当前位置： SCI文献检索 > BIOCHIMIE期刊下所有文献 > The application of nuclear magnetic resonance spectroscopy to the study of natural and model membranes.

The application of nuclear magnetic resonance spectroscopy to the study of natural and model membranes.

Abstract：

:This review article outlines some potentials and limits of the recent application of high resolution Nuclear Magnetic Resonance technique--coupled to the Fourier transformation methods--to the study of biological membranes. Molecular arrangement and dynamical structure characters can be assessed at the level of individual chemical groups in lipid bilayer regions of natural and model membranes, through the determination of physical parameters like chemical shifts, spin-lattice (T1) and spin-spin (T2) nuclear magnetic relaxation times. The results of some significant experiments carried out on single-wall lecithin vesicles as well as on intact natural membranes, are summarized and discussed. Useful information can be obtained on the lipid fatty-acid chains thermal transition, by comparing two lecithin vesicles of the same size, formed by the same host lecithin, but incorporated with different molecular components. In particular, T1 and T2 measurements, interpreted in terms of a two- (or more-) correlation time theoretical models, are able to demonstrate different degrees of motional anisotropy in bilayers formed by mixed lecithins or by mixtures of lecithin and fatty acids, possessing moderately different chain lengths [13]. Chromophore-containing molecules, like chlorophyll [12] or fluorescent probes [14] can be located, within few Angstroms, in a lipid bilayer through proton chemical shift measurements; in addition the perturbation of the lipid membrane structure, as induced by the incorporated probe, is assessed mainly in terms of the intramolecular dynamical structure of the host lecithin molecules, by means of T1 and linewidth studies. The comparison of the n.m.r. relaxation behaviour in intact membranes and in vesicles formed by their extracted lipids may, finally, provide indirect information on the lipid-protein intermolecular interactions and relative mobility, besides indicating the intramolecular mobility characters of the lipid bilayer regions of the membrane.

journal_name

Biochimie

journal_title

Biochimie

authors

Podo F

doi

10.1016/s0300-9084(75)80333-6

keywords:

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

461-9

issue

4

eissn

0300-9084

issn

1638-6183

pii

S0300-9084(75)80333-6

journal_volume

57

pub_type

杂志文章,评审

相关文献

BIOCHIMIE文献大全
  • Diacylglycerol kinase δ controls down-regulation of cyclin D1 for C2C12 myogenic differentiation.

    abstract：:Diacylglycerol kinase (DGK) is a lipid-metabolizing enzyme that phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). DGKδ is highly expressed in the skeletal muscle, and a decrease in DGKδ expression increases the severity of type 2 diabetes. However, the role of DGKδ in myogenic differentiation is st...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2018.05.017

    authors： Sakai H,Murakami C,Matsumoto KI,Urano T,Sakane F

    更新日期：2018-08-01 00:00:00

  • Catabolism of arylglycerol-beta-aryl ethers lignin model compounds by Pseudomonas cepacia 122.

    abstract：:Pseudomonas cepacia 122 can grow on several lignin model compounds including the arylglycerol-beta-aryl ethers guaiacylglycerol-beta-coniferyl ether and guaiacylglycerol-beta-guaiacyl ether. Non-phenolic lignin model compounds are not degraded by this bacterium. The enzyme system catalyzing guaiacylglycerol-beta-guaia...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(85)80047-x

    authors： Odier E,Rolando C

    更新日期：1985-02-01 00:00:00

  • Natural mutations lead to enhanced proteasomal degradation of human Ncb5or, a novel flavoheme reductase.

    abstract：:NADH cytochrome b5 oxidoreductase (Ncb5or) protects β-cells against oxidative stress and lipotoxicity. The predominant phenotype of lean Ncb5or-null mouse is insulin-dependent diabetes due to β-cell death. This suggests the putative role of NCB5OR polymorphism in human diabetes. Therefore, we aimed to investigate the ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2013.03.004

    authors： Kálmán FS,Lizák B,Nagy SK,Mészáros T,Zámbó V,Mandl J,Csala M,Kereszturi E

    更新日期：2013-07-01 00:00:00

  • Protein l-isoaspartyl-O-methyltransferase of Vibrio cholerae: interaction with cofactors and effect of osmolytes on unfolding.

    abstract：:Protein l-isoaspartyl-O-methyltransferase (PIMT) is an ubiquitous enzyme widely distributed in cells and plays a role in the repair of deamidated and isomerized proteins. In this study, we show that this enzyme is present in cytosolic extract of Vibrio cholerae, an enteric pathogenic Gram-negative bacterium and is enz...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2012.12.013

    authors： Chatterjee T,Pal A,Chakravarty D,Dey S,Saha RP,Chakrabarti P

    更新日期：2013-04-01 00:00:00

  • Activities of pork muscle proteases in model cured meat systems.

