Pro-inflammatory effects of extracellular Hsp70 and cigarette smoke in primary airway epithelial cells from COPD patients.

Abstract:

:Extracellular Hsp70 (eHsp70) can activate immune cells via Toll-like receptors (TLR) 2 and 4, and induce cytokine synthesis. The aim of this study was to explore inflammation-associated effects of eHsp70 alone and in combination with cigarette smoke extract (CSE) in primary bronchial epithelial cells. We assessed IL-6 and IL-8 concentrations, TLR2, TLR4 and Hsp70 mRNA expressions, and mitogen-activated protein kinases (MAPKs) activation induced by recombinant human (rh) Hsp70, CSE or their combinations in normal human bronchial epithelial cells (NHBE) obtained commercially, and primary bronchial epithelial cells isolated from non-COPD lung donors (PBEC) or COPD patients (PBEC COPD). Baseline levels of IL-6 and IL-8 were significantly higher in PBEC COPD than in non-COPD PBECs. Upon rhHsp70 stimulation, IL-6 and IL-8 were significantly increased, with the strongest response in COPD-derived PBECs. CSE alone elevated cytokine secretion in all examined cells. rhHsp70 and CSE had antagonistic interactions on IL-8 release in PBECs from COPD patients, while the addition of rhHsp70 further increased CSE-induced IL-6 secretion in NHBE cells. rhHsp70 and CSE alone decreased TLR2 and TLR4 mRNA expression in COPD-derived PBECs. In non-COPD PBECs, combined treatments decreased only TLR2 mRNA expression. Hsp70 mRNA expression, as indicator of intracellular Hsp70, which may have anti-inflammatory effects, was reduced in COPD-derived cells upon exposure to CSE and rhHsp70 alone, but not with their combinations. Contrary to this, in PBECs from lung donors only combined treatments supressed Hsp70 gene expression. CSE activated JNK and p38 MAPKs, while rhHsp70 increased activation of c-Jun kinase in NHBE cells. Collectively, both eHsp70 and CSE induce pro-inflammatory responses in PBECs from non-COPD as well as COPD donors, but in combination antagonistic effects were observed in COPD-derived cells. These effects may be related to the regulation of TLR2/4 and might lead to modulation of inflammation with possible deleterious consequences for COPD patients.

journal_name

Biochimie

journal_title

Biochimie

authors

Hulina-Tomašković A,Heijink IH,Jonker MR,Somborac-Bačura A,Grdić Rajković M,Rumora L

doi

10.1016/j.biochi.2018.09.010

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

47-58

eissn

0300-9084

issn

1638-6183

pii

S0300-9084(18)30270-0

journal_volume

156

pub_type

杂志文章
  • L-lactate oxidase from Aerococcus viridans crystallized as an octamer. Preliminary X-ray studies.

    abstract::Crystals of flavoenzyme L-lactate oxidase from Aerococcus viridans (LOX) have been obtained that diffract to 3.0 A resolution (P2(1)2(1)2(1), a = 118.4 A, b = 138.4 A, c = 194.6 A). Crystallographic studies suggest that the enzyme may exist as an octameric form with non-crystallographic two- and four-fold axes in the ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(98)80072-2

    authors: Morimoto Y,Yorita K,Aki K,Misaki H,Massey V

    更新日期:1998-04-01 00:00:00

  • Characteristics of the interaction of a treponemal hemolysin with rabbit erythrocytes.

    abstract::The mechanism of action on rabbit red cells of Treponema hyodysenteriae hemolysin was studied using volume analysis and release of hemoglobin. While fixation of the hemolysin on the erythrocytes is temperature independent, it appears that hemolysis is temperature dependent. The kinetics of hemolysis proceed according ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(80)80134-9

    authors: Saheb SA,Lafleur L

    更新日期:1980-01-01 00:00:00

  • The global regulator LaeA controls penicillin biosynthesis, pigmentation and sporulation, but not roquefortine C synthesis in Penicillium chrysogenum.

