Abstract：
:Skin aging is characterized by different features including wrinkling, atrophy of the dermis and loss of elasticity associated with damage to the extracellular matrix protein elastin. The aim of this study was to investigate the aging process of skin elastin at the molecular level by evaluating the influence of intrinsic (chronological aging) and extrinsic factors (sun exposure) on the morphology and susceptibility of elastin towards enzymatic degradation. Elastin was isolated from biopsies derived from sun-protected or sun-exposed skin of differently aged individuals. The morphology of the elastin fibers was characterized by scanning electron microscopy. Mass spectrometric analysis and label-free quantification allowed identifying differences in the cleavage patterns of the elastin samples after enzymatic digestion. Principal component analysis and hierarchical cluster analysis were used to visualize differences between the samples and to determine the contribution of extrinsic and intrinsic aging to the proteolytic susceptibility of elastin. Moreover, the release of potentially bioactive peptides was studied. Skin aging is associated with the decomposition of elastin fibers, which is more pronounced in sun-exposed tissue. Marker peptides were identified, which showed an age-related increase or decrease in their abundances and provide insights into the progression of the aging process of elastin fibers. Strong age-related cleavage occurs in hydrophobic tropoelastin domains 18, 20, 24 and 26. Photoaging makes the N-terminal and central parts of the tropoelastin molecules more susceptible towards enzymatic cleavage and, hence, accelerates the age-related degradation of elastin.
journal_name
Biochimiejournal_title
Biochimieauthors
Mora Huertas AC,Schmelzer CE,Hoehenwarter W,Heyroth F,Heinz Adoi
10.1016/j.biochi.2016.08.010subject
Has Abstractpub_date
2016-09-01 00:00:00pages
163-73eissn
0300-9084issn
1638-6183pii
S0300-9084(16)30158-4journal_volume
128-129pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:NDPK-A, NDPK-B and NDPK-D are three enzymes which belong to the NDPK group I isoforms and are not only involved in metabolism process but also in transcriptional regulation, DNA cleavage, histidine protein kinase activity and metastasis development. Those enzymes were reported to bind to membranes either in mitochondr...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.06.025
更新日期：2014-10-01 00:00:00
abstract：:Mitochondria are essential organelles and consequently proper expression and maintenance of the mitochondrial genome are indispensable for proper cell function. The mitochondrial Suv3 (SUPV3L1) helicase is known to have a central role in mitochondrial RNA metabolism and to be essential for maintenance of mitochondrial...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.11.003
更新日期：2015-01-01 00:00:00
abstract：:We have proposed an allosteric ATP inhibition mechanism of Pfk-2 determining the structure of different forms of the enzyme together with a kinetic enzyme analysis. Here we complement the mechanism by using hybrid oligomers of the homodimeric enzyme to get insights about the allosteric communication pathways between t...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2016.08.013
更新日期：2016-09-01 00:00:00
abstract：:This review article outlines some potentials and limits of the recent application of high resolution Nuclear Magnetic Resonance technique--coupled to the Fourier transformation methods--to the study of biological membranes. Molecular arrangement and dynamical structure characters can be assessed at the level of indivi...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(75)80333-6
更新日期：1975-01-01 00:00:00
abstract：:Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.03.023
更新日期：2015-06-01 00:00:00
abstract：:Viral expression was analyzed in ten cell clones of a Friend erythroleukemia cell line (HFL/b cell line [3]), which had lost its capacity to produce infectious particles. All the ten subclones were non producers but expressed spleen focus forming virus (SFFV) polypeptides in the form of p48-p50gag and gp50-gp52env. On...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(83)80277-6
更新日期：1983-04-01 00:00:00
abstract：:Chronic hypobaric hypoxia induced muscle atrophy results in decreased physical performance at high altitude. Curcumin has been shown to have muscle sparing effects under cachectic conditions. However, the protective effects of curcumin under chronic hypobaric hypoxia have not been studied till now. Therefore, the pres...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.10.012
更新日期：2019-01-01 00:00:00