    abstract：:The effect of curing agents (salt, nitrate, ascorbic acid and glucose) and processing parameters (pH, water activity and drying and cooking temperatures) on pork muscle cathepsins B, D, H and L as well as leucyl, arginyl and tyrosyl hydrolysing activities is reported. Salt (60 g/l) showed a powerful inhibitory effect,...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(92)90128-2

    authors： Toldrá F,Rico E,Flores J

    更新日期：1992-03-01 00:00:00

  • Two-dimensional 1H NMR spectroscopy of normal and pathological human plasma: complete water suppression and further assignment of resonances.

    abstract：:Two-dimensional J-resolved and correlated 1H NMR spectra with complete water suppression have been obtained to further characterize a metabolic pattern for normal and pathological human plasma samples. 1H COSY spectra have been recorded on plasma from 12 patients with cancer in order to check for the possible presence...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(92)90075-p

    authors： Kriat M,Confort-Gouny S,Vion-Dury J,Viout P,Cozzone PJ

    更新日期：1992-09-01 00:00:00

  • Low enantioselectivities of human deoxycytidine kinase and human deoxyguanosine kinase with respect to 2'-deoxyadenosine, 2'-deoxyguanosine and their analogs.

    abstract：:The antiviral activity of L-nucleoside analogs depends in part on the enantioselectivity of nucleoside kinases which catalyse their monophosphorylation. The substrate properties of human recombinant deoxycytidine kinase (dCK) and human recombinant deoxyguanosine kinase (dGK) with respect to L-adenosine and L-guanosine...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(99)00331-4

    authors： Gaubert G,Gosselin G,Boudou V,Imbach JL,Eriksson S,Maury G

    更新日期：1999-11-01 00:00:00

  • Influence of an anion-binding site in the stabilization of halophilic malate dehydrogenase from Haloarcula marismortui.

    abstract：:Halophilic proteins have evolved to be soluble, stable and active in high salt concentration. Crystallographic studies have shown that surface enrichment by acidic amino acids is a common structural feature of halophilic proteins. In addition, ion-binding sites have also been observed in most of the cases. The role of...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2007.03.008

    authors： Madern D,Ebel C

    更新日期：2007-08-01 00:00:00

  • Calcium and spermine interaction with phospholipid bilayers: a 15N NMR study.

    abstract：:The binding of Ca2+ to membrane models composed of diplamitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE) 15N-labeled in the polar head group was investigated at pH 8.5 and pH 9.4 by 15N-NMR spectroscopy. Both phospholipids exhibit a decrease in the chemical shift anisotropy, indicating c...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(89)90143-0

    authors： Akoka S,Tellier C

    更新日期：1989-01-01 00:00:00

  • Denitrification and nitrite reduction: Pseudomonas aeruginosa nitrite-reductase.

    abstract：:Present knowledge of the different enzymatic steps of the denitrification chains in various bacteria, particularly Paracoccus denitrificans and Pseudomonas aeruginosa has been briefly reviewed. The question whether nitric oxide (NO), nitrous oxide (N2O) and other nitrogen derivatives are obligatory intermediates has b...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/0300-9084(84)90005-1

    authors： Henry Y,Bessières P

    更新日期：1984-04-01 00:00:00

  • A comparative Raman spectroscopic study of cholinesterases.

    abstract：:We report Raman spectra of various cholinesterases: lytic tetrameric forms (G4) obtained by tryptic digestion of asymmetric acetylcholinesterase (AChE) from Torpedo californica and Electrophorus electricus, a PI-PLC-treated dimeric form (G2) of AChE from T marmorata, and the soluble tetrameric form (G4) of butyrylchol...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(91)90167-y

    authors： Aslanian D,Grof P,Bon S,Masson P,Négrerie M,Chatel JM,Balkanski M,Taylor P,Massoulié J

    更新日期：1991-11-01 00:00:00

  • Three-dimensional structure of an unusual Kunitz (STI) type trypsin inhibitor from Copaifera langsdorffii.

    abstract：:The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2004.03.004

    authors： Krauchenco S,Nagem RA,da Silva JA,Marangoni S,Polikarpov I

    更新日期：2004-03-01 00:00:00

  • Crystal structure of the NADP-dependent mannitol dehydrogenase from Cladosporium herbarum: Implications for oligomerisation and catalysis.

    abstract：:The ascomycete Cladosporium herbarum is a prominent fungal inducer of Type I allergy. The only major allergen identified so far is Cla h 8, a NADP-dependent mannitol dehydrogenase (MtDH). MtDH, a cytoplasmic protein of 28.5kDa, belongs to the Short chain Dehydrogenases/Reductases (SDR), acting as a NADP-dependent oxid...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2010.04.012

    authors： Nüss D,Goettig P,Magler I,Denk U,Breitenbach M,Schneider PB,Brandstetter H,Simon-Nobbe B