    abstract::The biosynthesis of the beta-lactam antibiotic penicillin is an excellent model for the study of secondary metabolites produced by filamentous fungi due to the good background knowledge on the biochemistry and molecular genetics of the beta-lactam producing microorganisms. The three genes (pcbAB, pcbC, penDE) encoding...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2008.09.004

    authors: Kosalková K,García-Estrada C,Ullán RV,Godio RP,Feltrer R,Teijeira F,Mauriz E,Martín JF

    更新日期:2009-02-01 00:00:00

  • Unusual interaction of human apurinic/apyrimidinic endonuclease 1 (APE1) with abasic sites via the Schiff-base-dependent mechanism.

    abstract::Clustered apurinic/apyrimidinic (AP) sites are more cytotoxic than isolated AP lesions because double strand breaks (DSB) can be formed during repair of closely positioned bistranded AP sites. Formation of DSB due to simultaneous cleavage of bistranded AP sites may be regulated by proteins specifically interacting wit...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2018.04.027

    authors: Ilina ES,Khodyreva SN,Lavrik OI

    更新日期:2018-07-01 00:00:00

  • Anti-angiogenic activities of CRBGP from buccal glands of lampreys (Lampetra japonica).

    abstract::Cysteine-rich secretory proteins (CRISPs), characterized by 16 conserved cysteines, are distributed in a wide range of organisms, such as secernenteas, amphibians, reptiles and mammals. In the previous studies, a novel CRISP family member (cysteine-rich buccal gland protein, CRBGP) was separated from the buccal gland ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2015.11.020

    authors: Jiang Q,Liu Y,Duan D,Gou M,Wang H,Wang J,Li Q,Xiao R

    更新日期:2016-04-01 00:00:00

  • Comparison of the invasion strategies used by Salmonella cholerae-suis, Shigella flexneri and Yersinia enterocolitica to enter cultured animal cells: endosome acidification is not required for bacterial invasion or intracellular replication.

    abstract::Strains of Escherichia, Salmonella, Shigella and Yersinia actively enter eukaryotic cells. Several techniques were used to compare and contrast the invasion mechanisms of Salmonella cholerae-suis, Yersinia enterocolitica and Shigella flexneri. Three animal cell lines (CHO, HEp-2 and MDCK) were examined for susceptibil...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(88)90271-4

    authors: Finlay BB,Falkow S

    更新日期:1988-08-01 00:00:00

  • In vitro translation of human pheochromocytoma messenger RNAs: characterization of tyrosine-hydroxylase and dopamine-beta-hydroxylase.

    abstract::mRNAs extracted from human pheochromocytoma were translated in vitro in a lysate of a rabbit reticulocytes. Two enzymes of the biosynthetic pathway of the catecholamines, tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH), were characterized as translation products after immunoprecipitation by specific anti...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(85)80198-x

    authors: Benlot C,Antreassian J,Henry JP,Legrand JC,Gros F,Thibault J

    更新日期:1985-06-01 00:00:00

  • Thermodynamic investigation of kissing-loop interactions.

    abstract::Kissing loop interactions (KLIs) are a common motif that is critical in retroviral dimerization, viroid replication, mRNA, and riboswitches. In addition, KLIs are currently used in a variety of biotechnology applications, such as in aptamer sensors, RNA scaffolds and to stabilize vaccines for therapeutics. Here we des...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2018.11.012

    authors: Carr CE,Marky LA

    更新日期:2019-02-01 00:00:00

  • Pseudouridylation of 23S rRNA helix 69 promotes peptide release by release factor RF2 but not by release factor RF1.

    abstract::Pseudouridine [Ψ] is a frequent base modification in the ribosomal RNA [rRNA] and may be involved in the modulation of the conformational flexibility of rRNA helix-loop structures during protein synthesis. Helix 69 of 23S rRNA contains pseudouridines at the positions 1911, 1915 and 1917 which are formed by the helix 6...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2010.12.018

    authors: Kipper K,Sild S,Hetényi C,Remme J,Liiv A

    更新日期:2011-05-01 00:00:00

  • Computer generation and statistical analysis of a data bank of protein sequences translated from GenBank.

