Abstract:
:Reuber H 35 hepatoma cells were synchronized by transfer in a serum free medium. Growth was re-initiated by addition of serum. Under these conditions DNA synthesis exhibited a maximum after 24 hours. Chromatin non-histone proteins prepared from cells at various phases of the cell cycle were incubated with [gamma-32P] ATP and the radioactive pattern of protein bound 32P was analysed by electrophoresis on polyacrylamide gels. No radioactive peak was observed in G0. Several peaks appeared 3 hours after the addition of serum. The radioactivity progressively increased until the cells reached the S phase. When most of the cells were in the S phase the radioactivity strongly decreased. Chromatin protein kinase activities were found to increase in late G1 and continued to increase in the S phase. The increase was 65% when phosvitin was the substrate, 100% with casein and histone H1. It is suggested that chromatin phosphorylated proteins could be involved in the mechanism which initiates DNA synthesis in G1 phase cells.
journal_name
Biochimiejournal_title
Biochimieauthors
Defer N,Tichonicky L,Kruh J,Zoutewelle G,Van Wijk Rdoi
10.1016/s0300-9084(79)80280-1subject
Has Abstractpub_date
1979-01-01 00:00:00pages
855-9issue
7eissn
0300-9084issn
1638-6183pii
S0300-9084(79)80280-1journal_volume
61pub_type
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