Abstract:
:The ascomycete Cladosporium herbarum is a prominent fungal inducer of Type I allergy. The only major allergen identified so far is Cla h 8, a NADP-dependent mannitol dehydrogenase (MtDH). MtDH, a cytoplasmic protein of 28.5kDa, belongs to the Short chain Dehydrogenases/Reductases (SDR), acting as a NADP-dependent oxidoreductase. In this study, we found that C. herbarum MtDH can exist as monomers, dimers and tetramers in solution and, correspondingly, forms tetramers and higher oligomers in two crystal structures. Additionally, we identified a unique adaptive binding site for the metal ions Na(+) and Zn(2+) that were distinguished by an anomalous dispersion experiment. A Translation-Libration-Screw analysis confirmed the stabilising effect of Zn(2+) for the tetrameric assembly. Moreover, the zinc containing structure explains the mode of MtDH multimerisation by metal bridging of the tetramers. The formation of oligomers and higher multimers of MtDH provides a missing link to its allergenic properties. Based on the well defined active site region and a comparative analysis with related structures, we can also clarify the atypical enzymatic properties of MtDH by two alternative binding modes of the substrate to the active site.
journal_name
Biochimiejournal_title
Biochimieauthors
Nüss D,Goettig P,Magler I,Denk U,Breitenbach M,Schneider PB,Brandstetter H,Simon-Nobbe Bdoi
10.1016/j.biochi.2010.04.012subject
Has Abstractpub_date
2010-08-01 00:00:00pages
985-93issue
8eissn
0300-9084issn
1638-6183pii
S0300-9084(10)00150-1journal_volume
92pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Tryptocidine C (TpcC, cyclo[D-Phe1-Pro2-Trp3-D-Trp4-Asn5-Gln6-Trp7-Val8-Orn9-Leu10]) is a broad-spectrum antimicrobial peptide in the tyrothricin complex produced by a soil bacterium, Brevibacillus parabrevis. Electrospray mass spectrometric studies reveal the oligomerisation of TpcC into dimers and higher oligomers, ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.12.006
更新日期:2020-12-18 00:00:00
abstract::The subunit composition of the myosin molecule which is built up from 3 pairs of identical polypeptide chains (2 heavy chains and 2 pairs of light chains), gives it the appearance of having symmetric structure. This homodimeric arrangement in the molecule is in fact asymmetric in its construction as a result of the na...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(81)80117-4
更新日期:1981-04-01 00:00:00
abstract::Insect prophenoloxidase (PPO) is an essential innate immunity protein to induce pathogen into melanization. In Bombyx mori, pro-phenoloxidase-activating enzyme (PPAE) can directly cleave and activate PPO. However, PPO in Manduca sexta cannot be cleaved into active phenoloxidase (PO) by serine proteases unless cofactor...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.03.007
更新日期:2014-07-01 00:00:00
abstract::Accumulating evidences suggest that Herba epimedii has the potential benefits against osteoporosis. However, previous studies were focused on the crude extract, total flavonoids (TF) and icariin (ICA), and the detailed molecular mechanisms of action and structure-activity relationship (SAR) remain unclear. Herein we a...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.06.033
更新日期:2012-12-01 00:00:00
abstract::The RecA protein of E coli promotes a strand exchange reaction in vitro which appears to be similar to homologous genetic recombination in vivo. A model for the mechanism of strand transfer reaction by RecA protein has been proposed by Howard-Flanders et al based on the assumption that the RecA monomer has two distinc...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90200-k
更新日期:1991-02-01 00:00:00
abstract::We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of v...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.07.013
更新日期:2006-02-01 00:00:00
abstract::The hydrolysis of trioctanoin and of monoolein by rat intestinal homogenates, catalyzed by the previously described "trioctanoin hydrolase" and "monoglyceride lipase" [Ref. 6 and 11], was shown to be entirely due to the action of the intestinal glycerol-ester hydrolase [Ref. 1 and 2] and to contaminating pancreatic li...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80320-9
更新日期:1977-01-01 00:00:00
abstract::The rat and rabbit lung isoform pattern before (fetal) and after (adult) lung maturation has been analyzed by isoelectric focusing. This pattern has been compared to that of brain tubulin at the same developmental stages. No changes in the pattern of fetal and adult lung tubulin were found. However an increase in brai...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80301-1
更新日期:1985-09-01 00:00:00
abstract::SR-PSOX can function as a scavenger receptor, a chemokine and an adhesion molecule, and it could be an interesting player in the formation of atherosclerotic lesions. Our previous studies demonstrated that basic amino acid residues in the chemokine domain of SR-PSOX are critical for its functions. In this study the co...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.09.016
更新日期:2013-02-01 00:00:00
abstract::The widespread nature of protein phosphorylation/dephosphorylation underscores its key role in cell signaling metabolism, growth and differentiation. Tyrosine phosphorylation of cytoplasmic proteins is a critical event in the regulation of intracellular signaling pathways activated by external stimuli. An adequate bal...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2010.01.002
更新日期:2010-04-01 00:00:00
abstract::An alkaline aminopeptidase was isolated from the culture medium of Penicillium roqueforti. The enzyme was purified by ammonium sulfate precipitation, filtration on Bio-Gel P-100, chromatography on D.E.A.E.-cellulose and hydroxylapatite, filtration on Bio-Gel P-150 and electrofusing. The purified preparation was homoge...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80246-0
更新日期:1977-01-01 00:00:00
abstract::Surfactants carrying either a hydrocarbon or a fluorocarbon alkyl chain have been synthesized. The polar head was either tris(hydroxymethyl)acrylamidomethane (THAM), telomerized THAM, or a glycosylated THAM moiety. The aqueous solubility of some of these molecules was increased by oxidizing to a sulfoxide the thioethe...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)80017-6
更新日期:1998-05-01 00:00:00
abstract::In the present study we have investigated the thermal stability of the globular transport protein human serum albumin (HSA), in the presence of two small chain polyethylene glycols (namely PEG 200 and PEG 400). Both near- and far-UV circular dichroism (CD) study reveal that addition of PEG moderately increases the α-h...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.05.009
更新日期:2014-09-01 00:00:00
abstract::Human prophospholipase A2 was purified from pancreatic juice. The protein has a molecular weight of 14500 and a free N-terminal residue identified as aspartic acid (or asparagine). The amino acid composition was determined. Partial immunological identity has been obtained between human and porcine prophospholipase A2....
