Reciprocal effects of 2-fluoro-2-deoxy-D-glucose and glucose on their metabolism in Saccharomyces cerevisiae studied by multi-nuclear NMR spectroscopy.


:The effects of various concentrations of 2-fluoro-2-deoxy-D-glucose (FDG) on the aerobic metabolism of glucose and the reciprocal effect of glucose on the metabolism of FDG in glucose-grown repressed Saccharomyces cerevisiae cells were studied at 30 degrees C in a standard pyrophosphate medium containing 5 x 10(7) cells/ml by 1H-, 19F-, 31P-NMR and biochemical techniques. The glucose consumption rate is reduced by about 57% and 71% in the presence of 5 mM FDG and 10 mM FDG respectively. Under the same conditions, the ethanol production rate also decreases about 54% and 68%, respectively. When FDG is the unique carbon source, the alpha- and beta-anomers of 2-fluoro-2-deoxy-D-glucose-6-phosphate (FDG6P) and a much smaller quantity of 2-fluoro-2-deoxy-gluconic acid (FDGA) were observed. The quantities of alpha- and beta-FDG6P reach their maximum values within 1 h of incubation and then decrease continuously. In contrast, Glc favors the consumption of FDG and the synthesis of FDG6P and uridine-5'-diphosphate fluorodeoxy-glucose (UDP-FDG). In the presence of Glc, FDG6P reaches a plateau after 1 h or 2 h of incubation while UDP-FDG increases regularly with time. Apart from trehalose, no other disaccharide such as fluoro-dideoxy-trehalose (FDG-FDG) or fluoro-deoxy-trehalose (FDG-Glc) were observed. Thus, in contrast to UDP-Glc, UDP-DG, Glc6P and DG6P, UDP-FDG and FDG6P are not good substrates for trehalose-6-P synthetase.(ABSTRACT TRUNCATED AT 250 WORDS)






Tran-Dinh S,Courtois A,Wietzerbin J,Bouet F,Herve M




Has Abstract


1995-01-01 00:00:00














  • Metal binding and antioxidant properties of chimeric tri- and tetra-domained metallothioneins.

    abstract::An unusual tri-domained (alpha-beta-beta) natural oyster metallothionein (MT) is known, and non-oxidative MT dimers occur in vivo in mollusk species and in mammals. To assess the respective role of the MT domains, two chimeric MTs were constructed: a tetra-domained oyster MT corresponding to the alpha-beta-alpha-beta ...


    pub_type: 杂志文章


    authors: Moreau JL,Baudrimont M,Carrier P,Peltier G,Bourdineaud JP

    更新日期:2008-05-01 00:00:00

  • An active site mutant of Escherichia coli cyclopropane fatty acid synthase forms new non-natural fatty acids providing insights on the mechanism of the enzymatic reaction.

    abstract::We have produced and purified an active site mutant of the Escherichia coli cyclopropane fatty acid synthase (CFAS) by replacing the strictly conserved G236 within cyclopropane synthases, by a glutamate residue, which corresponds to E146 of the homologous mycolic acid methyltransferase, Hma, producing hydroxymethyl my...


    pub_type: 杂志文章


    authors: E G,Drujon T,Correia I,Ploux O,Guianvarc'h D

    更新日期:2013-12-01 00:00:00

  • Crystal structure of dihydroorotate dehydrogenase from Leishmania major.

    abstract::Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine biosynthetic pathway and has been exploited as the target for therapy against proliferative and parasitic diseases. In this study, we report the crystal structures of DHODH from Leishmania major, the species of Leishmania associated wi...


    pub_type: 杂志文章


    authors: Cordeiro AT,Feliciano PR,Pinheiro MP,Nonato MC

    更新日期:2012-08-01 00:00:00

  • Running up that hill: How to create cellular lipid gradients by lipid counter-flows.

    abstract::Lipids are unevenly distributed within eukaryotic cells, allowing various processes to work in an optimized membrane environment. Rather than following vesicular trafficking pathways, certain lipids are transported by lipid transfer proteins (LTPs), some at sites of close apposition of two organelles called membrane c...


    pub_type: 杂志文章,评审


    authors: Moser von Filseck J,Drin G

    更新日期:2016-11-01 00:00:00

  • Hypothesis: membrane domains and hyperstructures control bacterial division.

    abstract::The mechanism responsible for creating the division site in the right place at the right time in bacteria is unknown. It has been attributed to the formation of proteolipid domains in the cytoplasmic membrane surrounding the nucleoids. We interpret the growing evidence for this hypothesis by invoking hyperstructures, ...


