Reciprocal effects of 2-fluoro-2-deoxy-D-glucose and glucose on their metabolism in Saccharomyces cerevisiae studied by multi-nuclear NMR spectroscopy.


:The effects of various concentrations of 2-fluoro-2-deoxy-D-glucose (FDG) on the aerobic metabolism of glucose and the reciprocal effect of glucose on the metabolism of FDG in glucose-grown repressed Saccharomyces cerevisiae cells were studied at 30 degrees C in a standard pyrophosphate medium containing 5 x 10(7) cells/ml by 1H-, 19F-, 31P-NMR and biochemical techniques. The glucose consumption rate is reduced by about 57% and 71% in the presence of 5 mM FDG and 10 mM FDG respectively. Under the same conditions, the ethanol production rate also decreases about 54% and 68%, respectively. When FDG is the unique carbon source, the alpha- and beta-anomers of 2-fluoro-2-deoxy-D-glucose-6-phosphate (FDG6P) and a much smaller quantity of 2-fluoro-2-deoxy-gluconic acid (FDGA) were observed. The quantities of alpha- and beta-FDG6P reach their maximum values within 1 h of incubation and then decrease continuously. In contrast, Glc favors the consumption of FDG and the synthesis of FDG6P and uridine-5'-diphosphate fluorodeoxy-glucose (UDP-FDG). In the presence of Glc, FDG6P reaches a plateau after 1 h or 2 h of incubation while UDP-FDG increases regularly with time. Apart from trehalose, no other disaccharide such as fluoro-dideoxy-trehalose (FDG-FDG) or fluoro-deoxy-trehalose (FDG-Glc) were observed. Thus, in contrast to UDP-Glc, UDP-DG, Glc6P and DG6P, UDP-FDG and FDG6P are not good substrates for trehalose-6-P synthetase.(ABSTRACT TRUNCATED AT 250 WORDS)






Tran-Dinh S,Courtois A,Wietzerbin J,Bouet F,Herve M




Has Abstract


1995-01-01 00:00:00














  • Deimination of glycogen phosphorylase b by peptidylarginine deiminase. Influence on the kinetical characteristics and dimer-tetramer transition.

    abstract::The kinetics of the native glycogen phosphorylase b from rabbit skeletal muscle and of the enzyme specifically deiminated by peptidylarginine deiminase have been studied. One arginine residue per phosphorylase b monomer is transformed into citrulline after 3 h of incubation with peptidylarginine deiminase. The maximal...


    pub_type: 杂志文章


    authors: Eronina TB,Livanova NB,Chebotareva NA,Kurganov BI,Luo S,Graves DJ

    更新日期:1996-01-01 00:00:00

  • Effects of 8-methylguanine on structure, stability and kinetics of formation of tetramolecular quadruplexes.

    abstract::Tetramolecular G-quadruplexes result from the association of four guanine-rich strands. Modification of the backbone strand or the guanine bases of the oligonucleotide may improve stability or introduce new functionalities. In this regard, the 8 position of a guanosine is particularly suitable for introduction of modi...


    pub_type: 杂志文章


    authors: Tran PL,Virgilio A,Esposito V,Citarella G,Mergny JL,Galeone A

    更新日期:2011-03-01 00:00:00

  • Crosslinking of eukaryotic initiation factor eIF3 to the 40S ribosomal subunit from rabbit reticulocytes.

    abstract::Complexes of purified 40S ribosomal subunits and initiation factor 3 from rabbit reticulocytes were crosslinked using the reversible protein crosslinking reagent, 2-iminothiolane, under conditions shown previously to lead to the formation of dimers between 40S proteins but not higher multimers. The activity of both th...


    pub_type: 杂志文章


    authors: Tolan DR,Hershey JW,Traut RT

    更新日期:1983-07-01 00:00:00

  • A single element in the phosphoenolpyruvate carboxykinase gene mediates thiazolidinedione action specifically in adipocytes.

    abstract::Phosphoenolpyruvate carboxykinase (PEPCK) is the key enzyme in glyceroneogenesis, an important metabolic pathway that functions to restrain the release of non-esterified fatty acids (NEFAs) from adipocytes. The antidiabetic drugs known as thiazolidinediones (TZDs) are thought to achieve some of their benefits by lower...


    pub_type: 杂志文章


    authors: Glorian M,Duplus E,Beale EG,Scott DK,Granner DK,Forest C

    更新日期:2001-10-01 00:00:00

  • Complete sequence of one of the mRNAs coding for the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris.

