Amino acid sequence of rat thymus histone H2B and identification of the in vitro phosphorylation sites.

abstract：:Conformational flexibility and structural fluctuations play an important role in enzyme activity. A great variety of internal motions ranging over different time scales and of different amplitudes are involved in the catalytic cycle. These different types of motions and their functional consequences are considered in ...

journal_title：Biochimie

authors： Yon JM,Perahia D,Ghélis C

更新日期：1998-01-01 00:00:00

abstract：:A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering...

journal_title：Biochimie

authors： Mizushina Y,Takeuchi T,Hada T,Maeda N,Sugawara F,Yoshida H,Fujita M

更新日期：2008-06-01 00:00:00

abstract：:The hepatitis E virus (HEV) is the most common cause of acute hepatitis worldwide. Although HEV is a small, naked RNA virus, HEV particles become associated with lipids in the blood of infected patients and in the supernatant of culture systems. The egress of these particles from cells implies the exocytosis pathway b...

journal_title：Biochimie

authors： Chapuy-Regaud S,Dubois M,Plisson-Chastang C,Bonnefois T,Lhomme S,Bertrand-Michel J,You B,Simoneau S,Gleizes PE,Flan B,Abravanel F,Izopet J

更新日期：2017-10-01 00:00:00

abstract：:At the first symptoms of organisation of the silkgland in the embryo, mitoses stop and nuclei start to grow. Through autoradiographic studies, performed after sequenced labeling with [3H] and [14C] thymidine, the durations of the different phases of DNA synthesis cycles (T = 42 to 48 h, S = 22 to 25 h, G = 20 to 23 h)...

journal_title：Biochimie

authors： Perdrix-Gillot S

更新日期：1979-01-01 00:00:00

abstract：:Stereospecific hydrogenations according to the general scheme [formula: see text] are of interest from a preparative and mechanistic point of view. Proteus mirabilis is suitable for the hydrogenation of a-keto-acids to R-hydroxy-acids, and a Clostridium strain for the reduction of enoates. Both have been immobilized i...

journal_title：Biochimie

authors： Tischer W,Tiemeyer W,Simon H

更新日期：1980-01-01 00:00:00

abstract：:Elongation factor 1 alpha (EF-1 alpha) was purified to homogeneity from full-grown oocytes of Xenopus laevis. This protein is encoded by a gene previously shown to be expressed in male and female germ cells, and repressed in somatic cells. The purified protein was identified with EF-1 alpha on criteria of molecular ma...

journal_title：Biochimie

authors： Morales J,Mulner-Lorillon O,Denis H,Bellé R

更新日期：1991-09-01 00:00:00

abstract：:In the intestine, NF-κB is the main transcription factor involved in the anti-inflammatory effect of glutamine and we previously demonstrated that glutamine via its conversion to glutamate diminished the p65 protein content in Caco-2/TC7 cell nuclei without affecting the stimulating effect of IL-1β on NF-κB [21]. Howe...

journal_title：Biochimie

authors： Lesueur C,Bôle-Feysot C,Bekri S,Husson A,Lavoinne A,Brasse-Lagnel C

更新日期：2012-03-01 00:00:00

abstract：:SR-PSOX can function as a scavenger receptor, a chemokine and an adhesion molecule, and it could be an interesting player in the formation of atherosclerotic lesions. Our previous studies demonstrated that basic amino acid residues in the chemokine domain of SR-PSOX are critical for its functions. In this study the co...

journal_title：Biochimie

authors： Liu W,Yin L,Dai Y

更新日期：2013-02-01 00:00:00

abstract：:Innate immune system is the first line of host defense against invading microorganisms. It relies on a limited number of germline-encoded pattern recognition receptors that recognize conserved molecular structures of microbes, referred to as pathogen-/microbe-associated molecular patterns (PAMPs/MAMPs). Bacterial cell...

journal_title：Biochimie

authors： Ray A,Cot M,Puzo G,Gilleron M,Nigou J

更新日期：2013-01-01 00:00:00

abstract：:Seven virulent bacteriophages of Streptococcus thermophilus were characterized at the molecular level and classified into 2 subgroups (A and B) by DNA/DNA hybridization experiments and analysis of their structural proteins. Two representatives of subgroups A and B were compared to 3 representatives of Neve's subgroups...

