Acceptor stem and anticodon RNA hairpin helix interactions with glutamine tRNA synthetase.

abstract：:Two methods were used to estimate the intracellular phospholipase activity in rat heart: one using exogenous radioactive substrate dispersed as unilamellar vesicles; the other using endogenous membrane hydrolysis and subsequent phospholipid and lysophospholipid separation by high-performance liquid chromatography and ...

journal_title：Biochimie

authors： Termine E,Léonardi J,Lafont H,Nalbone G

更新日期：1987-03-01 00:00:00

abstract：:A synthetic phosphonate inhibitor designed for lipase inhibition but displaying a broader range of activity was covalently immobilized on a solid support to generate a function-directed tool targeting serine hydrolases. To achieve this goal, straightforward and reliable analytical techniques were developed, allowing t...

journal_title：Biochimie

authors： Delorme V,Raux B,Puppo R,Leclaire J,Cavalier JF,Marc S,Kamarajugadda PK,Buono G,Fotiadu F,Canaan S,Carrière F

更新日期：2014-12-01 00:00:00

abstract：:While many works analyse lipid composition of cellular nuclei, essentially of liver, few describe bird erythrocyte nuclei. For them very discordant results are reported due to very different methods of preparation. In the method we adopted a good reproducibility is controlled by electron microscopy, evaluation of spec...

journal_title：Biochimie

authors： Souillard C,Soula G

更新日期：1976-01-01 00:00:00

abstract：:Oxidative stress, characterized by the accumulation of reactive oxygen species (ROS), is known to have numerous detrimental effects on the myocardium such as the induction of apoptotic cell death, hypertrophy, fibrosis, dysfunction, and dilatation. Over the past several years, we have shown that mitochondrial NADP(+)-...

journal_title：Biochimie

authors： Lee JH,Park JW

更新日期：2014-04-01 00:00:00

abstract：:alpha-Latrotoxin, a protein toxin present in the venom of black widow spider, interacts with membrane receptors of neurons and other secretory cells to stimulate exocytosis. Two types of receptors have been identified and cloned. Our attention has been focused on the calcium independent receptor, a G-protein coupled r...

journal_title：Biochimie

authors： Pescatori M,Grasso A

更新日期：2000-09-01 00:00:00

abstract：:Stroke is a worldwide major cause of mortality and morbidity. Preclinical studies have identified over 1000 molecules with brain-protective properties. More than 200 clinical trials have evaluated neuroprotective candidates for ischemic stroke yet, to date almost all failed, leading to a re-analysis of treatment strat...

journal_title：Biochimie

authors： Blondeau N

更新日期：2016-01-01 00:00:00

abstract：:The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence propert...

journal_title：Biochimie

authors： Klein R,Tatischeff I,Tham G,Grolière CA

更新日期：1991-10-01 00:00:00

abstract：:Acetylcholinesterase (AChE) has been identified and purified from the venom of desert cobra (W aegyptia) to apparent homogeneity using a TSK G 3000 SW gel filtration column and a Mono Q anion-exchange column. AChE was purified to homogeneity as established by sodium dodecylsulfate/polyacrylamide gel electrophoresis. T...

journal_title：Biochimie

authors： Duhaiman AS,Alhomida AS,Rabbani N,Kamal MA,al-Jafari AA

更新日期：1996-01-01 00:00:00

abstract：:Since discovery of high-temperature superconductive (HTS) ceramics by Bednorz and Muller in 1986, there has been an accelerated development of cold technologies in industry, including the domain of NMR detection. The purpose of this paper is to fix ideas about the stage that cryogenic radio frequency (RF) probe techni...

journal_title：Biochimie

authors： Darrasse L,Ginefri JC

更新日期：2003-09-01 00:00:00

abstract：:The mouse interferon alpha 7 gene, the signal sequence of which has been removed by oligonucleotide-directed mutagenesis, was introduced into a Bacillus subtilis secretion vector containing the promoter and the signal sequence of the B. subtilis levansucrase gene. Different B. subtilis strains were transformed with th...

journal_title：Biochimie

authors： Dion M,Rapoport G,Doly J

更新日期：1989-06-01 00:00:00

abstract：:Triiodothyronine (T3), injected daily into rats, induces heart hypertrophy. In a recent work we have shown that a single T3 injection stimulates RNA synthesis and modifies the translational products of myocardial mRNAs in reticulocyte lysates, quantitatively and qualitatively. In this work we show that T3 induces smal...

