Abstract:
:Ten new defensins have been isolated from seeds of Triticum kiharae and related species of the Triticum and Aegilops genera by a combination of chromatographic procedures including affinity-, size-exclusion, and reversed-phase high-performance liquid chromatography. Nine were completely sequenced and shown to represent a family of closely related peptides with highly conserved amino acid sequences. Analysis of defensin compositions in diploid A-, B-, and D-genome donors to polyploid wheat allowed us for the first time to assign most defensin-encoding genes to particular hexaploid wheat genomes.
journal_name
Biochimiejournal_title
Biochimieauthors
Odintsova TI,Egorov TA,Musolyamov AKh,Odintsova MS,Pukhalsky VA,Grishin EVdoi
10.1016/j.biochi.2006.09.009subject
Has Abstractpub_date
2007-05-01 00:00:00pages
605-12issue
5eissn
0300-9084issn
1638-6183pii
S0300-9084(06)00205-7journal_volume
89pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Innate immune system is the first line of host defense against invading microorganisms. It relies on a limited number of germline-encoded pattern recognition receptors that recognize conserved molecular structures of microbes, referred to as pathogen-/microbe-associated molecular patterns (PAMPs/MAMPs). Bacterial cell...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.06.007
更新日期:2013-01-01 00:00:00
abstract::Thyroid peroxidase is a member of a family of mammalian peroxidases that includes myeloperoxidase, lactoperoxidase, eosinophil peroxidase, and salivary peroxidase. Protein sequences showing a high degree of sequence similarity with mammalian peroxidases have recently been observed in several invertebrate species. A mu...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80110-2
更新日期:1999-05-01 00:00:00
abstract::Intracellular cytoplasmic peptidoglycan (PG) intermediate levels were determined in Staphylococcus aureus during log-phase growth in enriched media. Levels of UDP-linked intermediates were quantitatively determined using ion pairing LC-MS/MS in negative mode, and amine intermediates were quantitatively determined ster...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.11.017
更新日期:2016-02-01 00:00:00
abstract::Our study highlights the tight relationship between protein binding to monolayers and the phase-state of the phospholipids. Interaction of mitochondrial creatine kinase with phospholipidic membranes was analysed using a two-phase monolayer system containing anionic phospholipids under chain mismatch conditions. Monola...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.03.014
更新日期:2009-06-01 00:00:00
abstract::Cotranslational translocation of exportable proteins across the RER membrane prior to their release into the extracellular space has been essentially described by use of canine pancreatic microsomal membranes. Intracisternal segregation of nascent secretory proteins was observed to be irreversible and proteolytic remo...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(88)90194-0
更新日期:1988-09-01 00:00:00
abstract::Contradictory results for concentrations of vitamin B12, holotranscobalamin (holoTC), and methylmalonic acid (MMA) have been reported. We tested the hypothesis that the extracellular vitamin B12 markers are not reflecting the intracellular vitamin B12-dependent biochemical reactions in individuals with type 2 diabetes...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.10.028
更新日期:2013-05-01 00:00:00
abstract::The expression of serotonin (5-HT) in the retina was first reported in the sixties. The detection of vesicular monoamine transporter and serotonin receptors in several retinal cells confirm that 5-HT is playing a neuromodulatory role in this structure. Whereas signaling pathways activated by 5-HT receptor binding has ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2018.11.006
更新日期:2019-06-01 00:00:00
abstract::The Escherichia coli Fis protein is a dimeric DNA-binding protein whose specific binding sites share a weak consensus sequence. Use of the gel retardation technique indicates that binding of Fis on a linear DNA fragment leads to the formation of a ladder of defined retarded complexes, independently of the presence of ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90021-3
更新日期:1994-01-01 00:00:00
abstract::A soluble hydrogenase from the halophilic sulfate reducing bacterium Desulfovibrio salexigens, strain British Guiana (NCIB 8403) has been purified to apparent homogeneity with a final specific activity of 760 mumoles H2 evolved/min/mg (an overall 180-fold purification with 20% recovery yield). The enzyme is composed o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(86)81071-9
更新日期:1986-01-01 00:00:00
abstract::The chicken ovalbumin (Ov) gene is one of the best models to study tissue-specific gene regulation because it is only expressed in the oviduct. In this paper, a tissue-specific element was characterized by 5'-flanking region deletion in combination with in vivo gene electroporation (EP). Plasmids with varying lengths ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.06.015
更新日期:2006-12-01 00:00:00
abstract::Glucose-6 phosphatase (G6Pase), a key enzyme of glucose homeostasis, catalyses the hydrolysis of glucose-6 phosphate (G6P) to glucose and inorganic phosphate. A deficiency in G6Pase activity causes type 1 glycogen storage disease (GSD-1), mainly characterised by hypoglycaemia. Genetic analyses of the two forms of this...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.09.022
更新日期:2012-03-01 00:00:00
abstract::The exosome is a complex of eleven subunits in yeast, involved in RNA processing and degradation. Despite the extensive in vivo functional studies of the exosome, little information is yet available on the structure of the complex and on the RNase and RNA binding activities of the individual subunits. The current mode...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.01.011
更新日期:2007-05-01 00:00:00
abstract::Human mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs), the enzymes which esterify tRNAs with the cognate specific amino acid, form mainly a different set of proteins than those involved in the cytosolic translation machinery. Many of the mt-aaRSs are of bacterial-type in regard of sequence and modular structural o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.02.005
更新日期:2009-05-01 00:00:00
