Gamma radiolysis as a tool to study lipoprotein oxidation mechanisms.

abstract：:Three mutanase (alpha-1,3-glucanase)-producing microorganisms isolated from soil samples were identified as a relatives of Paenibacillus. A mutanase was purified to homogeneity from cultures of each, and the molecular masses of the purified enzymes were approximately 132, 141, and 141kDa, respectively. The correspondi...

journal_title：Biochimie

authors： Hakamada Y,Sumitomo N,Ogawa A,Kawano T,Saeki K,Ozaki K,Ito S,Kobayashi T

更新日期：2008-03-01 00:00:00

abstract：:Bikunin (Bk) is a Künitz-type serine proteinase inhibitor, which occurs in human plasma, mainly as covalent complexes with one or two of the three peptide heavy chains. The leading member of this glycoprotein family is inter-alpha-inhibitor (I alpha I), which consists of two heavy chains (H1 and H2) linked to Bk. Bk c...

journal_title：Biochimie

authors： Capon C,Mizon C,Lemoine J,Rodié-Talbère P,Mizon J

更新日期：2003-01-01 00:00:00

abstract：:Our principle hypothesis is that the hypothalamic hormone, gonadotropin-releasing hormone (GnRH), is an immunostimulatory hormone and plays a pivotal role in the gender differences in immunity and/or autoimmunity. As a general rule, females display heightened immune responses and heightened endocrinological responsive...

journal_title：Biochimie

authors： Ansari MA,Dhar M,Muthukrishnan V,Morton TL,Bakht N,Jacobson JD

更新日期：2003-06-01 00:00:00

abstract：:The function of glycerophosphate and malate-aspartate shuttles during glucose metabolism in two strains of Ehrlich ascites tumor cells was evaluated by several experimental approaches. The activities of the enzymes involved in these shuttle systems were assayed in the cytosolic and mitochondrial compartments after cel...

journal_title：Biochimie

authors： Sánchez-Jiménez F,Martínez P,Núñez de Castro I,Olavarría JS

更新日期：1985-02-01 00:00:00

abstract：:Cell-free extracts of fungal mycelia of two aflatoxin non-producing isolates of Aspergillus parasiticus (SRRC 163 and SRRC 2043) were utilized for the study of enzyme activities involved in the latter stages of aflatoxin biosynthesis. The post-microsomal fractions (105,000 x g supernatant) of both SRRC 163 and SRRC 20...

journal_title：Biochimie

authors： Bhatnagar D,Cleveland TE

更新日期：1988-06-01 00:00:00

abstract：:Poly(ADP-ribosyl)ation is a posttranslational protein modification significant for genomic stability and cell survival in response to DNA damage. Poly(ADP-ribosyl)ation is catalyzed by poly(ADP-ribose)polymerases (PARPs). Among the 17 members of the PARP family, PARP-1 and PARP-2 are described as enzymes whose catalyt...

journal_title：Biochimie

authors： Kutuzov MM,Khodyreva SN,Amé JC,Ilina ES,Sukhanova MV,Schreiber V,Lavrik OI

更新日期：2013-06-01 00:00:00

abstract：:It is now over 10 years since the discovery of peroxisome proliferator activated receptor gamma (PPAR gamma) and its unique role in adipogenesis. The subsequent identification of PPAR gamma as the target of insulin sensitizing drugs certified this ligand-regulated transcription factor as an exciting link between adipo...

journal_title：Biochimie

authors： Lazar MA

更新日期：2005-01-01 00:00:00

abstract：:We have purified, by a three step procedure, a yeast C-nitrosoreductase and studied its action on p-nitroso-N,N-dimethylaniline. Microcalorimetric measurements performed on the microcalorimeter LKB "batch", have been given the number of protons exchanged during the reaction : four protons are involved. Two are given b...

journal_title：Biochimie

authors： Huynh CH

更新日期：1975-01-01 00:00:00

abstract：:The rate of hydrolysis of long chain triglycerides by pure bovine pancreatic lipase has been determined in the presence of variable amounts of bile salts and colipase. Cofactor-free lipase is strongly inhibited by sodium taurodesoxycholate and by mixed bovine bile salts at concentrations higher than the critical micel...

journal_title：Biochimie

authors： Rathelot J,Julien R,Canioni P,Coeroli C,Sarda L

更新日期：1975-01-01 00:00:00

abstract：:We describe a method to compare two fluorescence anisotropy decay curves. After numerical deconvolution of both decays by a non a priori method [1], their difference, D, is considered. The variance is computed for each point of D. A confidence interval is defined which allows a decision to be made as to the significan...