journal_title：Biochimie

authors： Raymondjean M,Leibovitch SA,Leibovitch MP,Harel J,Kruh J

更新日期：1984-09-01 00:00:00

abstract：:Kinetic properties of PGM1 and PGM2 phosphoglucomutase "primary" isoenzymes from human erythrocytes were studied. The two enzyme forms share a "ping-pong" kinetic mechanism and show similar Km for substrate (glucose 1-P) and cofactor (glucose 1,6-P2). Micromolar concentrations of fructose 1,6-P2 and glycerate 2,3-P2 i...

journal_title：Biochimie

authors： Ninfali P,Accorsi A,Palma F,Fazi A,Piatti E,Chiarantini L,Fornaini G

更新日期：1984-09-01 00:00:00

abstract：:Using a new extended set of 13 amber suppressors in E coli, systematic amino-acid replacements were performed at positions 104(E) and 238(G) of TEM-1 beta-lactamase from PUC19. The enzyme is tolerant to most substitutions tested at position 104. Missense revertants E104K, E104S or E104Y exhibited only minor changes in...

journal_title：Biochimie

authors： Lenfant F,Labia R,Masson JM

更新日期：1990-06-01 00:00:00

abstract：:Ligation of CD47 by its natural ligand thrombospondin (TSP), or cross-linking by CD47 antibodies, triggers caspase-independent cell death in normal and leukemic cells. This kind of cell death is characterised by the cytoplasmic events of apoptosis including externalisation of phosphatidylserines and mitochondria swell...

journal_title：Biochimie

authors： Roué G,Bitton N,Yuste VJ,Montange T,Rubio M,Dessauge F,Delettre C,Merle-Béral H,Sarfati M,Susin SA

更新日期：2003-08-01 00:00:00

abstract：:Theoretic and experimental arguments are surveyed which justify the setting up, within the family of pyridoxal-P-dependent lyases, of a special subgroup that comprizes several enzymes catalyzing exclusively beta-replacement reactions of alpha-aminoacids with electronegative substituents in the beta-position. The autho...

journal_title：Biochimie

authors： Braunstein AE,Goryachenkova EV

更新日期：1976-01-01 00:00:00

abstract：:The Nicotiana tabacum Nt-4/1 protein is a plant-specific protein of unknown function. Analysis of bacterially expressed Nt-4/1 protein in vitro revealed that the protein secondary structure is mostly alpha-helical and suggested that it could consist of three structural domains. Earlier studies of At-4/1, the Arabidops...

journal_title：Biochimie

authors： Solovyev AG,Minina EA,Makarova SS,Erokhina TN,Makarov VV,Kaplan IB,Kopertekh L,Schiemann J,Richert-Pöggeler KR,Morozov SY

更新日期：2013-07-01 00:00:00

abstract：:In this review, we show how the stability of the asymmetric transverse distribution of phospholipids and the physiological role of the asymmetric distribution can be explained. Experiments with paramagnetic or fluorescent lipids enabled us to show that in fresh red blood cells, i.e. containing ATP, and in resealed gho...

journal_title：Biochimie

authors： Devaux PF,Zachowski A,Favre E,Fellmann P,Cribier S,Geldwerth D,Hervé P,Seigneuret M

更新日期：1986-03-01 00:00:00

abstract：:The effects on intra- and extracellular pH of two polyenic derivatives of amphotericin B, N-fructosyl amphotericin B and N-fructosyl amphotericin B methyl-ester, were tested on HL-60 promyelocytic leukemia cells. Both derivatives raised the internal pH and reduced the external pH in weakly buffered medium. These resul...

journal_title：Biochimie

authors： Charretier E,Wietzerbin J

更新日期：1989-01-01 00:00:00

abstract：:The widespread nature of protein phosphorylation/dephosphorylation underscores its key role in cell signaling metabolism, growth and differentiation. Tyrosine phosphorylation of cytoplasmic proteins is a critical event in the regulation of intracellular signaling pathways activated by external stimuli. An adequate bal...

journal_title：Biochimie

authors： Zambuzzi WF,Milani R,Teti A

更新日期：2010-04-01 00:00:00

abstract：:A novel bgl1 gene, encoding GH3 family β-glucosidase from Penicillium verruculosum (PvBGL), was cloned and heterologously expressed in P. canescens RN3-11-7 (niaD-) strain under the control of the strong xylA gene promoter. The recombinant rPvBGL was purified and their properties were studied in comparison with those ...

