Fatty acid biosynthesis in microorganisms being used for Single Cell Oil production.

abstract：:Although a 30 kDa polypeptide frequently is seen by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of post mortem (pm) skeletal muscle and in turn is used as an indicator of proteolysis, its origin has not been conclusively identified. We used antibodies to selected myofibrillar proteins...

journal_title：Biochimie

authors： Ho CY,Stromer MH,Robson RM

更新日期：1994-01-01 00:00:00

abstract：:I have been involved in research on oxysterols since 1963 and this review is intended to cover some of the most important aspects of this work. The first project dealed with 7α-hydroxy-4-cholesten-3-one. My successful synthesis of this steroid with high specific radioactivity allowed a demonstration that it is a bile ...

journal_title：Biochimie

authors： Björkhem I

更新日期：2013-03-01 00:00:00

abstract：:The elucidation of the multiple signaling cascades coupled to the TSH receptor has offered new approaches in the understanding of the pathogenesis of Graves' disease. Here we review findings showing that immunoglobulins from Graves' patients are heterogeneous, bind to different epitopes and, similarly to TSH, activate...

journal_title：Biochimie

authors： Di Cerbo A,Corda D

更新日期：1999-05-01 00:00:00

abstract：:The chromatin of shrimp hepatopancreas has been extracted from isolated nuclei and characterized. Nuclei were prepared in the presence of Cu++ and phenyl methyl sulfonyl fluoride in order to inhibit the nuclease and protease activities throughout the different purification steps. The purified nuclei are heterogenous i...

journal_title：Biochimie

authors： Sellos D,Van Wormhoudt A

更新日期：1979-01-01 00:00:00

abstract：:Inducing brown adipocytes in white adipose tissues is a promising target to combat obesity and its related disorders in human beings. This goal has been especially encouraged by new important discoveries of human brown adipose tissues. The accumulating evidence confirms the presence of active brown adipocytes, not onl...

journal_title：Biochimie

authors： Chu DT,Gawronska-Kozak B

更新日期：2017-07-01 00:00:00

abstract：:A non-toxic, direct-acting fibrinolytic serine protease (Bafibrinase) demonstrating thrombolytic and anticoagulant properties was purified from Bacillus sp. strain AS-S20-I. Bafibrinase was monomeric, with a molecular mass of 32.3 kDa. The peptide mass fingerprinting of Bafibrinase revealed only 8.3% sequence coverage...

journal_title：Biochimie

authors： Mukherjee AK,Rai SK,Thakur R,Chattopadhyay P,Kar SK

更新日期：2012-06-01 00:00:00

abstract：:Pseudouridine [Ψ] is a frequent base modification in the ribosomal RNA [rRNA] and may be involved in the modulation of the conformational flexibility of rRNA helix-loop structures during protein synthesis. Helix 69 of 23S rRNA contains pseudouridines at the positions 1911, 1915 and 1917 which are formed by the helix 6...

journal_title：Biochimie

authors： Kipper K,Sild S,Hetényi C,Remme J,Liiv A

更新日期：2011-05-01 00:00:00

abstract：:Diacylglycerol kinase (DGK) phosphorylates diacylglycerol to produce phosphatidic acid (PA). The η isozyme of DGK is abundantly expressed in C2C12 myoblasts. However, the role of DGKη in skeletal muscle cells remains unknown. In the present study, we showed that DGKη was downregulated at an early stage of myogenic dif...

journal_title：Biochimie

authors： Sakai H,Murakami C,Usuki T,Lu Q,Matsumoto KI,Urano T,Sakane F

更新日期：2020-10-01 00:00:00

abstract：:The effects of various concentrations of 2-fluoro-2-deoxy-D-glucose (FDG) on the aerobic metabolism of glucose and the reciprocal effect of glucose on the metabolism of FDG in glucose-grown repressed Saccharomyces cerevisiae cells were studied at 30 degrees C in a standard pyrophosphate medium containing 5 x 10(7) cel...

journal_title：Biochimie

authors： Tran-Dinh S,Courtois A,Wietzerbin J,Bouet F,Herve M

更新日期：1995-01-01 00:00:00

abstract：:A synthetic phosphonate inhibitor designed for lipase inhibition but displaying a broader range of activity was covalently immobilized on a solid support to generate a function-directed tool targeting serine hydrolases. To achieve this goal, straightforward and reliable analytical techniques were developed, allowing t...

journal_title：Biochimie

authors： Delorme V,Raux B,Puppo R,Leclaire J,Cavalier JF,Marc S,Kamarajugadda PK,Buono G,Fotiadu F,Canaan S,Carrière F

