Abstract：
:Pseudouridine [Ψ] is a frequent base modification in the ribosomal RNA [rRNA] and may be involved in the modulation of the conformational flexibility of rRNA helix-loop structures during protein synthesis. Helix 69 of 23S rRNA contains pseudouridines at the positions 1911, 1915 and 1917 which are formed by the helix 69-specific synthase RluD. The growth defect caused by the lack of RluD can be rescued by mutations in class I release factor RF2, indicating a role for helix 69 pseudouridines in translation termination. We investigated the role of helix 69 pseudouridines in peptide release by release factors RF1 and RF2 in an in vitro system consisting of purified components of the Escherichia coli translation apparatus. Lack of all three pseudouridines in helix 69 compromised the activity of RF2 about 3-fold but did not significantly affect the activity of RF1. Reintroduction of pseudouridines into helix 69 by RluD-treatment restored the activity of RF2 in peptide release. A Ψ-to-C substitution at the 1917 position caused an increase in the dissociation rate of RF1 and RF2 from the postrelease ribosome. Our results indicate that the presence of all three pseudouridines in helix 69 stimulates peptide release by RF2 but has little effect on the activity of RF1. The interactions around the pseudouridine at the 1917 position appear to be most critical for a proper interaction of helix 69 with release factors.
journal_name
Biochimiejournal_title
Biochimieauthors
Kipper K,Sild S,Hetényi C,Remme J,Liiv Adoi
10.1016/j.biochi.2010.12.018subject
Has Abstractpub_date
2011-05-01 00:00:00pages
834-44issue
5eissn
0300-9084issn
1638-6183pii
S0300-9084(11)00013-7journal_volume
93pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Adipose tissue is an endocrine organ that plays an essential role in regulating several metabolic functions through the secretion of biological mediators called "adipokines". Dysregulation of adipokines plays a crucial role in obesity-related diseases. Adiponectin (APN) is the most abundant adipokine accounting for th...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.06.030
更新日期：2012-10-01 00:00:00
abstract：:The eosinophil cationic protein (ECP) is a human antimicrobial protein involved in the host immune defense that belongs to the pancreatic RNase A family. ECP displays a wide range of antipathogen activities. The protein is highly cationic and its bactericidal activity is dependant on both cationic and hydrophobic surf...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.10.005
更新日期：2011-02-01 00:00:00
abstract：:New strains of basidiomycetes producing extracellular laccases (Trametes ochracea 92-78, and Trametes hirsuta 56) have been found by screening of isolates of Trametes fungi. The laccases from T. hirsuta 56 and T. ochracea 92-78 as well as two laccases from previously found and described strains of basidiomycetes, name...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2004.08.005
更新日期：2004-09-01 00:00:00
abstract：:The effects of thermal injury (72 h post-injury), 72 h-partial (20% less food) or full starvation on the regulation of phosphofructokinase in the mucosa of rat small intestine were studied. Thermal injury and 72 h-partial or full starvation decreased the activity ratio v0.5/V, but the ratios obtained for thermally inj...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90273-2
更新日期：1987-01-01 00:00:00
abstract：:The eukaryotic ribosomal protein S15 is a key component of the decoding site in contrast to its prokaryotic counterpart, S19p, which is located away from the mRNA binding track on the ribosome. Here, we determined the oligopeptide of S15 neighboring the A site mRNA codon on the human 80S ribosome with the use of mRNA ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.02.031
更新日期：2010-07-01 00:00:00
abstract：:The complete nucleotide sequence of a nerve growth factor precursor from Bothrops jararacussu snake (Bj-NGF) was determined by DNA sequencing of a clone from cDNA library prepared from the poly(A) + RNA of the venom gland of B. jararacussu. cDNA encoding Bj-NGF precursor contained 723 bp in length, which encoded a pre...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(02)01429-3
更新日期：2002-07-01 00:00:00
abstract：:A non-toxic, direct-acting fibrinolytic serine protease (Bafibrinase) demonstrating thrombolytic and anticoagulant properties was purified from Bacillus sp. strain AS-S20-I. Bafibrinase was monomeric, with a molecular mass of 32.3 kDa. The peptide mass fingerprinting of Bafibrinase revealed only 8.3% sequence coverage...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.02.027
更新日期：2012-06-01 00:00:00
