Abstract：
:A DNA sequence encoding a protein with predicted EF-hand and dynein light chain binding domains was identified in a Fasciola hepatica EST library. Sequence analysis of the encoded protein revealed that the most similar known protein was the Fasciola gigantica protein FgCaBP3 and so this newly identified protein was named FhCaBP3. Molecular modelling of FhCaBP3 predicted a highly flexible N-terminal region, followed by a domain containing two EF-hand motifs the second of which is likely to be a functioning divalent ion binding site. The C-terminal domain of the protein contains a dynein light chain like region. Interestingly, molecular modelling predicts that calcium ion binding to the N-terminal domain destabilises the β-sheet structure of the C-terminal domain. FhCaBP3 can be expressed in, and purified from, Escherichia coli. The recombinant protein dimerises and the absence of calcium ions appeared to promote dimerisation. Native gel shift assays demonstrated that the protein bound to calcium and manganese ions, but not to magnesium, barium, zinc, strontium, nickel, copper or cadmium ions. FhCaBP3 interacted with the calmodulin antagonists trifluoperazine, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide and chlorpromazine as well as the myosin regulatory light chain-binding drug praziquantel. Despite sequence and structural similarities to other members of the same protein family from F. hepatica, FhCaBP3 has different biochemical properties to the other well characterised family members, FH22 and FhCaBP4. This suggests that each member of this trematode calcium-binding family has discrete functional roles within the organism.
journal_name
Biochimiejournal_title
Biochimieauthors
Banford S,Drysdale O,Hoey EM,Trudgett A,Timson DJdoi
10.1016/j.biochi.2012.10.027subject
Has Abstractpub_date
2013-04-01 00:00:00pages
751-8issue
4eissn
0300-9084issn
1638-6183pii
S0300-9084(12)00443-9journal_volume
95pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Bacillus subtilis strain SO113 secretes a pectate lyase which is produced during the exponential death phase of growth, just before sporulation. This extracellular pectate lyase, which produces unsaturated products from polygalacturonate, was purified 35-fold from the culture supernatant of Bacillus subtilis by a CM S...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(90)90053-j
更新日期：1990-09-01 00:00:00
abstract：:The two subunits of the nickel-iron hydrogenase from Desulfovibrio gigas have been purified by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis and their amino acid compositions have been determined. The N-terminal sequences for 15 residues of the large subunit (Mr 62,000) and 25 residues of the s...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90070-3
更新日期：1988-02-01 00:00:00
abstract：:Triple-negative breast cancer (TNBC) is a heterogeneous group of breast cancer with one common feature: distinctly metastatic nature with higher rate of relapse and shorter survival compared with other subtypes of breast cancer. The epithelial to mesenchymal transition (EMT) is highly associated with cancer metastasis...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.03.004
更新日期：2019-07-01 00:00:00
abstract：:The Escherichia coli Fis protein is a dimeric DNA-binding protein whose specific binding sites share a weak consensus sequence. Use of the gel retardation technique indicates that binding of Fis on a linear DNA fragment leads to the formation of a ladder of defined retarded complexes, independently of the presence of ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(94)90021-3
更新日期：1994-01-01 00:00:00
abstract：:This review article aims to gather all the knowledge on two important proteins associated with Hevea brasiliensis rubber particles: namely the rubber elongation factor (REF) and the small rubber particle protein (SRPP). It covers more then three decades of research on these two proteins and their homologues in plants,...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2014.07.002
更新日期：2014-11-01 00:00:00
abstract：:The interactions of 6-8 ring hairpin polyamides (PAs) with the minor groove of DNA have been investigated extensively. More recent studies of large antiviral PAs (14-20 rings) active against small DNA tumor viruses lead to questions regarding the extent to which the DNA binding behaviors of the well studied, smaller P...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.07.021
更新日期：2019-10-01 00:00:00
abstract：:1) Isonitrile derivatives of synthetic polyamides, polyesters and polyalcohols, of polysaccharides such as cellulose, cross-linked dextran and agarose, linear dextran and of cross-linked and linear polyacrylamide were prepared. 2) Proteins and low-molecular-weight ligands were bound covalently to these materials by fo...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80172-6