    abstract::The combination of cDNA and RNA sequencing techniques has enabled determination of the complete sequence of one of the mRNAs coding for the precursor of the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris. In this 898-nucleotide-long mRNA, 540 nucleotides code for the entire 180-amino-acid-l...


    pub_type: 杂志文章


    authors: Pinck M,Guilley E,Durr A,Hoff M,Pinck L,Fleck J

    更新日期:1984-07-01 00:00:00

  • Identification of new bone morphogenetic protein-related members in invertebrates.

    abstract::Although believed to be widely distributed among the animal kingdom, transforming growth factor-beta (TGF-beta) superfamily members have mainly been characterized in vertebrate and in invertebrate model organisms such as Drosophila and C. elegans. To characterize such new factors in invertebrates, a PCR screen was per...


    pub_type: 杂志文章


    authors: Lelong C,Mathieu M,Favrel P

    更新日期:2001-05-01 00:00:00

  • Epigenetics in asthma and COPD.

    abstract::Epigenetic mechanisms are likely to play a role in many complex diseases, the extent of which we only beginning to understand. COPD and asthma are two respiratory diseases subject to strong environmental influences depending on underlying genetic susceptibility. Epigenetic mechanisms such as DNA methylation, histone m...


    pub_type: 杂志文章,评审


    authors: Kabesch M,Adcock IM

    更新日期:2012-11-01 00:00:00

  • Structural studies of glycans isolated from rat plasma hemopexin.

    abstract::After exhaustive pronase digestion, purification by gel filtration and affinity chromatography on concanavalin A, three glycopeptide fractions were obtained from rat hemopexin. Two fractions (I and II) were concanavalin A non-reactive and one (III) was concanavalin A reactive. On the basis of carbohydrate composition,...


    pub_type: 杂志文章


    authors: Bernard N,Lombart C,Strecker G,Montreuil J,Van Halbeek H,Vliegenthart JF

    更新日期:1983-03-01 00:00:00

  • Molecular cloning and characterization of neutral ceramidase homologue from the red flour beetle, Tribolium castaneum.

    abstract::Ceramidase plays an important role in regulating the metabolism of sphingolipids, such as ceramide, sphingosine (SPH), and sphingosine-1-phosphate (S1P), by controlling the hydrolysis of ceramide. Here we report the cloning and biochemical characterization of a neutral ceramidase from the red flour beetle Tribolium ca...


    pub_type: 杂志文章


    authors: Zhou Y,Lin XW,Yang Q,Zhang YR,Yuan JQ,Lin XD,Xu R,Cheng J,Mao C,Zhu ZR

    更新日期:2011-07-01 00:00:00

  • Aptamers in Bordeaux 2017: An exceptional "millésime".

    abstract::About 150 participants attended the symposium organised at the Palais de la Bourse in Bordeaux, France on September 22-23, 2017. Thirty speakers from all over the world delivered lectures covering selection processes, aptamer chemistry and innovative applications of these powerful tools that display major advantages o...


    pub_type: 杂志文章


    authors: Toulmé JJ,Azéma L,Darfeuille F,Dausse E,Durand G,Paurelle O

    更新日期:2018-02-01 00:00:00

  • Regulation of translation and cell growth by eIF-4E.

    abstract::This review discusses the regulation of a key controlling step in the initiation of protein synthesis, the binding of mRNA to ribosomes. Particular focus is given to the phosphorylation of the cap-binding factor, eIF-4E, and the role of this factor in the regulation of cell growth. ...


    pub_type: 杂志文章,评审


    authors: Sonenberg N

    更新日期:1994-01-01 00:00:00

  • Studies on the effect of bile salt and colipase on enzymatic lipolysis. Improved method for the determination of pancreatic lipase and colipase.

    abstract::The rate of hydrolysis of long chain triglycerides by pure bovine pancreatic lipase has been determined in the presence of variable amounts of bile salts and colipase. Cofactor-free lipase is strongly inhibited by sodium taurodesoxycholate and by mixed bovine bile salts at concentrations higher than the critical micel...


    pub_type: 杂志文章


    authors: Rathelot J,Julien R,Canioni P,Coeroli C,Sarda L

    更新日期:1975-01-01 00:00:00

  • Recognition of cell surface acceptors by two human alpha-2,6-sialyltransferases produced in CHO cells.

    abstract::The action of sialyltransferases (STs) on cell surface glycoconjugates is a key process in shaping cell phenotype in a variety of cells mostly involved in migratory and adhesive pathways. The factors determining cell-specific pattern of glycosylation are so far poorly understood. Most STs are resident proteins of the ...


