Abstract:
:A neutral protease, produced by Staphylococcus aureus, strain V8, was purified by precipitation with ammonium sulfate and acetone, and by chromatography on D.E.A.E.-cellulose. The purified protein is homogeneous by acrylamide gel electrophoresis. The molecular weight of the enzyme is estimated to be 28.000 daltons by gel filtration and 27.400 daltons by sedimentation equilibrium study. The amino acid composition indicates 251 residues and the absence of sulphydryl groups. Arginine is the NH2-terminal amino acid, while the COOH-terminal sequence of amino acids is Ala-Gly-Leu-Gl-Val. The protease exhibits a maximum proteolytic activity on casein at pH 7,0, and its activity is increased by 30 per cent on the addition of calcium ions. E.D.T.A. and E.G.T.A. completely inhibit the protease while the reagents of serine enzymes are without effect. This enzyme, which contains mainly calcium in its molecule, hydrolyses the peptide bonds, of the insulin B-chain, in which the amino groups of hydrophobic amino acids are involved. Unlike the other metalloproteases, no hydrolysis is observed for the peptide bond Phe(24)-Phe(25) while the peptide bond Phe(1)-Val(2) is attacked.
journal_name
Biochimiejournal_title
Biochimieauthors
Saheb SAdoi
10.1016/s0300-9084(76)80310-0subject
Has Abstractpub_date
1976-01-01 00:00:00pages
793-804issue
7eissn
0300-9084issn
1638-6183pii
S0300-9084(76)80310-0journal_volume
58pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90175-g
更新日期:1990-01-01 00:00:00
abstract::Human telomeres are intrinsically dynamic structures, with multiple biological processes operating to generate substantial length heterogeneity. Processes that operate specifically at the terminus, that include the end-replication problem coupled with C-strand resection, result in gradual telomere erosion with ongoing...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.08.003
更新日期:2008-01-01 00:00:00
abstract::Glycosphingolipids and sphingolipids of cellular plasma membranes (PMs) reach luminal intra-lysosomal vesicles (LVs) for degradation mainly by pathways of endocytosis. After a sorting and maturation process (e.g. degradation of sphingomyelin (SM) and secretion of cholesterol), sphingolipids of the LVs are digested by ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2016.05.004
更新日期:2016-11-01 00:00:00
abstract::Hypertension is the leading cause of preventable mortality worldwide, contributing to over 9 million deaths per annum, predominantly owing to cardiovascular disease. The association of obesity, physical inactivity and alcohol with elevated blood pressure (BP) is firmly established. Weight loss or other dietary strateg...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2020.03.016
更新日期:2020-06-01 00:00:00
abstract::GcvB is a non-coding RNA that regulates oppA mRNA in different bacterial species by binding a GcvB GU-rich region named R1 to oppA mRNA. A secondary putative interaction site (PS1) was identified in this study that is able to form a second nearly perfect 10 base-pair duplex between these two RNAs in Escherichia coli. ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.06.020
更新日期:2010-10-01 00:00:00
abstract::Long-term exposure to high doses of caffeine affects several aspects of nitrogen metabolism, such as purine, pyrimidine and urea synthesis. However, little is known about the mechanisms of these changes and if they occur at shorter term. We have studied in isolated hepatocytes: 1) the in vitro effect of high doses of ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90014-4
更新日期:1988-10-01 00:00:00
abstract::Numerous studies have reported the beneficial effects of antioxidants in human diseases. Among their biological effects, a majority of antioxidants scavenge reactive radicals in the body, thereby reducing oxidative stress that is associated with the pathogenesis of many diseases. Antioxidant dendrimers are a new class...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.02.001
更新日期:2015-04-01 00:00:00
abstract::Many human conditions, including neurological diseases, atherosclerosis, cancer, diabetic complications and aging, are thought to be associated with oxidative stress (OS). The development of reliable and informative markers for the characterization of OS in humans is thus highly important. Various endogenous markers a...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.03.005
更新日期:2013-03-01 00:00:00
