Cloning, purification and study of recombinant GH3 family β-glucosidase from Penicillium verruculosum.

abstract：:Fluorescence techniques were used to detect changes in the conformation of tRNA(Phe) that may occur during the peptidyl transferase reaction in which the tRNA appears to move between binding sites on ribosomes. Such a conformational change may be a fundamental part of the translocation mechanism by which tRNA and mRNA...

journal_title：Biochimie

authors： Odom OW,Hardesty B

更新日期：1987-09-01 00:00:00

abstract：:Betaine aldehyde dehydrogenase from the human opportunistic pathogen Pseudomonas aeruginosa (PaBADH) catalyzes the irreversible, NAD(P)(+)-dependent oxidation of betaine aldehyde, producing glycine betaine, an osmoprotectant. PaBADH participates in the catabolism of choline and likely in the defense against the osmoti...

journal_title：Biochimie

authors： Zaldívar-Machorro VJ,López-Ortiz M,Demare P,Regla I,Muñoz-Clares RA

更新日期：2011-02-01 00:00:00

abstract：:Wheat germ lysate was used as a model system for in vitro translation. We show that an increase of the exchange surface between the reaction mixture and the atmosphere enhanced the amount of incorporated cysteine, indicating that early arrest of protein synthesis previously observed in such a system was due to oxygen ...

journal_title：Biochimie

authors： Luciani N,Hess K,Belleville F,Nabet P

更新日期：1995-01-01 00:00:00

abstract：:The suhB gene of Escherichia coli has been defined by its mutant allele that suppresses other mutants in secY, rpoH, dnaB, and era. The suhB mutant by itself is cold sensitive, and is shown to have defects in protein synthesis. Starting with the suhB cold-sensitive mutant, cold-resistant suppressors were isolated. The...

journal_title：Biochimie

authors： Inada T,Nakamura Y

更新日期：1995-01-01 00:00:00

abstract：:Ciguatoxin-like substances were extracted from the viscera or the flesh of eight Caribbean fish species, including small invertebrate feeders and large carnivores. The had similar properties, i.e. pharmacological action, solubility, chromatographic behaviour on silicic acid or Sephadex LH 20 column, stability in a wea...

journal_title：Biochimie

authors： Vernoux JP,Abbad el Andaloussi S

更新日期：1986-03-01 00:00:00

abstract：:P53 is a transcription factor also called the "cellular gatekeeper of genome" because it can induce cell cycle arrest in G1, apoptosis or affect DNA replication in response to DNA damage. Wild type p53 is localised in both the cytoplasm and nucleus of first trimester trophoblastic cells (CTB). Immunoblotting of CTB wi...

journal_title：Biochimie

authors： Cohen M,Wuillemin C,Bischof P

更新日期：2008-06-01 00:00:00

abstract：:The translation factor EF-P is required for efficient prokaryotic peptide bond synthesis on 70S ribosomes from fMet-tRNAfMet. This protein has been purified from Escherichia coli cells and the gene, efp, encoding it has been cloned and sequenced. We have isolated recombinant clones which overexpress a protein that co-...

journal_title：Biochimie

authors： Aoki H,Adams SL,Turner MA,Ganoza MC

更新日期：1997-01-01 00:00:00

abstract：:Unspliced human immunodeficiency virus-1 (HIV-1) mRNA is capped and therefore can be translated via conventional scanning mechanism. In addition, its 5' untranslated region (5'UTR) is thought to function as an internal ribosome entry site (IRES) during G2/M-phase of cell cycle or when cap-dependent translation is inhi...

journal_title：Biochimie

authors： Smirnova VV,Terenin IM,Khutornenko AA,Andreev DE,Dmitriev SE,Shatsky IN

更新日期：2016-02-01 00:00:00

abstract：:Cotranslational translocation of exportable proteins across the RER membrane prior to their release into the extracellular space has been essentially described by use of canine pancreatic microsomal membranes. Intracisternal segregation of nascent secretory proteins was observed to be irreversible and proteolytic remo...

journal_title：Biochimie

authors： Scheele GA

更新日期：1988-09-01 00:00:00

abstract：:Classic studies suggested that the common mammalian sialic acid N-glycolylneuraminic acid (Neu5Gc) is an oncofetal antigen in humans, being immunogenic in adult humans and yet apparently expressed in human fetuses and tumors. We and others have recently found that the human deficiency of Neu5Gc can be explained by an ...

