Identification of ribosomal protein S1 as a poly(A) binding protein in Escherichia coli.

abstract：:Diacylglycerol kinase (DGK) is a lipid-metabolizing enzyme that phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). DGKδ is highly expressed in the skeletal muscle, and a decrease in DGKδ expression increases the severity of type 2 diabetes. However, the role of DGKδ in myogenic differentiation is st...

journal_title：Biochimie

authors： Sakai H,Murakami C,Matsumoto KI,Urano T,Sakane F

更新日期：2018-08-01 00:00:00

abstract：:Catalase (E.C 1.11.1.6) was purified from leaves of Zandedeschia aethiopica to apparent homogeneity by a one-step hydrophobic interaction chromatography on a phenyl Sepharose CL-4B column. The purified enzyme preparation was obtained with a final recovery of enzyme activity of about 61% and a specific activity of 146 ...

journal_title：Biochimie

authors： Trindade H,Karmali A,Pais MS

更新日期：1988-12-01 00:00:00

abstract：:Amyloid beta protein (beta/A4) is deposited in senile plaques of patients with Alzheimer's disease. This protein is derived from a larger membrane-associated protein, termed amyloid precursor protein (APP). The constitutive processing of APP occurs at the central portion of beta/A4, resulting in the release of large N...

journal_title：Biochimie

authors： Shimokawa M,Nakamura K,Maruyama K,Tagawa K,Miyatake T,Sugita H,Ishiura S,Suzuki K

更新日期：1993-01-01 00:00:00

abstract：:Cell-free extracts of fungal mycelia of two aflatoxin non-producing isolates of Aspergillus parasiticus (SRRC 163 and SRRC 2043) were utilized for the study of enzyme activities involved in the latter stages of aflatoxin biosynthesis. The post-microsomal fractions (105,000 x g supernatant) of both SRRC 163 and SRRC 20...

journal_title：Biochimie

authors： Bhatnagar D,Cleveland TE

更新日期：1988-06-01 00:00:00

abstract：:Genetic elements coding for proteins that present amino acid identity with the conserved motifs of retroviral reverse transcriptases constitute the retroid family. With the exception of reverse transcriptases encoded by mitochondrial plasmids of Neurospora, all reverse transcriptases have an absolute requirement for a...

journal_title：Biochimie

authors： Marquet R,Isel C,Ehresmann C,Ehresmann B

更新日期：1995-01-01 00:00:00

abstract：:Nephropathy is one of the most frequent complications of chronic diabetes. The main reason for nephropathy despite being hyperglycemia, but it progresses even after good glycemic control has been achieved in diabetic patients. The effects of prior exposure to high blood glucose conditions depend upon the severity and ...

journal_title：Biochimie

authors： Kushwaha K,Sharma S,Gupta J

更新日期：2020-03-01 00:00:00

abstract：:The purpose of the present study was to investigate the effect of a concentrated preparation (EPA 30) containing eicosapentaenoic acid (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3) on the limiting desaturation steps of the polyunsaturated fatty acid biosynthesis in spontaneously hypertensive rats (SHR). Adu...

journal_title：Biochimie

authors： Narce M,Frenoux JM,Dardel V,Foucher C,Germain S,Delachambre MC,Poisson JP

更新日期：1997-02-01 00:00:00

abstract：:Some physico-chemical properties of R140G and K141Q mutants of human small heat shock protein HspB1 associated with hereditary peripheral neuropathy were analyzed. Mutation K141Q did not affect intrinsic Trp fluorescence and interaction with hydrophobic probe bis-ANS, whereas mutation R140G decreased both intrinsic fl...

journal_title：Biochimie

authors： Nefedova VV,Datskevich PN,Sudnitsyna MV,Strelkov SV,Gusev NB

更新日期：2013-08-01 00:00:00

abstract：:Isovaleryl-CoA dehydrogenase (IVD) catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA and the transfer of electrons to the electron transfer flavoprotein (ETF). Recombinant human IVD purifies with bound CoA-persulfide. A modified purification protocol was developed to isolate IVD without bound CoA-pers...

journal_title：Biochimie

authors： Mohsen AW,Vockley J

更新日期：2015-01-01 00:00:00

abstract：:The effects of DNA oligonucleotides complementary to potato spindle tuber viroid (PSTVd) on viroid infection were investigated. The oligonucleotides were used to form hybrids with PSTVd in the infection mixture. A 75% reduction of viroid infection was found when an oligonucleotide complementary to nucleotides 79-110 o...