abstract::Cell sterol supply is subjected to tight negative feedback regulation through the SREBP pathway. Upon cholesterol depletion, SREBP transcription factors become activated by cleavage of a membrane bound precursor form, which stimulates the expression of the genes encoding proteins of the cholesterol synthesis pathway. ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2006.09.015
更新日期:2007-02-01 00:00:00
abstract::The poisonous bite by Hippasa partita, a funnel web spider from the Indian subcontinent has been demonstrated to give rise to severe dermo- and myonecrosis. In this work a hemorrhagic metalloprotease, Partitagin was purified from H. partita venom by successive chromatography on Sephadex G-100, DEAE Sephadex A-50 and B...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.04.005
更新日期:2007-11-01 00:00:00
abstract::Over the years, imino proton exchange measurements have provided a description of base-pair opening and of properties of internal motions of nucleic acids such as the individual opening of base pairs and the insensitivity of base-pair lifetimes to the nature and stability of neighboring pairs. A recent determination o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)86936-2
更新日期:1997-12-01 00:00:00
abstract::The ability of asialo-thyroglobulin to bind the thyroid RHL-1 subunit of the asialoglycoprotein receptor has been investigated. Ligand blot assays show that the recombinant carbohydrate recognition domain of the thyroid RHL-1 subunit specifically interacts with rat desialated thyroglobulin. Moreover, RT-PCR and Wester...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80100-x
更新日期:1999-05-01 00:00:00
abstract::MicroRNAs (miRNAs) play profound roles in plant growth and development by regulating gene expression. Tetraploid plants often have better physical characteristics and stress tolerance than their diploid progenitors, but the role of miRNAs in this superiority is unclear. Paulownia tomentosa, (Paulowniaceae) is attracti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.02.008
更新日期:2014-07-01 00:00:00
abstract::Interferon-gamma is a key cytokine in tumor immunosurveillance. The recently described interferon-producing killer dendritic cell (IKDC), can be distinguished from other innate effectors by its ability to kill a large variety of tumor cells and to produce high amounts of interferon-gamma after encountering tumors in t...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.04.014
更新日期:2007-06-01 00:00:00
abstract::Although up to 25% of glucose released into circulation in the postabsorptive state comes from renal gluconeogenesis, the regulatory mechanisms of this process are still poorly recognized, comparing to hepatic ones. The aim of the present study was to examine if hypoxia-inducible factor-1 (HIF-1) might be involved in ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.02.002
更新日期:2020-01-01 00:00:00
abstract::The eosinophil cationic protein (ECP) is a human antimicrobial protein involved in the host immune defense that belongs to the pancreatic RNase A family. ECP displays a wide range of antipathogen activities. The protein is highly cationic and its bactericidal activity is dependant on both cationic and hydrophobic surf...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.10.005
更新日期:2011-02-01 00:00:00
abstract::Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.03.023
更新日期:2015-06-01 00:00:00
abstract::Split luciferase complementary assay has been used to investigate the effect of WD domain deletion on Apaf-1 oligomerization. Apaf-1 is an adaptor molecule in formation of apoptosome that activates caspase-9, an activation that is a key event in the mitochondrial cell death pathway. Structural studies suggest that nor...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.10.013
更新日期:2021-01-01 00:00:00
abstract::Glycogen synthase I was purified from bovine polymorphonuclear leucocytes (PMNs) by a procedure involving concanavalin A-Sepharose affinity chromatography. The purified glycogen-bound glycogen synthase I had a specific activity of 9.83 U/mg protein and the glycogen free enzyme 21 U/mg protein. Molecular ratio of the n...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80201-7
更新日期:1985-06-01 00:00:00
abstract::Snake venom phospholipases A2 (PLA2) share high sequence identities and a conserved structural scaffold, but show important functional differences. Only a few PLA2s have been purified and characterized from coral snake (Micrurus spp.) venoms, and their role in envenomation remains largely unknown. In this report, we d...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.03.008
更新日期:2017-06-01 00:00:00
abstract::Multiple forms of acetylcholinesterase have been studied in Goldfish adapted to 15 degrees C and 25 degrees C. Three forms of enzyme (sedimenting at 18S, 12S and 5S) could be distinguished by sucrose gradient centrifugation. Their molecular and enzymatic properties did not vary with environmental temperature. It was n...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80257-x
更新日期:1976-01-01 00:00:00
abstract::The elucidation of the multiple signaling cascades coupled to the TSH receptor has offered new approaches in the understanding of the pathogenesis of Graves' disease. Here we review findings showing that immunoglobulins from Graves' patients are heterogeneous, bind to different epitopes and, similarly to TSH, activate...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80090-x
更新日期:1999-05-01 00:00:00
abstract::We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of v...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.07.013
更新日期:2006-02-01 00:00:00
abstract::A lignin model compound, named in short guaiagylglycerol beta-guaiacyl ether (GGE), contains the beta-0-4 ether linkage that is common in the chemical structure of lignin. A Pseudomonas sp. (GU5) had been isolated as an organism able to grow with GGE as the sole source of carbon and energy. When grown on vanillate, th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80292-3
更新日期:1985-09-01 00:00:00
abstract::The ability to measure the thermodynamic stability of proteins with precision is important for both academic and applied research. Such measurements rely on mathematical models of the protein denaturation profile, i.e. the relation between a global protein signal, corresponding to the folding states in equilibrium, an...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.11.013
更新日期:2016-02-01 00:00:00