journal_title：Biochimie

authors： Genest D,Privat JP

更新日期：1984-09-01 00:00:00

abstract：:Heat shock proteins (Hsps) are involved in several important aspects of the cell proteostasis. Hsp90 interacts with at least a tenth of the cell proteome helping a large number of proteins to fold correctly. Hsp90 function is modulated by several co-chaperones having TPR (tetratricopeptide repeat) domains that allow f...

journal_title：Biochimie

authors： Araujo SA,Martins GH,Quel NG,Aragão AZB,Morea EGO,Borges JC,Houry WA,Cano MIN,Ramos CHI

更新日期：2021-01-06 00:00:00

abstract：:The lactose plasmid pUCL22 of the single plasmid strain Streptococcus lactis ssp. lactis Z270 was demonstrated to fuse with the heterologous conjugative plasmid pVA797. The fusion of pUCL22 with pVA797 occurred by recombination between a specific sequence of pUCL22 and different sites of pVA797. The cointegrates of pU...

journal_title：Biochimie

authors： Novel M,Huang DC,Novel G

更新日期：1988-04-01 00:00:00

abstract：:C-type lectins are calcium-dependent sugar binding proteins and are distributed ubiquitously amongst vertebrate organisms. As part of a wider study on Australian snake venom components, we have identified and characterised a C-type lectin from the venom of Oxyuranus scutellatus (Australian coastal taipan) with mannose...

journal_title：Biochimie

authors： Earl ST,Robson J,Trabi M,de Jersey J,Masci PP,Lavin MF

更新日期：2011-03-01 00:00:00

abstract：:The Escherichia coli Fis protein is a dimeric DNA-binding protein whose specific binding sites share a weak consensus sequence. Use of the gel retardation technique indicates that binding of Fis on a linear DNA fragment leads to the formation of a ladder of defined retarded complexes, independently of the presence of ...

journal_title：Biochimie

authors： Bétermier M,Galas DJ,Chandler M

更新日期：1994-01-01 00:00:00

abstract：:Many peptide hormone and neuropeptide precursors undergo post-translational processing at mono- and/or dibasic residues. An enzymatic activity capable of processing prodynorphin at a monobasic processing site designated 'dynorphin converting enzyme' has been previously reported in rat rain and bovine pituitary. In thi...

journal_title：Biochimie

authors： Berman YL,Rattan AK,Carr K,Devi L

更新日期：1994-01-01 00:00:00

abstract：:Over the years, imino proton exchange measurements have provided a description of base-pair opening and of properties of internal motions of nucleic acids such as the individual opening of base pairs and the insensitivity of base-pair lifetimes to the nature and stability of neighboring pairs. A recent determination o...

journal_title：Biochimie

authors： Leijon M,Leroy JL

更新日期：1997-12-01 00:00:00

abstract：:The published literature contains a number of examples of normally non-cytoplasmic proteins whose transport out of the cytoplasm is not completely abolished by drastic alterations to their routing signals (signal sequences, etc). Furthermore, there are numerous examples of cytoplasmic proteins that can be routed to an...

journal_title：Biochimie

authors： Pugsley AP

更新日期：1990-02-01 00:00:00

abstract：:Human prophospholipase A2 was purified from pancreatic juice. The protein has a molecular weight of 14500 and a free N-terminal residue identified as aspartic acid (or asparagine). The amino acid composition was determined. Partial immunological identity has been obtained between human and porcine prophospholipase A2....

journal_title：Biochimie

authors： Grataroli R,De Caro A,Guy O,Amic J,Figarella C

更新日期：1981-08-01 00:00:00

abstract：:The mechanism of action on rabbit red cells of Treponema hyodysenteriae hemolysin was studied using volume analysis and release of hemoglobin. While fixation of the hemolysin on the erythrocytes is temperature independent, it appears that hemolysis is temperature dependent. The kinetics of hemolysis proceed according ...

journal_title：Biochimie

authors： Saheb SA,Lafleur L

更新日期：1980-01-01 00:00:00

abstract：:gamma-Aminobutyraldehyde dehydrogenase from Escherichia coli K-12 has been purified and characterized from cell mutants able to grow in putrescine as the sole carbon and nitrogen source. The enzyme has an Mr of 195,000 +/- 10,000 in its dimeric form with an Mr of 95,000 +/- 1,000 for each subunit, a pH optimum at 5.4 ...