journal_title：Biochimie

authors： Matousek J,Trnĕná L,Arnold L,Dĕdic P,Rakouský S,Steger G,Riesner D

更新日期：1993-01-01 00:00:00

abstract：:We are here reporting that calpain-1 is a constitutive component of a distinct lipid raft/caveolin-1 microdomain isolated from bEnd5 cells in association with endothelial nitric oxide synthase (eNOS) and heat shock protein 90 (HSP90). Perturbations in intracellular calcium concentration by Ca2+-ionophore A23187 or pro...

journal_title：Biochimie

authors： Martines A,Stifanese R,Faelli EL,Perasso L,Melloni I,Ruggeri P,Averna M

更新日期：2017-02-01 00:00:00

abstract：:During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...

journal_title：Biochimie

authors： Girard J,Perdereau D,Narkewicz M,Coupé C,Ferré P,Decaux JF,Bossard P

更新日期：1991-01-01 00:00:00

abstract：:Antimicrobials targeting the fatty acid synthesis (FASII) pathway are being developed as alternative treatments for bacterial infections. Emergence of resistance to FASII inhibitors was mainly considered as a consequence of mutations in the FASII target genes. However, an alternative and efficient anti-FASII resistanc...

journal_title：Biochimie

authors： Morvan C,Halpern D,Kénanian G,Pathania A,Anba-Mondoloni J,Lamberet G,Gruss A,Gloux K

更新日期：2017-10-01 00:00:00

abstract：:An immunological demonstration of alpha-fetoprotein (AFP) in uterine cytosol from immature rats is reported. The identity between serum AFP and the estrogen binding 4.5 S macromolecular complex of uterine cytosol was demonstrated by the use of a specific immunoadsorbent to AFP. Analysis of the sedimentation profile ...

journal_title：Biochimie

authors： Aussel C,Uriel J,Michel G,Baulieu EE

更新日期：1974-01-01 00:00:00

abstract：:Zika and Dengue viruses have attracted substantial attention from researchers in light of recent outbreaks of Dengue fever and increases in cases of congenital microcephaly in areas with Zika incidence. This review summarizes the current state of knowledge about Zika and Dengue proteases. These enzymes have several in...

journal_title：Biochimie

authors： Majerová T,Novotný P,Krýsová E,Konvalinka J

更新日期：2019-11-01 00:00:00

abstract：:In order to confirm the presence of a phosphoenolpyruvate (PEP)-dependent sugar-phosphotransferase system (PTS) in the mollicute Acholeplasma florum we studied the ability of cell free extracts of this organism to phosphorylate glucose and/or fructose in the presence of PEP. We also cloned and sequenced a DNA fragment...

journal_title：Biochimie

authors： Navas-Castillo J,Laigret F,Hocquellet A,Chang CJ,Bove JM

更新日期：1993-01-01 00:00:00

abstract：:We present here, a new method for coupling sulfhydryl groups (SH) to low-density lipoprotein (LDL) surface. This method uses homocysteine thiolactone (HCTL) which reacts with lysine residues in a very mild manner, and permits the selection of the number of SH bound per LDL. Under our experimental conditions (8 SH/LDL)...

journal_title：Biochimie

authors： Vidal M,Sainte-Marie J,Philippot J,Bienvenue A

更新日期：1986-05-01 00:00:00

abstract：:The wheat germ translation initiation factor 2 (WGeIF-2) was isolated in a homogeneous state by an efficient procedure and characterized. Its molecular mass, as determined by a gel-filtration method is approximately 150,000 Da. According to SDS-PAGE WGeIF-2 consists of four subunits with M(r) 37,000 (alpha), 40,000 (b...

journal_title：Biochimie

authors： Shaikhin SM,Smailov SK,Lee AV,Kozhanov EV,Iskakov BK

更新日期：1992-05-01 00:00:00

abstract：:The variable manifestation of phenotypes that occur in patients with neurofibromatosis type 1 (NF1) includes benign and malignant neurocutaneous tumors for which no adequate treatment exists. Cell-based screening of known bioactive compounds library identified the protein phosphatase 2A (PP2A) inhibitor Cantharidin an...

journal_title：Biochimie

authors： Semenova G,Stepanova DS,Deyev SM,Chernoff J

更新日期：2017-04-01 00:00:00

abstract：:Oxidatively modified proteins are continuously produced in cells by reactive oxygen and nitrogen species generated as a consequence of aerobic metabolism. During periods of oxidative stress, protein oxidation is significantly increased and may become a threat to cell survival. In eucaryotic cells the proteasome has be...