    pub_type: 杂志文章


    authors: Donadio S,Dubois C,Fichant G,Roybon L,Guillemot JC,Breton C,Ronin C

    更新日期:2003-03-01 00:00:00

  • Efficient adenoviral transduction of 3T3-F442A preadipocytes without affecting adipocyte differentiation.

    abstract::The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene ...


    pub_type: 杂志文章


    authors: Béréziat V,Moritz S,Klonjkowski B,Decaudain A,Auclair M,Eloit M,Capeau J,Vigouroux C

    更新日期:2005-11-01 00:00:00

  • Serotonin in retina.

    abstract::The expression of serotonin (5-HT) in the retina was first reported in the sixties. The detection of vesicular monoamine transporter and serotonin receptors in several retinal cells confirm that 5-HT is playing a neuromodulatory role in this structure. Whereas signaling pathways activated by 5-HT receptor binding has ...


    pub_type: 杂志文章,评审


    authors: Masson J

    更新日期:2019-06-01 00:00:00

  • Development and preclinical evaluation of a new F(ab')₂ antitoxin against botulinum neurotoxin serotype A.

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    pub_type: 杂志文章


    authors: Yu YZ,Zhang SM,Wang WB,Du Y,Zhu HQ,Wang RL,Zhou XW,Lin JB,Wang S,Yu WY,Huang PT,Sun ZW

    更新日期:2010-10-01 00:00:00

  • The mechanics of DNA loops bridged by proteins unveiled by single-molecule experiments.

    abstract::Protein-induced DNA bridging and looping is a common mechanism for various and essential processes in bacterial chromosomes. This mechanism is preserved despite the very different bacterial conditions and their expected influence on the thermodynamic and kinetic characteristics of the bridge formation and stability. O...


    pub_type: 杂志文章,评审


    authors: Tardin C

    更新日期:2017-11-01 00:00:00

  • The yeast ATP synthase subunit 4: structure and function.

    abstract::The structure of ATP synthase subunit 4 was determined by using the oligonucleotide probe procedure. This subunit is the fourth polypeptide of the complex when classifying subunits in order of decreasing molecular mass. Its relative molecular mass is 25 kDa. The ATP4 gene was isolated and sequenced. The nucleotide seq...


    pub_type: 杂志文章


    authors: Velours J,Arselin G,Paul MF,Galante M,Durrens P,Aigle M,Guerin B

    更新日期:1989-08-01 00:00:00

  • BRD4 contributes to high-glucose-induced podocyte injury by modulating Keap1/Nrf2/ARE signaling.

    abstract::High glucose (HG)-induced podocyte injury contributes to the pathogenesis of diabetic nephropathy, a severe complication of diabetes. Bromodomain-containing protein 4 (BRD4) has emerged as a critical regulator for cell injury. However, whether BRD4 participates in HG-induced podocyte injury remains unclear. In this st...


    pub_type: 杂志文章


    authors: Zuo H,Wang S,Feng J,Liu X

    更新日期:2019-10-01 00:00:00

  • Molecular property-binding affinity relationship of flavonoids for common rat plasma proteins in vitro.

    abstract::The structural difference of flavonoids strongly affects the binding process with plasma proteins. This work in here mainly concerns about the molecular property-binding affinity relationship of dietary flavonoids for common rat plasma proteins (CRPP). The magnitudes of binding constants between flavonoids and CRPP we...


    pub_type: 杂志文章


    authors: Xiao J,Cao H,Chen T,Yang F,Liu C,Xu X

    更新日期:2011-02-01 00:00:00

  • Exploiting the unique features of Zika and Dengue proteases for inhibitor design.

    abstract::Zika and Dengue viruses have attracted substantial attention from researchers in light of recent outbreaks of Dengue fever and increases in cases of congenital microcephaly in areas with Zika incidence. This review summarizes the current state of knowledge about Zika and Dengue proteases. These enzymes have several in...


    pub_type: 杂志文章,评审


    authors: Majerová T,Novotný P,Krýsová E,Konvalinka J

    更新日期:2019-11-01 00:00:00

  • Peptide synthesis by recombinant Fasciola hepatica cathepsin L1.

    abstract::Synthesis of the tripeptide Z-Phe-Arg-SerNH2 has been accomplished by a recombinant cysteine protease, cathepsin L1 from liver fluke (Fasciola hepatica), using Z-Phe-Arg-OMe as acyl acceptor and SerNH2 as nucleophile in 0.1 M ammonium acetate pH 9.0-12.5% v/v acetonitrile at 37 degrees C. LC-MS detection indicated tri...