abstract：:Anaerobiospirillum succiniciproducens phosphoenolpyruvate (PEP) carboxykinase catalyses the reversible metal-dependent formation of oxaloacetate (OAA) and ATP from PEP, ADP and CO(2). Mutations of PEP carboxykinase have been constructed where the residues His(225) and Asp(263), two residues of the enzyme's putative Mn...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2003.10.013
更新日期：2004-01-01 00:00:00
abstract：:Developing a logical and rational methodology for obtaining vaccines, especially against the main parasite causing human malaria (P. falciparum), consists of blocking receptor-ligand interactions. Conserved peptides derived from proteins involved in invasion and having high red blood cell binding ability have thus bee...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.07.006
更新日期：2006-02-01 00:00:00
abstract：:The present study was conducted on CD4(+) T cells, isolated from wild type (WT) and PPARalpha(null) mice, in order to assess the mechanism of action of docosahexaenoic acid (DHA), an n-3 fatty acid, in the modulation of two transcription factors, i.e., T-bet and GATA-3, implicated in T-cell differentiation towards, re...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.09.012
更新日期：2009-11-01 00:00:00
abstract：:Human prophospholipase A2 was purified from pancreatic juice. The protein has a molecular weight of 14500 and a free N-terminal residue identified as aspartic acid (or asparagine). The amino acid composition was determined. Partial immunological identity has been obtained between human and porcine prophospholipase A2....
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(81)80216-7
更新日期：1981-08-01 00:00:00
abstract：:The kinetic and regulatory properties of purified pigeon heart muscle AMP deaminase were investigated. In the presence of 100 mM potassium chloride, the enzyme exhibited a slightly sigmoidal type of kinetics. Addition of ATP to the incubation medium changed the reaction rate versus substrate concentration plot into a ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90243-4
更新日期：1987-02-01 00:00:00
abstract：:Kinetic properties of PGM1 and PGM2 phosphoglucomutase "primary" isoenzymes from human erythrocytes were studied. The two enzyme forms share a "ping-pong" kinetic mechanism and show similar Km for substrate (glucose 1-P) and cofactor (glucose 1,6-P2). Micromolar concentrations of fructose 1,6-P2 and glycerate 2,3-P2 i...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(84)90115-9
更新日期：1984-09-01 00:00:00
abstract：:Plant serine proteases have been widely used in food science and technology as well as in medicine. In this sense, several plant serine proteases have been proposed as potential anti-coagulants and anti-platelet agents. Previously, we have reported the purification and identification of a plant serine protease from So...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2016.03.015
更新日期：2016-06-01 00:00:00
abstract：:Leishmania parasites cause two million new cases of leishmaniasis each year with several hundreds of millions of people at risk. Due to the paucity and shortcomings of available drugs, we have undertaken the crystal structure determination of a key enzyme from Leishmania major in hopes of creating a platform for the r...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.11.015
更新日期：2011-03-01 00:00:00
abstract：:The potent cytotoxicity of reactive oxygen species (ROS) can cause various diseases, however, it may also serve as a powerful chemotherapeutic strategy capable of killing cancer cells. Oxalomalate (OMA, α-hydroxy-β-oxalosuccinic acid), a tricarboxylic acid intermediate, is a well-known competitive inhibitor of two cla...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.01.004
更新日期：2019-03-01 00:00:00
abstract：:DNA mismatch repair (MMR) is an important replication error avoidance mechanism that prevents mutation. The association of defective MMR with familial and sporadic gastrointestinal and endometrial cancer has been acknowledged for some years. More recently, it has become apparent that MMR defects are common in acute my...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2003.10.007
更新日期：2003-11-01 00:00:00
abstract：:The blue-green bile pigments of Actias selene (Attacidae) have been investigated at different stages of its development. Coproporphyrinogen-14-C, protoporphyrin-IX3-H, and pterobilin-14-C, injected to larvae are metabolised into phorcabilin I, the main neopterobilin in this animal. It is concluded that phorcabilin I i...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(75)80313-0
更新日期：1975-01-01 00:00:00
