents (CMAs) has provided the ability to fight cancer by reversing these alterations. Currently, four CMAs have been approved for cancer treatment; two DNA demethylating agents and two deacetylase inhibitors. A...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.05.012
更新日期:2012-11-01 00:00:00
abstract::The mechanism of action on rabbit red cells of Treponema hyodysenteriae hemolysin was studied using volume analysis and release of hemoglobin. While fixation of the hemolysin on the erythrocytes is temperature independent, it appears that hemolysis is temperature dependent. The kinetics of hemolysis proceed according ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80134-9
更新日期:1980-01-01 00:00:00
abstract::Diacylglycerol kinase (DGK) is a lipid-metabolizing enzyme that phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). DGKδ is highly expressed in the skeletal muscle, and a decrease in DGKδ expression increases the severity of type 2 diabetes. However, the role of DGKδ in myogenic differentiation is st...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.05.017
更新日期:2018-08-01 00:00:00
abstract::The interactions between lactic acid bacteria and their phages are commercially significant. Current research has focused on the elucidation of the mechanisms and genetics of phage resistance. Phage resistance genes have been linked to plasmid DNA for Streptococcus lactis and Streptococcus cremoris, and preliminary st...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(88)90215-5
更新日期:1988-03-01 00:00:00
abstract::Fluorometry using a substrate DNA labeled with a single fluorophore (6-carboxyfluorescein) at the 3'-end of the processed strand was shown to be a useful tool for monitoring DNA-binding and 3'-processing activities of HIV-1 and PFV integrases (INs). The DNA binding to either of the INs resulted in a fluorescence signa...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.06.009
更新日期:2012-11-01 00:00:00
abstract::The eosinophil cationic protein (ECP) is a human antimicrobial protein involved in the host immune defense that belongs to the pancreatic RNase A family. ECP displays a wide range of antipathogen activities. The protein is highly cationic and its bactericidal activity is dependant on both cationic and hydrophobic surf...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.10.005
更新日期:2011-02-01 00:00:00
abstract::The physical maps of cloned recBCD gene regions of Serratia marcescens and Proteus mirabilis were correlated to genes located in this region. The genes thyA, recC, recB, recD and argA were organized as in Escherichia coli. The 3 rec genes code for the 3 different subunits of the RecBCD enzyme and produced enzymes prom...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90104-9
更新日期:1991-04-01 00:00:00
abstract::Evidence shows that oral glycerol supplementation during the early stages of rat liver cancer reduces the growth of preneoplastic lesions. Besides, human hepatocellular carcinoma (HCC) cells display decreased expression of glycerol channel aquaporin 9 (AQP9) and also diminished glycerol-3-phosphate (G3P) content. Acco...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.01.002
更新日期:2020-03-01 00:00:00
abstract::A soluble hydrogenase from the halophilic sulfate reducing bacterium Desulfovibrio salexigens, strain British Guiana (NCIB 8403) has been purified to apparent homogeneity with a final specific activity of 760 mumoles H2 evolved/min/mg (an overall 180-fold purification with 20% recovery yield). The enzyme is composed o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(86)81071-9
更新日期:1986-01-01 00:00:00
abstract::Due to their function as adapters in translation, tRNA molecules share a common structural organization in all kingdoms and organelles with ribosomal protein biosynthesis. A typical tRNA has a cloverleaf-like secondary structure, consisting of acceptor stem, D-arm, anticodon arm, a variable region, and T-arm, with an ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.07.034
更新日期:2014-05-01 00:00:00
abstract::A thrombin-like enzyme, named BjussuSP-I, isolated from Bothrops jararacussu snake venom, is an acidic single-chain glycoprotein with M(r)=61,000, pI approximately 3.8 and 6% sugar. BjussuSP-I shows high proteolytic activity upon synthetic substrates, such as S-2238 and S-2288. It also shows procoagulant and kallikrei...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.10.005
更新日期:2008-03-01 00:00:00
abstract::The mitochondria have arisen as a consequence of endosymbiosis of an ancestral α-proteobacterium with a methane-producing archae. The main function of the canonical aerobic mitochondria include ATP generation via oxidative phosphorylation, heme and phospholipid synthesis, calcium homeostasis, programmed cell death, an...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2013.11.018
更新日期:2014-05-01 00:00:00
abstract::The chemical carcinogen N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) induces frameshift mutations located within two types of specific sequences (mutation hot spots): i) contiguous guanine sequences and ii) alternating GC sequences. The genetic requirements of these frameshift events were investigated using specific ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90117-j
更新日期:1991-04-01 00:00:00
abstract::Chronic hypobaric hypoxia induced muscle atrophy results in decreased physical performance at high altitude. Curcumin has been shown to have muscle sparing effects under cachectic conditions. However, the protective effects of curcumin under chronic hypobaric hypoxia have not been studied till now. Therefore, the pres...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.10.012
更新日期:2019-01-01 00:00:00
abstract::Affinity chromatography on cross-linked starch affords a simple and rapid procedure for alpha-amylases (EC 3.2.1.1.) purification. When starch is cross-linked in alkaline medium by epichlorhydrin in the conditions described, the insoluble polysaccharide obtained is able to retain specifically the alpha-amylase which i...