journal_title：Biochimie

authors： Volkov PV,Rozhkova AM,Zorov IN,Sinitsyn AP

更新日期：2020-01-01 00:00:00

abstract：:Chronic hypobaric hypoxia induced muscle atrophy results in decreased physical performance at high altitude. Curcumin has been shown to have muscle sparing effects under cachectic conditions. However, the protective effects of curcumin under chronic hypobaric hypoxia have not been studied till now. Therefore, the pres...

journal_title：Biochimie

authors： Chaudhary P,Sharma YK,Sharma S,Singh SN,Suryakumar G

更新日期：2019-01-01 00:00:00

abstract：:Aptamers are short single-stranded nucleic acid molecules that are selected in vitro from a large random sequence library based on their high and specific affinity to a target molecule by a process known as SELEX. Cell-SELEX that employs whole living cells overexpressing the defined cell surface proteins (for selectio...

journal_title：Biochimie

authors： Takahashi M,Sakota E,Nakamura Y

更新日期：2016-12-01 00:00:00

abstract：:We report Raman spectra of various cholinesterases: lytic tetrameric forms (G4) obtained by tryptic digestion of asymmetric acetylcholinesterase (AChE) from Torpedo californica and Electrophorus electricus, a PI-PLC-treated dimeric form (G2) of AChE from T marmorata, and the soluble tetrameric form (G4) of butyrylchol...

journal_title：Biochimie

authors： Aslanian D,Grof P,Bon S,Masson P,Négrerie M,Chatel JM,Balkanski M,Taylor P,Massoulié J

更新日期：1991-11-01 00:00:00

abstract：:Bacterial superantigens (SAg) are the most potent activators of human T lymphocytes and recombinant immunotoxin using bacterial SAg shows promising clinical values. To engineer superantigen for immunotherapy of hepatocellular carcinoma, we genetically fused the superantigen staphylococcus enterotoxin A (SEA(D(227)A)) ...

journal_title：Biochimie

authors： Hao HJ,Jiang YQ,Zheng YL,Ma R,Yu DW

更新日期：2005-08-01 00:00:00

abstract：:Canine S100A12 (cS100A12) is a calcium-binding protein of the S100 superfamily of EF-hand proteins, and its expression is restricted to neutrophils and monocytes. Interaction of S100A12 with the receptor for advanced glycation end products (RAGE) has been suggested to play a central role in inflammation. Moreover, S10...

journal_title：Biochimie

authors： Heilmann RM,Suchodolski JS,Steiner JM

更新日期：2010-12-01 00:00:00

abstract：:This paper deals with the discovery of plasmalogen deficiency in the cerebro-hepato-renal (Zellweger) syndrome and discusses how this has led to the development of postnatal and prenatal diagnostic procedures for this and a number of related peroxisomal disorders in man that show a general impairment in the biosynthes...

journal_title：Biochimie

authors： van den Bosch H,Schrakamp G,Hardeman D,Zomer AW,Wanders RJ,Schutgens RB

更新日期：1993-01-01 00:00:00

abstract：:Porcine pancreatic extracts (PPE), also named pancreatin, are commonly used as a global source of pancreatic enzymes for enzyme replacement therapy in patients with exocrine pancreatic insufficiency. They are considered as a good substitute of human pancreatic enzymes and they have become a material of choice for in v...

journal_title：Biochimie

authors： Salhi A,Amara S,Mansuelle P,Puppo R,Lebrun R,Gontero B,Aloulou A,Carrière F

更新日期：2020-02-01 00:00:00

abstract：:The number of proteins known to contain collagen-like triple helical domains is rapidly increasing. The functions of these domains are to provide molecular rods that separate spatially non-triple helical domains with varied properties and structures and to permit lateral interactions between molecules. Two-thirds of t...

journal_title：Biochimie

authors： van der Rest M,Garrone R

更新日期：1990-06-01 00:00:00

abstract：:Adrenoleukodystrophy (ALD) is an X-linked peroxisomal disorder characterized by a progressive demyelination of the central nervous system and adrenal insufficiency. Clinical phenotypes of different severity are frequently observed within the same kindred. ALD is characterized biochemically by the accumulation of very-...

journal_title：Biochimie

authors： Aubourg P,Mosser J,Douar AM,Sarde CO,Lopez J,Mandel JL

更新日期：1993-01-01 00:00:00

abstract：:Bacterial type II fatty acid synthesis (FASII) is a target for novel antibiotic development. All bacteria encode for mechanisms to incorporate exogenous fatty acids, and some bacteria can use exogenous fatty acids to bypass FASII inhibition. Bacteria encode three different mechanisms for activating exogenous fatty aci...

journal_title：Biochimie

authors： Yao J,Rock CO

更新日期：2017-10-01 00:00:00