Abstract:
:The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene expression. After failing to obtain 3T3-F442A stable transfectants, we studied different techniques designed to enhance the efficiency of adenoviral transduction in fat cells. First, we compared the effects of two agents known to significantly enhance adenoviral transgene transduction, namely the cationic lipid lipofectamine and the cationic polymer polylysine. We show here that lipofectamine-assisted adenoviral transduction was more efficient in 3T3-F442A than in 3T3-L1 preadipocytes at all tested multiplicity of infection. Lipofectamine, and more efficiently polylysine, yielded high and sustained levels of adenoviral transgene expression in 3T3-F442A preadipocytes. Adenoviral transgene expression was maintained throughout the differentiation process. Furthermore, the two agents also efficiently enhanced adenoviral transduction in mature 3T3-F442A adipocytes. Interestingly, neither protocol affected the differentiation process, morphological features or protein expression of mature adipocytes. These approaches could be of interest to study fat cell differentiation and the functions of mature adipocytes.
journal_name
Biochimiejournal_title
Biochimieauthors
Béréziat V,Moritz S,Klonjkowski B,Decaudain A,Auclair M,Eloit M,Capeau J,Vigouroux Cdoi
10.1016/j.biochi.2005.05.010keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
951-8issue
11eissn
0300-9084issn
1638-6183pii
S0300-9084(05)00139-2journal_volume
87pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::LysK, the enzyme lysing cells of Staphylococcus aureus, can be considered as perspective antimicrobial agent. Knowledge of LysK properties and behavior would allow optimizing conditions of its storage as well as formulating strategy towards its stabilization. Reaction of LysK with substrate (suspension of autoclaved S...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.01.026
更新日期:2010-05-01 00:00:00
abstract::Pterocarpans, a family of isoflavonoids found in the diverse Fabaceae, display potent cytotoxic activity over a panel of tumor cell lines, and among those tested, 2,3,9- trimethoxypterocarpan displays the most potent activity. This study evaluates the effects of 2,3,9-trimethoxypterocarpan and its related derivatives ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.06.005
更新日期:2014-09-01 00:00:00
abstract::Elongation factor 1 alpha (EF-1 alpha) was purified to homogeneity from full-grown oocytes of Xenopus laevis. This protein is encoded by a gene previously shown to be expressed in male and female germ cells, and repressed in somatic cells. The purified protein was identified with EF-1 alpha on criteria of molecular ma...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90011-o
更新日期:1991-09-01 00:00:00
abstract::Betaine aldehyde dehydrogenase from the human opportunistic pathogen Pseudomonas aeruginosa (PaBADH) catalyzes the irreversible, NAD(P)(+)-dependent oxidation of betaine aldehyde, producing glycine betaine, an osmoprotectant. PaBADH participates in the catabolism of choline and likely in the defense against the osmoti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.09.022
更新日期:2011-02-01 00:00:00
abstract::Glutathione, being a major intracellular redox regulator has been shown to be implicated in regulation of airway reactivity and inflammation. However, no study so far has investigated the effect of glutathione depletion/repletion during sensitization and challenge phases separately, which could provide an important in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.04.001
更新日期:2014-08-01 00:00:00
abstract::Antibodies to Guo-AF were elicited in rabbits by immunization with bovine serum albumin -Guo-AF conjugate. The antibodies were purified by affinity chromatography. The affinity of the antibodies towards several ligands was studied by radioimmunoassays. The antibodies recognize Gua-AAF and Guo-AF (K(Guo-AF)/K(Guo-AAF) ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80066-6
更新日期:1980-01-01 00:00:00
abstract::Some oxysterols resulting either from enzymatic oxidation or autoxidation of cholesterol are associated with age-related diseases including neurodegenerative diseases. Among these oxysterols, 7β-hydroxycholesterol (7β-OHC) is often found at increased levels in patients. It is therefore important to identify molecules ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.06.027
