Selection of RNA aptamers against recombinant transforming growth factor-beta type III receptor displayed on cell surface.

abstract：:Insect prophenoloxidase (PPO) is an essential innate immunity protein to induce pathogen into melanization. In Bombyx mori, pro-phenoloxidase-activating enzyme (PPAE) can directly cleave and activate PPO. However, PPO in Manduca sexta cannot be cleaved into active phenoloxidase (PO) by serine proteases unless cofactor...

journal_title：Biochimie

authors： Lu A,Li X,Hillyer JF,Beerntsen BT,Söderhäll K,Ling E

更新日期：2014-07-01 00:00:00

abstract：:Calcium-independent receptor of alpha-latrotoxin (CIRL-1) is an adhesion G protein-coupled receptor implicated in the regulation of exocytosis. CIRL-1 biosynthesis involves constitutive proteolytic processing that takes place in the endoplasmic reticulum, requires the receptor's GPS domain, and yields heterologous two...

journal_title：Biochimie

authors： Deyev IE,Petrenko AG

更新日期：2010-04-01 00:00:00

abstract：:Changes in the cytosolic Ca(2+) concentration ([Ca(2+)](c)) translate a variety of extracellular signals into widely diverse intracellular effects, ranging from secretion to movement, proliferation and also cell death. As regards the last one, it has long been known that large [Ca(2+)](c) increases lead cells to death...

journal_title：Biochimie

authors： Pinton P,Ferrari D,Rapizzi E,Di Virgilio F,Pozzan T,Rizzuto R

更新日期：2002-02-01 00:00:00

abstract：:An 18.2 kDa protein from the liver fluke, Fasciola hepatica has been identified and characterised. The protein shows strongest sequence similarity to egg antigen proteins from Schistosoma mansoni, Schistosoma japonicum and Clonorchis sinensis. The protein is predicted to adopt a calmodulin-like fold; it thus represent...

journal_title：Biochimie

authors： Russell SL,McFerran NV,Moore CM,Tsang Y,Glass P,Hoey EM,Trudgett A,Timson DJ

更新日期：2012-11-01 00:00:00

abstract：:The phosphotriesterase PTE, identified in the soil bacterium Pseudomonas diminuta, is thought to have evolved in the last several decades to degrade the pesticide paraoxon with proficiency approaching the limit of substrate diffusion (k(cat)/K(M) of 4 x 10(7)M(-1)s(-1)). It belongs to the amidohydrolase superfamily, b...

journal_title：Biochimie

authors： Porzio E,Merone L,Mandrich L,Rossi M,Manco G

更新日期：2007-05-01 00:00:00

abstract：:A nuclear poly(ADP-ribose) polymerase (PARP) is activated by gamma-irradiation and consequently synthesizes poly(ADP-ribose) by binding to DNA strand-breaks. This property suggests that PARP is a DNA strand-break-signal generator. Meanwhile, the cell-cycle arrest occurs in G1 and G2 phases following gamma-irradiation....

journal_title：Biochimie

authors： Masutani M,Nozaki T,Wakabayashi K,Sugimura T

更新日期：1995-01-01 00:00:00

abstract：:Several inositol isomers and in particular myo-inositol (MI) and D-chiro-inositol (DCI), were shown to possess insulin-mimetic properties and to be efficient in lowering post-prandial blood glucose. In addition, abnormalities in inositol metabolism are associated with insulin resistance and with long term microvascula...

journal_title：Biochimie

authors： Croze ML,Soulage CO

更新日期：2013-10-01 00:00:00

abstract：:The adaptive response appears in E. coli after exposure to low levels of alkylating agents. This system is under the positive control of the ada gene. At least two enzymes are induced during the response: 3-methyladenine DNA glycosylase II and O6-methylguanine DNA methyltransferase. The latter is also the product of t...

journal_title：Biochimie

authors： Defais M

更新日期：1985-03-01 00:00:00

abstract：:The action of indomethacin on the lactogenic activity of prolactin has been evaluated usind the technique of rabbit mammary gland organ culture. Indomethacin is totally unable to inhibit prolactin action as estimated by lactose synthetase activity and casein synthesis. These data suggest, as opposed to previous works...

journal_title：Biochimie

authors： Houdebine LM,Lacroix MC

更新日期：1980-01-01 00:00:00

abstract：:Knowing the structure of a protein is essential to characterize its function and mechanism at the molecular level. Despite major advances in solving structures experimentally, most membrane protein native conformations remain unknown. This lack of available structures, along with the physical constraints imposed by th...