    pub_type: 杂志文章,评审


    authors: Norris V,Fishov I

    更新日期:2001-01-01 00:00:00

  • A thioredoxin reductase and/or thioredoxin system-based mechanism for antioxidant effects of ambroxol.

    abstract::Long-term treatment with ambroxol (ABX), a bronchial expectorant, was found to prevent acute exacerbation of chronic obstructive pulmonary disease (AECOPD). The underlying mechanism remains unclear. To address this, we have investigated the effect of ABX on critical antioxidant proteins thioredoxin (Trx) and thioredox...


    pub_type: 杂志文章


    authors: Huang J,Xu J,Tian L,Zhong L

    更新日期:2014-02-01 00:00:00

  • The nutraceutical potential of omega-3 alpha-linolenic acid in reducing the consequences of stroke.

    abstract::Stroke is a worldwide major cause of mortality and morbidity. Preclinical studies have identified over 1000 molecules with brain-protective properties. More than 200 clinical trials have evaluated neuroprotective candidates for ischemic stroke yet, to date almost all failed, leading to a re-analysis of treatment strat...


    pub_type: 杂志文章,评审


    authors: Blondeau N

    更新日期:2016-01-01 00:00:00

  • Purification and characterization of extracellular pectate lyase from Bacillus subtilis.

    abstract::Bacillus subtilis strain SO113 secretes a pectate lyase which is produced during the exponential death phase of growth, just before sporulation. This extracellular pectate lyase, which produces unsaturated products from polygalacturonate, was purified 35-fold from the culture supernatant of Bacillus subtilis by a CM S...


    pub_type: 杂志文章


    authors: Nasser W,Chalet F,Robert-Baudouy J

    更新日期:1990-09-01 00:00:00

  • Effects of folic acid withdrawal on transcriptomic profiles in murine triple-negative breast cancer cell lines.

    abstract::We have previously shown that withdrawal of folic acid led to metabolic reprogramming and a less aggressive phenotype in a mouse cell model of triple-negative breast cancer (TNBC). Herein, we evaluate the effects of folic acid withdrawal on transcriptomic profiles in these cells. Murine cell lines were originally deri...


    pub_type: 杂志文章


    authors: Kok DE,O'Flanagan CH,Coleman MF,Ashkavand Z,Hursting SD,Krupenko SA

    更新日期:2020-06-01 00:00:00

  • Resonance Raman spectroscopy of c-type cytochromes.

    abstract::This paper provides an overview of the usefulness of the resonance Raman (RR) spectroscopy in the determination of the structural and electronic properties of heme(s) included in c-type cytochromes. It reviews the mode assignments presently available for heme c and includes recent RR data on the most important subclas...


    pub_type: 杂志文章,评审


    authors: Desbois A

    更新日期:1994-01-01 00:00:00

  • Characterisation of a mannose-binding C-type lectin from Oxyuranus scutellatus snake venom.

    abstract::C-type lectins are calcium-dependent sugar binding proteins and are distributed ubiquitously amongst vertebrate organisms. As part of a wider study on Australian snake venom components, we have identified and characterised a C-type lectin from the venom of Oxyuranus scutellatus (Australian coastal taipan) with mannose...


    pub_type: 杂志文章


    authors: Earl ST,Robson J,Trabi M,de Jersey J,Masci PP,Lavin MF

    更新日期:2011-03-01 00:00:00

  • Functional role of annexins in zebrafish caudal fin regeneration - A gene knockdown approach in regenerating tissue.

    abstract::Regeneration is an adaptive phenomenon with wide biological implications spread heterogeneously in almost all the organism including human beings. The ability of regeneration varies from species to species for its complexity. Epimorphic regeneration of zebrafish caudal fin tissue is the most widely studied regeneratio...


    pub_type: 杂志文章


    authors: Quoseena M,Vuppaladadium S,Hussain S,Banu S,Bharathi S,Idris MM

    更新日期:2020-08-01 00:00:00

  • Regulation of CIRL-1 proteolysis and trafficking.

    abstract::Calcium-independent receptor of alpha-latrotoxin (CIRL-1) is an adhesion G protein-coupled receptor implicated in the regulation of exocytosis. CIRL-1 biosynthesis involves constitutive proteolytic processing that takes place in the endoplasmic reticulum, requires the receptor's GPS domain, and yields heterologous two...


    pub_type: 杂志文章


    authors: Deyev IE,Petrenko AG

    更新日期:2010-04-01 00:00:00

  • Thiolation of low-density lipoproteins and their interaction with L2C leukemic lymphocytes.

    abstract::We present here, a new method for coupling sulfhydryl groups (SH) to low-density lipoprotein (LDL) surface. This method uses homocysteine thiolactone (HCTL) which reacts with lysine residues in a very mild manner, and permits the selection of the number of SH bound per LDL. Under our experimental conditions (8 SH/LDL)...