更新日期:2018-10-01 00:00:00
abstract::We screened a cDNA library of the primitive eukaryote Dictyostelium discoideum constructed in the expression vector lambda gt11 with a specific antiserum directed against S-adenosyl-L-homocysteine hydrolase (AdoHcy hydrolase) and isolated cDNA clones coding for fusion proteins with beta-galactosidase. The identity of ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90115-0
更新日期:1988-06-01 00:00:00
abstract::Three Plasmodium falciparum serine repeat antigen (SERA) protein peptides were studied by NMR and structure calculations being done in 70:30 water:trifluoroethanol solution. Peptide 22834 was shown to be immunogenic and protective against malaria in Aotus monkeys, whilst native peptide 6737 and its analogue 14096 did ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00136-6
更新日期:2003-07-01 00:00:00
abstract::In the bacterium Escherichia coli, H-NS-(H1, H1a) is a heat-stable protein with a molecular mass of 15.5 kDa involved in nucleoid organisation and gene regulation linked to certain signal transduction pathways. We have shown that, following addition of preparations of everted inner membrane vesicles, heat-stable cleav...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)80025-9
更新日期:1997-06-01 00:00:00
abstract::(-)-Epigallocatechin-3-gallate (EGCG), a well-known chemopreventive factor, induces cancer cells undergoing apoptosis. Over the last several years, we have shown that the mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) functions as an antioxidant and anti-apoptotic protein by supplying NADPH to antioxi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.06.025
更新日期:2011-10-01 00:00:00
abstract::The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80316-7
更新日期:1977-01-01 00:00:00
abstract::Porcine thyroid cells in primary cultures form either monolayers or, when they are stimulated by thyrotropin (TSH), follicles. This system, monolayer-follicle associated cell culture, determines two morphofunctional states of the thyroid cell in vitro. These cells divide, when grown in monolayer. In this article we de...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80367-1
更新日期:1980-01-01 00:00:00
abstract::Apolipoprotein E (apoE) has been genetically linked to late-onset Alzheimer's disease. From the three common alleles (epsilon2, epsilon3 and epsilon4), epsilon4 has been suggested to promote amyloid beta (Ass) plaque fibrillation, one hallmark of Alzheimer's disease. It has been demonstrated that altered lipid content...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.10.007
更新日期:2006-05-01 00:00:00
abstract::Interleukin-17 (IL-17) is produced exclusively by activated T cells and neutrophils, and stimulates osteoclastic bone resorption via osteoblasts by inducing the expression of "receptor activator of NF-kappaB (RANK) ligand" (RANKL). However, the direct effects of IL-17 on the differentiation of osteoclast precursors in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.12.011
更新日期:2010-04-01 00:00:00
abstract::We isolated and purified the main lipids of the rat sciatic nerve. After methanolysis, fatty acids were isolated and purified by thin layer chromatography, and studied by gas chromatography. C 16 and C 18 fatty acids are the most abundant. Among the long-chain fatty acids, arachidonic acid (20:4) is present in some li...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(78)80202-8
更新日期:1978-01-01 00:00:00
abstract::Anaerobiospirillum succiniciproducens phosphoenolpyruvate (PEP) carboxykinase catalyses the reversible metal-dependent formation of oxaloacetate (OAA) and ATP from PEP, ADP and CO(2). Mutations of PEP carboxykinase have been constructed where the residues His(225) and Asp(263), two residues of the enzyme's putative Mn...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2003.10.013
更新日期:2004-01-01 00:00:00
abstract::The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90006-x
更新日期:1994-01-01 00:00:00
abstract::The Golgi apparatus serves as the major site of glycosylation reactions. Nucleotide sugars which are substrates of the Golgi localized glycosyltransferases are synthesized in the cytoplasm (cell nucleus in case of CMP-sialic acid) and must be transported into the compartment lumen. This transport function is carried o...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(01)01322-0
更新日期:2001-08-01 00:00:00