    abstract::We describe PGtrans, a new and freely available protein sequence databank (2625 sequences, 554198 amino-acids). This data bank is routinely produced by automatic computer translation of the nucleotide sequence library GenBank. The information needed for the translation process (transcriptional orientation, location of...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(85)80261-3

    authors: Claverie JM,Sauvaget I,Bougueleret L

    更新日期:1985-05-01 00:00:00

  • Hydration of transfer RNA molecules: a crystallographic study.

    abstract::Four crystal structures of transfer RNA molecules were refined at 3 A resolution with the inclusion of the solvent molecules found in the difference maps: yeast tRNA-phe in the orthorhombic form, yeast tRNA-phe in the monoclinic form and yeast tRNA-asp in the A and B forms. Over 100 solvent molecules were located in e...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/0300-9084(88)90056-9

    authors: Westhof E,Dumas P,Moras D

    更新日期:1988-02-01 00:00:00

  • The increased accumulation of structurally modified versican and decorin is related with the progression of laryngeal cancer.

    abstract::Versican and decorin, two proteoglycans (PGs) with contradictory roles in the pathophysiology of cancer, comprise important stromal components in many tumor types and play a crucial role in the progression of cancer. In this study, we provide direct evidence for a significant and stage-related accumulation of versican...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2006.03.011

    authors: Skandalis SS,Theocharis AD,Papageorgakopoulou N,Vynios DH,Theocharis DA

    更新日期:2006-09-01 00:00:00

  • Purification and properties of glycogen synthase I from bovine polymorphonuclear leucocytes.

    abstract::Glycogen synthase I was purified from bovine polymorphonuclear leucocytes (PMNs) by a procedure involving concanavalin A-Sepharose affinity chromatography. The purified glycogen-bound glycogen synthase I had a specific activity of 9.83 U/mg protein and the glycogen free enzyme 21 U/mg protein. Molecular ratio of the n...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(85)80201-7

    authors: Rasmussen LH,Pedersen KM,Juhl H

    更新日期:1985-06-01 00:00:00

  • DspA from Strongylocentrotus purpuratus: The first biochemically characterized haloalkane dehalogenase of non-microbial origin.

    abstract::Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and e...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2013.07.025

    authors: Fortova A,Sebestova E,Stepankova V,Koudelakova T,Palkova L,Damborsky J,Chaloupkova R

    更新日期:2013-11-01 00:00:00

  • Evidence for a phosphoenolpyruvate dependent sugar-phosphotransferase system in the mollicute Acholeplasma florum.

    abstract::In order to confirm the presence of a phosphoenolpyruvate (PEP)-dependent sugar-phosphotransferase system (PTS) in the mollicute Acholeplasma florum we studied the ability of cell free extracts of this organism to phosphorylate glucose and/or fructose in the presence of PEP. We also cloned and sequenced a DNA fragment...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(93)90098-d

    authors: Navas-Castillo J,Laigret F,Hocquellet A,Chang CJ,Bove JM

    更新日期:1993-01-01 00:00:00

  • Structural and functional consequences of subunit interactions in glyceraldehyde 3-phosphate dehydrogenase.

    abstract::A variety of species of GPDH undergo acylation at two of the four active cystein sites per mole of tetrameric enzyme. This reaction requires tightly bound NAD+, a situation restricted to two of the four NAD sites per tetramer. S leads to N acyl transfer from cysteins to lysine in the diacyl enzyme yields an inactive e...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(81)80177-0

    authors: Malhotra OP,Bernhard SA,Seydoux F

    更新日期:1981-02-01 00:00:00

  • In vitro effect of caffeine on some aspects of nitrogen metabolism in isolated rat hepatocytes.

