Abstract：
:Insect prophenoloxidase (PPO) is an essential innate immunity protein to induce pathogen into melanization. In Bombyx mori, pro-phenoloxidase-activating enzyme (PPAE) can directly cleave and activate PPO. However, PPO in Manduca sexta cannot be cleaved into active phenoloxidase (PO) by serine proteases unless cofactors are involved, which indicates that PPO activation is complicated. Here we use recombinant Drosophila melanogaster prophenoloxidase 1 (rPPO1) to study the mechanism of PPO activation induced by a typical serine protease, α-chymotrypsin. Small amounts of α-chymotrypsin cleave rPPO1 at the N- and C-terminus to produce a large fragment rPPO1(N1/C1) that needs further cleavage by α-chymotrypsin to produce a smaller fragment rPO1(60-kD) with PO activity. rPO1(60-kD) oxidizes dopamine without being affected by high temperature, or by having salt and Ethylene diamine tetraacetic acid (EDTA) in the solution. After incubation with dopamine, rPO1(60-kD) cannot be detected using reducing SDS-PAGE due to formation of a large complex. Trypsin, another typical serine protease, cleaves rPPO1 at the N- and C-terminus to produce a small fragment rPPO1(N'/C') without PO activity. Several rPPO1 mutants were created through over-expressing active fragments that have direct PO activity. They are easily cleaved by low amounts of α-chymotrypsin without increasing PO activity. Therefore, rPPO1 can be sequentially cleaved in at least three places by α-chymotrypsin to produce activated rPO1(60-kD).
journal_name
Biochimiejournal_title
Biochimieauthors
Lu A,Li X,Hillyer JF,Beerntsen BT,Söderhäll K,Ling Edoi
10.1016/j.biochi.2014.03.007subject
Has Abstractpub_date
2014-07-01 00:00:00pages
154-65eissn
0300-9084issn
1638-6183pii
S0300-9084(14)00087-Xjournal_volume
102pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Over the last decades, mesenchymal stem cells (MSCs) have been extensively studied with regard to their potential applications in regenerative medicine. In rheumatic diseases, MSC-based therapy is the subject of great expectations for patients who are refractory to proposed treatments such as rheumatoid arthritis (RA)...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2013.04.017
更新日期：2013-12-01 00:00:00
abstract：:The wheat germ translation initiation factor 2 (WGeIF-2) was isolated in a homogeneous state by an efficient procedure and characterized. Its molecular mass, as determined by a gel-filtration method is approximately 150,000 Da. According to SDS-PAGE WGeIF-2 consists of four subunits with M(r) 37,000 (alpha), 40,000 (b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(92)90085-s
更新日期：1992-05-01 00:00:00
abstract：:Nitrogen fixation in A. vinelandii and R. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the ATP/ADP-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. Although we have been successful in preparing in...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(78)80820-7
更新日期：1978-01-01 00:00:00
abstract：:Trypsin and pronase treatment of purified human neutral bronchial mucins released small fragments from the C-terminal end of these molecules and resulted in slight increases in their sedimentation coefficient presumably reflecting conformational changes. The antigenic determinant of neutral bronchial mucins which appe...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80444-0
更新日期：1976-01-01 00:00:00
abstract：:It has been shown that two Proteus morganii strains produce an inducible cephalosporinase. No significative difference was shown between them: they present the same isoelectric poit: 8,3 and very similar kinetic constants. The parameter tau, proportional to the half life of antibiotic at low concentration, in presence...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80086-7
更新日期：1976-11-13 00:00:00
abstract：:Replication protein A (RPA) is a heterotrimeric single-stranded DNA binding protein whose role in DNA replication, recombination and repair has been mainly elucidated through in vitro biochemical studies utilizing the mammalian complex. However, the identification of homologs of all three subunits in Saccharomyces cer...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(00)00183-8
更新日期：2000-01-01 00:00:00
abstract：:The molecular basis for the essential role of cholesterol in mammalian (and other cholesterol-requiring) cells has long been the object of intense interest. Cholesterol has been found to modulate the function of membrane proteins critical to cellular function. Current literature supports two mechanisms for this modula...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90093-g
更新日期：1991-10-01 00:00:00