    pub_type: 杂志文章


    authors: Vidal M,Sainte-Marie J,Philippot J,Bienvenue A

    更新日期:1986-05-01 00:00:00

  • Inhibitor of differentiation 4 (ID4) acts as an inhibitor of ID-1, -2 and -3 and promotes basic helix loop helix (bHLH) E47 DNA binding and transcriptional activity.

    abstract::The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed...


    pub_type: 杂志文章


    authors: Sharma P,Chinaranagari S,Chaudhary J

    更新日期:2015-05-01 00:00:00

  • Human erythrocyte phosphoglucomutase: comparison of the kinetic properties of PGM1 and PGM2 isoenzymes.

    abstract::Kinetic properties of PGM1 and PGM2 phosphoglucomutase "primary" isoenzymes from human erythrocytes were studied. The two enzyme forms share a "ping-pong" kinetic mechanism and show similar Km for substrate (glucose 1-P) and cofactor (glucose 1,6-P2). Micromolar concentrations of fructose 1,6-P2 and glycerate 2,3-P2 i...


    pub_type: 杂志文章


    authors: Ninfali P,Accorsi A,Palma F,Fazi A,Piatti E,Chiarantini L,Fornaini G

    更新日期:1984-09-01 00:00:00

  • Effect of liver plasma membrane fluidity on endogenous phospholipase A2 activity.

    abstract::Investigations have been carried out on the influence of the phospholipid composition and the physicochemical properties of rat liver plasma membranes on the endogenous activity of membrane-bound phospholipase A2. The membrane phospholipid composition was modified by the incorporation of different phospholipids in the...


    pub_type: 杂志文章


    authors: Petkova DH,Momchilova-Pankova AB,Koumanov KS

    更新日期:1987-11-01 00:00:00

  • A C-terminal hydrophobic, solvent-protected core and a flexible N-terminus are potentially required for human papillomavirus 18 E7 protein functionality.

    abstract::The oncogenic potential of the high-risk human papillomavirus (HPV) relies on the expression of genes specifying the E7 and E6 proteins. To investigate further the variation in oligomeric structure that has been reported for different E7 proteins, an HPV-18 E7 cloned from a Hispanic woman with cervical intraepithelial...


    pub_type: 杂志文章


    authors: Liu S,Tian Y,Greenaway FT,Sun MZ

    更新日期:2010-07-01 00:00:00

  • Mutations in ribosomal proteins L7/L12 perturb EF-G and EF-Tu functions.

    abstract::In vitro cycling rates of E. coli ribosomes and of elongation factors EF-Tu and EF-G have been obtained and these are compatible with translation rates in vivo. We show that the rate of translocation is faster than 50 s-1 and therefore that the EF-G function is not a rate limiting step in protein synthesis. The in viv...


    pub_type: 杂志文章


    authors: Bilgin N,Kirsebom LA,Ehrenberg M,Kurland CG

    更新日期:1988-05-01 00:00:00

  • Assembly of chloroplast cytochromes b and c.

    abstract::The synthesis of holocytochromes in plastids is a catalyzed process. Several proteins, including plastid CcsA, Ccs1, possibly CcdA and a thioredoxin, plus at least two additional Ccs factors, are required in sub-stoichiometric amounts for the conversion of apocytochromes f and c(6) to their respective holoforms. CcsA,...


    pub_type: 杂志文章,评审


    authors: Nakamoto SS,Hamel P,Merchant S

    更新日期:2000-06-01 00:00:00

  • The DNA-binding protein HBsu is essential for normal growth and development in Bacillus subtilis.

    abstract::The hbs operon of Bacillus subtilis comprises a single gene which is localized between the spoIVA and mtrA open reading frames, and is situated at 204 degrees of the standard map of B subtilis. Expression of hbs is initiated from two distinct promoters called P1 and P2. The transcription initiation sites have been map...


    pub_type: 杂志文章


    authors: Micka B,Marahiel MA

    更新日期:1992-07-01 00:00:00

  • Use of crosslinking for revealing the DNA phosphate groups forming specific contacts with the E. coli Fpg protein.

    abstract::Specific contacts between DNA phosphate groups and positively charged nucleophilic amino acids from the Escherichia coli Fpg protein play a significant role in DNA-Fpg protein interaction. In order to identify these phosphate groups the chemical crosslinking procedure was carried out. The probing of the Fpg protein ac...


    pub_type: 杂志文章


    authors: Kuznetsova S,Rykhlevskaya A,Taranenko M,Sidorkina O,Oretskaya T,Laval J

    更新日期:2003-05-01 00:00:00

  • A potyvirus-based gene vector allows producing active human S-COMT and animal GFP, but not human sorcin, in vector-infected plants.