journal_title：Biochimie

authors： Varki A

更新日期：2001-07-01 00:00:00

abstract：:The family of interferon regulatory transcription factors (IRF) participates in the virus-induced and dsRNA-stimulated transcriptional regulation of either type I IFN genes or a definite set of genes which can also be activated by IFN. In this review, we place emphasis on the role of IRF-3 that associates with the coa...

journal_title：Biochimie

authors： Bragança J,Civas A

更新日期：1998-08-01 00:00:00

abstract：:Low bone formation in osteoporosis is associated with a shift from osteoblastic to adipogenic differentiation of mesenchymal stem cells (MSC) inducing a concomitant lipotoxic milieu within the bone marrow. Strontium ranelate (SrRN), a treatment for osteoporosis, has both anti-resorptive and anabolic effects on bone. T...

journal_title：Biochimie

authors： Vidal C,Gunaratnam K,Tong J,Duque G

更新日期：2013-04-01 00:00:00

abstract：:Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC ...

journal_title：Biochimie

authors： Bruneau G,Krieger-Poullet M,Coge F,Borri-Voltattorni C,Gros F,Thibault J

更新日期：1990-01-01 00:00:00

abstract：:Carbonic anhydrases (CAs, EC 4.2.1.1) are metalloenzymes found throughout the phylogenetic tree. The β-class carbonic anhydrases (β-CAs) are the predominating class of CAs in plants. Growing evidence underscores the importance of β-CAs in plant immunity and environmental adaptation in addition to their roles in photos...

journal_title：Biochimie

authors： Wang M,Zhang Q,Liu FC,Xie WF,Wang GD,Wang J,Gao QH,Duan K

更新日期：2014-02-01 00:00:00

abstract：:Interferon-gamma is a key cytokine in tumor immunosurveillance. The recently described interferon-producing killer dendritic cell (IKDC), can be distinguished from other innate effectors by its ability to kill a large variety of tumor cells and to produce high amounts of interferon-gamma after encountering tumors in t...

journal_title：Biochimie

authors： Bonmort M,Ullrich E,Mignot G,Jacobs B,Chaput N,Zitvogel L

更新日期：2007-06-01 00:00:00

abstract：:A set of sequences can be defined by their common subsequences, and the length of these is a measure of the overall resemblance of the set. Each subsequence corresponds to a succession of symbols embedded in every sequence, following the same order but not necessarily contiguous. Determining the longest common subsequ...

journal_title：Biochimie

authors： Saurin W,Marlière P

更新日期：1985-05-01 00:00:00

abstract：:About 150 participants attended the symposium organised at the Palais de la Bourse in Bordeaux, France on September 22-23, 2017. Thirty speakers from all over the world delivered lectures covering selection processes, aptamer chemistry and innovative applications of these powerful tools that display major advantages o...

journal_title：Biochimie

authors： Toulmé JJ,Azéma L,Darfeuille F,Dausse E,Durand G,Paurelle O

更新日期：2018-02-01 00:00:00

abstract：:Since 2012, the interest for TMEM165 increased due to its implication in a rare genetic human disease named TMEM165-CDG (Congenital Disorder(s) of Glycosylation). TMEM165 is a Golgi localized protein, highly conserved through evolution and belonging to the uncharacterized protein family 0016 (UPF0016). Although the pr...

journal_title：Biochimie

authors： Lebredonchel E,Houdou M,Potelle S,de Bettignies G,Schulz C,Krzewinski Recchi MA,Lupashin V,Legrand D,Klein A,Foulquier F

更新日期：2019-10-01 00:00:00

abstract：:Lipolytic activities of Yarrowia lipolytica LIP2 lipase (YLLIP2), human pancreatic (HPL) and dog gastric (DGL) lipases were first compared using lecithin-stabilized triacylglycerol (TAG) emulsions (Intralipid) at various pH and bile salt concentrations. Like DGL, YLLIP2 was able to hydrolyze TAG droplets covered by a ...

journal_title：Biochimie

authors： Bénarouche A,Point V,Carrière F,Cavalier JF

更新日期：2014-07-01 00:00:00

abstract：:The kinetoplastid protozoan Leishmania encodes major surface glycoprotein GP63, a zinc metallo-peptidase (EC.3.4.24.36) expressed both in promastigote and amastigote life stages. In the present study, we explored for the first time the role of C-terminal domain (CTD) in proteinase activity by serial truncation of Leis...