abstract::Oligonucleotides are multifunctional molecules which can interfere with gene expression by different mechanism such as antisense, RNA interference, ribozymes, etc. For most in vivo diagnostic and therapeutic applications, oligonucleotides need to be delivered to the intracellular compartment of a specific organ, a dif...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2005.04.013
更新日期:2005-09-01 00:00:00
abstract::Two forms of baker's yease valyl-tRNA synthetase have been purified to apparent homogeneity by classical methods. It was demonstrated that one of the two forms of the enzyme originates from the other by proteolysis, the respective amounts of each form depending on the physiological state of the yeast. The species main...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80579-2
更新日期:1975-01-01 00:00:00
abstract::In order to confirm the presence of a phosphoenolpyruvate (PEP)-dependent sugar-phosphotransferase system (PTS) in the mollicute Acholeplasma florum we studied the ability of cell free extracts of this organism to phosphorylate glucose and/or fructose in the presence of PEP. We also cloned and sequenced a DNA fragment...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90098-d
更新日期:1993-01-01 00:00:00
abstract::When colonizing the digestive tract of mono-associated rats, Ruminococcus gnavus E1 - a bacterium isolated from human faeces - produced a trypsin-dependent anti-Clostridium perfringens substance collectively named Ruminococcin C (RumC). RumC was isolated from the caecal contents of E1-monocontaminated rats and found t...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.05.001
更新日期:2011-09-01 00:00:00
abstract::SR-PSOX can function as a scavenger receptor, a chemokine and an adhesion molecule, and it could be an interesting player in the formation of atherosclerotic lesions. Our previous studies demonstrated that basic amino acid residues in the chemokine domain of SR-PSOX are critical for its functions. In this study the co...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.09.016
更新日期:2013-02-01 00:00:00
abstract::The toxicity of amyloids is a subject under intense scrutiny. Many studies link this toxicity to the existence of various intermediate structures prior to the fiber formation and/or their specific interaction with membranes. Membranes can also be a catalyst of amyloidogenesis and the composition or the charge of membr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.07.011
更新日期:2013-01-01 00:00:00
abstract::Sphingosine is a natural sphingolipid found in membranes of all eukaryotic cells. In addition to its functions in cell signaling, sphingosine has broad-spectrum antimicrobial properties. Sphingosine's role as an antimicrobial is important in tissues such as the skin and respiratory epithelium. Reduction in the normal ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.03.014
更新日期:2017-10-01 00:00:00
abstract::Oligonucleotides that complement Escherichia coli 16S ribosomal RNA residues 685-696 and 694-705 have been synthesized so as to incorporate antibody-recognizable markers: a 3'-terminal residue of N6-delta 2-isopentenyladenosine, a 5'-dinitrophenyl group, or both. Each oligonucleotide is able to bind RNA within the sma...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90132-k
更新日期:1991-07-01 00:00:00
abstract::The antiviral activity of L-nucleoside analogs depends in part on the enantioselectivity of nucleoside kinases which catalyse their monophosphorylation. The substrate properties of human recombinant deoxycytidine kinase (dCK) and human recombinant deoxyguanosine kinase (dGK) with respect to L-adenosine and L-guanosine...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)00331-4
更新日期:1999-11-01 00:00:00
abstract::Specific contacts between DNA phosphate groups and positively charged nucleophilic amino acids from the Escherichia coli Fpg protein play a significant role in DNA-Fpg protein interaction. In order to identify these phosphate groups the chemical crosslinking procedure was carried out. The probing of the Fpg protein ac...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00067-1
更新日期:2003-05-01 00:00:00
abstract::Snake venom from Agkistrodon acutus consists of a number of compounds which may potentially be used as drugs. However, it is hard to obtain enough pure protein for drug development. Recently, we reported expression and purification of a novel recombinant fibrinogenase which was named rFII. Here we reported for the fir...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.10.007