    abstract::Potato virus A (PVA), a potyvirus with a (+)ssRNA genome translated to a large polyprotein, was engineered and used as a gene vector for expression of heterologous proteins in plants. Foreign genes including jellyfish GFP (Aequorea victoria) encoding the green fluorescent protein (GFP, 27 kDa) and the genes of human o...


    pub_type: 杂志文章


    authors: Kelloniemi J,Mäkinen K,Valkonen JP

    更新日期:2006-05-01 00:00:00

  • [Purification and characterization of an extracellular protease from Staphylococcus aureus inhibited by EDTA].

    abstract::A neutral protease, produced by Staphylococcus aureus, strain V8, was purified by precipitation with ammonium sulfate and acetone, and by chromatography on D.E.A.E.-cellulose. The purified protein is homogeneous by acrylamide gel electrophoresis. The molecular weight of the enzyme is estimated to be 28.000 daltons by ...


    pub_type: 杂志文章


    authors: Saheb SA

    更新日期:1976-01-01 00:00:00

  • A region in the C-terminal domain of ribosomal protein SA required for binding of SA to the human 40S ribosomal subunit.

    abstract::The human ribosomal protein SA, known also as a precursor of the cell-surface laminin receptor, LAMR, is a protein of the 40S ribosomal subunit. It is homologous to eubacterial ribosomal protein S2p, but has a eukaryote-specific C-terminal domain (CTD) that is responsible in LAMR for the binding of laminin as well as ...


    pub_type: 杂志文章


    authors: Malygin AA,Babaylova ES,Loktev VB,Karpova GG

    更新日期:2011-03-01 00:00:00

  • Cyanidin-3-glucoside induces mesenchymal to epithelial transition via activating Sirt1 expression in triple negative breast cancer cells.

    abstract::Triple-negative breast cancer (TNBC) is a heterogeneous group of breast cancer with one common feature: distinctly metastatic nature with higher rate of relapse and shorter survival compared with other subtypes of breast cancer. The epithelial to mesenchymal transition (EMT) is highly associated with cancer metastasis...


    pub_type: 杂志文章


    authors: Liang L,Liu X,He J,Shao Y,Liu J,Wang Z,Xia L,Han T,Wu P

    更新日期:2019-07-01 00:00:00

  • Structure-function relationships in mammalian histidine-proline-rich glycoprotein.

    abstract::Histidine-proline-rich glycoprotein (HPRG), or histidine-rich glycoprotein (HRG), is a serum protein that is synthesized in the liver and is actively internalised by different cells, including skeletal muscle. The multidomain arrangement of HPRG comprises two modules at the N-terminus that are homologous to cystatin b...


    pub_type: 杂志文章,评审


    authors: Ronca F,Raggi A

    更新日期:2015-11-01 00:00:00

  • 'Partitagin' a hemorrhagic metalloprotease from Hippasa partita spider venom: role in tissue necrosis.

    abstract::The poisonous bite by Hippasa partita, a funnel web spider from the Indian subcontinent has been demonstrated to give rise to severe dermo- and myonecrosis. In this work a hemorrhagic metalloprotease, Partitagin was purified from H. partita venom by successive chromatography on Sephadex G-100, DEAE Sephadex A-50 and B...


    pub_type: 杂志文章


    authors: Nagaraju S,Girish KS,Fox JW,Kemparaju K

    更新日期:2007-11-01 00:00:00

  • The yeast ATP synthase subunit 4: structure and function.

    abstract::The structure of ATP synthase subunit 4 was determined by using the oligonucleotide probe procedure. This subunit is the fourth polypeptide of the complex when classifying subunits in order of decreasing molecular mass. Its relative molecular mass is 25 kDa. The ATP4 gene was isolated and sequenced. The nucleotide seq...


    pub_type: 杂志文章


    authors: Velours J,Arselin G,Paul MF,Galante M,Durrens P,Aigle M,Guerin B

    更新日期:1989-08-01 00:00:00

  • DNA binding thermodynamics and site stoichiometry as a function of polyamide size.

    abstract::The interactions of 6-8 ring hairpin polyamides (PAs) with the minor groove of DNA have been investigated extensively. More recent studies of large antiviral PAs (14-20 rings) active against small DNA tumor viruses lead to questions regarding the extent to which the DNA binding behaviors of the well studied, smaller P...


    pub_type: 杂志文章


    authors: Song Y,Niederschulte J,Bales KN,Park AH,Bashkin JK,Dupureur CM

    更新日期:2019-10-01 00:00:00