abstract：:Hydroxylamine and its derivatives of general formula H2NOR react with aldehydes and aldimines to produce oximes. If R corresponds to the side chain of a natural amino acid, such compounds can be thought of as analogs of the corresponding amino acids, lacking the alpha-carboxylate group. Oximes formed between such comp...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(89)90175-2
更新日期：1989-04-01 00:00:00
abstract：:A series of halomethylated derivatives of dihydrocoumarins has been found to inhibit irreversibly proteases and esterases. alpha-Chymotrypsin, subtilish, elastase are rapidly inactivated in the presence of these compounds, while trypsin, kallicrein, papain are inhibited more slowly. Esterases like acetylcholinesterase...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80140-5
更新日期：1977-01-01 00:00:00
abstract：:The amino acid sequence of rat thymus histone obtained in highly purified form by preparative electrophoresis, was determined. This sequence is identical to the sequence of calf thymus histone H2B. The in vitro phosphorylation of the rat histone with a cyclic AMP-dependent protein kinase isolated from rat pancreas led...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(79)80313-2
更新日期：1979-01-01 00:00:00
abstract：:Glutathione, being a major intracellular redox regulator has been shown to be implicated in regulation of airway reactivity and inflammation. However, no study so far has investigated the effect of glutathione depletion/repletion during sensitization and challenge phases separately, which could provide an important in...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.04.001
更新日期：2014-08-01 00:00:00
abstract：:Protein-induced DNA bridging and looping is a common mechanism for various and essential processes in bacterial chromosomes. This mechanism is preserved despite the very different bacterial conditions and their expected influence on the thermodynamic and kinetic characteristics of the bridge formation and stability. O...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2017.08.002
更新日期：2017-11-01 00:00:00
abstract：:Three trends are seen in the organization of ribosomal DNA genes during evolution: 1) gradual separation and separability of the regulation of transcription of 5S and larger subunit rRNAs; 2) retention of a transcription unit containing both large and small rRNAs; and 3) clustering of genes for both 5S and 18S-28S rDN...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90042-y
更新日期：1991-06-01 00:00:00
abstract：:Using a rapid FPLC procedure for the separation of calpain I, calpain II and the inhibitor calpastatin from pork meat, results of proteolytic and inhibitory activity were obtained. Duplicate measurements showed variation coefficients less than 0.13. Compared to others we found lower calpain I activity 1 h post-mortem....
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90041-p
更新日期：1993-01-01 00:00:00
abstract：:TcrX/Y is one of the twelve two component system (TCS) present in Mycobacterium tuberculosis. We have investigated the TcrX/Y interaction by in silico studies, pull down assay, radioactive phosphotransfer, surface plasmon resonance as well as crosstalk analysis of TcrY with TcrA - a non-cognate response regulator. Seq...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.11.009
更新日期：2010-03-01 00:00:00
abstract：:In this work, were studied the conditions of erucic acid (cis-docosenoic, n-9) shortening by using Rat liver mitochondrial preparations which were incubated in vitro with [14-14C] erucic acid (22:1), with inhibitors of the respiratory chain (rotenone, cyanide) or not, with activators of either the shortening reaction ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(82)80352-0
更新日期：1982-10-01 00:00:00
abstract：:Botulinum neurotoxins (BoNT, serotypes A-G) and tetanus neurotoxin (TeNT) are bacterial proteins that comprise a light chain (M(r) approximately 50) disulfide linked to a heavy chain (M(r) approximately 100). By inhibiting neurotransmitter release at distinct synapses, these toxins cause two severe neuroparalytic dise...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(00)00216-9
更新日期：2000-05-01 00:00:00
abstract：:pH-Dependence of hydrolytic activity of trypsin has been studied in cationic reverse micellar system of cetyltrimethylammonium bromide (CTAB) in (50% v/v) chloroform/isooctane using a positively charged substrate N(alpha)-benzoyl-L-arginine ethyl ester (BAEE). The pH of the medium was varied from 4.0 to 8.5 with addit...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(99)80007-8
更新日期：1998-12-01 00:00:00
