Drosophila melanogaster clip-domain serine proteases: Structure, function and regulation.

abstract：:Protein 3D structures support their biological functions. As the number of protein structures is negligible in regards to the number of available protein sequences, prediction methodologies relying only on protein sequences are essential tools. In this field, protein secondary structure prediction (PSSPs) is a mature ...

journal_title：Biochimie

authors： de Brevern AG

更新日期：2020-12-01 00:00:00

abstract：:Plant serine proteases have been widely used in food science and technology as well as in medicine. In this sense, several plant serine proteases have been proposed as potential anti-coagulants and anti-platelet agents. Previously, we have reported the purification and identification of a plant serine protease from So...

journal_title：Biochimie

authors： Pepe A,Frey ME,Muñoz F,Fernández MB,Pedraza A,Galbán G,García DN,Daleo GR,Guevara MG

更新日期：2016-06-01 00:00:00

abstract：:A d-carbamoylase from Sinorhizobium morelens S-5 was purified and characterized. The enzyme was purified 189-fold to homogeneity with a yield of 19.1% by aqueous two-phase extraction and two steps of column chromatography. The enzyme is a homotetramer with a native molecular mass of 150 kDa and a subunit relative mole...

journal_title：Biochimie

authors： Wu S,Liu Y,Zhao G,Wang J,Sun W

更新日期：2006-03-01 00:00:00

abstract：:Conformational changes induced by the binding of nickel to horseradish peroxidase C (HRPC) were studied by electronic absorption spectroscopy, fluorescence spectroscopy and circular dichroism spectroscopy. Incubation of HRPC with various concentrations of Ni(2+) for 5 minutes resulted in changes in the enzyme absorpti...

journal_title：Biochimie

authors： Tayefi-Nasrabadi H,Keyhani E,Keyhani J

更新日期：2006-09-01 00:00:00

abstract：:This review discusses the regulation of a key controlling step in the initiation of protein synthesis, the binding of mRNA to ribosomes. Particular focus is given to the phosphorylation of the cap-binding factor, eIF-4E, and the role of this factor in the regulation of cell growth. ...

journal_title：Biochimie

authors： Sonenberg N

更新日期：1994-01-01 00:00:00

abstract：:The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator ...

journal_title：Biochimie

authors： Gincel E,Lancelot G,Maurizot JC,Thuong NT,Vovelle F

更新日期：1994-01-01 00:00:00

abstract：:Dyskeratosis congenita is an inherited syndrome characterised by mucocutaneous features, bone marrow failure, an increased risk of malignancy and other somatic abnormalities. There is a considerable range of clinical severity and in its occult form the disease may present as idiopathic aplastic anaemia. Genes responsi...

journal_title：Biochimie

authors： Vulliamy TJ,Dokal I

更新日期：2008-01-01 00:00:00

abstract：:The present study was conducted on CD4(+) T cells, isolated from wild type (WT) and PPARalpha(null) mice, in order to assess the mechanism of action of docosahexaenoic acid (DHA), an n-3 fatty acid, in the modulation of two transcription factors, i.e., T-bet and GATA-3, implicated in T-cell differentiation towards, re...

journal_title：Biochimie

authors： Attakpa E,Hichami A,Simonin AM,Sansón EG,Dramane KL,Khan NA

更新日期：2009-11-01 00:00:00

abstract：:Mammalian aminoacylase-1 (Acy1) participates in the breakdown of N-acetylated amino acids during intracellular protein catabolism. Acy1 is most abundantly expressed in the kidney tubular epithelium. Lately, Acy1 deficiency was identified in children with increased urinary excretion of several N-acetylamino acids. Here...

journal_title：Biochimie

authors： Lindner HA,Täfler-Naumann M,Röhm KH

更新日期：2008-05-01 00:00:00

abstract：:The elucidation of the multiple signaling cascades coupled to the TSH receptor has offered new approaches in the understanding of the pathogenesis of Graves' disease. Here we review findings showing that immunoglobulins from Graves' patients are heterogeneous, bind to different epitopes and, similarly to TSH, activate...

journal_title：Biochimie

authors： Di Cerbo A,Corda D

更新日期：1999-05-01 00:00:00

abstract：:Numerous studies have reported the beneficial effects of antioxidants in human diseases. Among their biological effects, a majority of antioxidants scavenge reactive radicals in the body, thereby reducing oxidative stress that is associated with the pathogenesis of many diseases. Antioxidant dendrimers are a new class...

