Purification and characterization of a germ cell-specific form of elongation factor 1 alpha (EF-1 alpha) from Xenopus laevis.

abstract：:A lignin model compound, named in short guaiagylglycerol beta-guaiacyl ether (GGE), contains the beta-0-4 ether linkage that is common in the chemical structure of lignin. A Pseudomonas sp. (GU5) had been isolated as an organism able to grow with GGE as the sole source of carbon and energy. When grown on vanillate, th...

journal_title：Biochimie

authors： Pelmont J,Barrelle M,Hauteville M,Gamba D,Romdhane M,Dardas A,Beguin C

更新日期：1985-09-01 00:00:00

abstract：:Replication protein A (RPA) is a heterotrimeric single-stranded DNA binding protein whose role in DNA replication, recombination and repair has been mainly elucidated through in vitro biochemical studies utilizing the mammalian complex. However, the identification of homologs of all three subunits in Saccharomyces cer...

journal_title：Biochimie

authors： Smith J,Zou H,Rothstein R

更新日期：2000-01-01 00:00:00

abstract：:Here we report two novel 17-mer amidated linear peptides (TsAP-1 and TsAP-2) whose structures were deduced from cDNAs cloned from a venom-derived cDNA library of the Brazilian yellow scorpion, Tityus serrulatus. Both mature peptides were structurally-characterised following their location in chromatographic fractions ...

journal_title：Biochimie

authors： Guo X,Ma C,Du Q,Wei R,Wang L,Zhou M,Chen T,Shaw C

更新日期：2013-09-01 00:00:00

abstract：:Pentatricopeptide repeat (PPR) proteins are a large family of modular RNA-binding proteins which mediate several aspects of gene expression primarily in organelles but also in the nucleus. These proteins facilitate processing, splicing, editing, stability and translation of RNAs. While major advances in PPR research h...

journal_title：Biochimie

authors： Manna S

更新日期：2015-06-01 00:00:00

abstract：:The single channel data for 4 different linear gramicidins containing either 4 Trp, 4 Phe, 4 Tyr or TyrBzl have been analyzed on the basis of 3 barriers-2 sites model. They form 2 families which differ by their single channel behavior and thus different energy profiles of the channel. A relationship between the surfac...

journal_title：Biochimie

authors： Heitz F,Van Mau N,Bennes R,Daumas P,Trudelle Y

更新日期：1989-01-01 00:00:00

abstract：:The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI...

journal_title：Biochimie

authors： Krauchenco S,Nagem RA,da Silva JA,Marangoni S,Polikarpov I

更新日期：2004-03-01 00:00:00

abstract：:NDPK-A, NDPK-B and NDPK-D are three enzymes which belong to the NDPK group I isoforms and are not only involved in metabolism process but also in transcriptional regulation, DNA cleavage, histidine protein kinase activity and metastasis development. Those enzymes were reported to bind to membranes either in mitochondr...

journal_title：Biochimie

authors： Francois-Moutal L,Marcillat O,Granjon T

更新日期：2014-10-01 00:00:00

abstract：:Aptamers are short single-stranded nucleic acid molecules that are selected in vitro from a large random sequence library based on their high and specific affinity to a target molecule by a process known as SELEX. Cell-SELEX that employs whole living cells overexpressing the defined cell surface proteins (for selectio...

journal_title：Biochimie

authors： Takahashi M,Sakota E,Nakamura Y

更新日期：2016-12-01 00:00:00

abstract：:Reuber H 35 hepatoma cells were synchronized by transfer in a serum free medium. Growth was re-initiated by addition of serum. Under these conditions DNA synthesis exhibited a maximum after 24 hours. Chromatin non-histone proteins prepared from cells at various phases of the cell cycle were incubated with [gamma-32P] ...

journal_title：Biochimie

authors： Defer N,Tichonicky L,Kruh J,Zoutewelle G,Van Wijk R

更新日期：1979-01-01 00:00:00

abstract：:Human mitochondrial diseases, defined as the diseases due to a mitochondrial oxidative phosphorylation defect, represent a large group of very diverse diseases with respect to phenotype and genetic causes. They present with many unsolved issues, the comprehensive analysis of which is beyond the scope of this review. W...

journal_title：Biochimie

authors： Lombès A,Auré K,Bellanné-Chantelot C,Gilleron M,Jardel C

更新日期：2014-05-01 00:00:00

abstract：:The microbial resistance to antibiotics is a genuine global threat. Consequently, a search of new inhibitors remains of acute importance due to the increasing spread of multidrug resistance. Here we present a new type of non-β-lactam β-lactamase inhibitor PA-34 based on natural phenoxyaniline, identified using compute...