更新日期：1980-01-01 00:00:00
abstract：:Although a 30 kDa polypeptide frequently is seen by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of post mortem (pm) skeletal muscle and in turn is used as an indicator of proteolysis, its origin has not been conclusively identified. We used antibodies to selected myofibrillar proteins...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(94)90110-4
更新日期：1994-01-01 00:00:00
abstract：:A previous study has shown the effect of individual mineral surfaces on the activity of sweet almond beta-D-glucosidase. We now consider more complex situations likely to occur in soil, such as adsorption onto mixtures of different mineral surfaces, and the effect on enzyme activity of mineral surfaces with organic co...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90197-0
更新日期：1987-06-01 00:00:00
abstract：:Cysteine-rich secretory proteins (CRISPs), characterized by 16 conserved cysteines, are distributed in a wide range of organisms, such as secernenteas, amphibians, reptiles and mammals. In the previous studies, a novel CRISP family member (cysteine-rich buccal gland protein, CRBGP) was separated from the buccal gland ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.11.020
更新日期：2016-04-01 00:00:00
abstract：:The action of indomethacin on the lactogenic activity of prolactin has been evaluated usind the technique of rabbit mammary gland organ culture. Indomethacin is totally unable to inhibit prolactin action as estimated by lactose synthetase activity and casein synthesis. These data suggest, as opposed to previous works...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80060-5
更新日期：1980-01-01 00:00:00
abstract：:Theoretical and experimental investigations on the conformations of synthetic polytripeptides, with Gly-R2-R3 general repetitive sequence, are of great interest for the comprehension of the factors which contribute to the triple helix stabilization. Using appropriate molecules as models, we have investigated by the PC...
journal_title：Biochimie
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
abstract：:mRNAs extracted from human pheochromocytoma were translated in vitro in a lysate of a rabbit reticulocytes. Two enzymes of the biosynthetic pathway of the catecholamines, tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH), were characterized as translation products after immunoprecipitation by specific anti...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80198-x
更新日期：1985-06-01 00:00:00
abstract：:Thraustochytrium is a marine protist producing a specific profile of nutritionally important fatty acids, including very long chain polyunsaturated fatty acids (VLCPUFAs) docosahexaenoic acid (DHA, 22:6n-3), even chain saturated fatty acids (SFAs) palmitic acid (16:0), and odd chain SFAs pentadecanoic acid (15:0). To ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.10.024
更新日期：2018-01-01 00:00:00
abstract：:The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central ner...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.12.008
更新日期：2020-03-01 00:00:00
abstract：:Histidine-proline-rich glycoprotein (HPRG), or histidine-rich glycoprotein (HRG), is a serum protein that is synthesized in the liver and is actively internalised by different cells, including skeletal muscle. The multidomain arrangement of HPRG comprises two modules at the N-terminus that are homologous to cystatin b...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.09.024
更新日期：2015-11-01 00:00:00
abstract：:Parkinson's disease (PD), a neurodegenerative disorder, is characterized by a loss of dopaminergic neurons in the substantia nigra (SN) of the brain and it is well known that the pathogenesis of PD is related to a number of risk factors including oxidative stress. Antioxidant 1 (ATOX1) protein plays a crucial role in ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.10.010
更新日期：2019-01-01 00:00:00
abstract：:Recent work has shown that a DBF4 analog in yeast may be a target of nitrogen-containing bisphosphonates. DBF4 is an essential protein kinase required for DNA replication from primary eukaryotes to humans and appears to play a critical role in the S-phase checkpoint. It is also required for cell migration and cell sur...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.11.010
更新日期：2014-04-01 00:00:00
abstract：:We describe a method to compare two fluorescence anisotropy decay curves. After numerical deconvolution of both decays by a non a priori method [1], their difference, D, is considered. The variance is computed for each point of D. A confidence interval is defined which allows a decision to be made as to the significan...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(84)90117-2