journal_title：Biochimie

authors： Mazumder S,Ganguly A,Ali N

更新日期：2010-12-01 00:00:00

abstract：:In the recent years, we have provided evidence that Tumor Protein 53-Induced Nuclear Protein 1 (TP53INP1) is a key stress protein with antioxidant-associated tumor suppressive function. The TP53INP1 gene, which is highly conserved in mammals, is over-expressed during stress responses including inflammation. This gene ...

journal_title：Biochimie

authors： Saadi H,Seillier M,Carrier A

更新日期：2015-11-01 00:00:00

abstract：:Over the past 200 years emissions of toxic heavy metals have risen tremendously and significantly exceed those from natural sources for practically all metals. Uptake and accumulation by crop plants represents the main entry pathway for potentially health-threatening toxic metals into human and animal food. Of major c...

journal_title：Biochimie

authors： Clemens S

更新日期：2006-11-01 00:00:00

abstract：:We have recently reported that α(2)β(1) and α(1)β(1) isozymes of Na(+)/K(+)-ATPase (NKA) are localized in the caveolae whereas only the α(1)β(1) isozyme of NKA is localized in the non-caveolae fraction of pulmonary artery smooth muscle cell membrane. It is well known that different isoforms of NKA are regulated differ...

journal_title：Biochimie

authors： Dey K,Roy S,Ghosh B,Chakraborti S

更新日期：2012-04-01 00:00:00

abstract：:Long-term treatment with ambroxol (ABX), a bronchial expectorant, was found to prevent acute exacerbation of chronic obstructive pulmonary disease (AECOPD). The underlying mechanism remains unclear. To address this, we have investigated the effect of ABX on critical antioxidant proteins thioredoxin (Trx) and thioredox...

journal_title：Biochimie

authors： Huang J,Xu J,Tian L,Zhong L

更新日期：2014-02-01 00:00:00

abstract：:Present knowledge of the different enzymatic steps of the denitrification chains in various bacteria, particularly Paracoccus denitrificans and Pseudomonas aeruginosa has been briefly reviewed. The question whether nitric oxide (NO), nitrous oxide (N2O) and other nitrogen derivatives are obligatory intermediates has b...

journal_title：Biochimie

authors： Henry Y,Bessières P

更新日期：1984-04-01 00:00:00

abstract：:Leptin is a cytokine secreted by the adipose tissue that is involved in the control of body weight. We previously showed that a point mutation (R105W) in leptin results in leptin deficiency, marked obesity and hypogonadism in humans adults. Expression in COS1 cells showed impaired secretion and intracellular accumulat...

journal_title：Biochimie

authors： Boute N,Zilberfarb V,Camoin L,Bonnafous S,Le Marchand-Brustel Y,Issad T

更新日期：2004-06-01 00:00:00

abstract：:Inhibition of polypeptide chain elongation with the mRNA-complementary (antisense) oligonucleotide has been realized through a RNase H independent mechanism. Nuclease resistant complementary non-natural alpha-17-mer oligonucleotide did not inhibit cell-free protein biosynthesis of beta-globin in the wheat germ system ...

journal_title：Biochimie

authors： Boutorine AS,Boiziau C,Le Doan T,Toulmé JJ,Hélène C

更新日期：1992-05-01 00:00:00

abstract：:Recent work has shown that a DBF4 analog in yeast may be a target of nitrogen-containing bisphosphonates. DBF4 is an essential protein kinase required for DNA replication from primary eukaryotes to humans and appears to play a critical role in the S-phase checkpoint. It is also required for cell migration and cell sur...

journal_title：Biochimie

authors： Mansouri M,Mirzaei SA,Lage H,Mousavi SS,Elahian F

更新日期：2014-04-01 00:00:00

abstract：:Human mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs), the enzymes which esterify tRNAs with the cognate specific amino acid, form mainly a different set of proteins than those involved in the cytosolic translation machinery. Many of the mt-aaRSs are of bacterial-type in regard of sequence and modular structural o...

journal_title：Biochimie

authors： Messmer M,Blais SP,Balg C,Chênevert R,Grenier L,Lagüe P,Sauter C,Sissler M,Giegé R,Lapointe J,Florentz C

更新日期：2009-05-01 00:00:00

abstract：:(-)-Epigallocatechin-3-gallate (EGCG), a well-known chemopreventive factor, induces cancer cells undergoing apoptosis. Over the last several years, we have shown that the mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) functions as an antioxidant and anti-apoptotic protein by supplying NADPH to antioxi...

journal_title：Biochimie

authors： Kil IS,Jung KH,Nam WS,Park JW

更新日期：2011-10-01 00:00:00