Abstract:
:Nicotinate phosphoribosyltransferase (NaPRT, EC 2.4.2.11) catalyzes the conversion of nicotinate (Na) to nicotinate mononucleotide, the first reaction of the Preiss-Handler pathway for the biosynthesis of NAD(+). Even though NaPRT activity has been described to be responsible for the ability of Na to increase NAD(+) levels in human cells more effectively than nicotinamide (Nam), so far a limited number of studies on the human NaPRT have appeared. Here, extensive characterization of a recombinant human NaPRT is reported. We determined its major kinetic parameters and assayed the influence of different compounds on its enzymatic activity. In particular, ATP showed an apparent dual stimulation/inhibition effect at low/high substrates saturation, respectively, consistent with a negative cooperativity model, whereas inorganic phosphate was found to act as an activator. Among other metabolites assayed, including nucleotides, nucleosides, and intermediates of carbohydrates metabolism, some showed inhibitory properties, i.e. CoA, several acyl-CoAs, glyceraldehyde 3-phosphate, phosphoenolpyruvate, and fructose 1,6-bisphosphate, whereas dihydroxyacetone phosphate and pyruvate exerted a stimulatory effect. Furthermore, in light of the absence of crystallographic data, we performed homology modeling to predict the protein three-dimensional structure, and molecular docking simulations to identify residues involved in the recognition and stabilization of several ligands. Most of these residues resulted universally conserved among NaPRTs, and, in this study, their importance for enzyme activity was validated through site-directed mutagenesis.
journal_name
Biochimiejournal_title
Biochimieauthors
Galassi L,Di Stefano M,Brunetti L,Orsomando G,Amici A,Ruggieri S,Magni Gdoi
10.1016/j.biochi.2011.06.033subject
Has Abstractpub_date
2012-02-01 00:00:00pages
300-9issue
2eissn
0300-9084issn
1638-6183pii
S0300-9084(11)00230-6journal_volume
94pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Most evolutionists agree to consider that our present RNA/DNA/protein world has originated from a simpler world in which RNA played both the role of catalyst and genetic material. Recent findings from structural studies and comparative genomics now allow to get a clearer picture of this transition. These data suggest ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.03.015
更新日期:2005-09-01 00:00:00
abstract::LysK, the enzyme lysing cells of Staphylococcus aureus, can be considered as perspective antimicrobial agent. Knowledge of LysK properties and behavior would allow optimizing conditions of its storage as well as formulating strategy towards its stabilization. Reaction of LysK with substrate (suspension of autoclaved S...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.01.026
更新日期:2010-05-01 00:00:00
abstract::The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central ner...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.12.008
更新日期:2020-03-01 00:00:00
abstract::All hormones and neurotransmitters which provoke their intracellular effects by increasing the cytosolic concentration of Ca2+ in their target cells also stimulate the breakdown of inositol phospholipids. Much evidence suggests that this breakdown is intimately involved in the mechanism which couples cell-surface rece...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(85)80115-2
更新日期:1985-10-01 00:00:00
abstract::Ceramide synthases (CerSs) are key enzymes in the biosynthesis of ceramides and display a group of at least six different isoenzymes (CerS1-6). Ceramides itself are bioactive molecules. Ceramides with different N-acyl side chains (C(14:0)-Cer - C(26:0)-Cer) possess distinct roles in cell signaling. Therefore, the sele...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.09.007
更新日期:2012-02-01 00:00:00
abstract::The yeast Saccharomyces cerevisiae strain LB332 bearing a mutation in the ERG20 gene encoding farnesyl diphosphate synthase (FPPS) synthesizes significantly longer dolichols than the wild type strain FL100 (14-31 and 14-19 isoprene units, respectively). The measurement of the short chain prenyl alcohols excreted into ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)01155-x
更新日期:2000-08-01 00:00:00
abstract::Porphyromonas gingivalis uses a type IX secretion system (T9SS) to deliver more than 30 proteins to the bacterial surface using a conserved C-terminal domain (CTD) as an outer membrane translocation signal. On the surface, the CTD is cleaved and an anionic lipopolysaccharide (A-PLS) is attached by PorU sortase. Among ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.06.010