journal_title：Biochimie

authors： Lee CY,Nanah CN,Held RA,Clark AR,Huynh UG,Maraskine MC,Uzarski RL,McCracken J,Sharma A

更新日期：2015-04-01 00:00:00

abstract：:Experimental Lyme arthritis provides a mouse model for exploring the development of pathology following infection of C3H mice with Borrelia burgdorferi. Infected mice develop a reliable inflammatory arthritis of the ankle joint with severity that typically peaks around two to three weeks post-infection and then underg...

journal_title：Biochimie

authors： Brown CR,Dennis EA

更新日期：2017-10-01 00:00:00

abstract：:The present review deals with the place of single chain oligonucleotide ligands (aptamers) in affinity chromatography applied to proteins. Aptamers are not the only affinity ligands available but they represent an emerging and highly promising route that advantageously competes with antibodies in immunopurification pr...

journal_title：Biochimie

authors： Perret G,Boschetti E

更新日期：2018-02-01 00:00:00

abstract：:Classic studies suggested that the common mammalian sialic acid N-glycolylneuraminic acid (Neu5Gc) is an oncofetal antigen in humans, being immunogenic in adult humans and yet apparently expressed in human fetuses and tumors. We and others have recently found that the human deficiency of Neu5Gc can be explained by an ...

journal_title：Biochimie

authors： Varki A

更新日期：2001-07-01 00:00:00

abstract：:The hbs operon of Bacillus subtilis comprises a single gene which is localized between the spoIVA and mtrA open reading frames, and is situated at 204 degrees of the standard map of B subtilis. Expression of hbs is initiated from two distinct promoters called P1 and P2. The transcription initiation sites have been map...

journal_title：Biochimie

authors： Micka B,Marahiel MA

更新日期：1992-07-01 00:00:00

abstract：:We have analyzed changes in the supercoiling of nucleoid DNA of murine fibroblasts cultured in the presence of hydralazine. The entire DNA attached to the nuclear matrix was extracted from the cells and sedimented in neutral sucrose density gradients containing ethidium bromide. Nucleoids from cells treated with hydra...

journal_title：Biochimie

authors： Weglarz L,Koceva-Chyła A

更新日期：1998-07-01 00:00:00

abstract：:The synthesis of S100 protein increases toward the end of the exponential phase of growth of clonal rat glial cells C6 in monolayer culture. Moreover the synthesis of this protein can be increased by treatment of C6 cells with the lectin succinylated concanavalin A (succinyl ConA). In order to study the relationship b...

journal_title：Biochimie

authors： Marks A,Law J,Mahony J

更新日期：1983-11-01 00:00:00

abstract：:Fluorometry using a substrate DNA labeled with a single fluorophore (6-carboxyfluorescein) at the 3'-end of the processed strand was shown to be a useful tool for monitoring DNA-binding and 3'-processing activities of HIV-1 and PFV integrases (INs). The DNA binding to either of the INs resulted in a fluorescence signa...

journal_title：Biochimie

authors： Anisenko A,Agapkina J,Zatsepin T,Yanvarev D,Gottikh M

更新日期：2012-11-01 00:00:00

abstract：:Three trends are seen in the organization of ribosomal DNA genes during evolution: 1) gradual separation and separability of the regulation of transcription of 5S and larger subunit rRNAs; 2) retention of a transcription unit containing both large and small rRNAs; and 3) clustering of genes for both 5S and 18S-28S rDN...

journal_title：Biochimie

authors： Srivastava AK,Schlessinger D

更新日期：1991-06-01 00:00:00

abstract：:Glutathione, being a major intracellular redox regulator has been shown to be implicated in regulation of airway reactivity and inflammation. However, no study so far has investigated the effect of glutathione depletion/repletion during sensitization and challenge phases separately, which could provide an important in...

journal_title：Biochimie

authors： Nadeem A,Siddiqui N,Alharbi NO,Alharbi MM,Imam F,Sayed-Ahmed MM

更新日期：2014-08-01 00:00:00

abstract：BACKGROUND:The modest efficacy of available therapies for Hepatocellular carcinoma (HCC) indicates the need to develop novel therapeutic approaches. For the proteasome inhibitor Bortezomib (BZB), potentially attractive for HCC treatment, the mechanism of action is largely unknown. The BZB effect on E2Fs and the E2Fs co...

journal_title：Biochimie

authors： Farra R,Dapas B,Baiz D,Tonon F,Chiaretti S,Del Sal G,Rustighi A,Elvassore N,Pozzato G,Grassi M,Grassi G

