Abstract:
:Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards β-brominated alkanes. Moreover, we identified other putative haloalkane dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties.
journal_name
Biochimiejournal_title
Biochimieauthors
Fortova A,Sebestova E,Stepankova V,Koudelakova T,Palkova L,Damborsky J,Chaloupkova Rdoi
10.1016/j.biochi.2013.07.025subject
Has Abstractpub_date
2013-11-01 00:00:00pages
2091-6issue
11eissn
0300-9084issn
1638-6183pii
S0300-9084(13)00248-4journal_volume
95pub_type
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