更新日期：2014-12-01 00:00:00

abstract：:Interleukin-17 (IL-17) is a pro-inflammatory cytokine produced primarily by a subset of CD4(+)T cells, called Th17 cells, that is involved in host defense, inflammation and autoimmune disorders. The two most structurally related IL-17 family members, IL-17A and IL-17F, form homodimeric (IL-17A/A, IL-17F/F) and heterod...

journal_title：Biochimie

authors： Adachi H,Ishiguro A,Hamada M,Sakota E,Asai K,Nakamura Y

更新日期：2011-07-01 00:00:00

abstract：:We have studied through the late foetal period of the rat, the evolution of two enzymes involved in glycogen metabolism of the liver : UDPG glycogen synthetase (a and b forms) and branching enzyme. The activities were measured during the development of normal foetuses and of foetuses experimentally deprived of cortico...

journal_title：Biochimie

authors： Gilbert M,Vaillant R

更新日期：1975-01-01 00:00:00

abstract：:The cartilage tissue has a limited self-regenerative capacity. Tissue-engineering represents a promising trend for cartilage repair. The present study was aimed to develop a biomaterial formulation by combining fragments of chitosan hydrogel with isolated rabbit or human chondrocytes. We first reported the properties ...

journal_title：Biochimie

authors： Montembault A,Tahiri K,Korwin-Zmijowska C,Chevalier X,Corvol MT,Domard A

更新日期：2006-05-01 00:00:00

abstract：:Adipose tissue lipoprotein lipase activity (LPL) falls dramatically during experimental infection of mice by Plasmodium chabaudi. The decrease in this activity accounts for the seric accumulation of triacyl-glyceride-rich lipoproteins (chylomicrons mainly and Very Low Density Lipoproteins (VLDL) partly). This loss of ...

journal_title：Biochimie

authors： Al Rifai W,Maurois P,Fruchart JC,Charet P

更新日期：1982-02-01 00:00:00

abstract：:Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric ...

journal_title：Biochimie

authors： Cafaro V,Bracale A,Formiggini F,Notomista E,D'Alessio G,Di Donato A

更新日期：1998-11-01 00:00:00

abstract：:The chemical carcinogen N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) induces frameshift mutations located within two types of specific sequences (mutation hot spots): i) contiguous guanine sequences and ii) alternating GC sequences. The genetic requirements of these frameshift events were investigated using specific ...

journal_title：Biochimie

authors： Janel-Bintz R,Maenhaut-Michel G,Takahashi M,Fuchs RP

更新日期：1991-04-01 00:00:00

abstract：:A major incentive in inhibitor research is that control of limited proteolysis constitutes a valuable pharmacological tool. Protease inhibitors have proved to be successful in influencing pathogenesis in many experimental models but a breakthrough to use in human therapy has mainly been restricted to aprotinin and ang...

journal_title：Biochimie

authors： Scharpe S,De Meester I,Hendriks D,Vanhoof G,van Sande M,Vriend G

更新日期：1991-01-01 00:00:00

abstract：:We have produced and purified an active site mutant of the Escherichia coli cyclopropane fatty acid synthase (CFAS) by replacing the strictly conserved G236 within cyclopropane synthases, by a glutamate residue, which corresponds to E146 of the homologous mycolic acid methyltransferase, Hma, producing hydroxymethyl my...

journal_title：Biochimie

authors： E G,Drujon T,Correia I,Ploux O,Guianvarc'h D

更新日期：2013-12-01 00:00:00

abstract：:High glucose (HG)-induced podocyte injury contributes to the pathogenesis of diabetic nephropathy, a severe complication of diabetes. Bromodomain-containing protein 4 (BRD4) has emerged as a critical regulator for cell injury. However, whether BRD4 participates in HG-induced podocyte injury remains unclear. In this st...

journal_title：Biochimie

authors： Zuo H,Wang S,Feng J,Liu X

更新日期：2019-10-01 00:00:00

abstract：:tmRNA (10Sa RNA, ssrA) acts to rescue stalled bacterial ribosomes while encoding a peptide tag added trans-translationally to the nascent peptide, targeting it for proteolysis. Ribosomal protein S1 is required for tmRNA binding to isolated and poly U-programmed ribosomes. Mobility assays on native gels indicate that t...

journal_title：Biochimie

authors： Bordeau V,Felden B

更新日期：2002-08-01 00:00:00

abstract：:Using a new extended set of 13 amber suppressors in E coli, systematic amino-acid replacements were performed at positions 104(E) and 238(G) of TEM-1 beta-lactamase from PUC19. The enzyme is tolerant to most substitutions tested at position 104. Missense revertants E104K, E104S or E104Y exhibited only minor changes in...