journal_title：Biochimie

authors： Benbadis L,Faelen M,Slos P,Fazel A,Mercenier A

更新日期：1990-12-01 00:00:00

abstract：:To elucidate the metabolic function of mRNA polyadenylation in Escherichia coli. we searched for a polyadenylate-binding protein as a potential mediator of the function of the poly(A) moiety. Using a nitrocellulose filter-binding assay and a Northwestern blot technique, a protein in the ribosomal supernatant fraction ...

journal_title：Biochimie

authors： Kalapos MP,Paulus H,Sarkar N

更新日期：1997-09-01 00:00:00

abstract：:We have studied through the late foetal period of the rat, the evolution of two enzymes involved in glycogen metabolism of the liver : UDPG glycogen synthetase (a and b forms) and branching enzyme. The activities were measured during the development of normal foetuses and of foetuses experimentally deprived of cortico...

journal_title：Biochimie

authors： Gilbert M,Vaillant R

更新日期：1975-01-01 00:00:00

abstract：:Esterases and lipases both hydrolyse ester bonds. Whereas the lipases display high activity towards the aggregated state of its substrate, the esterases typically show highest activity towards the soluble state of its substrate. We have compared the amino acid sequence, the 3D-structure as well as the pH-dependent ele...

journal_title：Biochimie

authors： Fojan P,Jonson PH,Petersen MT,Petersen SB

更新日期：2000-11-01 00:00:00

abstract：:TcrX/Y is one of the twelve two component system (TCS) present in Mycobacterium tuberculosis. We have investigated the TcrX/Y interaction by in silico studies, pull down assay, radioactive phosphotransfer, surface plasmon resonance as well as crosstalk analysis of TcrY with TcrA - a non-cognate response regulator. Seq...

journal_title：Biochimie

authors： Bhattacharya M,Biswas A,Das AK

更新日期：2010-03-01 00:00:00

abstract：:All phytopathogenic fungi have two catalase-peroxidase paralogues located either intracellularly (KatG1) or extracellularly (KatG2). Here, for the first time a secreted bifunctional, homodimeric catalase-peroxidase (KatG2 from the rice blast fungus Magnaporthe grisea) has been produced heterologously with almost 100% ...

journal_title：Biochimie

authors： Zámocký M,Droghetti E,Bellei M,Gasselhuber B,Pabst M,Furtmüller PG,Battistuzzi G,Smulevich G,Obinger C

更新日期：2012-03-01 00:00:00

abstract：:Alpha 1, 2-fucosyltransferase (FUT 1/2) is a rate-limiting enzyme that catalyzes the synthesis of Lewis y, a cell membrane-associated carbohydrate antigen. In human ovarian cancer, the upregulated expression of FUT1 and Lewis y is associated with advanced pathological stages and involved in cell proliferation, migrati...

journal_title：Biochimie

authors： Gao N,Liu J,Liu D,Hao Y,Yan L,Ma Y,Zhuang H,Hu Z,Gao J,Yang Z,Shi H,Lin B

更新日期：2014-12-01 00:00:00

abstract：:The possibility that brown adipose tissue thermogenesis can be recruited in order to combat the development of obesity has led to a high interest in the identification of "browning agents", i.e. agents that increase the amount and activity of UCP1 in brown and brite/beige adipose tissues. However, functional analysis ...

journal_title：Biochimie

authors： Kalinovich AV,de Jong JM,Cannon B,Nedergaard J

更新日期：2017-03-01 00:00:00

abstract：:As part of a project aimed at obtaining selective inhibitors and drug-like compounds targeting tRNA synthetases from trypanosomatids, we have elucidated the crystal structure of human cytosolic histidyl-tRNA synthetase (Hs-cHisRS) in complex with histidine in order to be able to compare human and parasite enzymes. The...

journal_title：Biochimie

authors： Koh CY,Wetzel AB,de van der Schueren WJ,Hol WG

更新日期：2014-11-01 00:00:00

abstract：UNLABELLED:Pancreatic cancer (PC) is one of the most lethal human malignancies and a major health problem. Patients diagnosed with PC and treated with conventional approaches have an overall 5-year survival rate of less than 5%. Novel strategies are needed to treat this disease. Herein, we propose a combinatorial strat...

journal_title：Biochimie

authors： Breton CS,Aubry D,Ginet V,Puyal J,Heulot M,Widmann C,Duchosal MA,Nahimana A

更新日期：2015-09-01 00:00:00

abstract：:Isolation and properties of B. subtilis ts 19 mutant, isolated as thermosensitive for sporulation, are described. At the non permissive temperature (42degreesC), the mutant cells are blocked at stage zero of sporulation and do not excrete extracellular enzymes such as serylprotease and esterase. At the permissive temp...

