A method for deciding whether two experimental fluorescence anisotropy decay curves are significantly different.

Abstract:

:We describe a method to compare two fluorescence anisotropy decay curves. After numerical deconvolution of both decays by a non a priori method [1], their difference, D, is considered. The variance is computed for each point of D. A confidence interval is defined which allows a decision to be made as to the significance of D. Information on the time range in which the changes of the fluorescence anisotropy decay occur is directly available. This analysis is particularly well suited for following the perturbations induced by an effector. It has been tested on 3-phosphoglycerate kinase in the presence and in the absence of ATP and 3-phosphoglycerate. We consider that this method leads to a significant improvement in the application of time resolved depolarization experiments.

journal_name

Biochimie

journal_title

Biochimie

authors

Genest D,Privat JP

doi

10.1016/0300-9084(84)90117-2

subject

Has Abstract

pub_date

1984-09-01 00:00:00

pages

631-8

issue

9-10

eissn

0300-9084

issn

1638-6183

pii

0300-9084(84)90117-2

journal_volume

66

pub_type

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