更新日期:2009-02-01 00:00:00
abstract::The Ty1 retrotransposon of the yeast Saccharomyces cerevisiae is a long terminal repeat mobile genetic element that transposes through an RNA intermediate. Initiation of minus-strand and plus-strand DNA synthesis are two critical steps during reverse transcription of the retrotransposon genome. Initiation of minus-str...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(96)80013-7
更新日期:1996-01-01 00:00:00
abstract::Heat shock proteins (Hsps) are involved in several important aspects of the cell proteostasis. Hsp90 interacts with at least a tenth of the cell proteome helping a large number of proteins to fold correctly. Hsp90 function is modulated by several co-chaperones having TPR (tetratricopeptide repeat) domains that allow f...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.12.017
更新日期:2021-01-06 00:00:00
abstract::The subunit composition of the myosin molecule which is built up from 3 pairs of identical polypeptide chains (2 heavy chains and 2 pairs of light chains), gives it the appearance of having symmetric structure. This homodimeric arrangement in the molecule is in fact asymmetric in its construction as a result of the na...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(81)80117-4
更新日期:1981-04-01 00:00:00
abstract::Fluorometry using a substrate DNA labeled with a single fluorophore (6-carboxyfluorescein) at the 3'-end of the processed strand was shown to be a useful tool for monitoring DNA-binding and 3'-processing activities of HIV-1 and PFV integrases (INs). The DNA binding to either of the INs resulted in a fluorescence signa...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.06.009
更新日期:2012-11-01 00:00:00
abstract::Beta-turmerin from turmeric (Curcuma longa) waste grits obtained after extraction of curcumin was purified by successive gel permeation chromatography. Homogeneity of beta-turmerin was confirmed by its movement as single band both in SDS-PAGE and as well as in native (basic) PAGE. The apparent molecular mass is approx...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.06.006
更新日期:2009-09-01 00:00:00
abstract::The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence propert...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90089-j
更新日期:1991-10-01 00:00:00
abstract::Sm and Sm-like (Lsm) proteins are considered as an evolutionary conserved family involved in RNA metabolism in organisms from bacteria and archaea to human. Currently, the function of Sm-like archaeal proteins (SmAP) is not well understood. Here, we report the crystal structures of SmAP proteins from Sulfolobus acidoc...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.05.001
更新日期:2020-08-01 00:00:00
abstract::Thyroid peroxidase (TPO) is the key enzyme involved in thyroid hormone synthesis. Autoantibodies to TPO (TPOAbs) are a hallmark of autoimmune thyroid disease (AITD). Here, we highlight recent progress over several years in understanding TPO biochemistry and function in various pathologies. TPO undergoes complex post-t...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2019.02.003
更新日期:2019-05-01 00:00:00
abstract::A thrombin-like enzyme, named BjussuSP-I, isolated from Bothrops jararacussu snake venom, is an acidic single-chain glycoprotein with M(r)=61,000, pI approximately 3.8 and 6% sugar. BjussuSP-I shows high proteolytic activity upon synthetic substrates, such as S-2238 and S-2288. It also shows procoagulant and kallikrei...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.10.005
更新日期:2008-03-01 00:00:00
abstract::Ceramidase plays an important role in regulating the metabolism of sphingolipids, such as ceramide, sphingosine (SPH), and sphingosine-1-phosphate (S1P), by controlling the hydrolysis of ceramide. Here we report the cloning and biochemical characterization of a neutral ceramidase from the red flour beetle Tribolium ca...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.03.009
更新日期:2011-07-01 00:00:00
abstract::The protein "BCL-2-associated athanogene-1" (BAG-1), which exists in multiple isoforms, promotes cancer cell survival and is overexpressed in many different cancers. As a result, BAG-1-targeted therapy appears to be a promising strategy with which to treat cancer. It has previously been shown that the 5'UTR of the BAG...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.06.013
更新日期:2020-09-01 00:00:00