journal_title：Biochimie

authors： Grigorenko VG,Andreeva IP,Rubtsova MY,Deygen IM,Antipin RL,Majouga AG,Egorov AM,Beshnova DA,Kallio J,Hackenberg C,Lamzin VS

更新日期：2017-01-01 00:00:00

abstract：:Thyroglobulin is a large-size iodoglycoprotein specific to thyroid tissue and is the substrate for the synthesis of thyroid hormones, thyroxine and 3,5,3'-triiodothyronine. Recent studies, which greatly benefited from recombinant DNA methodologies, improved the knowledge of several structural features of this dimeric ...

journal_title：Biochimie

authors： Malthièry Y,Marriq C,Bergé-Lefranc JL,Franc JL,Henry M,Lejeune PJ,Ruf J,Lissitzky S

更新日期：1989-02-01 00:00:00

abstract：:The cellular concentration of cyclic nucleotides is largely dependent upon the activity of the enzymatic system responsible for their degradation: cyclic nucleotide phosphodiesterase. This enzymatic system thus plays a crucial role in the regulation of the multiple functions which are modulated by cyclic nucleotides i...

journal_title：Biochimie

authors： Nemoz G,Prigent AF

更新日期：1984-02-01 00:00:00

abstract：:Well-defined quantities of *OH, O2*-,HO2* or RO2*)radicals (reactive oxygen species) can be specifically produced by radiolysis of water or ethanol. Such radical species can initiate one-electron oxidation or one-electron reduction reactions on numerous biological systems. The oxidative hypothesis of atherosclerosis c...

journal_title：Biochimie

authors： Bonnefont-Rousselot D

更新日期：2004-12-01 00:00:00

abstract：:The intervention of pyruvate in glucose metabolism was investigated during hypoxic stress in tumour cell cultures having respiratory capacities under normoxic conditions. Results obtained with nuclear magnetic resonance (NMR) spectroscopy showed that, under normoxic conditions, rat glioma C6 and human hepatoma Hep G2 ...

journal_title：Biochimie

authors： Perrin A,Roudier E,Duborjal H,Bachelet C,Riva-Lavieille C,Leverve X,Massarelli R

更新日期：2002-10-01 00:00:00

abstract：:In the process of L-phenylalanine oxidation by Proteus mirabilis cytoplasmic membrane, hydrogen peroxide was produced at a rate corresponding to 1-3 per cent of the total electron flow (30-110 nmoles min-1mg-1). Peroxide was estimated using a fluorimetric assay with horseradish peroxidase, or by anodic oxidation on a ...

journal_title：Biochimie

authors： Sauret-Ignazi G,Laboure-Rossat AM,Jouve HM,Pelmont J

更新日期：1982-10-01 00:00:00

abstract：:The bovine oxytocin precursor was expressed in Escherichia coli as a fusion protein by cloning the hormone encoding cDNA in frame behind the replicase gene of the RNA phage MS2. By step-wise extraction with different urea concentrations, the fusion protein was enriched in the 7 M urea fraction and further purified by ...

journal_title：Biochimie

authors： Nörenberg U,Brunath A,Richter D

更新日期：1988-01-01 00:00:00

abstract：:P53 is a transcription factor also called the "cellular gatekeeper of genome" because it can induce cell cycle arrest in G1, apoptosis or affect DNA replication in response to DNA damage. Wild type p53 is localised in both the cytoplasm and nucleus of first trimester trophoblastic cells (CTB). Immunoblotting of CTB wi...

journal_title：Biochimie

authors： Cohen M,Wuillemin C,Bischof P

更新日期：2008-06-01 00:00:00

abstract：:Acetylcholinesterase (AChE) has been identified and purified from the venom of desert cobra (W aegyptia) to apparent homogeneity using a TSK G 3000 SW gel filtration column and a Mono Q anion-exchange column. AChE was purified to homogeneity as established by sodium dodecylsulfate/polyacrylamide gel electrophoresis. T...

journal_title：Biochimie

authors： Duhaiman AS,Alhomida AS,Rabbani N,Kamal MA,al-Jafari AA

更新日期：1996-01-01 00:00:00

abstract：:The number of proteins known to contain collagen-like triple helical domains is rapidly increasing. The functions of these domains are to provide molecular rods that separate spatially non-triple helical domains with varied properties and structures and to permit lateral interactions between molecules. Two-thirds of t...

