Abstract:
:We describe a method to compare two fluorescence anisotropy decay curves. After numerical deconvolution of both decays by a non a priori method [1], their difference, D, is considered. The variance is computed for each point of D. A confidence interval is defined which allows a decision to be made as to the significance of D. Information on the time range in which the changes of the fluorescence anisotropy decay occur is directly available. This analysis is particularly well suited for following the perturbations induced by an effector. It has been tested on 3-phosphoglycerate kinase in the presence and in the absence of ATP and 3-phosphoglycerate. We consider that this method leads to a significant improvement in the application of time resolved depolarization experiments.
journal_name
Biochimiejournal_title
Biochimieauthors
Genest D,Privat JPdoi
10.1016/0300-9084(84)90117-2subject
Has Abstractpub_date
1984-09-01 00:00:00pages
631-8issue
9-10eissn
0300-9084issn
1638-6183pii
0300-9084(84)90117-2journal_volume
66pub_type
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