    更新日期：2010-08-01 00:00:00

  • Pterocarpans induce tumor cell death through persistent mitotic arrest during prometaphase.

    abstract：:Pterocarpans, a family of isoflavonoids found in the diverse Fabaceae, display potent cytotoxic activity over a panel of tumor cell lines, and among those tested, 2,3,9- trimethoxypterocarpan displays the most potent activity. This study evaluates the effects of 2,3,9-trimethoxypterocarpan and its related derivatives ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2014.06.005

    authors： Militão GC,Prado MP,Pessoa C,de Moraes MO,Silveira ER,Lima MA,Veloso PA,Costa-Lotufo LV,Machado-Santelli GM

    更新日期：2014-09-01 00:00:00

  • Biological data integration using Semantic Web technologies.

    abstract：:Current research in biology heavily depends on the availability and efficient use of information. In order to build new knowledge, various sources of biological data must often be combined. Semantic Web technologies, which provide a common framework allowing data to be shared and reused between applications, can be ap...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2008.02.007

    authors： Pasquier C

    更新日期：2008-04-01 00:00:00

  • PPAR gamma, 10 years later.

    abstract：:It is now over 10 years since the discovery of peroxisome proliferator activated receptor gamma (PPAR gamma) and its unique role in adipogenesis. The subsequent identification of PPAR gamma as the target of insulin sensitizing drugs certified this ligand-regulated transcription factor as an exciting link between adipo...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/j.biochi.2004.10.021

    authors： Lazar MA

    更新日期：2005-01-01 00:00:00

  • Origin and evolution of metabolic sub-cellular compartmentalization in eukaryotes.

    abstract：:A high level of subcellular compartmentalization is a hallmark of eukaryotic cells. This intricate internal organization was present already in the common ancestor of all extant eukaryotes, and the determination of the origins and early evolution of the different organelles remains largely elusive. Organellar proteome...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/j.biochi.2015.03.021

    authors： Gabaldón T,Pittis AA

    更新日期：2015-12-01 00:00:00

  • Amino acid sequences of cytochromes c2 and c' from the moderately halophilic purple phototrophic bacterium Rhodospirillum salexigens.

    abstract：:Rhodospirillum salexigens is a moderately halophilic purple phototrophic bacterium which grows optimally in 8% NaCl. The amino acid sequences of the two principal soluble cytochromes c have been determined. One of these is a cytochrome c2, similar in size to mitochondrial cytochrome c. While clearly of the same sequen...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(94)90135-x

    authors： Ambler RP,Daniel M,Meyer TE,Kamen MD

    更新日期：1994-01-01 00:00:00

  • LasX, a transcriptional regulator of the lactocin S biosynthetic genes in Lactobacillus sakei L45, acts both as an activator and a repressor.

    abstract：:The 11 kb las locus, present on the 50 kb plasmid pCIM1, specifies the production of the lantibiotic lactocin S in Lactobacillus sakei L45. The gene cluster is organized into two oppositely orientated operons, lasAMNTUVPJW (lasA-W) and lasXY, the former of which contains the biosynthetic, immunity and transport genes....

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/s0300-9084(02)01420-7

    authors： Rawlinson EL,Nes IF,Skaugen M

    更新日期：2002-05-01 00:00:00

  • Effect of the terminal phosphate derivatization of beta- and alpha-oligodeoxynucleotides on their antisense activity in protein biosynthesis, stability and uptake by eucaryotic cells.

    abstract：:Inhibition of polypeptide chain elongation with the mRNA-complementary (antisense) oligonucleotide has been realized through a RNase H independent mechanism. Nuclease resistant complementary non-natural alpha-17-mer oligonucleotide did not inhibit cell-free protein biosynthesis of beta-globin in the wheat germ system ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(92)90089-w

    authors： Boutorine AS,Boiziau C,Le Doan T,Toulmé JJ,Hélène C

    更新日期：1992-05-01 00:00:00

  • [Influence of glyoxylic acid on properties of isolated mitochondria].

    abstract：:Glyoxalate is an effector of oxidative phosphorylation in isolated mitochondria : it slows down State 3 but does not affect State 4 respiration. This report presents the findings of our study on the mechanism of action of glyoxalate ; these findings are listed below. The inhibition of Stage 3 respiration by glyoxalate...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(75)80145-3

    authors： Lucas M,Pons AM

    更新日期：1975-01-01 00:00:00

  • [Modes of action of a shortening system for erucic acid at the mitochondrial level of rat liver].

    abstract：:In this work, were studied the conditions of erucic acid (cis-docosenoic, n-9) shortening by using Rat liver mitochondrial preparations which were incubated in vitro with [14-14C] erucic acid (22:1), with inhibitors of the respiratory chain (rotenone, cyanide) or not, with activators of either the shortening reaction ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/s0300-9084(82)80352-0

    authors： Clouet P,Henninger C,Bezard J

    更新日期：1982-10-01 00:00:00

  • Regulation of CIRL-1 proteolysis and trafficking.