    pub_type: 杂志文章


    authors: Ruth DM,McMahon G,O'Fágáin C

    更新日期:2006-01-01 00:00:00

  • Natural mutations lead to enhanced proteasomal degradation of human Ncb5or, a novel flavoheme reductase.

    abstract::NADH cytochrome b5 oxidoreductase (Ncb5or) protects β-cells against oxidative stress and lipotoxicity. The predominant phenotype of lean Ncb5or-null mouse is insulin-dependent diabetes due to β-cell death. This suggests the putative role of NCB5OR polymorphism in human diabetes. Therefore, we aimed to investigate the ...


    pub_type: 杂志文章


    authors: Kálmán FS,Lizák B,Nagy SK,Mészáros T,Zámbó V,Mandl J,Csala M,Kereszturi E

    更新日期:2013-07-01 00:00:00

  • Mixed micelle properties and intestinal cholesterol uptake.

    abstract::The solubilizing powers of taurocholate, taurochenodeoxycholate and tauroursodeoxycholate for monoolein and cholesterol, and the size of the bile salt-monoolein-cholesterol micelles have been determined. For the three bile salt species, the micellar size depends on the saturation with monoolein. As a result, for a giv...


    pub_type: 杂志文章


    authors: Montet JC,Lindheimer M,Reynier MO,Crotte C,Bontemps R,Gerolami A

    更新日期:1982-04-01 00:00:00

  • From the pigments of the actinomycetes to third generation antitumor anthracyclines.

    abstract::After the assessment of the antitumor activity of the anthracycline pigments, the S peucetius group of metabolites was discovered and eventually doxorubicin, a major anticancer agent of established clinical usefulness was developed in the early seventies. A second generation of compounds followed, represented mainly b...


    pub_type: 杂志文章,评审


    authors: Arcamone FM

    更新日期:1998-03-01 00:00:00

  • Born to run; the story of the PEPCK-Cmus mouse.

    abstract::In order to study the role of the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) (EC (PEPCK-C) in skeletal muscle, PEPCK-Cmus mice were created by introducing the cDNA for the enzyme, linked to the human alpha-skeletal actin gene promoter, into their germ line. Two founder lines generated by this ...


    pub_type: 杂志文章,评审


    authors: Hanson RW,Hakimi P

    更新日期:2008-06-01 00:00:00

  • Comparison of physico-chemical characteristics of four laccases from different basidiomycetes.

    abstract::New strains of basidiomycetes producing extracellular laccases (Trametes ochracea 92-78, and Trametes hirsuta 56) have been found by screening of isolates of Trametes fungi. The laccases from T. hirsuta 56 and T. ochracea 92-78 as well as two laccases from previously found and described strains of basidiomycetes, name...


    pub_type: 杂志文章


    authors: Shleev SV,Morozova OV,Nikitina OV,Gorshina ES,Rusinova TV,Serezhenkov VA,Burbaev DS,Gazaryan IG,Yaropolov AI

    更新日期:2004-09-01 00:00:00

  • OURCIN: a tool to build expert systems.

    abstract::OURCIN is a tool to build expert systems, which was developed by a joint team from INRIA and SEMA. We emphasized developing on this system the ergonomic features which make Expert Systems a comfortable and interesting approach for knowledge engineering. ...


    pub_type: 杂志文章


    authors: Quinqueton J

    更新日期:1985-05-01 00:00:00

  • Amino acid sequences of cytochromes c2 and c' from the moderately halophilic purple phototrophic bacterium Rhodospirillum salexigens.

    abstract::Rhodospirillum salexigens is a moderately halophilic purple phototrophic bacterium which grows optimally in 8% NaCl. The amino acid sequences of the two principal soluble cytochromes c have been determined. One of these is a cytochrome c2, similar in size to mitochondrial cytochrome c. While clearly of the same sequen...


    pub_type: 杂志文章


    authors: Ambler RP,Daniel M,Meyer TE,Kamen MD

    更新日期:1994-01-01 00:00:00

  • Identification of a new subtype of dipeptidyl peptidase 11 and a third group of the S46-family members specifically present in the genus Bacteroides.

    abstract::Peptidase family S46 consists of two types of dipeptidyl-peptidases (DPPs), DPP7 and DPP11, which liberate dipeptides from the N-termini of polypeptides along with the penultimate hydrophobic and acidic residues, respectively. Their specificities are primarily defined by a single amino acid residue, Gly673 in DPP7 and...


    pub_type: 杂志文章


    authors: Nemoto TK,Bezerra GA,Ono T,Nishimata H,Fujiwara T,Ohara-Nemoto Y

    更新日期:2018-04-01 00:00:00