cDNA sequence and molecular modeling of a nerve growth factor from Bothrops jararacussu venomous gland.

abstract：:Pseudomonas cepacia 122 can grow on several lignin model compounds including the arylglycerol-beta-aryl ethers guaiacylglycerol-beta-coniferyl ether and guaiacylglycerol-beta-guaiacyl ether. Non-phenolic lignin model compounds are not degraded by this bacterium. The enzyme system catalyzing guaiacylglycerol-beta-guaia...

journal_title：Biochimie

authors： Odier E,Rolando C

更新日期：1985-02-01 00:00:00

abstract：:We have modified elongation factor Tu (EF-Tu) from Escherichia coli via mutagenesis of its encoding tufA gene to study its function-structure relationships. The isolation of the N-terminal half molecule of EF-Tu (G domain) has facilitated the analysis of the basic EF-Tu activities, since the G domain binds the substra...

journal_title：Biochimie

authors： Anborgh PH,Cool RH,Gümüsel F,Harmark K,Jacquet E,Weijland A,Mistou MY,Parmeggiani A

更新日期：1991-07-01 00:00:00

abstract：:In most cases, anti-protein aptamers are selected by systematic evolution of ligands by exponential enrichment (SELEX) using purified recombinant protein targets. Cell surface proteins, however, are not easy targets for SELEX due to the difficulties associated with their purification. Here, we developed a novel SELEX ...

journal_title：Biochimie

authors： Ohuchi SP,Ohtsu T,Nakamura Y

更新日期：2006-07-01 00:00:00

abstract：:Chagasin-type inhibitors comprise natural inhibitors of papain-like cysteine proteases that are distributed among Protist, Bacteria and Archaea. Chagasin was identified in the pathogenic protozoa Trypanosoma cruzi as an approximately 11 kDa protein that is a tight-binding and highly thermostable inhibitor of papain, c...

journal_title：Biochimie

authors： Costa TF,Lima AP

更新日期：2016-03-01 00:00:00

abstract：:The polysaccharide from Aloe vahombe (lilaceae) was submitted to partial hydrolyses by sulfuric, oxalic and phosphoric acid. Some oligosaccharides were isolated and investigated by chemical and 13C NMR spectroscopic methods. Their structure was determined. The results prove unambiguously that in the analyzed material ...

journal_title：Biochimie

authors： Radjabi-Nassab F,Ramiliarison C,Monneret C,Vilkas E

更新日期：1984-07-01 00:00:00

abstract：:The adaptive response appears in E. coli after exposure to low levels of alkylating agents. This system is under the positive control of the ada gene. At least two enzymes are induced during the response: 3-methyladenine DNA glycosylase II and O6-methylguanine DNA methyltransferase. The latter is also the product of t...

journal_title：Biochimie

authors： Defais M

更新日期：1985-03-01 00:00:00

abstract：:The dissociation of bound polyribosomes from ewe lactating mammary gland, by EDTA or Puromycin-KC1 treatment releases Messenger Ribonucleoprotein particles (mRNP'S) which sediment in sucrose gradients as a single component having a sedimentation coefficient of about 22S. RNAs derived from mRNPs sediment between 9S and...

journal_title：Biochimie

authors： Assairi L,Gaye P,Houdebine LM

更新日期：1976-01-01 00:00:00

abstract：:Two-dimensional J-resolved and correlated 1H NMR spectra with complete water suppression have been obtained to further characterize a metabolic pattern for normal and pathological human plasma samples. 1H COSY spectra have been recorded on plasma from 12 patients with cancer in order to check for the possible presence...

journal_title：Biochimie

authors： Kriat M,Confort-Gouny S,Vion-Dury J,Viout P,Cozzone PJ

更新日期：1992-09-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae strain LB332 bearing a mutation in the ERG20 gene encoding farnesyl diphosphate synthase (FPPS) synthesizes significantly longer dolichols than the wild type strain FL100 (14-31 and 14-19 isoprene units, respectively). The measurement of the short chain prenyl alcohols excreted into ...

journal_title：Biochimie

authors： Plochocka D,Karst F,Swiezewska E,Szkopińska A

更新日期：2000-08-01 00:00:00

abstract：:The cellular concentration of cyclic nucleotides is largely dependent upon the activity of the enzymatic system responsible for their degradation: cyclic nucleotide phosphodiesterase. This enzymatic system thus plays a crucial role in the regulation of the multiple functions which are modulated by cyclic nucleotides i...

