Abstract：
:In this contribution, we have studied the dynamics of electron transfer (ET) of a flavoprotein to the bound cofactor, an important metabolic process, in a model molecular/macromolecular crowding environments. Vitamin B(2) (riboflavin, Rf) and riboflavin binding protein (RBP) are used as model cofactor and flavoprotein, respectively. An anionic surfactant sodium dodecyl sulfate (SDS) is considered to be model crowding agent. A systematic study on the ET dynamics in various SDS concentration, ranging from below critical micellar concentration (CMC), where the surfactants remain as monomeric form to above CMC, where the surfactants self-assemble to form nanoscopic micelle, explores the dynamics of ET in the model molecular and macromolecular crowding environments. With energy selective excitation in picosecond-resolved studies, we have followed temporal quenching of the tryptophan residue of the protein and Rf in the RBP-Rf complex in various degrees of molecular/macromolecular crowding. The structural integrity of the protein (secondary and tertiary structures) and the vitamin binding capacity of RBP have been investigated using various techniques including UV-Vis, circular dichroism (CD) spectroscopy and dynamic light scattering (DLS) studies in the crowding environments. Our finding suggests that the effect of molecular/macromolecular crowding could have major implication in the intra-protein ET dynamics in cellular environments.
journal_name
Biochimiejournal_title
Biochimieauthors
Rakshit S,Saha R,Verma PK,Mitra RK,Pal SKdoi
10.1016/j.biochi.2012.08.005subject
Has Abstractpub_date
2012-12-01 00:00:00pages
2673-80issue
12eissn
0300-9084issn
1638-6183pii
S0300-9084(12)00329-Xjournal_volume
94pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Single cell oils (SCOs) are now produced by various microorganisms as commercial sources of arachidonic acid (ARA) and docosahexaenoic acid (DHA). These oils are now used extensively as dietary supplements in infant formulas. An understanding of the underlying biochemistry and genetics of oil accumulation in such micr...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2004.09.017
更新日期：2004-11-01 00:00:00
abstract：:Sialic acid, a nine-carbon sugar acid usually is present in the non-reducing terminal position of free oligosaccharides and glycoconjugates. Sialylated conjugates in mammals perform important roles in cellular recognition, signaling, host-pathogen interaction and neuronal development. Metabolism of sialylated conjugat...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2011.06.002
更新日期：2011-10-01 00:00:00
abstract：:Excessive tissue iron levels are associated with the increase of oxidative/nitrative stress which contributes to tissue damage that may elevate the risk of diabetes. Therefore, we investigated the effects of iron on diabetes-associated liver injury and whether iron-related tyrosine nitration participated in this proce...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.07.019
更新日期：2012-12-01 00:00:00
abstract：:(-)-Epigallocatechin-3-gallate (EGCG), a well-known chemopreventive factor, induces cancer cells undergoing apoptosis. Over the last several years, we have shown that the mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) functions as an antioxidant and anti-apoptotic protein by supplying NADPH to antioxi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.06.025
更新日期：2011-10-01 00:00:00
abstract：:Previous reports on the carbohydrate specificities of Amaranthus caudatus lectin (ACL) and peanut agglutinin (PNA, Arachis hypogea) indicated that they share the same specificity for the Thomsen-Friedenreich (T(alpha), Galbeta1-3GalNAcalpha1-Ser/Thr) glycotope, but differ in monosaccharide binding--GalNAc>Gal (inactiv...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.08.001
更新日期：2008-11-01 00:00:00
abstract：:The complete amino acid sequences of 3 proteins from the 50S subunit of Bacillus stearothermophilus ribosomes were determined by N-terminal sequence analysis and by sequencing of overlapping fragments obtained from enzymatic digestions and chemical cleavages. The proteins BstL28, BstL33 and BstL34, named according to ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90126-l
更新日期：1991-07-01 00:00:00
abstract：:Epigenetic mechanisms are likely to play a role in many complex diseases, the extent of which we only beginning to understand. COPD and asthma are two respiratory diseases subject to strong environmental influences depending on underlying genetic susceptibility. Epigenetic mechanisms such as DNA methylation, histone m...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.07.017
更新日期：2012-11-01 00:00:00