更新日期：2015-05-01 00:00:00

abstract：:The effect of adenosine was tested on the energetic metabolism of fed rat liver cells after isolation. The cells were incubated in a buffered saline medium with glucose (5 mM) and adenosine (1 mM) for 30 minutes at 37 degrees C. This increased the concentration of the adenylic nucleotides ATP (+57 per cent, ADP (+39 p...

journal_title：Biochimie

authors： Marchand JC,Lavoinne A,Giroz M,Matray F

更新日期：1979-01-01 00:00:00

abstract：:A thrombin-like enzyme, named BjussuSP-I, isolated from Bothrops jararacussu snake venom, is an acidic single-chain glycoprotein with M(r)=61,000, pI approximately 3.8 and 6% sugar. BjussuSP-I shows high proteolytic activity upon synthetic substrates, such as S-2238 and S-2288. It also shows procoagulant and kallikrei...

journal_title：Biochimie

authors： Sant'Ana CD,Bernardes CP,Izidoro LF,Mazzi MV,Soares SG,Fuly AL,Zingali RB,Magro AJ,Braz AS,Fontes MR,Stábeli RG,Sampaio SV,Soares AM

更新日期：2008-03-01 00:00:00

abstract：:Diacylglycerol kinase (DGK) is a lipid-metabolizing enzyme that phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). DGKδ is highly expressed in the skeletal muscle, and a decrease in DGKδ expression increases the severity of type 2 diabetes. However, the role of DGKδ in myogenic differentiation is st...

journal_title：Biochimie

authors： Sakai H,Murakami C,Matsumoto KI,Urano T,Sakane F

更新日期：2018-08-01 00:00:00

abstract：:Estradiol-PAB-cellulose, an easily prepared adsorbent, has been proposed to purify the uterine estradiol receptor according to the principle of biospecific affinity chromatography. It apparently removes all hormone binding sites when cytosol preparations are incubated with it. A systematic study of this adsorbent was ...

journal_title：Biochimie

authors： Best-Belpomme M,Richard-Foy H,Secco-Millet C,Baulieu EE

更新日期：1976-01-01 00:00:00

abstract：:A previous study has shown the effect of individual mineral surfaces on the activity of sweet almond beta-D-glucosidase. We now consider more complex situations likely to occur in soil, such as adsorption onto mixtures of different mineral surfaces, and the effect on enzyme activity of mineral surfaces with organic co...

journal_title：Biochimie

authors： Quiquampoix H

更新日期：1987-06-01 00:00:00

abstract：BACKGROUND:Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by promoting ...

journal_title：Biochimie

authors： He PP,Ouyang XP,Tang YY,Liao L,Wang ZB,Lv YC,Tian GP,Zhao GJ,Huang L,Yao F,Xie W,Tang YL,Chen WJ,Zhang M,Li Y,Wu JF,Peng J,Liu XY,Zheng XL,Yin WD,Tang CK

更新日期：2014-11-01 00:00:00

abstract：:Some oxysterols resulting either from enzymatic oxidation or autoxidation of cholesterol are associated with age-related diseases including neurodegenerative diseases. Among these oxysterols, 7β-hydroxycholesterol (7β-OHC) is often found at increased levels in patients. It is therefore important to identify molecules ...

journal_title：Biochimie

authors： Zarrouk A,Ben Salem Y,Hafsa J,Sghaier R,Charfeddine B,Limem K,Hammami M,Majdoub H

更新日期：2018-10-01 00:00:00

abstract：:Although up to 25% of glucose released into circulation in the postabsorptive state comes from renal gluconeogenesis, the regulatory mechanisms of this process are still poorly recognized, comparing to hepatic ones. The aim of the present study was to examine if hypoxia-inducible factor-1 (HIF-1) might be involved in ...

journal_title：Biochimie

authors： Owczarek A,Gieczewska K,Jarzyna R,Jagielski AK,Kiersztan A,Gruza A,Winiarska K

更新日期：2020-01-01 00:00:00

abstract：:Autotaxin (ATX) is a secreted lysophospholipase D involved in synthesis of lysophosphatidic acid (LPA), a phospholipid growth factor acting via specific receptors (LPA1R to LPA6R) and involved in several pathologies including obesity. ATX is secreted by adipocytes and contributes to circulating LPA. ATX expression is ...

journal_title：Biochimie

authors： Rancoule C,Dusaulcy R,Tréguer K,Grès S,Attané C,Saulnier-Blache JS

更新日期：2014-01-01 00:00:00