Abstract：
:Bacterial type II fatty acid synthesis (FASII) is a target for novel antibiotic development. All bacteria encode for mechanisms to incorporate exogenous fatty acids, and some bacteria can use exogenous fatty acids to bypass FASII inhibition. Bacteria encode three different mechanisms for activating exogenous fatty acids for incorporation into phospholipid synthesis. Exogenous fatty acids are converted into acyl-CoA in Gammaproteobacteria such as E. coli. Acyl-CoA molecules constitute a separate pool from endogenously synthesized acyl-ACP. Acyl-CoA can be used for phospholipid synthesis or broken down by β-oxidation, but cannot be used for lipopolysaccharide synthesis. Exogenous fatty acids are converted into acyl-ACP in some Gram-negative bacteria. The resulting acyl-ACP undergoes the same fates as endogenously synthesized acyl-ACP. Exogenous fatty acids are converted into acyl-phosphates in Gram-positive bacteria, and can be used for phospholipid synthesis or become acyl-ACP. Only the order Lactobacillales can use exogenous fatty acids to bypass FASII inhibition. FASII shuts down completely in presence of exogenous fatty acids in Lactobacillales, allowing Lactobacillales to synthesize phospholipids entirely from exogenous fatty acids. Inhibition of FASII cannot be bypassed in other bacteria because FASII is only partially down-regulated in presence of exogenous fatty acid or FASII is required to synthesize essential metabolites such as β-hydroxyacyl-ACP. Certain selective pressures such as FASII inhibition or growth in biofilms can select for naturally occurring one step mutations that attenuate endogenous fatty acid synthesis. Although attempts have been made to estimate the natural prevalence of these mutants, culture-independent metagenomic methods would provide a better estimate.
journal_name
Biochimiejournal_title
Biochimieauthors
Yao J,Rock COdoi
10.1016/j.biochi.2017.06.015subject
Has Abstractpub_date
2017-10-01 00:00:00pages
30-39eissn
0300-9084issn
1638-6183pii
S0300-9084(17)30170-0journal_volume
141pub_type
杂志文章,评审相关文献
BIOCHIMIE文献大全abstract：:Plant sucrose transporters (SUTs) are functional as sucrose-proton-cotransporters with an optimal transport activity in the acidic pH range. Recently, the pH optimum of the Solanum tuberosum sucrose transporter StSUT1 was experimentally determined to range at an unexpectedly low pH of 3 or even below. Various research...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.07.028
更新日期：2013-11-01 00:00:00
abstract：:Phospholipases A2 (PLA2) are a family of enzymes that catalyze the hydrolysis of the sn-2 ester bond of glycerophospholipids liberating lysophospholipids and free fatty acids; important second messengers involved in atherogenesis. Plasma PAF-acetylhydrolase (PAF-AH) or Lp-PLA2 is a Ca(2+)-independent PLA2 which is pro...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2010.02.002
更新日期：2010-06-01 00:00:00
abstract：:This review article outlines some potentials and limits of the recent application of high resolution Nuclear Magnetic Resonance technique--coupled to the Fourier transformation methods--to the study of biological membranes. Molecular arrangement and dynamical structure characters can be assessed at the level of indivi...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(75)80333-6
更新日期：1975-01-01 00:00:00
abstract：:Kex2 is a Ca2+-dependent serine protease from S. cerevisiae. Characterization of the substrate specificity of Kex2 is of particular interest because this protease serves as the prototype of a large family of eukaryotic subtilisin-related proprotein-processing proteases that cleave sites consisting of pairs or clusters...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2016.10.003
更新日期：2016-12-01 00:00:00
abstract：:A discriminating system capable of recognizing the oxygenated sterols was investigated in human lymphocytes. After labelling entire cells with 25-hydroxy [3H] cholesterol (10 nM) the cytosol was ultracentrifuged on a linear sucrose density gradient. Bound 25-hydroxy [3H] cholesterol was located in a single peak with a...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(82)80437-9
更新日期：1982-05-01 00:00:00
abstract：:Peptides with hormonal or neuronal activity are derived by enzymatic processing from pro-hormones, which by themselves are biologically inert. Processing and other enzymatic conversions may occur step-wise, leading to the formation of a cascade of biologically active (or inactive) peptides. The neurokin in substance P...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90159-9
更新日期：1988-01-01 00:00:00