journal_title：Biochimie

authors： van der Rest M,Garrone R

更新日期：1990-06-01 00:00:00

abstract：:Ligation of CD47 by its natural ligand thrombospondin (TSP), or cross-linking by CD47 antibodies, triggers caspase-independent cell death in normal and leukemic cells. This kind of cell death is characterised by the cytoplasmic events of apoptosis including externalisation of phosphatidylserines and mitochondria swell...

journal_title：Biochimie

authors： Roué G,Bitton N,Yuste VJ,Montange T,Rubio M,Dessauge F,Delettre C,Merle-Béral H,Sarfati M,Susin SA

更新日期：2003-08-01 00:00:00

abstract：:A higher level of N-acetylglucosamine incorporation by proteinic and polyprenic endogenous acceptors is observed after infection by Myxovirus. This phenomenon occurs in whole mitochondria as in outer mitochondrial membranes, where it is particularly obvious with proteinic endogenous acceptors. Under viral infection, n...

journal_title：Biochimie

authors： Gateau O,Morelis R,Louisot P

更新日期：1980-01-01 00:00:00

abstract：:Cell sterol supply is subjected to tight negative feedback regulation through the SREBP pathway. Upon cholesterol depletion, SREBP transcription factors become activated by cleavage of a membrane bound precursor form, which stimulates the expression of the genes encoding proteins of the cholesterol synthesis pathway. ...

journal_title：Biochimie

authors： Robichon C,Dugail I

更新日期：2007-02-01 00:00:00

abstract：:Maximum 125I-IGF-I/Sm-C total binding to chick embryo fibroblasts was 3% at +37 degrees C and decreased to less than 1% in presence of 2.8 X 10(-9) M unlabelled IGF-I/Sm-C. Insulin did not compete with IGF-I/Sm-C for the binding to cells. Biological action of IGF-I/Sm-C was evaluated on 2-deoxyglucose and alpha-aminoi...

journal_title：Biochimie

authors： Cynober L,Aussel C,Chatelain P,Vaubourdolle M,Agneray J,Ekindjian OG

更新日期：1985-10-01 00:00:00

abstract：:Present knowledge of the different enzymatic steps of the denitrification chains in various bacteria, particularly Paracoccus denitrificans and Pseudomonas aeruginosa has been briefly reviewed. The question whether nitric oxide (NO), nitrous oxide (N2O) and other nitrogen derivatives are obligatory intermediates has b...

journal_title：Biochimie

authors： Henry Y,Bessières P

更新日期：1984-04-01 00:00:00

abstract：:Versican and decorin, two proteoglycans (PGs) with contradictory roles in the pathophysiology of cancer, comprise important stromal components in many tumor types and play a crucial role in the progression of cancer. In this study, we provide direct evidence for a significant and stage-related accumulation of versican...

journal_title：Biochimie

authors： Skandalis SS,Theocharis AD,Papageorgakopoulou N,Vynios DH,Theocharis DA

更新日期：2006-09-01 00:00:00

abstract：:The mechanism responsible for creating the division site in the right place at the right time in bacteria is unknown. It has been attributed to the formation of proteolipid domains in the cytoplasmic membrane surrounding the nucleoids. We interpret the growing evidence for this hypothesis by invoking hyperstructures, ...

journal_title：Biochimie

authors： Norris V,Fishov I

更新日期：2001-01-01 00:00:00

abstract：:Insects have developed an efficient host defense against microorganisms, which involves humoral and cellular mechanisms. Numerous data highlight similarities between defense responses of insects and innate immunity of mammals. The fruit fly, Drosophila melanogaster, is a favorable model system for the analysis of the ...

journal_title：Biochimie

authors： Levy F,Rabel D,Charlet M,Bulet P,Hoffmann JA,Ehret-Sabatier L

更新日期：2004-09-01 00:00:00

abstract：:Directed mutagenesis has been used to study the nicotinamide subsite of the glycolytic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Residue Asn313 is involved together with the carboxyamide moiety of the nicotinamide ring in a complex network of hydrogen bonding interactions which fix the position o...

journal_title：Biochimie

authors： Corbier C,Mougin A,Mely Y,Adolph HW,Zeppezauer M,Gerard D,Wonacott A,Branlant G

更新日期：1990-08-01 00:00:00

abstract：:Catalase (E.C 1.11.1.6) was purified from leaves of Zandedeschia aethiopica to apparent homogeneity by a one-step hydrophobic interaction chromatography on a phenyl Sepharose CL-4B column. The purified enzyme preparation was obtained with a final recovery of enzyme activity of about 61% and a specific activity of 146 ...

journal_title：Biochimie

authors： Trindade H,Karmali A,Pais MS

更新日期：1988-12-01 00:00:00