Abstract：
:We screened a cDNA library of the primitive eukaryote Dictyostelium discoideum constructed in the expression vector lambda gt11 with a specific antiserum directed against S-adenosyl-L-homocysteine hydrolase (AdoHcy hydrolase) and isolated cDNA clones coding for fusion proteins with beta-galactosidase. The identity of the largest of these clones was further assessed by analysis of hybrid-selected in vitro translation products. Using the cDNA as a probe, we showed that only one gene coding for AdoHcy hydrolase is present per genome and that a single transcript of 1.6 kb could be detected at various stages of differentiation. The nucleotide sequence of the cDNA was determined. It corresponds to the carboxyterminal half of the protein whose primary structure is highly homologous to the AdoHcy hydrolase from rat liver. The significance of this strong conservation of AdoHcy hydrolase in the course of evolution is discussed.
journal_name
Biochimiejournal_title
Biochimieauthors
Guitton MC,Part D,Veron Mdoi
10.1016/0300-9084(88)90115-0subject
Has Abstractpub_date
1988-06-01 00:00:00pages
835-40issue
6eissn
0300-9084issn
1638-6183pii
0300-9084(88)90115-0journal_volume
70pub_type
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