abstract：:We have studied the kinetics of erythromycin inhibition of translation in growing bacteria. In order to simplify the interpretation of our data, we have used a mutant (envA), known to have an increased permeability to several antibiotics, including erythromycin. The data clearly show that an initial stage of translati...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90218-5
更新日期：1987-08-01 00:00:00
abstract：:Since 2012, the interest for TMEM165 increased due to its implication in a rare genetic human disease named TMEM165-CDG (Congenital Disorder(s) of Glycosylation). TMEM165 is a Golgi localized protein, highly conserved through evolution and belonging to the uncharacterized protein family 0016 (UPF0016). Although the pr...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.07.016
更新日期：2019-10-01 00:00:00
abstract：:Most evolutionists agree to consider that our present RNA/DNA/protein world has originated from a simpler world in which RNA played both the role of catalyst and genetic material. Recent findings from structural studies and comparative genomics now allow to get a clearer picture of this transition. These data suggest ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.03.015
更新日期：2005-09-01 00:00:00
abstract：:Pulmonary fibrosis (PF) is a progressive lethal disorder. In this study, the effect of daidzein, a soyisoflavone against Bleomycin (BLM) induced PF in rats was elucidated. A single intratracheal instillation of BLM (3 U/kg.bw) was administered in rats to induce PF. Daidzein (0.2 mg/kg) was administered subcutaneously,...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.04.005
更新日期：2014-08-01 00:00:00
abstract：:The targets of in vivo studies of the ribosomal assembly process are mainly the events of rRNA processing, whereas in vitro studies (total reconstitution) focus on principles of the assembly process such as assembly-initiation proteins, rate-limiting steps and a detailed sequence of assembly reactions (assembly map). ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90054-5
更新日期：1991-06-01 00:00:00
abstract：:Many human conditions, including neurological diseases, atherosclerosis, cancer, diabetic complications and aging, are thought to be associated with oxidative stress (OS). The development of reliable and informative markers for the characterization of OS in humans is thus highly important. Various endogenous markers a...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.03.005
更新日期：2013-03-01 00:00:00
abstract：:Three different hydrogenases (isolated from Clostridium pasteurianum, Desulfovibrio desulfuricans strain Norway 4 and D. baculatus 9974) added to a suspension of TiO2 (anatase) powder are able to catalyze H2 evolution under band gap illumination of the semiconducting particles, and in the presence of EDTA or methanol ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(86)81086-0
更新日期：1986-01-01 00:00:00
abstract：:The Escherichia coli Fis protein is a dimeric DNA-binding protein whose specific binding sites share a weak consensus sequence. Use of the gel retardation technique indicates that binding of Fis on a linear DNA fragment leads to the formation of a ladder of defined retarded complexes, independently of the presence of ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(94)90021-3
更新日期：1994-01-01 00:00:00
abstract：:The maximum activities of some key enzymes of metabolism were studied in lungs of fed and 48-h-starved rats. The maximum activity of hexokinase in the lung is similar to that of other tissues of the body, but lower than that of phosphorylase and 6-phosphofructokinase. High activities of glucose-6-phosphate dehydrogena...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90023-t
更新日期：1991-05-01 00:00:00
abstract：:tmRNA (10Sa RNA, ssrA) acts to rescue stalled bacterial ribosomes while encoding a peptide tag added trans-translationally to the nascent peptide, targeting it for proteolysis. Ribosomal protein S1 is required for tmRNA binding to isolated and poly U-programmed ribosomes. Mobility assays on native gels indicate that t...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(02)01442-6
更新日期：2002-08-01 00:00:00
abstract：:The field of immunometabolism has come a long way in the past decade, leading to the emergence of a new role for white adipose tissue (WAT) that is now recognized to stand at the junction of immune and metabolic regulations. Interestingly, a crucial role of the abundant and heterogeneous immune population present in W...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.06.012
更新日期：2016-05-01 00:00:00
abstract：:Nicotinate phosphoribosyltransferase (NaPRT, EC 2.4.2.11) catalyzes the conversion of nicotinate (Na) to nicotinate mononucleotide, the first reaction of the Preiss-Handler pathway for the biosynthesis of NAD(+). Even though NaPRT activity has been described to be responsible for the ability of Na to increase NAD(+) l...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.06.033