更新日期:2019-11-01 00:00:00
abstract::Coumarins are a group of natural compounds widely distributed in plants. Of late, coumarins and their derivatives have grabbed much attention from the pharmacological and pharmaceutical arena due to their broad range of therapeutical qualities. A coumarin derivative 4-methylesculetin (4-ME) has known to possess effect...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.02.014
更新日期:2013-06-01 00:00:00
abstract::Translational autocontrol of the expression of the ribosomal protein S15 proceeds through the transitory formation of a pseudoknot. A synopsis of the known data is used to propose a molecular model of the mechanism involved and for the role of the pseudoknot. This latter structure is able to recruit 30S ribosomal subu...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(96)80003-4
更新日期:1996-01-01 00:00:00
abstract::Influenza virus-associated RNA polymerase is composed of one molecule each of three viral P proteins and carries the complete activity of capped RNA-primed vRNA-directed transcription. The RNA polymerase holoenzyme also carries capped RNA endonuclease to generate capped oligonucleotide primers for transcription and 3'...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(97)86735-1
更新日期:1996-01-01 00:00:00
abstract::Versican and decorin, two proteoglycans (PGs) with contradictory roles in the pathophysiology of cancer, comprise important stromal components in many tumor types and play a crucial role in the progression of cancer. In this study, we provide direct evidence for a significant and stage-related accumulation of versican...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.03.011
更新日期:2006-09-01 00:00:00
abstract::In this contribution, we have studied the dynamics of electron transfer (ET) of a flavoprotein to the bound cofactor, an important metabolic process, in a model molecular/macromolecular crowding environments. Vitamin B(2) (riboflavin, Rf) and riboflavin binding protein (RBP) are used as model cofactor and flavoprotein...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.08.005
更新日期:2012-12-01 00:00:00
abstract::Here we describe a convenient and robust ligase-independent method for construction of combinatorial and random mutant libraries. The homologous genes flanked by plasmid-derived DNA sequences are fragmented, and the random fragments are reassembled in a self-priming polymerase reaction to obtain chimeric genes. The pr...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.03.016
更新日期:2010-08-01 00:00:00
abstract::The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90006-x
更新日期:1994-01-01 00:00:00
abstract::Evidence shows that oral glycerol supplementation during the early stages of rat liver cancer reduces the growth of preneoplastic lesions. Besides, human hepatocellular carcinoma (HCC) cells display decreased expression of glycerol channel aquaporin 9 (AQP9) and also diminished glycerol-3-phosphate (G3P) content. Acco...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.01.002
更新日期:2020-03-01 00:00:00
abstract::The present study was conducted on CD4(+) T cells, isolated from wild type (WT) and PPARalpha(null) mice, in order to assess the mechanism of action of docosahexaenoic acid (DHA), an n-3 fatty acid, in the modulation of two transcription factors, i.e., T-bet and GATA-3, implicated in T-cell differentiation towards, re...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.09.012
更新日期:2009-11-01 00:00:00
abstract::A Binding Protein (BP) of nearly 95,000 daltons interacts with the acid phosphatase at the permissive temperature, elevating its molecular weight from 48,000 +/- 2,000 to 136,000 +/- 7,000 in CL and CHR strains of Physarum polycephalum plasmodia. The same molecule has not been observed in the extracts of M3C VIII and ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(83)80160-6
更新日期:1983-06-01 00:00:00
abstract::Some oxysterols resulting either from enzymatic oxidation or autoxidation of cholesterol are associated with age-related diseases including neurodegenerative diseases. Among these oxysterols, 7β-hydroxycholesterol (7β-OHC) is often found at increased levels in patients. It is therefore important to identify molecules ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.06.027
更新日期:2018-10-01 00:00:00