更新日期：1984-09-01 00:00:00
abstract：:About 150 participants attended the symposium organised at the Palais de la Bourse in Bordeaux, France on September 22-23, 2017. Thirty speakers from all over the world delivered lectures covering selection processes, aptamer chemistry and innovative applications of these powerful tools that display major advantages o...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.11.015
更新日期：2018-02-01 00:00:00
abstract：:We have studied through the late foetal period of the rat, the evolution of two enzymes involved in glycogen metabolism of the liver : UDPG glycogen synthetase (a and b forms) and branching enzyme. The activities were measured during the development of normal foetuses and of foetuses experimentally deprived of cortico...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(75)80140-4
更新日期：1975-01-01 00:00:00
abstract：:The Escherichia coli protein synthesis initiation factor IF2 is a member of the large family of G-proteins. Along with translational elongation factors EF-Tu and EF-G and translational release factor RF-3, IF2 belongs to the subgroup of G-proteins that are part of the prokaryotic translational apparatus. The roles of ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(00)01200-1
更新日期：2000-12-01 00:00:00
abstract：:The formation of integral membrane voltage-gated ion channels by the initially soluble C-terminal channel polypeptide (CP) of the pore-forming colicins is a fruitful area for studies on membrane protein import. The dependence of CP import on specific membrane parameters can be better understood using liposomes and pla...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(02)01453-0
更新日期：2002-05-01 00:00:00
abstract：:The amino acid sequence of rat thymus histone obtained in highly purified form by preparative electrophoresis, was determined. This sequence is identical to the sequence of calf thymus histone H2B. The in vitro phosphorylation of the rat histone with a cyclic AMP-dependent protein kinase isolated from rat pancreas led...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(79)80313-2
更新日期：1979-01-01 00:00:00
abstract：:DNA mismatch repair (MMR) is an important replication error avoidance mechanism that prevents mutation. The association of defective MMR with familial and sporadic gastrointestinal and endometrial cancer has been acknowledged for some years. More recently, it has become apparent that MMR defects are common in acute my...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2003.10.007
更新日期：2003-11-01 00:00:00
abstract：:Vegetable oils, consisting principally of triacylglycerols (TAG), are major sources of calories and essential fatty acids in the human diet. The fatty acid composition of TAG is a primary determinant of the nutritional quality and health-promoting properties of vegetable oils. TAG fatty acid composition also affects t...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2020.09.020
更新日期：2020-11-01 00:00:00
abstract：:DNA replication in Escherichia coli is controlled at the initiation stage, possibly by regulation of the essential activity of DnaA protein. The cellular membrane has long been hypothesized to be involved in chromosomal replication. Accumulating evidence, both in vitro and in vivo, that supports the importance of memb...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(00)01224-4
更新日期：2001-01-01 00:00:00
abstract：:Bothropstoxin-I (BthTx-I) is a homodimeric Lys49-PLA2 from the venom of the snake Bothrops jararacussu, which lacks hydrolytic activity against phospholipid substrates, yet permeabilizes membranes by a Ca2+-independent mechanism. The interaction of the BthTx-I with model membranes has been studied by intrinsic tryptop...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.10.012
更新日期：2008-09-01 00:00:00
abstract：:The comparison of the amino acid sequences of 5 cysteine proteinases: papain, actinidin, rat cathepsins B and H and chicken cathepsin L, demonstrates a striking homology among their sequences. The N-terminal region (residues 1-70 in papain) and C-terminal region (residues 118-212 in papain) display the highest sequenc...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(88)90004-1
更新日期：1988-10-01 00:00:00
abstract：:Nitrogen fixation in A. vinelandii and R. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the ATP/ADP-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. Although we have been successful in preparing in...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(78)80820-7
更新日期：1978-01-01 00:00:00