abstract：:LysK, the enzyme lysing cells of Staphylococcus aureus, can be considered as perspective antimicrobial agent. Knowledge of LysK properties and behavior would allow optimizing conditions of its storage as well as formulating strategy towards its stabilization. Reaction of LysK with substrate (suspension of autoclaved S...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.01.026
更新日期：2010-05-01 00:00:00
abstract：:Over the past 200 years emissions of toxic heavy metals have risen tremendously and significantly exceed those from natural sources for practically all metals. Uptake and accumulation by crop plants represents the main entry pathway for potentially health-threatening toxic metals into human and animal food. Of major c...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2006.07.003
更新日期：2006-11-01 00:00:00
abstract：:The hepatitis E virus (HEV) is the most common cause of acute hepatitis worldwide. Although HEV is a small, naked RNA virus, HEV particles become associated with lipids in the blood of infected patients and in the supernatant of culture systems. The egress of these particles from cells implies the exocytosis pathway b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.05.003
更新日期：2017-10-01 00:00:00
abstract：:Thraustochytrium is a marine protist producing a specific profile of nutritionally important fatty acids, including very long chain polyunsaturated fatty acids (VLCPUFAs) docosahexaenoic acid (DHA, 22:6n-3), even chain saturated fatty acids (SFAs) palmitic acid (16:0), and odd chain SFAs pentadecanoic acid (15:0). To ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.10.024
更新日期：2018-01-01 00:00:00
abstract：:Beta-turmerin from turmeric (Curcuma longa) waste grits obtained after extraction of curcumin was purified by successive gel permeation chromatography. Homogeneity of beta-turmerin was confirmed by its movement as single band both in SDS-PAGE and as well as in native (basic) PAGE. The apparent molecular mass is approx...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.06.006
更新日期：2009-09-01 00:00:00
abstract：:The toxicity of amyloids is a subject under intense scrutiny. Many studies link this toxicity to the existence of various intermediate structures prior to the fiber formation and/or their specific interaction with membranes. Membranes can also be a catalyst of amyloidogenesis and the composition or the charge of membr...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.07.011
更新日期：2013-01-01 00:00:00
abstract：:Insulin stimulates protein synthesis in skeletal muscle of young animals and has been reported to exert similar effects on a variety of mammalian cell types in culture. However, with chick embryo fibroblasts we found that the extent of this effect was very sensitive to the culture conditions of the cells prior to the ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90129-g
更新日期：1993-01-01 00:00:00
abstract：:IbpA/B, 16 kDa heat-shock proteins were recently described as recognizing heterologous protein inclusion bodies in Escherichia coli cells; the corresponding genes formed an operon regulated by the rpoH gene product, sigma 32 protein (Burland et al (1993) Genomics 16, 551; Allen et al (1992) J Bacteriol 174, 6938; Chua...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(96)82643-5
更新日期：1996-01-01 00:00:00
abstract：:The incubation of polyphosphates with the ribosomal fraction of Sulfolobus acidocaldarius leads to the covalent attachment of phosphate to threonine residue(s) of a single 40,000 Mr protein. The hydrolysis kinetics of this protein showed that polyphosphate might be the modifying group. The reaction requires 2 mM Mn2+ ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(89)90115-6
更新日期：1989-09-01 00:00:00
abstract：:To elucidate the metabolic function of mRNA polyadenylation in Escherichia coli. we searched for a polyadenylate-binding protein as a potential mediator of the function of the poly(A) moiety. Using a nitrocellulose filter-binding assay and a Northwestern blot technique, a protein in the ribosomal supernatant fraction ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)82741-1
更新日期：1997-09-01 00:00:00
abstract：:The effect of curing agents (salt, nitrate, ascorbic acid and glucose) and processing parameters (pH, water activity and drying and cooking temperatures) on pork muscle cathepsins B, D, H and L as well as leucyl, arginyl and tyrosyl hydrolysing activities is reported. Salt (60 g/l) showed a powerful inhibitory effect,...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(92)90128-2
更新日期：1992-03-01 00:00:00
abstract：:Peroxisomes are subcellular organelles which are present in virtually every eukaryotic cell and catalyze a large number of metabolic functions. The importance of peroxisomes for humans is stressed by the existence of a large group of genetic diseases in which either the biogenesis of peroxisomes is impaired or one of ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2013.08.022
