Variations in some molecular events during the early phases of the Reuber H 35 cell cycle. II.-Chromatin protein phosphorylation and protein kinases.

Abstract:

:Reuber H 35 hepatoma cells were synchronized by transfer in a serum free medium. Growth was re-initiated by addition of serum. Under these conditions DNA synthesis exhibited a maximum after 24 hours. Chromatin non-histone proteins prepared from cells at various phases of the cell cycle were incubated with [gamma-32P] ATP and the radioactive pattern of protein bound 32P was analysed by electrophoresis on polyacrylamide gels. No radioactive peak was observed in G0. Several peaks appeared 3 hours after the addition of serum. The radioactivity progressively increased until the cells reached the S phase. When most of the cells were in the S phase the radioactivity strongly decreased. Chromatin protein kinase activities were found to increase in late G1 and continued to increase in the S phase. The increase was 65% when phosvitin was the substrate, 100% with casein and histone H1. It is suggested that chromatin phosphorylated proteins could be involved in the mechanism which initiates DNA synthesis in G1 phase cells.

journal_name

Biochimie

journal_title

Biochimie

authors

Defer N,Tichonicky L,Kruh J,Zoutewelle G,Van Wijk R

doi

10.1016/s0300-9084(79)80280-1

subject

Has Abstract

pub_date

1979-01-01 00:00:00

pages

855-9

issue

7

eissn

0300-9084

issn

1638-6183

pii

S0300-9084(79)80280-1

journal_volume

61

pub_type

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