abstract::Parkinson's disease (PD), a neurodegenerative disorder, is characterized by a loss of dopaminergic neurons in the substantia nigra (SN) of the brain and it is well known that the pathogenesis of PD is related to a number of risk factors including oxidative stress. Antioxidant 1 (ATOX1) protein plays a crucial role in ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.10.010
更新日期:2019-01-01 00:00:00
abstract::MicroRNAs (miRNAs) play profound roles in plant growth and development by regulating gene expression. Tetraploid plants often have better physical characteristics and stress tolerance than their diploid progenitors, but the role of miRNAs in this superiority is unclear. Paulownia tomentosa, (Paulowniaceae) is attracti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.02.008
更新日期:2014-07-01 00:00:00
abstract::The termination of protein synthesis in bacteria requires codon-specific polypeptide release factors RF-1 (UAG/UAA specific) and RF-2 (UGA/UAA specific). We have proposed that release factors mimic tRNA and recognize the stop codon for polypeptide release (Nakamura et al (1996) Cell 87, 147-150). In contrast to the te...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)86715-6
更新日期:1996-01-01 00:00:00
abstract::Cell-to-cell movement of Poa semilatent virus (genus Hordeivirus) in infected plants is mediated by three viral 'triple gene block' (TGB) proteins. One of those termed TGBp3 is an integral membrane protein essential for intracellular transport of other TGB proteins and viral genomic RNA to plasmodesmata. TGBp3 targeti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.01.002
更新日期:2011-04-01 00:00:00
abstract::1)Individual monosaccharides (uronic acids and aminosugars) have been purified following specific hydrolysis of the mucopolysaccharides from new born rat skin (hyaluronic acid, heparin + heparan sulfate, chondroitin sulfate A, B and C), after incubation with [U14C]-glucose under various conditions and for varying incu...
journal_title:Biochimie
pub_type: 杂志文章
doi:
更新日期:1975-11-01 00:00:00
abstract::Stereospecific hydrogenations according to the general scheme [formula: see text] are of interest from a preparative and mechanistic point of view. Proteus mirabilis is suitable for the hydrogenation of a-keto-acids to R-hydroxy-acids, and a Clostridium strain for the reduction of enoates. Both have been immobilized i...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80162-3
更新日期:1980-01-01 00:00:00
abstract::Despite its biological importance, iron is a pro-oxidant element and its accumulation results in tissue injury. Iron overload diseases such as thalassemia and hereditary hemochromatosis are commonly associated with liver tissue injury. Glutamyl cysteine (GC) is a dipeptide with antioxidant properties owing to its cyst...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.06.006
更新日期:2015-08-01 00:00:00
abstract::Mitochondrial membranes are essential for the good functioning of the organelle. For instance, the inner mitochondrial membrane contains the oxidative phosphorylation system that permits ATP synthesis. Phospholipids environment and especially cardiolipin are crucial for the mitochondrial energy metabolism. Indeed, car...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.07.009
更新日期:2013-01-01 00:00:00
abstract::Sialic acid, a nine-carbon sugar acid usually is present in the non-reducing terminal position of free oligosaccharides and glycoconjugates. Sialylated conjugates in mammals perform important roles in cellular recognition, signaling, host-pathogen interaction and neuronal development. Metabolism of sialylated conjugat...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2011.06.002
更新日期:2011-10-01 00:00:00
abstract::The measurement and potential technological significance of in vivo missense errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the alpha subu...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(91)90192-4
更新日期:1991-12-01 00:00:00
abstract::Skin aging is characterized by different features including wrinkling, atrophy of the dermis and loss of elasticity associated with damage to the extracellular matrix protein elastin. The aim of this study was to investigate the aging process of skin elastin at the molecular level by evaluating the influence of intrin...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2016.08.010
更新日期:2016-09-01 00:00:00