Abstract：
:The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI is the first structure of a member of Kunitz (STI) family formed by two noncovalently bound polypeptide chains and only one disulfide bridge. A standard Kunitz-type inhibitor has a single polypeptide chain and two disulfide bridges. Structural features granting CTI high inhibitory activity are discussed.
journal_name
Biochimiejournal_title
Biochimieauthors
Krauchenco S,Nagem RA,da Silva JA,Marangoni S,Polikarpov Idoi
10.1016/j.biochi.2004.03.004keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
167-72issue
3eissn
0300-9084issn
1638-6183pii
S0300908404000367journal_volume
86pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Split luciferase complementary assay has been used to investigate the effect of WD domain deletion on Apaf-1 oligomerization. Apaf-1 is an adaptor molecule in formation of apoptosome that activates caspase-9, an activation that is a key event in the mitochondrial cell death pathway. Structural studies suggest that nor...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.10.013
更新日期：2021-01-01 00:00:00
abstract：:When colonizing the digestive tract of mono-associated rats, Ruminococcus gnavus E1 - a bacterium isolated from human faeces - produced a trypsin-dependent anti-Clostridium perfringens substance collectively named Ruminococcin C (RumC). RumC was isolated from the caecal contents of E1-monocontaminated rats and found t...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.05.001
更新日期：2011-09-01 00:00:00
abstract：:The Rap family of small GTP-binding proteins is composed by four different members: Rap1A, Rap1B, Rap2A and Rap2B. In this work we report the identification and characterization of a fifth member of this family of small GTPases. This new protein is highly homologous to Rap2A and Rap2B, binds labeled GTP on nitrocellul...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.08.007
更新日期：2006-03-01 00:00:00
abstract：:Glucose-6 phosphatase (G6Pase), a key enzyme of glucose homeostasis, catalyses the hydrolysis of glucose-6 phosphate (G6P) to glucose and inorganic phosphate. A deficiency in G6Pase activity causes type 1 glycogen storage disease (GSD-1), mainly characterised by hypoglycaemia. Genetic analyses of the two forms of this...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.09.022
更新日期：2012-03-01 00:00:00
abstract：:Adipose tissue lipoprotein lipase activity (LPL) falls dramatically during experimental infection of mice by Plasmodium chabaudi. The decrease in this activity accounts for the seric accumulation of triacyl-glyceride-rich lipoproteins (chylomicrons mainly and Very Low Density Lipoproteins (VLDL) partly). This loss of ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(82)80414-8
更新日期：1982-02-01 00:00:00
abstract：:Human mitochondrial diseases, defined as the diseases due to a mitochondrial oxidative phosphorylation defect, represent a large group of very diverse diseases with respect to phenotype and genetic causes. They present with many unsolved issues, the comprehensive analysis of which is beyond the scope of this review. W...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2013.08.012
更新日期：2014-05-01 00:00:00
abstract：:Translational control is a common mechanism used to regulate gene expression and occur in bacteria to mammals. Typically in translational control, an RNA-binding protein binds to a unique sequence in the mRNA to regulate protein synthesis by the ribosomes. Alternatively, a protein may bind to or modify a translation f...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.02.005
更新日期：2015-07-01 00:00:00
abstract：:1. Ghe kinetic and binding parameters of adenine-nucleotide transport have been studied in mitochondria isolated from yeast cells in which the mitochondrial protein-synthetizing system had been inhibited by growth in the presence of erythromycin. These parameters have also been studied in promitochondria isolated from...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80120-4
更新日期：1976-01-01 00:00:00
abstract：:In the bacterium Escherichia coli, H-NS-(H1, H1a) is a heat-stable protein with a molecular mass of 15.5 kDa involved in nucleoid organisation and gene regulation linked to certain signal transduction pathways. We have shown that, following addition of preparations of everted inner membrane vesicles, heat-stable cleav...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)80025-9
更新日期：1997-06-01 00:00:00
abstract：:Lipid membranes have an essential role in most physiological processes including cell protection, cell-to-cell communication and regulation of intracellular signaling. These multiple roles of biological membranes prompted the study and the development of artificial lipid membranes with the primary aim to reconstitute ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2018.11.011
更新日期：2019-02-01 00:00:00
abstract：:The aim of the present work was to establish the thermodynamic and functional differences in the protein-protein interactions between the components of the P450-dependent mitochondrial (mit) and microsomal (mic) monooxygenase systems using 12 different isoforms of cytochromes P450 and two redox partners, NADPH-depende...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.04.020
更新日期：2019-07-01 00:00:00
abstract：:Human prophospholipase A2 was purified from pancreatic juice. The protein has a molecular weight of 14500 and a free N-terminal residue identified as aspartic acid (or asparagine). The amino acid composition was determined. Partial immunological identity has been obtained between human and porcine prophospholipase A2....