abstract：:Oxazole-containing macrocycles, which include the natural product telomestatin, represent a promising class of anticancer agents that target G-quadruplex DNA. Two synthetic hexaoxazole-containing macrocyclic compounds (HXDV and HXLV-AC) have been characterized with regard to their cytotoxic activities versus human can...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.03.011
更新日期：2008-08-01 00:00:00
abstract：:The Escherichia coli low molecular mass penicillin-binding proteins (PBP4, PBP5 and PBP6) are a group of penicillin-sensitive enzymes involved in the final stages of cell wall assembly. It has been suggested that these proteins may interact with the periplasmic face of the inner membrane via C-terminal amphiphilic alp...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)83502-x
更新日期：1997-04-01 00:00:00
abstract：:The G protein coupled receptors (GPCR) are an important class of proteins that act as signal transducers through the cytoplasmic membrane. Understanding the structure and activation mechanism of these proteins is crucial for understanding many different aspects of cellular signalling. The olfactory receptors correspon...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(98)80019-9
更新日期：1998-02-01 00:00:00
abstract：:EPR spectroscopy in conjunction with oxidation-reduction potentiometry has been used to determine the half-reduction potentials of the four hemes of cytochrome c3. As predicted, the four hemes of cytochrome c3 have different mid-point potentials. The Em values are: Heme I,--284 mV; Heme II,--310 mV; Heme III,--324 mV ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(78)80829-3
更新日期：1978-01-01 00:00:00
abstract：:Genome-editing technologies, in particular, CRISPR systems, are widely used for targeted regulation of gene expression and obtaining modified human and animal cell lines, plants, fungi, and animals with preassigned features. Despite being well described and easy to perform, the most common methods for construction and...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2019.09.003
更新日期：2019-12-01 00:00:00
abstract：:The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2010.05.009
更新日期：2010-11-01 00:00:00
abstract：:Recombinant plasmids containing head-to-tail copies of different coat-protein replacement genomes of wheat dwarf virus (WDV) were used to study the mechanism leading to the release of replicating unit-length molecules in suspension culture cells of Triticum monococcum. For plasmids bearing two complete genomes, the vi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90067-3
更新日期：1993-01-01 00:00:00
abstract：:After exhaustive pronase digestion, purification by gel filtration and affinity chromatography on concanavalin A, three glycopeptide fractions were obtained from rat hemopexin. Two fractions (I and II) were concanavalin A non-reactive and one (III) was concanavalin A reactive. On the basis of carbohydrate composition,...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(83)80083-2
更新日期：1983-03-01 00:00:00
abstract：:The hydrolysis of trioctanoin and of monoolein by rat intestinal homogenates, catalyzed by the previously described "trioctanoin hydrolase" and "monoglyceride lipase" [Ref. 6 and 11], was shown to be entirely due to the action of the intestinal glycerol-ester hydrolase [Ref. 1 and 2] and to contaminating pancreatic li...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80320-9
更新日期：1977-01-01 00:00:00
abstract：:The insulin receptor appears as a tetrameric glycoprotein consisting of two Mr 130,000 subunits (alpha), and two Mr 95,000 subunits (beta) in a disulfide-linked complex. Insulin bound to its specific cell surface receptors in its target cells leads to a complex array of molecular events resulting in insulin effects. I...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80109-7
更新日期：1985-10-01 00:00:00
abstract：:Acinetobacter baumannii encodes all enzymes required in the tricarboxylic acid (TCA) cycle and glyoxylate bypass except for isocitrate dehydrogenase kinase/phosphatase (IDHKP), which can phosphorylate isocitrate dehydrogenase (IDH) at a substrate-binding Ser site and control the carbon flux in enterobacteria, such as ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.12.001
更新日期：2020-12-05 00:00:00
abstract：:An unusual tri-domained (alpha-beta-beta) natural oyster metallothionein (MT) is known, and non-oxidative MT dimers occur in vivo in mollusk species and in mammals. To assess the respective role of the MT domains, two chimeric MTs were constructed: a tetra-domained oyster MT corresponding to the alpha-beta-alpha-beta ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.01.008
更新日期：2008-05-01 00:00:00