journal_title：Biochimie

authors： Lenfant F,Labia R,Masson JM

更新日期：1990-06-01 00:00:00

abstract：:Tetramolecular G-quadruplexes result from the association of four guanine-rich strands. Modification of the backbone strand or the guanine bases of the oligonucleotide may improve stability or introduce new functionalities. In this regard, the 8 position of a guanosine is particularly suitable for introduction of modi...

journal_title：Biochimie

authors： Tran PL,Virgilio A,Esposito V,Citarella G,Mergny JL,Galeone A

更新日期：2011-03-01 00:00:00

abstract：:Multiple forms of acetylcholinesterase have been studied in Goldfish adapted to 15 degrees C and 25 degrees C. Three forms of enzyme (sedimenting at 18S, 12S and 5S) could be distinguished by sucrose gradient centrifugation. Their molecular and enzymatic properties did not vary with environmental temperature. It was n...

journal_title：Biochimie

authors： Guillon G,Massoulié J,Guillon G,Massoulié J

更新日期：1976-01-01 00:00:00

abstract：:We present here, a new method for coupling sulfhydryl groups (SH) to low-density lipoprotein (LDL) surface. This method uses homocysteine thiolactone (HCTL) which reacts with lysine residues in a very mild manner, and permits the selection of the number of SH bound per LDL. Under our experimental conditions (8 SH/LDL)...

journal_title：Biochimie

authors： Vidal M,Sainte-Marie J,Philippot J,Bienvenue A

更新日期：1986-05-01 00:00:00

abstract：:The reactivity of sonicated phosphatidylcholine-cholesterol liposomes with cholesterol : oxygene oxydoreductase, an enzyme which catalyses the oxidation of the 3 beta hydroxyl group of cholesterol to a ketone group, is compared with that of ternary system phosphatidylcholine-cholesterol-Thesit. Regardless to the phosp...

journal_title：Biochimie

authors： Peynet J,Delattre J,Canal J,Rousselet F,Girard ML

更新日期：1979-01-01 00:00:00

abstract：:Seryl-tRNA synthetases (SerRS) covalently attach serine to cognate tRNA(Ser). Atypical SerRSs, considerably different from canonical enzymes, have been found in methanogenic archaea. A crystal structure of methanogenic-type SerRS revealed a motif within the active site (serine ordering loop; SOL), which undergoes a no...

journal_title：Biochimie

authors： Dulic M,Pozar J,Bilokapic S,Weygand-Durasevic I,Gruic-Sovulj I

更新日期：2011-10-01 00:00:00

abstract：:1)Individual monosaccharides (uronic acids and aminosugars) have been purified following specific hydrolysis of the mucopolysaccharides from new born rat skin (hyaluronic acid, heparin + heparan sulfate, chondroitin sulfate A, B and C), after incubation with [U14C]-glucose under various conditions and for varying incu...

journal_title：Biochimie

authors： Hervé B,Clauser H

更新日期：1975-11-01 00:00:00

abstract：:Three different hydrogenases (isolated from Clostridium pasteurianum, Desulfovibrio desulfuricans strain Norway 4 and D. baculatus 9974) added to a suspension of TiO2 (anatase) powder are able to catalyze H2 evolution under band gap illumination of the semiconducting particles, and in the presence of EDTA or methanol ...

journal_title：Biochimie

authors： Cuendet P,Rao KK,Grätzel M,Hall DO

更新日期：1986-01-01 00:00:00

abstract：:Living organisms are constantly exposed to oxidative stress from environmental agents and from endogenous metabolic processes. The resulting oxidative modifications occur in proteins, lipids and DNA. Since proteins and lipids are readily degraded and resynthesized, the most significant consequence of the oxidative str...

journal_title：Biochimie

authors： Bohr VA,Dianov GL

更新日期：1999-01-01 00:00:00

abstract：:Hydroxylamine and its derivatives of general formula H2NOR react with aldehydes and aldimines to produce oximes. If R corresponds to the side chain of a natural amino acid, such compounds can be thought of as analogs of the corresponding amino acids, lacking the alpha-carboxylate group. Oximes formed between such comp...

journal_title：Biochimie

authors： Delbaere LT,Kallen J,Markovic-Housley Z,Khomutov AR,Khomutov RM,Karpeisky MY,Jansonius JN

更新日期：1989-04-01 00:00:00