Abstract：
:The Escherichia coli Fis protein is a dimeric DNA-binding protein whose specific binding sites share a weak consensus sequence. Use of the gel retardation technique indicates that binding of Fis on a linear DNA fragment leads to the formation of a ladder of defined retarded complexes, independently of the presence of a specific site. This non-specific binding of Fis is consistent with a model where equivalent low-affinity sites on a given fragment would be bound randomly and independently of each other by consecutive Fis dimers. Evidence is presented that non-specific binding of Fis can, however, induce an apparent site-specific conformational change in the DNA. This observation is discussed in terms of a model in which each Fis:DNA complex detected in gel retardation experiments actually represents a dynamic equilibrium of a fixed number of Fis dimers distributed on the fragment.
journal_name
Biochimiejournal_title
Biochimieauthors
Bétermier M,Galas DJ,Chandler Mdoi
10.1016/0300-9084(94)90021-3subject
Has Abstractpub_date
1994-01-01 00:00:00pages
958-67issue
10-11eissn
0300-9084issn
1638-6183pii
0300-9084(94)90021-3journal_volume
76pub_type
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