journal_title：Biochimie

authors： Prieto MI,Martin J,Balaña-Fouce R,Garrido-Pertierra A

更新日期：1987-11-01 00:00:00

abstract：:A series of halomethylated derivatives of dihydrocoumarins has been found to inhibit irreversibly proteases and esterases. alpha-Chymotrypsin, subtilish, elastase are rapidly inactivated in the presence of these compounds, while trypsin, kallicrein, papain are inhibited more slowly. Esterases like acetylcholinesterase...

journal_title：Biochimie

authors： Béchet JJ,Dupaix A,Roucous C,Bonamy AM

更新日期：1977-01-01 00:00:00

abstract：:The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central ner...

journal_title：Biochimie

authors： Sarbu M,Raab S,Henderson L,Fabris D,Vukelić Ž,Clemmer DE,Zamfir AD

更新日期：2020-03-01 00:00:00

abstract：:Mammalian chymotrypsin-like serine proteases (SPs) are one of the best-studied family of enzymes with roles in a wide range of physiological processes, including digestion, blood coagulation, fibrinolysis and humoral immunity. Extracellular SPs can form cascades, in which one protease activates the zymogen of the next...

journal_title：Biochimie

authors： Veillard F,Troxler L,Reichhart JM

更新日期：2016-03-01 00:00:00

abstract：:Estrogen plays an essential role in bone formation, and estrogen modulation dysfunction is tightly associated with postmenopausal osteoporosis (PMOP). The underlying mechanisms of estrogen-mediated osteogenic differentiation have not been well defined. In this study, murine bone marrow mesenchymal stem cells were indu...

journal_title：Biochimie

authors： Gao B,Huang Q,Jie Q,Wang L,Zhang HY,Liu J,Yang L,Luo ZJ

更新日期：2015-03-01 00:00:00

abstract：:The thrombin-binding DNA aptamer (TBA) 5'-d(GGTTGGTGTGGTTGG)-3' forms a G-quadruplex that is necessary for binding to the coagulation factor thrombin. The stability of the G-quadruplex of TBA when bound to thrombin and potassium ion (K(+)) were investigated for the wild-type oligonucleotide and for mutants in which th...

journal_title：Biochimie

authors： Nagatoishi S,Isono N,Tsumoto K,Sugimoto N

更新日期：2011-08-01 00:00:00

abstract：:Snake venom from Agkistrodon acutus consists of a number of compounds which may potentially be used as drugs. However, it is hard to obtain enough pure protein for drug development. Recently, we reported expression and purification of a novel recombinant fibrinogenase which was named rFII. Here we reported for the fir...

journal_title：Biochimie

authors： Jiang W,Ma T,Su X,Qiu P,Yan G

更新日期：2009-02-01 00:00:00

abstract：:Organisms exposed to ionizing radiation (IR) undergo increases in the production of reactive oxygen species (ROS), which are determinant components in the induction of apoptosis. Sensitive to apoptosis gene (SAG) encodes a redox-inducible and apoptosis-protective antioxidant protein. This report demonstrates that the ...

journal_title：Biochimie

authors： Kim SY,Yang ES,Lee YS,Lee J,Park JW

更新日期：2011-02-01 00:00:00

abstract：:The combination of cDNA and RNA sequencing techniques has enabled determination of the complete sequence of one of the mRNAs coding for the precursor of the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris. In this 898-nucleotide-long mRNA, 540 nucleotides code for the entire 180-amino-acid-l...

journal_title：Biochimie

authors： Pinck M,Guilley E,Durr A,Hoff M,Pinck L,Fleck J

更新日期：1984-07-01 00:00:00

abstract：:Split luciferase complementary assay has been used to investigate the effect of WD domain deletion on Apaf-1 oligomerization. Apaf-1 is an adaptor molecule in formation of apoptosome that activates caspase-9, an activation that is a key event in the mitochondrial cell death pathway. Structural studies suggest that nor...

journal_title：Biochimie

authors： Noori AR,Tashakor A,Nikkhah M,Eriksson LA,Hosseinkhani S,Fearnhead HO

更新日期：2021-01-01 00:00:00

abstract：:Latices from Asclepias spp are used in wound healing and the treatment of some digestive disorders. These pharmacological actions have been attributed to the presence of cysteine proteases in these milky latices. Asclepias curassavica (Asclepiadaceae), "scarlet milkweed" is a perennial subshrub native to South America...

journal_title：Biochimie

authors： Obregón WD,Liggieri CS,Trejo SA,Avilés FX,Vairo-Cavalli SE,Priolo NS

更新日期：2009-11-01 00:00:00