更新日期：2012-02-01 00:00:00
abstract：BACKGROUND:Fatty acids (FAs), and especially monounsaturated FAs (MUFAs) stimulate GLP-1 release. However, lipotoxicity is indicated in GLP-1 secreting cells following long-term exposure to elevated levels of saturated FAs (SFAs) in vivo and in vitro, where in vitro studies indicate that cosupplementation with MUFAs co...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.11.017
更新日期：2019-04-01 00:00:00
abstract：:The synthesis of S100 protein increases toward the end of the exponential phase of growth of clonal rat glial cells C6 in monolayer culture. Moreover the synthesis of this protein can be increased by treatment of C6 cells with the lectin succinylated concanavalin A (succinyl ConA). In order to study the relationship b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(84)80024-3
更新日期：1983-11-01 00:00:00
abstract：:A new apolipoprotein has been identified in VHDL1 and in HDL. This protein is immunologically distinct from already isolated apoproteins. It was isolated by column chromatography on hydroxylapatite. In polyacrylamide gel electrophoresis, its mobility is very close to that of apo D. The amino acid composition differs f...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(78)80199-0
更新日期：1978-01-01 00:00:00
abstract：:The rat and rabbit lung isoform pattern before (fetal) and after (adult) lung maturation has been analyzed by isoelectric focusing. This pattern has been compared to that of brain tubulin at the same developmental stages. No changes in the pattern of fetal and adult lung tubulin were found. However an increase in brai...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80301-1
更新日期：1985-09-01 00:00:00
abstract：:Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine biosynthetic pathway and has been exploited as the target for therapy against proliferative and parasitic diseases. In this study, we report the crystal structures of DHODH from Leishmania major, the species of Leishmania associated wi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.04.003
更新日期：2012-08-01 00:00:00
abstract：:The present review deals with the place of single chain oligonucleotide ligands (aptamers) in affinity chromatography applied to proteins. Aptamers are not the only affinity ligands available but they represent an emerging and highly promising route that advantageously competes with antibodies in immunopurification pr...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2017.10.008
更新日期：2018-02-01 00:00:00
abstract：:Conformational flexibility and structural fluctuations play an important role in enzyme activity. A great variety of internal motions ranging over different time scales and of different amplitudes are involved in the catalytic cycle. These different types of motions and their functional consequences are considered in ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(98)80054-0
更新日期：1998-01-01 00:00:00
abstract：:All hormones and neurotransmitters which provoke their intracellular effects by increasing the cytosolic concentration of Ca2+ in their target cells also stimulate the breakdown of inositol phospholipids. Much evidence suggests that this breakdown is intimately involved in the mechanism which couples cell-surface rece...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(85)80115-2
更新日期：1985-10-01 00:00:00
abstract：:The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central ner...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.12.008
更新日期：2020-03-01 00:00:00
abstract：:Specific contacts between DNA phosphate groups and positively charged nucleophilic amino acids from the Escherichia coli Fpg protein play a significant role in DNA-Fpg protein interaction. In order to identify these phosphate groups the chemical crosslinking procedure was carried out. The probing of the Fpg protein ac...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(03)00067-1
更新日期：2003-05-01 00:00:00
abstract：:Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases that process growth factors, growth factor binding proteins, cell surface proteins, degrade extracellular matrix (ECM) components and thereby play a central role in tissue remodeling and tumor progression. Membrane-type matrix metalloproteinases (MT-...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2004.07.012
更新日期：2005-03-01 00:00:00
abstract：:Beta-turmerin from turmeric (Curcuma longa) waste grits obtained after extraction of curcumin was purified by successive gel permeation chromatography. Homogeneity of beta-turmerin was confirmed by its movement as single band both in SDS-PAGE and as well as in native (basic) PAGE. The apparent molecular mass is approx...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.06.006
更新日期：2009-09-01 00:00:00