abstract::Disappearance of 7-ethylguanine and O6-ethylguanine lesions from nuclear DNA is observed on incubation of isolated rat liver nuclei previously treated with ethylnitrosourea. Free 7-ethylguanine but no free O6-ethylguanine is released in the medium. Incubation of methylated or ethylated DNA with chromatin proteins lead...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80132-6
更新日期:1982-08-01 00:00:00
abstract::A lignin model compound, named in short guaiagylglycerol beta-guaiacyl ether (GGE), contains the beta-0-4 ether linkage that is common in the chemical structure of lignin. A Pseudomonas sp. (GU5) had been isolated as an organism able to grow with GGE as the sole source of carbon and energy. When grown on vanillate, th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80292-3
更新日期:1985-09-01 00:00:00
abstract::Ton-dependent colicins and microcins are actively taken up into sensitive cells at the expense of energy which is provided by the proton motive force of the cytoplasmic membrane. The Ton system consisting of the proteins TonB, ExbB and ExbD is required for colicin and microcin import. Colicins as well as the outer mem...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(02)01427-x
更新日期:2002-05-01 00:00:00
abstract::Crystals of flavoenzyme L-lactate oxidase from Aerococcus viridans (LOX) have been obtained that diffract to 3.0 A resolution (P2(1)2(1)2(1), a = 118.4 A, b = 138.4 A, c = 194.6 A). Crystallographic studies suggest that the enzyme may exist as an octameric form with non-crystallographic two- and four-fold axes in the ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(98)80072-2
更新日期:1998-04-01 00:00:00
abstract::After the assessment of the antitumor activity of the anthracycline pigments, the S peucetius group of metabolites was discovered and eventually doxorubicin, a major anticancer agent of established clinical usefulness was developed in the early seventies. A second generation of compounds followed, represented mainly b...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(98)80003-5
更新日期:1998-03-01 00:00:00
abstract::The RecA protein of E coli promotes a strand exchange reaction in vitro which appears to be similar to homologous genetic recombination in vivo. A model for the mechanism of strand transfer reaction by RecA protein has been proposed by Howard-Flanders et al based on the assumption that the RecA monomer has two distinc...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90200-k
更新日期:1991-02-01 00:00:00
abstract::The effects of thermal injury (72 h post-injury), 72 h-partial (20% less food) or full starvation on the regulation of phosphofructokinase in the mucosa of rat small intestine were studied. Thermal injury and 72 h-partial or full starvation decreased the activity ratio v0.5/V, but the ratios obtained for thermally inj...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(87)90273-2
更新日期:1987-01-01 00:00:00
abstract::Knowing the structure of a protein is essential to characterize its function and mechanism at the molecular level. Despite major advances in solving structures experimentally, most membrane protein native conformations remain unknown. This lack of available structures, along with the physical constraints imposed by th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.05.018
更新日期:2015-08-01 00:00:00
abstract::The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80316-7
更新日期:1977-01-01 00:00:00
abstract::The lipoprotein complex from human gallbladder bile was challenged with anti-IgA and anti-IgG antisera in order to determine whether the apolipoprotein complex isolated from the detergent-free form of bile lipoprotein contains IgA and IgG. The apolipoprotein complex indeed crossreacts with anti-IgA and anti-IgG. In ad...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(81)80033-8
更新日期:1981-10-01 00:00:00
abstract:BACKGROUND:Fatty acids (FAs), and especially monounsaturated FAs (MUFAs) stimulate GLP-1 release. However, lipotoxicity is indicated in GLP-1 secreting cells following long-term exposure to elevated levels of saturated FAs (SFAs) in vivo and in vitro, where in vitro studies indicate that cosupplementation with MUFAs co...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.11.017
更新日期:2019-04-01 00:00:00
abstract::We have previously shown that withdrawal of folic acid led to metabolic reprogramming and a less aggressive phenotype in a mouse cell model of triple-negative breast cancer (TNBC). Herein, we evaluate the effects of folic acid withdrawal on transcriptomic profiles in these cells. Murine cell lines were originally deri...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.04.005
更新日期:2020-06-01 00:00:00