journal_title:Biochimie
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::Microalgae are single-cell, photosynthetic organisms whose biodiversity places them at the forefront of biological producers of high-value molecules including lipids and pigments. Some of these organisms particular are capable of synthesizing n-3 very long chain polyunsaturated fatty acids (VLC-PUFAs), known to have b...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2020.04.022
更新日期:2020-11-01 00:00:00
abstract::The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80316-7
更新日期:1977-01-01 00:00:00
abstract::The study of modified nucleoside contributions to RNA chemistry, structure and function has been thwarted by the lack of a site-selected method of incorporation which is both versatile and adaptable to present synthetic technologies. A reproducible and versatile site-selected incorporation of nine differently modified...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)88115-6
更新日期:1995-01-01 00:00:00
abstract::Nitrogen fixation in A. vinelandii and R. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the ATP/ADP-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. Although we have been successful in preparing in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(78)80820-7
更新日期:1978-01-01 00:00:00
abstract::The measurement and potential technological significance of in vivo missense errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the alpha subu...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(91)90192-4
更新日期:1991-12-01 00:00:00
abstract::Some oxysterols resulting either from enzymatic oxidation or autoxidation of cholesterol are associated with age-related diseases including neurodegenerative diseases. Among these oxysterols, 7β-hydroxycholesterol (7β-OHC) is often found at increased levels in patients. It is therefore important to identify molecules ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.06.027
更新日期:2018-10-01 00:00:00
abstract::L-aromatic aminoacid decarboxylase has been purified more than thousand times from homogenates of rat brain, in several steps : centrifugation, DEAE-cellulose, CM cellulose, hydroxylapatite, DEAE sephadex. Its properties have been studied, most of them on an intermediate fraction of the purification, because of the in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(75)80356-7
更新日期:1975-01-01 00:00:00
abstract::Glycolipids constitute a class of molecules with various biological activities. Among them, sugar-based bolaamphiphiles characterized by their biocompatibility, biodegradability and lower toxicity, became interesting for the development of efficient and low cost lipid-based drug delivery systems. Their activity seems ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2016.04.001
更新日期:2016-11-01 00:00:00
abstract::A discriminating system capable of recognizing the oxygenated sterols was investigated in human lymphocytes. After labelling entire cells with 25-hydroxy [3H] cholesterol (10 nM) the cytosol was ultracentrifuged on a linear sucrose density gradient. Bound 25-hydroxy [3H] cholesterol was located in a single peak with a...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80437-9
更新日期:1982-05-01 00:00:00
abstract::Nucleic acid Aptamers are ligands that are selected by a process of molecular evolution to bind with high affinities and specificities to a specific target. Recently, an increasing number of aptamers have been selected against biomarkers expressed at the surface of human cells or infectious pathogens. This class of ta...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.02.025
更新日期:2012-07-01 00:00:00
abstract::Colorectal cancer (CRC) is the third most diagnosed cancer in the western world, affecting 1 out of approximately 22 people in their lifetime. Several epidemiological studies suggest a positive association between high plasma cholesterol levels and colorectal cancer. However, the molecular mechanisms by which choleste...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.07.006
更新日期:2018-10-01 00:00:00
abstract::The oxidation of beta-D-glucose with glucose oxidase generally requires oxygen, which, under normal conditions is present at low concentrations in the reaction medium. Experiments show that glucose oxidase is no longer saturated by oxygen at enzyme concentrations greater than 0.4 mg.ml1. This is due to the decrease in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(75)80160-x
更新日期:1975-01-01 00:00:00
abstract::Isovaleryl-CoA dehydrogenase (IVD) catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA and the transfer of electrons to the electron transfer flavoprotein (ETF). Recombinant human IVD purifies with bound CoA-persulfide. A modified purification protocol was developed to isolate IVD without bound CoA-pers...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.11.007
更新日期:2015-01-01 00:00:00
abstract::A DNA sequence encoding a protein with predicted EF-hand and dynein light chain binding domains was identified in a Fasciola hepatica EST library. Sequence analysis of the encoded protein revealed that the most similar known protein was the Fasciola gigantica protein FgCaBP3 and so this newly identified protein was na...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.10.027
更新日期:2013-04-01 00:00:00
abstract::Split luciferase complementary assay has been used to investigate the effect of WD domain deletion on Apaf-1 oligomerization. Apaf-1 is an adaptor molecule in formation of apoptosome that activates caspase-9, an activation that is a key event in the mitochondrial cell death pathway. Structural studies suggest that nor...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.10.013
更新日期:2021-01-01 00:00:00