abstract：:Triple-negative breast cancer (TNBC) is a heterogeneous group of breast cancer with one common feature: distinctly metastatic nature with higher rate of relapse and shorter survival compared with other subtypes of breast cancer. The epithelial to mesenchymal transition (EMT) is highly associated with cancer metastasis...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.03.004
更新日期：2019-07-01 00:00:00
abstract：:During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90077-e
更新日期：1991-01-01 00:00:00
abstract：:Datura stramonium contains a compound that impairs learning retention in mice. It has been purified to homogeneity and its structure has been established as that of a gamma-L-glutamyl-L-aspartate. The biological activity of this pseudodipeptide has been found to be identical with that of the corresponding synthetic on...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90183-6
更新日期：1988-09-01 00:00:00
abstract：:In most cancer cells, the ATP necessary for survival and proliferation is derived from glycolysis rather than from oxidative phosphorylations (OXPHOS) even when oxygen supply would be adequate to sustain them. This phenomenon, named "aerobic glycolysis" by Warburg many years ago, can now be explained by a mechanism up...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2007.03.010
更新日期：2007-09-01 00:00:00
abstract：:As part of a project aimed at obtaining selective inhibitors and drug-like compounds targeting tRNA synthetases from trypanosomatids, we have elucidated the crystal structure of human cytosolic histidyl-tRNA synthetase (Hs-cHisRS) in complex with histidine in order to be able to compare human and parasite enzymes. The...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.08.005
更新日期：2014-11-01 00:00:00
abstract：:Cytochromes P450 constitute a superfamily of hemoproteins which have evolved from a common ancestor. Recent advances in molecular biology have offered a powerful approach to the research on the multiplicity of P450. Now, every mammalian P450 species can be characterized by heterologous expression of its cDNA. In addit...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(96)88175-2
更新日期：1995-01-01 00:00:00
abstract：:Concern about the malicious applications of botulinum neurotoxin has highlighted the need for a new generation of safe and highly potent antitoxins. In this study, we developed and evaluated the preclinical pharmacology and safety of a new F(ab')₂ antitoxin against botulinum neurotoxin serotype A (BoNT/A). As an alter...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.06.010
更新日期：2010-10-01 00:00:00
abstract：:Extracellular Hsp70 (eHsp70) can activate immune cells via Toll-like receptors (TLR) 2 and 4, and induce cytokine synthesis. The aim of this study was to explore inflammation-associated effects of eHsp70 alone and in combination with cigarette smoke extract (CSE) in primary bronchial epithelial cells. We assessed IL-6...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.09.010
更新日期：2019-01-01 00:00:00
abstract：:Regeneration is an adaptive phenomenon with wide biological implications spread heterogeneously in almost all the organism including human beings. The ability of regeneration varies from species to species for its complexity. Epimorphic regeneration of zebrafish caudal fin tissue is the most widely studied regeneratio...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.05.014
更新日期：2020-08-01 00:00:00
abstract：:Beta-turmerin from turmeric (Curcuma longa) waste grits obtained after extraction of curcumin was purified by successive gel permeation chromatography. Homogeneity of beta-turmerin was confirmed by its movement as single band both in SDS-PAGE and as well as in native (basic) PAGE. The apparent molecular mass is approx...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.06.006
更新日期：2009-09-01 00:00:00
abstract：:Sphingosine is a natural sphingolipid found in membranes of all eukaryotic cells. In addition to its functions in cell signaling, sphingosine has broad-spectrum antimicrobial properties. Sphingosine's role as an antimicrobial is important in tissues such as the skin and respiratory epithelium. Reduction in the normal ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.03.014
更新日期：2017-10-01 00:00:00
abstract：:The mouse interferon alpha 7 gene, the signal sequence of which has been removed by oligonucleotide-directed mutagenesis, was introduced into a Bacillus subtilis secretion vector containing the promoter and the signal sequence of the B. subtilis levansucrase gene. Different B. subtilis strains were transformed with th...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(89)90091-6