journal_title：Biochimie

authors： Nemoz G,Prigent AF

更新日期：1984-02-01 00:00:00

abstract：:Conformational flexibility and structural fluctuations play an important role in enzyme activity. A great variety of internal motions ranging over different time scales and of different amplitudes are involved in the catalytic cycle. These different types of motions and their functional consequences are considered in ...

journal_title：Biochimie

authors： Yon JM,Perahia D,Ghélis C

更新日期：1998-01-01 00:00:00

abstract：:LysK, the enzyme lysing cells of Staphylococcus aureus, can be considered as perspective antimicrobial agent. Knowledge of LysK properties and behavior would allow optimizing conditions of its storage as well as formulating strategy towards its stabilization. Reaction of LysK with substrate (suspension of autoclaved S...

journal_title：Biochimie

authors： Filatova LY,Becker SC,Donovan DM,Gladilin AK,Klyachko NL

更新日期：2010-05-01 00:00:00

abstract：:The chromatin of shrimp hepatopancreas has been extracted from isolated nuclei and characterized. Nuclei were prepared in the presence of Cu++ and phenyl methyl sulfonyl fluoride in order to inhibit the nuclease and protease activities throughout the different purification steps. The purified nuclei are heterogenous i...

journal_title：Biochimie

authors： Sellos D,Van Wormhoudt A

更新日期：1979-01-01 00:00:00

abstract：:A neoglycolipid of structure beta-D-Glcp-S-(CH2)3N(OH)(CH2)4-O-cholest-5-en-3 beta-yl has been prepared in fair overall yield by reduction of the nitrone obtained by condensation of beta-D-Glcp-S-(CH2)3NHOH and OCH-(CH2)3-O-cholest-5-en-3 beta-yl. This synthetic procedure is very flexible, allowing a large range of le...

journal_title：Biochimie

authors： Tronchet JM,Zsély M,Ricca A,Barbalat-Rey F,Geoffroy M

更新日期：1992-01-01 00:00:00

abstract：:An unusual tri-domained (alpha-beta-beta) natural oyster metallothionein (MT) is known, and non-oxidative MT dimers occur in vivo in mollusk species and in mammals. To assess the respective role of the MT domains, two chimeric MTs were constructed: a tetra-domained oyster MT corresponding to the alpha-beta-alpha-beta ...

journal_title：Biochimie

authors： Moreau JL,Baudrimont M,Carrier P,Peltier G,Bourdineaud JP

更新日期：2008-05-01 00:00:00

abstract：:Hydroxylamine and its derivatives of general formula H2NOR react with aldehydes and aldimines to produce oximes. If R corresponds to the side chain of a natural amino acid, such compounds can be thought of as analogs of the corresponding amino acids, lacking the alpha-carboxylate group. Oximes formed between such comp...

journal_title：Biochimie

authors： Delbaere LT,Kallen J,Markovic-Housley Z,Khomutov AR,Khomutov RM,Karpeisky MY,Jansonius JN

更新日期：1989-04-01 00:00:00

abstract：:Aptamers are short single-stranded nucleic acid molecules that are selected in vitro from a large random sequence library based on their high and specific affinity to a target molecule by a process known as SELEX. Cell-SELEX that employs whole living cells overexpressing the defined cell surface proteins (for selectio...

journal_title：Biochimie

authors： Takahashi M,Sakota E,Nakamura Y

更新日期：2016-12-01 00:00:00

abstract：:Our understanding of plant meiosis is rapidly increasing thanks to the model Arabidopsis thaliana. Here we present the results of a screening for meiotic mutants carried out with a library containing 30,719 T-DNA insertion lines. An average of one mutant per 1000 lines was recovered. Several phenotypic classes could b...

journal_title：Biochimie

authors： Mercier R,Grelon M,Vezon D,Horlow C,Pelletier G

更新日期：2001-11-01 00:00:00

abstract：:In relation to decreased metabolic sensitivity to catecholamines observed, in vitro, in brown fat of cold-acclimated rats, beta-adrenergic receptors were studied in isolated cells and in a crude membrane preparation from rat interscapular brown adipose tissue. [3H] dihydroalprenolol binding had the same characteristic...

journal_title：Biochimie

authors： Senault C,Le Comte V,Portet R

更新日期：1984-07-01 00:00:00

abstract：:P53 is a transcription factor also called the "cellular gatekeeper of genome" because it can induce cell cycle arrest in G1, apoptosis or affect DNA replication in response to DNA damage. Wild type p53 is localised in both the cytoplasm and nucleus of first trimester trophoblastic cells (CTB). Immunoblotting of CTB wi...