    abstract::Long-term exposure to high doses of caffeine affects several aspects of nitrogen metabolism, such as purine, pyrimidine and urea synthesis. However, little is known about the mechanisms of these changes and if they occur at shorter term. We have studied in isolated hepatocytes: 1) the in vitro effect of high doses of ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(88)90014-4

    authors: Jordá A,Saéz GT,Portolés M,Pallardó FV,Jimenez-Nacher I,Gascoó E

    更新日期:1988-10-01 00:00:00

  • Cloning of a cDNA for the S-adenosyl-L-homocysteine hydrolase from Dictyostelium discoideum.

    abstract::We screened a cDNA library of the primitive eukaryote Dictyostelium discoideum constructed in the expression vector lambda gt11 with a specific antiserum directed against S-adenosyl-L-homocysteine hydrolase (AdoHcy hydrolase) and isolated cDNA clones coding for fusion proteins with beta-galactosidase. The identity of ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(88)90115-0

    authors: Guitton MC,Part D,Veron M

    更新日期:1988-06-01 00:00:00

  • Two-dimensional 1H NMR spectroscopy of normal and pathological human plasma: complete water suppression and further assignment of resonances.

    abstract::Two-dimensional J-resolved and correlated 1H NMR spectra with complete water suppression have been obtained to further characterize a metabolic pattern for normal and pathological human plasma samples. 1H COSY spectra have been recorded on plasma from 12 patients with cancer in order to check for the possible presence...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(92)90075-p

    authors: Kriat M,Confort-Gouny S,Vion-Dury J,Viout P,Cozzone PJ

    更新日期:1992-09-01 00:00:00

  • Neuronal sphingolipidoses: Membrane lipids and sphingolipid activator proteins regulate lysosomal sphingolipid catabolism.

    abstract::Glycosphingolipids and sphingolipids of cellular plasma membranes (PMs) reach luminal intra-lysosomal vesicles (LVs) for degradation mainly by pathways of endocytosis. After a sorting and maturation process (e.g. degradation of sphingomyelin (SM) and secretion of cholesterol), sphingolipids of the LVs are digested by ...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2016.05.004

    authors: Sandhoff K

    更新日期:2016-11-01 00:00:00

  • A new phosphotriesterase from Sulfolobus acidocaldarius and its comparison with the homologue from Sulfolobus solfataricus.

    abstract::The phosphotriesterase PTE, identified in the soil bacterium Pseudomonas diminuta, is thought to have evolved in the last several decades to degrade the pesticide paraoxon with proficiency approaching the limit of substrate diffusion (k(cat)/K(M) of 4 x 10(7)M(-1)s(-1)). It belongs to the amidohydrolase superfamily, b...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2007.01.007

    authors: Porzio E,Merone L,Mandrich L,Rossi M,Manco G

    更新日期:2007-05-01 00:00:00

  • LysK, the enzyme lysing Staphylococcus aureus cells: specific kinetic features and approaches towards stabilization.

    abstract::LysK, the enzyme lysing cells of Staphylococcus aureus, can be considered as perspective antimicrobial agent. Knowledge of LysK properties and behavior would allow optimizing conditions of its storage as well as formulating strategy towards its stabilization. Reaction of LysK with substrate (suspension of autoclaved S...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2010.01.026

    authors: Filatova LY,Becker SC,Donovan DM,Gladilin AK,Klyachko NL

    更新日期:2010-05-01 00:00:00

  • Molecular parameters involved in bee-plant relationships: a biological and chemical approach.

    abstract::Honeybee-plant relationships are based on a conditioning process in which olfactory (plant aroma) and gustatory cues (mainly nectars) are closely linked, leading to a selective foraging behaviour. Among crops dependent upon entomophilous cross-pollination, the sunflower has recently undergone extensive expansion due t...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(87)90186-6

    authors: Pham-Delegue MH,Etievant P,Masson C

    更新日期:1987-06-01 00:00:00

  • Biochemical changes induced by strontium ranelate in differentiating adipocytes.