更新日期：2014-03-01 00:00:00
abstract：:Mitochondrial membranes are essential for the good functioning of the organelle. For instance, the inner mitochondrial membrane contains the oxidative phosphorylation system that permits ATP synthesis. Phospholipids environment and especially cardiolipin are crucial for the mitochondrial energy metabolism. Indeed, car...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.07.009
更新日期：2013-01-01 00:00:00
abstract：:A thrombin-like enzyme, named BjussuSP-I, isolated from Bothrops jararacussu snake venom, is an acidic single-chain glycoprotein with M(r)=61,000, pI approximately 3.8 and 6% sugar. BjussuSP-I shows high proteolytic activity upon synthetic substrates, such as S-2238 and S-2288. It also shows procoagulant and kallikrei...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.10.005
更新日期：2008-03-01 00:00:00
abstract：:Long non-coding RNAs constitute the most abundant part of the transcribed mammalian genome. lncRNAs affect all essential processes in the living cell including transcription, splicing, translation, replication, shaping of chromatin and post translational modification of proteins. Alterations in lncRNA expression have ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2016.06.007
更新日期：2016-12-01 00:00:00
abstract：:Parkinson's disease (PD), a neurodegenerative disorder, is characterized by a loss of dopaminergic neurons in the substantia nigra (SN) of the brain and it is well known that the pathogenesis of PD is related to a number of risk factors including oxidative stress. Antioxidant 1 (ATOX1) protein plays a crucial role in ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.10.010
更新日期：2019-01-01 00:00:00
abstract：:Pterocarpans, a family of isoflavonoids found in the diverse Fabaceae, display potent cytotoxic activity over a panel of tumor cell lines, and among those tested, 2,3,9- trimethoxypterocarpan displays the most potent activity. This study evaluates the effects of 2,3,9-trimethoxypterocarpan and its related derivatives ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.06.005
更新日期：2014-09-01 00:00:00
abstract：:Staphylococcus aureus is one of the most dreaded pathogens worldwide and emergence of notorious antibiotic resistant strains have further exacerbated the present scenario. The glycolytic enzyme, triosephosphate isomerase (TIM) is one of the cell envelope proteins of the coccus and is involved in biofilm formation. It ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.07.001
更新日期：2012-12-01 00:00:00
abstract：:We have studied the kinetics of erythromycin inhibition of translation in growing bacteria. In order to simplify the interpretation of our data, we have used a mutant (envA), known to have an increased permeability to several antibiotics, including erythromycin. The data clearly show that an initial stage of translati...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90218-5
更新日期：1987-08-01 00:00:00
abstract：:The thrombin-binding DNA aptamer (TBA) 5'-d(GGTTGGTGTGGTTGG)-3' forms a G-quadruplex that is necessary for binding to the coagulation factor thrombin. The stability of the G-quadruplex of TBA when bound to thrombin and potassium ion (K(+)) were investigated for the wild-type oligonucleotide and for mutants in which th...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.03.013
更新日期：2011-08-01 00:00:00
abstract：:About 150 participants attended the symposium organised at the Palais de la Bourse in Bordeaux, France on September 22-23, 2017. Thirty speakers from all over the world delivered lectures covering selection processes, aptamer chemistry and innovative applications of these powerful tools that display major advantages o...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.11.015
更新日期：2018-02-01 00:00:00
abstract：:As the medaka is a popular fish model in genetics, developmental biology and toxicology, the development of an efficient transgenic medaka technique is important for a variety of biological experiments. Here we demonstrated that the maize transposon system, Ac/Ds, greatly improved the transgenesis of microinjected DNA...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.07.006
更新日期：2011-10-01 00:00:00
abstract：:Bacterial type II fatty acid synthesis (FASII) is a target for novel antibiotic development. All bacteria encode for mechanisms to incorporate exogenous fatty acids, and some bacteria can use exogenous fatty acids to bypass FASII inhibition. Bacteria encode three different mechanisms for activating exogenous fatty aci...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2017.06.015
更新日期：2017-10-01 00:00:00