journal_title：Biochimie

authors： Davies KJ

更新日期：2001-03-01 00:00:00

abstract：:Sphingosine is a natural sphingolipid found in membranes of all eukaryotic cells. In addition to its functions in cell signaling, sphingosine has broad-spectrum antimicrobial properties. Sphingosine's role as an antimicrobial is important in tissues such as the skin and respiratory epithelium. Reduction in the normal ...

journal_title：Biochimie

authors： Martin GE,Boudreau RM,Couch C,Becker KA,Edwards MJ,Caldwell CC,Gulbins E,Seitz A

更新日期：2017-10-01 00:00:00

abstract：:Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric ...

journal_title：Biochimie

authors： Cafaro V,Bracale A,Formiggini F,Notomista E,D'Alessio G,Di Donato A

更新日期：1998-11-01 00:00:00

abstract：:Our understanding of plant meiosis is rapidly increasing thanks to the model Arabidopsis thaliana. Here we present the results of a screening for meiotic mutants carried out with a library containing 30,719 T-DNA insertion lines. An average of one mutant per 1000 lines was recovered. Several phenotypic classes could b...

journal_title：Biochimie

authors： Mercier R,Grelon M,Vezon D,Horlow C,Pelletier G

更新日期：2001-11-01 00:00:00

abstract：:The Nicotiana tabacum Nt-4/1 protein is a plant-specific protein of unknown function. Analysis of bacterially expressed Nt-4/1 protein in vitro revealed that the protein secondary structure is mostly alpha-helical and suggested that it could consist of three structural domains. Earlier studies of At-4/1, the Arabidops...

journal_title：Biochimie

authors： Solovyev AG,Minina EA,Makarova SS,Erokhina TN,Makarov VV,Kaplan IB,Kopertekh L,Schiemann J,Richert-Pöggeler KR,Morozov SY

更新日期：2013-07-01 00:00:00

abstract：:Prostaglandin E synthase (PGES), which converts cyclooxygenase (COX)-derived prostaglandin H(2) (PGH(2)) to PGE(2), is known to comprise a group of at least three structurally and biologically distinct enzymes. Two of them are membrane-bound and have been designated as mPGES-1 and mPGES-2. mPGES-1 is a perinuclear pro...

journal_title：Biochimie

authors： Hara S,Kamei D,Sasaki Y,Tanemoto A,Nakatani Y,Murakami M

更新日期：2010-06-01 00:00:00

abstract：:The exosome is a complex of eleven subunits in yeast, involved in RNA processing and degradation. Despite the extensive in vivo functional studies of the exosome, little information is yet available on the structure of the complex and on the RNase and RNA binding activities of the individual subunits. The current mode...

journal_title：Biochimie

authors： Luz JS,Tavares JR,Gonzales FA,Santos MC,Oliveira CC

更新日期：2007-05-01 00:00:00

abstract：:Glutathione, being a major intracellular redox regulator has been shown to be implicated in regulation of airway reactivity and inflammation. However, no study so far has investigated the effect of glutathione depletion/repletion during sensitization and challenge phases separately, which could provide an important in...

journal_title：Biochimie

authors： Nadeem A,Siddiqui N,Alharbi NO,Alharbi MM,Imam F,Sayed-Ahmed MM

更新日期：2014-08-01 00:00:00

abstract：:Directed mutagenesis has been used to study the nicotinamide subsite of the glycolytic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Residue Asn313 is involved together with the carboxyamide moiety of the nicotinamide ring in a complex network of hydrogen bonding interactions which fix the position o...

journal_title：Biochimie

authors： Corbier C,Mougin A,Mely Y,Adolph HW,Zeppezauer M,Gerard D,Wonacott A,Branlant G

更新日期：1990-08-01 00:00:00

abstract：:Oxidative stress, characterized by the accumulation of reactive oxygen species (ROS), is known to have numerous detrimental effects on the myocardium such as the induction of apoptotic cell death, hypertrophy, fibrosis, dysfunction, and dilatation. Over the past several years, we have shown that mitochondrial NADP(+)-...

journal_title：Biochimie

authors： Lee JH,Park JW

更新日期：2014-04-01 00:00:00

abstract：:p53 signaling plays an important role in cell death. Zerumbone, a natural cyclic sesquiterpene, has shown cytotoxic activity against many cancers. This study was done to investigate the anticancer effects of zerumbone on non-small cell lung cancer (NSCLC) cells and explored the involvement of p53 signaling. Cell viabi...

journal_title：Biochimie

authors： Hu Z,Zeng Q,Zhang B,Liu H,Wang W

更新日期：2014-12-01 00:00:00