    abstract::Low bone formation in osteoporosis is associated with a shift from osteoblastic to adipogenic differentiation of mesenchymal stem cells (MSC) inducing a concomitant lipotoxic milieu within the bone marrow. Strontium ranelate (SrRN), a treatment for osteoporosis, has both anti-resorptive and anabolic effects on bone. T...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2012.11.008

    authors: Vidal C,Gunaratnam K,Tong J,Duque G

    更新日期:2013-04-01 00:00:00

  • Lactic acid bacteria: model systems for in vivo studies of sugar transport and metabolism in gram-positive organisms.

    abstract::Lactic acid bacteria provide a model system for the in vivo study of mechanisms pertaining to the regulation of sugar transport and metabolism by microorganisms. Recent studies with resting and growing cells of the homofermentative Streptococci and Lactobacilli have yielded evidence for hitherto unsuspected regulatory...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/0300-9084(88)90205-2

    authors: Thompson J

    更新日期:1988-03-01 00:00:00

  • Low enantioselectivities of human deoxycytidine kinase and human deoxyguanosine kinase with respect to 2'-deoxyadenosine, 2'-deoxyguanosine and their analogs.

    abstract::The antiviral activity of L-nucleoside analogs depends in part on the enantioselectivity of nucleoside kinases which catalyse their monophosphorylation. The substrate properties of human recombinant deoxycytidine kinase (dCK) and human recombinant deoxyguanosine kinase (dGK) with respect to L-adenosine and L-guanosine...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(99)00331-4

    authors: Gaubert G,Gosselin G,Boudou V,Imbach JL,Eriksson S,Maury G

    更新日期:1999-11-01 00:00:00

  • Variations in some molecular events during the early phases of the Reuber H 35 cell cycle. II.-Chromatin protein phosphorylation and protein kinases.

    abstract::Reuber H 35 hepatoma cells were synchronized by transfer in a serum free medium. Growth was re-initiated by addition of serum. Under these conditions DNA synthesis exhibited a maximum after 24 hours. Chromatin non-histone proteins prepared from cells at various phases of the cell cycle were incubated with [gamma-32P] ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(79)80280-1

    authors: Defer N,Tichonicky L,Kruh J,Zoutewelle G,Van Wijk R

    更新日期:1979-01-01 00:00:00

  • Measurement of translational accuracy in vivo: missense reporting using inactive enzyme mutants.

    abstract::The measurement and potential technological significance of in vivo missense errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the alpha subu...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/0300-9084(91)90192-4

    authors: Cornut B,Willson RC

    更新日期:1991-12-01 00:00:00

  • The crystal structure of ferritin from Chlorobium tepidum reveals a new conformation of the 4-fold channel for this protein family.

    abstract::Ferritins are ubiquitous iron-storage proteins found in all kingdoms of life. They share a common architecture made of 24 subunits of five α-helices. The recombinant Chlorobium tepidum ferritin (rCtFtn) is a structurally interesting protein since sequence alignments with other ferritins show that this protein has a si...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2014.07.019

    authors: Arenas-Salinas M,Townsend PD,Brito C,Marquez V,Marabolli V,Gonzalez-Nilo F,Matias C,Watt RK,López-Castro JD,Domínguez-Vera J,Pohl E,Yévenes A

    更新日期:2014-11-01 00:00:00

  • Intracellular pH control in Dictyostelium discoideum: a 31P-NMR analysis.

    abstract::Phosphorus metabolites and intracellular pH have been examined in the slime mold Dictyostelium discoideum by non-destructive 31P-NMR measurements. In a spectrum from a suspension of aerobic amoebae, the major peaks are inorganic phosphate, nucleotide di- and triphosphates. In the corresponding perchloric acid extract,...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(86)80077-3

    authors: Satre M,Klein G,Martin JB

    更新日期:1986-12-01 00:00:00