journal_title：Biochimie

authors： Cohen M,Wuillemin C,Bischof P

更新日期：2008-06-01 00:00:00

abstract：:In parallel with crystallographic studies of ribosomes from Thermus thermophilus, a long-term program on the crystallization and structural investigations of ribosomal proteins from the same microorganism has been started at the Institute of Protein Research (Pushchino, Russia). At present, more than half of the indiv...

journal_title：Biochimie

authors： Garber MB,Agalarov SC,Eliseikina IA,Fomenkova NP,Nikonov SV,Sedelnikova SE,Shikaeva OS,Vasiliev D,Zhdanov AS,Liljas A

更新日期：1992-04-01 00:00:00

abstract：:The quest for effective anticancer therapeutics continues to be extensively pursued. Over the past century, several drugs have been developed, however, a majority of these drugs have a poor therapeutic index and increased toxicity profile. Hence, there still exists ample opportunity to discover safe and effective anti...

journal_title：Biochimie

authors： Singh M,Malhotra L,Haque MA,Kumar M,Tikhomirov A,Litvinova V,Korolev AM,Ethayathulla AS,Das U,Shchekotikhin AE,Kaur P

更新日期：2021-01-06 00:00:00

abstract：:Lysophosphatidic acid (LPA), a naturally occurring bioactive phospholipid, mediates a multitude of (patho)physiological events including activation of mitogen-activated protein kinases (MAPKs). As LPA may induce cellular reponses in human osteosarcoma, the present study aimed at investigating expression of various LPA...

journal_title：Biochimie

authors： Windischhofer W,Huber E,Rossmann C,Semlitsch M,Kitz K,Rauh A,Devaney T,Leis HJ,Malle E

更新日期：2012-09-01 00:00:00

abstract：:We screened a cDNA library of the primitive eukaryote Dictyostelium discoideum constructed in the expression vector lambda gt11 with a specific antiserum directed against S-adenosyl-L-homocysteine hydrolase (AdoHcy hydrolase) and isolated cDNA clones coding for fusion proteins with beta-galactosidase. The identity of ...

journal_title：Biochimie

authors： Guitton MC,Part D,Veron M

更新日期：1988-06-01 00:00:00

abstract：:Present knowledge of the different enzymatic steps of the denitrification chains in various bacteria, particularly Paracoccus denitrificans and Pseudomonas aeruginosa has been briefly reviewed. The question whether nitric oxide (NO), nitrous oxide (N2O) and other nitrogen derivatives are obligatory intermediates has b...

journal_title：Biochimie

authors： Henry Y,Bessières P

更新日期：1984-04-01 00:00:00

abstract：:Wheat germ lysate was used as a model system for in vitro translation. We show that an increase of the exchange surface between the reaction mixture and the atmosphere enhanced the amount of incorporated cysteine, indicating that early arrest of protein synthesis previously observed in such a system was due to oxygen ...

journal_title：Biochimie

authors： Luciani N,Hess K,Belleville F,Nabet P

更新日期：1995-01-01 00:00:00

abstract：:It has been shown that two Proteus morganii strains produce an inducible cephalosporinase. No significative difference was shown between them: they present the same isoelectric poit: 8,3 and very similar kinetic constants. The parameter tau, proportional to the half life of antibiotic at low concentration, in presence...

journal_title：Biochimie

authors： Labia R,Guionie M,Fabre C

更新日期：1976-11-13 00:00:00

abstract：:DNA mismatch repair (MMR) is an important replication error avoidance mechanism that prevents mutation. The association of defective MMR with familial and sporadic gastrointestinal and endometrial cancer has been acknowledged for some years. More recently, it has become apparent that MMR defects are common in acute my...

journal_title：Biochimie

authors： Karran P,Offman J,Bignami M

更新日期：2003-11-01 00:00:00

abstract：:Administration of beta-adrenergic agonists to domestic species can lead to skeletal muscle hypertrophy, probably by reducing the rate of myofibrillar protein breakdown. Myofibrillar breakdown is associated with the calcium-dependent proteinase system (calpains I,II and calpastatin) whose activity also changes during b...

journal_title：Biochimie

authors： Bardsley RG,Allcock SM,Dawson JM,Dumelow NW,Higgins JA,Lasslett YV,Lockley AK,Parr T,Buttery PJ

更新日期：1992-03-01 00:00:00

abstract：:The kinetoplastid protozoan Leishmania encodes major surface glycoprotein GP63, a zinc metallo-peptidase (EC.3.4.24.36) expressed both in promastigote and amastigote life stages. In the present study, we explored for the first time the role of C-terminal domain (CTD) in proteinase activity by serial truncation of Leis...

journal_title：Biochimie

authors： Mazumder S,Ganguly A,Ali N

更新日期：2010-12-01 00:00:00