abstract：:A set of sequences can be defined by their common subsequences, and the length of these is a measure of the overall resemblance of the set. Each subsequence corresponds to a succession of symbols embedded in every sequence, following the same order but not necessarily contiguous. Determining the longest common subsequ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80271-6
更新日期：1985-05-01 00:00:00
abstract：:In the present study we evaluated the effect of chronic methionine administration on oxidative stress and biochemical parameters in liver and serum of rats, respectively. We also performed histological analysis in liver. Results showed that hypermethioninemia increased chemiluminescence, carbonyl content and glutathio...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.04.018
更新日期：2009-08-01 00:00:00
abstract：:Canine S100A12 (cS100A12) is a calcium-binding protein of the S100 superfamily of EF-hand proteins, and its expression is restricted to neutrophils and monocytes. Interaction of S100A12 with the receptor for advanced glycation end products (RAGE) has been suggested to play a central role in inflammation. Moreover, S10...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.08.007
更新日期：2010-12-01 00:00:00
abstract：:Pleurocidin (GWGSFFKKAAHVGKHVGKAALTHYL-NH(2)), found in skin mucous secretions of the winter flounder Pleuronectes americanus, is known to possess a high potency and broad-spectrum antimicrobial peptide without cytotoxicity. In this study, to investigate the impact of pleurocidin on apoptotic progress, we observed mor...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.07.011
更新日期：2011-10-01 00:00:00
abstract：:Translational control is a common mechanism used to regulate gene expression and occur in bacteria to mammals. Typically in translational control, an RNA-binding protein binds to a unique sequence in the mRNA to regulate protein synthesis by the ribosomes. Alternatively, a protein may bind to or modify a translation f...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.02.005
更新日期：2015-07-01 00:00:00
abstract：:Lysosomal cysteine proteinases were fractionated from partially purified rat muscle lysosomes. By gel filtration on Sephadex G75, cathepsin D was separated from two thiol-requiring proteolytic fractions of Mr 25 000 and 55 000, respectively. By chromatofocusing, the first fraction (Mr = 25 000) was resolved into three...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(84)90114-7
更新日期：1984-09-01 00:00:00
abstract：:Skin aging is characterized by different features including wrinkling, atrophy of the dermis and loss of elasticity associated with damage to the extracellular matrix protein elastin. The aim of this study was to investigate the aging process of skin elastin at the molecular level by evaluating the influence of intrin...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2016.08.010
更新日期：2016-09-01 00:00:00
abstract：:Plant vacuolar invertases, which belong to family 32 of glycoside hydrolases (GH32), are key enzymes in sugar metabolism. They hydrolyse sucrose into glucose and fructose. The cDNA encoding a vacuolar invertase from Solanum lycopersicum (TIV-1) was cloned and heterologously expressed in Pichia pastoris. The functional...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.12.013
更新日期：2014-06-01 00:00:00
abstract：:Many natural phyto-products as perezone (Per) exhibit anti-cancer activities. Using experimental and computational studies, it was described that Poly ADP-ribose polymerase 1(PARP-1) inhibition and the induction of oxidative stress state explain the pro-apoptotic activity of Per. The aim of this study was to evaluate ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.03.003
更新日期：2020-01-01 00:00:00
abstract：:The quantitative formalism called Metabolic Control Theory makes it possible to be precise in discussions of metabolic control. To illustrate this, I will mention 2 experimental systems where free energy is converted from one form to another, i.e., bacteriorhodopsin liposomes and mitochondrial oxidative phosphorylatio...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(89)90071-0
更新日期：1989-08-01 00:00:00
abstract：:The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80316-7
更新日期：1977-01-01 00:00:00
abstract：:For efficient DNA hydrolysis, Type III restriction endonuclease EcoP15I interacts with two inversely oriented recognition sites in an ATP-dependent process. EcoP15I consists of two methylation (Mod) subunits and a single restriction (Res) subunit yielding a multifunctional enzyme complex able to methylate or to hydrol...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.11.014
更新日期：2013-04-01 00:00:00
