Abstract：
:Two methods were used to estimate the intracellular phospholipase activity in rat heart: one using exogenous radioactive substrate dispersed as unilamellar vesicles; the other using endogenous membrane hydrolysis and subsequent phospholipid and lysophospholipid separation by high-performance liquid chromatography and quantification by phosphorus determination. We found that the endogenous method provided a higher hydrolysis rate than the exogenous method and that phosphatidyl ethanolamine was a better substrate than phosphatidyl choline.
journal_name
Biochimiejournal_title
Biochimieauthors
Termine E,Léonardi J,Lafont H,Nalbone Gdoi
10.1016/0300-9084(87)90049-6subject
Has Abstractpub_date
1987-03-01 00:00:00pages
245-8issue
3eissn
0300-9084issn
1638-6183pii
0300-9084(87)90049-6journal_volume
69pub_type
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