    abstract：:Calcium-independent receptor of alpha-latrotoxin (CIRL-1) is an adhesion G protein-coupled receptor implicated in the regulation of exocytosis. CIRL-1 biosynthesis involves constitutive proteolytic processing that takes place in the endoplasmic reticulum, requires the receptor's GPS domain, and yields heterologous two...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2010.01.015

    authors： Deyev IE,Petrenko AG

    更新日期：2010-04-01 00:00:00

  • Bacterial cell wall macroamphiphiles: pathogen-/microbe-associated molecular patterns detected by mammalian innate immune system.

    abstract：:Innate immune system is the first line of host defense against invading microorganisms. It relies on a limited number of germline-encoded pattern recognition receptors that recognize conserved molecular structures of microbes, referred to as pathogen-/microbe-associated molecular patterns (PAMPs/MAMPs). Bacterial cell...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/j.biochi.2012.06.007

    authors： Ray A,Cot M,Puzo G,Gilleron M,Nigou J

    更新日期：2013-01-01 00:00:00

  • Crystal structures of triosephosphate isomerase from methicillin resistant Staphylococcus aureus MRSA252 provide structural insights into novel modes of ligand binding and unique conformations of catalytic loop.

    abstract：:Staphylococcus aureus is one of the most dreaded pathogens worldwide and emergence of notorious antibiotic resistant strains have further exacerbated the present scenario. The glycolytic enzyme, triosephosphate isomerase (TIM) is one of the cell envelope proteins of the coccus and is involved in biofilm formation. It ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2012.07.001

    authors： Mukherjee S,Roychowdhury A,Dutta D,Das AK

    更新日期：2012-12-01 00:00:00

  • Chromosomal instability and alteration of telomere repeat sequences.

    abstract：:The very end of the chromosome is called the telomere and is composed of DNA repeat sequences and associated proteins. Genetic and biochemical analyses of this complex, the telosome, lead to the hypothesis that transcription and DNA replication are submitted to position effects mediated by the telomere proximity. Telo...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/0300-9084(96)88201-0

    authors： Pommier JP,Lebeau J,Ducray C,Sabatier L

    更新日期：1995-01-01 00:00:00

  • An empirical energy function for structural assessment of protein transmembrane domains.

    abstract：:Knowing the structure of a protein is essential to characterize its function and mechanism at the molecular level. Despite major advances in solving structures experimentally, most membrane protein native conformations remain unknown. This lack of available structures, along with the physical constraints imposed by th...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2015.05.018

    authors： Postic G,Ghouzam Y,Gelly JC

    更新日期：2015-08-01 00:00:00

  • Contact-dependent inhibition of growth of normal diploid human fibroblasts by plasma membrane glycoproteins.

    abstract：:Homeostasis in vivo is maintained by a highly complex network of positive and negative signals. At the cellular level, this regulatory microenvironment can be divided, in a simplified fashion, into two major compartments: the humoral compartment, including compounds such as hormones, growth factors and nutrients, and ...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/0300-9084(88)90301-x

    authors： Wieser RJ,Janik-Schmitt B,Renauer D,Schäfer A,Heck R,Oesch F

    更新日期：1988-11-01 00:00:00

  • Impairment of the Pin1/E2F1 axis in the anti-proliferative effect of bortezomib in hepatocellular carcinoma cells.

    abstract：BACKGROUND:The modest efficacy of available therapies for Hepatocellular carcinoma (HCC) indicates the need to develop novel therapeutic approaches. For the proteasome inhibitor Bortezomib (BZB), potentially attractive for HCC treatment, the mechanism of action is largely unknown. The BZB effect on E2Fs and the E2Fs co...

    journal_title：Biochimie

    pub_type： 杂志文章

    doi：10.1016/j.biochi.2015.02.015

    authors： Farra R,Dapas B,Baiz D,Tonon F,Chiaretti S,Del Sal G,Rustighi A,Elvassore N,Pozzato G,Grassi M,Grassi G

    更新日期：2015-05-01 00:00:00

  • A multi-functional enzyme with RNA polymerase and RNase activities: molecular anatomy of influenza virus RNA polymerase.

    abstract：:Influenza virus-associated RNA polymerase is composed of one molecule each of three viral P proteins and carries the complete activity of capped RNA-primed vRNA-directed transcription. The RNA polymerase holoenzyme also carries capped RNA endonuclease to generate capped oligonucleotide primers for transcription and 3'...

    journal_title：Biochimie

    pub_type： 杂志文章,评审

    doi：10.1016/s0300-9084(97)86735-1

    authors： Ishihama A

    更新日期：1996-01-01 00:00:00