更新日期：1989-06-01 00:00:00
abstract：:The rat and rabbit lung isoform pattern before (fetal) and after (adult) lung maturation has been analyzed by isoelectric focusing. This pattern has been compared to that of brain tubulin at the same developmental stages. No changes in the pattern of fetal and adult lung tubulin were found. However an increase in brai...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80301-1
更新日期：1985-09-01 00:00:00
abstract：:Surfactants carrying either a hydrocarbon or a fluorocarbon alkyl chain have been synthesized. The polar head was either tris(hydroxymethyl)acrylamidomethane (THAM), telomerized THAM, or a glycosylated THAM moiety. The aqueous solubility of some of these molecules was increased by oxidizing to a sulfoxide the thioethe...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(00)80017-6
更新日期：1998-05-01 00:00:00
abstract：:Influenza virus-associated RNA polymerase is composed of one molecule each of three viral P proteins and carries the complete activity of capped RNA-primed vRNA-directed transcription. The RNA polymerase holoenzyme also carries capped RNA endonuclease to generate capped oligonucleotide primers for transcription and 3'...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(97)86735-1
更新日期：1996-01-01 00:00:00
abstract：:The specific binding of the amyloid protein precursor (APP) to glycosaminoglycans (GAG) suggests that APP is a cell adhesion molecule (CAM) and/or substrate adhesion molecule (SAM). In order to characterize this activity of APP in the brain at the molecular level, we have purified and characterized the major APP speci...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(94)90163-5
更新日期：1994-01-01 00:00:00
abstract：:Nephropathy is one of the most frequent complications of chronic diabetes. The main reason for nephropathy despite being hyperglycemia, but it progresses even after good glycemic control has been achieved in diabetic patients. The effects of prior exposure to high blood glucose conditions depend upon the severity and ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2020.01.007
更新日期：2020-03-01 00:00:00
abstract：:Strains of Escherichia, Salmonella, Shigella and Yersinia actively enter eukaryotic cells. Several techniques were used to compare and contrast the invasion mechanisms of Salmonella cholerae-suis, Yersinia enterocolitica and Shigella flexneri. Three animal cell lines (CHO, HEp-2 and MDCK) were examined for susceptibil...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90271-4
更新日期：1988-08-01 00:00:00
abstract：:Tetramolecular G-quadruplexes result from the association of four guanine-rich strands. Modification of the backbone strand or the guanine bases of the oligonucleotide may improve stability or introduce new functionalities. In this regard, the 8 position of a guanosine is particularly suitable for introduction of modi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.10.011
更新日期：2011-03-01 00:00:00
abstract：:pH-Dependence of hydrolytic activity of trypsin has been studied in cationic reverse micellar system of cetyltrimethylammonium bromide (CTAB) in (50% v/v) chloroform/isooctane using a positively charged substrate N(alpha)-benzoyl-L-arginine ethyl ester (BAEE). The pH of the medium was varied from 4.0 to 8.5 with addit...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(99)80007-8
更新日期：1998-12-01 00:00:00
abstract：:In recent years an increasing number of inherited decreases in man has been identified in which there is an impairment of one or more peroxisomal functions. Sofar 15 different peroxisomal disorders have been identified which can be subdivided into three distinct groups depending upon whether there is a generalized (gr...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(93)90087-9
更新日期：1993-01-01 00:00:00
abstract：:EPR spectroscopy in conjunction with oxidation-reduction potentiometry has been used to determine the half-reduction potentials of the four hemes of cytochrome c3. As predicted, the four hemes of cytochrome c3 have different mid-point potentials. The Em values are: Heme I,--284 mV; Heme II,--310 mV; Heme III,--324 mV ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(78)80829-3
更新日期：1978-01-01 00:00:00
abstract：:The insulin receptor appears as a tetrameric glycoprotein consisting of two Mr 130,000 subunits (alpha), and two Mr 95,000 subunits (beta) in a disulfide-linked complex. Insulin bound to its specific cell surface receptors in its target cells leads to a complex array of molecular events resulting in insulin effects. I...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80109-7
更新日期：1985-10-01 00:00:00