journal_title：Biochimie

authors： Postic G,Ghouzam Y,Gelly JC

更新日期：2015-08-01 00:00:00

abstract：:Experimental Lyme arthritis provides a mouse model for exploring the development of pathology following infection of C3H mice with Borrelia burgdorferi. Infected mice develop a reliable inflammatory arthritis of the ankle joint with severity that typically peaks around two to three weeks post-infection and then underg...

journal_title：Biochimie

authors： Brown CR,Dennis EA

更新日期：2017-10-01 00:00:00

abstract：:We screened a cDNA library of the primitive eukaryote Dictyostelium discoideum constructed in the expression vector lambda gt11 with a specific antiserum directed against S-adenosyl-L-homocysteine hydrolase (AdoHcy hydrolase) and isolated cDNA clones coding for fusion proteins with beta-galactosidase. The identity of ...

journal_title：Biochimie

authors： Guitton MC,Part D,Veron M

更新日期：1988-06-01 00:00:00

abstract：:Zika and Dengue viruses have attracted substantial attention from researchers in light of recent outbreaks of Dengue fever and increases in cases of congenital microcephaly in areas with Zika incidence. This review summarizes the current state of knowledge about Zika and Dengue proteases. These enzymes have several in...

journal_title：Biochimie

authors： Majerová T,Novotný P,Krýsová E,Konvalinka J

更新日期：2019-11-01 00:00:00

abstract：:Intracellular cytoplasmic peptidoglycan (PG) intermediate levels were determined in Staphylococcus aureus during log-phase growth in enriched media. Levels of UDP-linked intermediates were quantitatively determined using ion pairing LC-MS/MS in negative mode, and amine intermediates were quantitatively determined ster...

journal_title：Biochimie

authors： Vemula H,Ayon NJ,Gutheil WG

更新日期：2016-02-01 00:00:00

abstract：:The mitochondria have arisen as a consequence of endosymbiosis of an ancestral α-proteobacterium with a methane-producing archae. The main function of the canonical aerobic mitochondria include ATP generation via oxidative phosphorylation, heme and phospholipid synthesis, calcium homeostasis, programmed cell death, an...

journal_title：Biochimie

authors： Makiuchi T,Nozaki T

更新日期：2014-05-01 00:00:00

abstract：:Two soluble forms of brain glutamate dehydrogenase isoproteins were inactivated by pyridoxal 5'-phosphate. Restoration of catalytic activity can be accomplished by dialysis and addition of an excess of cysteine or lysine. Spectral evidence is presented to indicate that the inactivation proceeds through Schiff base for...

journal_title：Biochimie

authors： Cho SW,Lee JE

更新日期：1996-01-01 00:00:00

abstract：:The aim of the present work was to establish the thermodynamic and functional differences in the protein-protein interactions between the components of the P450-dependent mitochondrial (mit) and microsomal (mic) monooxygenase systems using 12 different isoforms of cytochromes P450 and two redox partners, NADPH-depende...

journal_title：Biochimie

authors： Yablokov EO,Sushko TA,Ershov PV,Florinskaya AV,Gnedenko OV,Shkel TV,Grabovec IP,Strushkevich NV,Kaluzhskiy LA,Usanov SA,Gilep AA,Ivanov AS

更新日期：2019-07-01 00:00:00

abstract：:Colorectal cancer (CRC) is the third most diagnosed cancer in the western world, affecting 1 out of approximately 22 people in their lifetime. Several epidemiological studies suggest a positive association between high plasma cholesterol levels and colorectal cancer. However, the molecular mechanisms by which choleste...

journal_title：Biochimie

authors： Warns J,Marwarha G,Freking N,Ghribi O

更新日期：2018-10-01 00:00:00

abstract：:Aptamers are short single-stranded nucleic acid molecules that are selected in vitro from a large random sequence library based on their high and specific affinity to a target molecule by a process known as SELEX. Cell-SELEX that employs whole living cells overexpressing the defined cell surface proteins (for selectio...

journal_title：Biochimie

authors： Takahashi M,Sakota E,Nakamura Y

更新日期：2016-12-01 00:00:00

abstract：:Glycogen synthase I was purified from bovine polymorphonuclear leucocytes (PMNs) by a procedure involving concanavalin A-Sepharose affinity chromatography. The purified glycogen-bound glycogen synthase I had a specific activity of 9.83 U/mg protein and the glycogen free enzyme 21 U/mg protein. Molecular ratio of the n...