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(81)80216-7
更新日期:1981-08-01 00:00:00
abstract::Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and e...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.07.025
更新日期:2013-11-01 00:00:00
abstract::In the present study we evaluated the effect of chronic methionine administration on oxidative stress and biochemical parameters in liver and serum of rats, respectively. We also performed histological analysis in liver. Results showed that hypermethioninemia increased chemiluminescence, carbonyl content and glutathio...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.04.018
更新日期:2009-08-01 00:00:00
abstract::DNA replication in Escherichia coli is controlled at the initiation stage, possibly by regulation of the essential activity of DnaA protein. The cellular membrane has long been hypothesized to be involved in chromosomal replication. Accumulating evidence, both in vitro and in vivo, that supports the importance of memb...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(00)01224-4
更新日期:2001-01-01 00:00:00
abstract::In parallel with crystallographic studies of ribosomes from Thermus thermophilus, a long-term program on the crystallization and structural investigations of ribosomal proteins from the same microorganism has been started at the Institute of Protein Research (Pushchino, Russia). At present, more than half of the indiv...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(92)90110-z
更新日期:1992-04-01 00:00:00
abstract::The oxidation of beta-D-glucose with glucose oxidase generally requires oxygen, which, under normal conditions is present at low concentrations in the reaction medium. Experiments show that glucose oxidase is no longer saturated by oxygen at enzyme concentrations greater than 0.4 mg.ml1. This is due to the decrease in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(75)80160-x
更新日期:1975-01-01 00:00:00
abstract::The G protein coupled receptors (GPCR) are an important class of proteins that act as signal transducers through the cytoplasmic membrane. Understanding the structure and activation mechanism of these proteins is crucial for understanding many different aspects of cellular signalling. The olfactory receptors correspon...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(98)80019-9
更新日期:1998-02-01 00:00:00
abstract::Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)88887-2
更新日期:1998-11-01 00:00:00
abstract::The polysaccharide mixture obtained by hot water extraction of Aloe vahombe leaves is composed of at least four different paritally acetylated glucomannans which differ in molecular weight, glucose to mannose ratios and acetyl contents. Furthermore, one fraction contains a small but significant amount of protein which...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(86)80187-0
更新日期:1986-09-01 00:00:00
abstract::Genome-editing technologies, in particular, CRISPR systems, are widely used for targeted regulation of gene expression and obtaining modified human and animal cell lines, plants, fungi, and animals with preassigned features. Despite being well described and easy to perform, the most common methods for construction and...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2019.09.003
更新日期:2019-12-01 00:00:00
abstract::Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.03.023
更新日期:2015-06-01 00:00:00
abstract::The short isoform of Rho guanine nucleotide exchange factor ARHGEF5 is known as TIM, which plays diverse roles in, for example, tumorigenesis, neuronal development and Src-induced podosome formation through the activation of its substrates, the Rho family of GTPases. The activation is auto-inhibited by a putative heli...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.01.011
更新日期:2015-04-01 00:00:00
abstract::Genetic elements coding for proteins that present amino acid identity with the conserved motifs of retroviral reverse transcriptases constitute the retroid family. With the exception of reverse transcriptases encoded by mitochondrial plasmids of Neurospora, all reverse transcriptases have an absolute requirement for a...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(96)88114-4
更新日期:1995-01-01 00:00:00
abstract::The targets of in vivo studies of the ribosomal assembly process are mainly the events of rRNA processing, whereas in vitro studies (total reconstitution) focus on principles of the assembly process such as assembly-initiation proteins, rate-limiting steps and a detailed sequence of assembly reactions (assembly map). ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(91)90054-5
更新日期:1991-06-01 00:00:00
abstract::The nuclear metabolism of poly(ADP-ribose) is mainly regulated by poly(ADP-ribose) polymerase-1 (PARP-1) and by poly(ADP-ribose) glycohydrolase (PARG). A PARP-like enzyme, V-PARP, and a PARG isoform are present in the extra-nuclear compartment of mammalian cells, even if poly(ADP-ribose) has never been detected therei...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(02)00017-2
更新日期:2002-12-01 00:00:00
abstract::Conformational changes induced by the binding of nickel to horseradish peroxidase C (HRPC) were studied by electronic absorption spectroscopy, fluorescence spectroscopy and circular dichroism spectroscopy. Incubation of HRPC with various concentrations of Ni(2+) for 5 minutes resulted in changes in the enzyme absorpti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.04.001
更新日期:2006-09-01 00:00:00
abstract::Hyper- (450 mOsm/l) and hypoosmotic exposure (150 mOsm/l) of Caco-2 cells, a human intestinal epithelial cell line, induced a twofold- and a fivefold increase in the production of IL-8, a constitutively expressed cytokine, respectively. This was observed both in the presence or in the absence of added proinflammatory ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.07.009
更新日期:2004-08-01 00:00:00