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(81)80216-7
更新日期：1981-08-01 00:00:00
abstract：:Latices from Asclepias spp are used in wound healing and the treatment of some digestive disorders. These pharmacological actions have been attributed to the presence of cysteine proteases in these milky latices. Asclepias curassavica (Asclepiadaceae), "scarlet milkweed" is a perennial subshrub native to South America...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.07.017
更新日期：2009-11-01 00:00:00
abstract：:The very end of the chromosome is called the telomere and is composed of DNA repeat sequences and associated proteins. Genetic and biochemical analyses of this complex, the telosome, lead to the hypothesis that transcription and DNA replication are submitted to position effects mediated by the telomere proximity. Telo...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(96)88201-0
更新日期：1995-01-01 00:00:00
abstract：:The class I glutamine (Gln) tRNA synthetase interacts with the anticodon and acceptor stem of glutamine tRNA. RNA hairpin helices were designed to probe acceptor stem and anticodon stem-loop contacts. A seven-base pair RNA microhelix derived from the acceptor stem of tRNA(Gln) was aminoacylated by Gln tRNA synthetase....
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90003-b
更新日期：1993-01-01 00:00:00
abstract：:We report Raman spectra of various cholinesterases: lytic tetrameric forms (G4) obtained by tryptic digestion of asymmetric acetylcholinesterase (AChE) from Torpedo californica and Electrophorus electricus, a PI-PLC-treated dimeric form (G2) of AChE from T marmorata, and the soluble tetrameric form (G4) of butyrylchol...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90167-y
更新日期：1991-11-01 00:00:00
abstract：:Protein 3D structures support their biological functions. As the number of protein structures is negligible in regards to the number of available protein sequences, prediction methodologies relying only on protein sequences are essential tools. In this field, protein secondary structure prediction (PSSPs) is a mature ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.09.006
更新日期：2020-12-01 00:00:00
abstract：:Changes in the cytosolic Ca(2+) concentration ([Ca(2+)](c)) translate a variety of extracellular signals into widely diverse intracellular effects, ranging from secretion to movement, proliferation and also cell death. As regards the last one, it has long been known that large [Ca(2+)](c) increases lead cells to death...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(02)01373-1
更新日期：2002-02-01 00:00:00
abstract：:We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of v...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.07.013
更新日期：2006-02-01 00:00:00
abstract：:Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.03.023
更新日期：2015-06-01 00:00:00
abstract：:Bacterial type II fatty acid synthesis (FASII) is a target for novel antibiotic development. All bacteria encode for mechanisms to incorporate exogenous fatty acids, and some bacteria can use exogenous fatty acids to bypass FASII inhibition. Bacteria encode three different mechanisms for activating exogenous fatty aci...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2017.06.015
更新日期：2017-10-01 00:00:00
abstract：:We describe PGtrans, a new and freely available protein sequence databank (2625 sequences, 554198 amino-acids). This data bank is routinely produced by automatic computer translation of the nucleotide sequence library GenBank. The information needed for the translation process (transcriptional orientation, location of...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80261-3
更新日期：1985-05-01 00:00:00
abstract：:Epigenetic alterations are involved in every step of carcinogenesis. The development of chromatin-modifying agents (CMAs) has provided the ability to fight cancer by reversing these alterations. Currently, four CMAs have been approved for cancer treatment; two DNA demethylating agents and two deacetylase inhibitors. A...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.05.012
更新日期：2012-11-01 00:00:00
abstract：:Chronic hypobaric hypoxia induced muscle atrophy results in decreased physical performance at high altitude. Curcumin has been shown to have muscle sparing effects under cachectic conditions. However, the protective effects of curcumin under chronic hypobaric hypoxia have not been studied till now. Therefore, the pres...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.10.012
更新日期：2019-01-01 00:00:00
abstract：:A major tolerance mechanism that functions to replicate damaged genomic DNA across lesions that have escaped elimination by repair mechanism is translesion DNA synthesis (TLS). DNA polymerase kappa (Pol kappa), a specialised low-fidelity DNA polymerase which is able to perform DNA synthesis across several damaged base...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2005.02.007
更新日期：2005-07-01 00:00:00
abstract：:Nitroxyl (HNO) is a molecule of significant interest due to its unique pharmacological properties, particularly within the cardiovascular system. A large portion of HNO biological effects can be attributed to its reactivity with protein thiols, where it can generate disulfide bonds. Evidence from studies in erythrocyt...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.12.005
更新日期：2012-03-01 00:00:00
abstract：:This review article aims to gather all the knowledge on two important proteins associated with Hevea brasiliensis rubber particles: namely the rubber elongation factor (REF) and the small rubber particle protein (SRPP). It covers more then three decades of research on these two proteins and their homologues in plants,...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2014.07.002
更新日期：2014-11-01 00:00:00
abstract：:Multiple forms of acetylcholinesterase have been studied in Goldfish adapted to 15 degrees C and 25 degrees C. Three forms of enzyme (sedimenting at 18S, 12S and 5S) could be distinguished by sucrose gradient centrifugation. Their molecular and enzymatic properties did not vary with environmental temperature. It was n...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80257-x
更新日期：1976-01-01 00:00:00
abstract：:Three mutanase (alpha-1,3-glucanase)-producing microorganisms isolated from soil samples were identified as a relatives of Paenibacillus. A mutanase was purified to homogeneity from cultures of each, and the molecular masses of the purified enzymes were approximately 132, 141, and 141kDa, respectively. The correspondi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.09.018
更新日期：2008-03-01 00:00:00
abstract：:Mammalian chymotrypsin-like serine proteases (SPs) are one of the best-studied family of enzymes with roles in a wide range of physiological processes, including digestion, blood coagulation, fibrinolysis and humoral immunity. Extracellular SPs can form cascades, in which one protease activates the zymogen of the next...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.10.007
更新日期：2016-03-01 00:00:00