journal_title：Biochimie

authors： Rasmussen LH,Pedersen KM,Juhl H

更新日期：1985-06-01 00:00:00

abstract：:Triiodothyronine (T3), injected daily into rats, induces heart hypertrophy. In a recent work we have shown that a single T3 injection stimulates RNA synthesis and modifies the translational products of myocardial mRNAs in reticulocyte lysates, quantitatively and qualitatively. In this work we show that T3 induces smal...

journal_title：Biochimie

authors： Raymondjean M,Leibovitch SA,Leibovitch MP,Harel J,Kruh J

更新日期：1984-09-01 00:00:00

abstract：:The intervention of pyruvate in glucose metabolism was investigated during hypoxic stress in tumour cell cultures having respiratory capacities under normoxic conditions. Results obtained with nuclear magnetic resonance (NMR) spectroscopy showed that, under normoxic conditions, rat glioma C6 and human hepatoma Hep G2 ...

journal_title：Biochimie

authors： Perrin A,Roudier E,Duborjal H,Bachelet C,Riva-Lavieille C,Leverve X,Massarelli R

更新日期：2002-10-01 00:00:00

abstract：:Cytochromes P450 constitute a superfamily of hemoproteins which have evolved from a common ancestor. Recent advances in molecular biology have offered a powerful approach to the research on the multiplicity of P450. Now, every mammalian P450 species can be characterized by heterologous expression of its cDNA. In addit...

journal_title：Biochimie

authors： Yabusaki Y

更新日期：1995-01-01 00:00:00

abstract：:Histidine-proline-rich glycoprotein (HPRG), or histidine-rich glycoprotein (HRG), is a serum protein that is synthesized in the liver and is actively internalised by different cells, including skeletal muscle. The multidomain arrangement of HPRG comprises two modules at the N-terminus that are homologous to cystatin b...

journal_title：Biochimie

authors： Ronca F,Raggi A

更新日期：2015-11-01 00:00:00

abstract：:We have previously identified a protein with Mr approximately 40,000 (p40) that binds with high specificity and affinity to the 5'-untranslated leaders of mitochondrial mRNAs in yeast. Here we show that this protein is abundant, comprising about 0.4% of total mitochondrial protein. p40 is present in a cytoplasmic (rho...

journal_title：Biochimie

authors： Dekker PJ,Papadopoulou B,Grivell LA

更新日期：1991-12-01 00:00:00

abstract：:The structure of ATP synthase subunit 4 was determined by using the oligonucleotide probe procedure. This subunit is the fourth polypeptide of the complex when classifying subunits in order of decreasing molecular mass. Its relative molecular mass is 25 kDa. The ATP4 gene was isolated and sequenced. The nucleotide seq...

journal_title：Biochimie

authors： Velours J,Arselin G,Paul MF,Galante M,Durrens P,Aigle M,Guerin B

更新日期：1989-08-01 00:00:00

abstract：:Botulinum neurotoxins (BoNT, serotypes A-G) and tetanus neurotoxin (TeNT) are bacterial proteins that comprise a light chain (M(r) approximately 50) disulfide linked to a heavy chain (M(r) approximately 100). By inhibiting neurotransmitter release at distinct synapses, these toxins cause two severe neuroparalytic dise...

journal_title：Biochimie

authors： Humeau Y,Doussau F,Grant NJ,Poulain B

更新日期：2000-05-01 00:00:00

abstract：:The polysaccharide mixture obtained by hot water extraction of Aloe vahombe leaves is composed of at least four different paritally acetylated glucomannans which differ in molecular weight, glucose to mannose ratios and acetyl contents. Furthermore, one fraction contains a small but significant amount of protein which...

journal_title：Biochimie

authors： Vilkas E,Radjabi-Nassab F

更新日期：1986-09-01 00:00:00

abstract：:Excessive tissue iron levels are associated with the increase of oxidative/nitrative stress which contributes to tissue damage that may elevate the risk of diabetes. Therefore, we investigated the effects of iron on diabetes-associated liver injury and whether iron-related tyrosine nitration participated in this proce...

journal_title：Biochimie

authors： Li X,Li H,Lu N,Feng Y,Huang Y,Gao Z

更新日期：2012-12-01 00:00:00

abstract：:By use of procollagen proline hydroxylas which catalyses the substitution of the hydrogen in the trans position on carbon 4 with an hydroxyl on L-proline residues of procollagen, we have shown that the L-[3H-4] proline product of C.E.N. Saclay contained 29 per cent of the trans and 71 per cent of the cis form. It was ...

journal_title：Biochimie

authors： Farjanel J,Pichat L,Audinot M,Perier C,Frey J

更新日期：1976-01-01 00:00:00