journal_title：Biochimie

authors： Millet J,Larribe M,Aubert JP

更新日期：1976-01-01 00:00:00

abstract：:The inhibition rates and spectral characteristics of 2 probes specific for the active-site serine residue of proteases were examined for evidence of conformational change of the proteases upon their binding to alpha 2-macroglobulin (alpha 2M). Elastase, chymotrypsin, trypsin, and plasmin were reacted with (7-nitrobenz...

journal_title：Biochimie

authors： Martini JL,Pochon F

更新日期：1989-03-01 00:00:00

abstract：:Colorectal cancer (CRC) is the third most diagnosed cancer in the western world, affecting 1 out of approximately 22 people in their lifetime. Several epidemiological studies suggest a positive association between high plasma cholesterol levels and colorectal cancer. However, the molecular mechanisms by which choleste...

journal_title：Biochimie

authors： Warns J,Marwarha G,Freking N,Ghribi O

更新日期：2018-10-01 00:00:00

abstract：:A 3-D model of the tyrosylable tRNA-like domain of the genomic brome mosaic virus RNAs was built by computer modelling based on solution probing of the molecule with different chemical and enzymatic reagents. This model encompasses four major structural domains, including two peculiar substructures oriented perpendicu...

journal_title：Biochimie

authors： Felden B,Florentz C,Westhof E,Giegé R

更新日期：1993-01-01 00:00:00

abstract：:Estradiol-PAB-cellulose, an easily prepared adsorbent, has been proposed to purify the uterine estradiol receptor according to the principle of biospecific affinity chromatography. It apparently removes all hormone binding sites when cytosol preparations are incubated with it. A systematic study of this adsorbent was ...

journal_title：Biochimie

authors： Best-Belpomme M,Richard-Foy H,Secco-Millet C,Baulieu EE

更新日期：1976-01-01 00:00:00

abstract：:Fluorescence techniques were used to detect changes in the conformation of tRNA(Phe) that may occur during the peptidyl transferase reaction in which the tRNA appears to move between binding sites on ribosomes. Such a conformational change may be a fundamental part of the translocation mechanism by which tRNA and mRNA...

journal_title：Biochimie

authors： Odom OW,Hardesty B

更新日期：1987-09-01 00:00:00

abstract：:mRNAs extracted from human pheochromocytoma were translated in vitro in a lysate of a rabbit reticulocytes. Two enzymes of the biosynthetic pathway of the catecholamines, tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH), were characterized as translation products after immunoprecipitation by specific anti...

journal_title：Biochimie

authors： Benlot C,Antreassian J,Henry JP,Legrand JC,Gros F,Thibault J

更新日期：1985-06-01 00:00:00

abstract：:Various phosphonic and sulfonic glutamate analogues as well as phosphonopeptides related to glutathione were studied for their interaction with rat kidney gamma-glutamylcysteine synthetase activity. We found, in all cases, that the presence of a phosphonic group increases the affinity for the enzyme. Among the tripept...

journal_title：Biochimie

authors： Marche M,Basurko MJ,Cassaigne A

更新日期：1987-05-01 00:00:00

abstract：:Our study highlights the tight relationship between protein binding to monolayers and the phase-state of the phospholipids. Interaction of mitochondrial creatine kinase with phospholipidic membranes was analysed using a two-phase monolayer system containing anionic phospholipids under chain mismatch conditions. Monola...

journal_title：Biochimie

authors： Vernoux N,Maniti O,Marcillat O,Vial C,Granjon T

更新日期：2009-06-01 00:00:00

abstract：:After exhaustive pronase digestion, purification by gel filtration and affinity chromatography on concanavalin A, three glycopeptide fractions were obtained from rat hemopexin. Two fractions (I and II) were concanavalin A non-reactive and one (III) was concanavalin A reactive. On the basis of carbohydrate composition,...

journal_title：Biochimie

authors： Bernard N,Lombart C,Strecker G,Montreuil J,Van Halbeek H,Vliegenthart JF

更新日期：1983-03-01 00:00:00

abstract：:Hyper- (450 mOsm/l) and hypoosmotic exposure (150 mOsm/l) of Caco-2 cells, a human intestinal epithelial cell line, induced a twofold- and a fivefold increase in the production of IL-8, a constitutively expressed cytokine, respectively. This was observed both in the presence or in the absence of added proinflammatory ...

journal_title：Biochimie

authors： Hubert A,Cauliez B,Chedeville A,Husson A,Lavoinne A

更新日期：2004-08-01 00:00:00