Hypermethioninemia provokes oxidative damage and histological changes in liver of rats.

abstract：:The field of immunometabolism has come a long way in the past decade, leading to the emergence of a new role for white adipose tissue (WAT) that is now recognized to stand at the junction of immune and metabolic regulations. Interestingly, a crucial role of the abundant and heterogeneous immune population present in W...

journal_title：Biochimie

authors： Cousin B,Casteilla L,Laharrague P,Luche E,Lorsignol A,Cuminetti V,Paupert J

更新日期：2016-05-01 00:00:00

abstract：:The effects on intra- and extracellular pH of two polyenic derivatives of amphotericin B, N-fructosyl amphotericin B and N-fructosyl amphotericin B methyl-ester, were tested on HL-60 promyelocytic leukemia cells. Both derivatives raised the internal pH and reduced the external pH in weakly buffered medium. These resul...

journal_title：Biochimie

authors： Charretier E,Wietzerbin J

更新日期：1989-01-01 00:00:00

abstract：:Protein 3D structures support their biological functions. As the number of protein structures is negligible in regards to the number of available protein sequences, prediction methodologies relying only on protein sequences are essential tools. In this field, protein secondary structure prediction (PSSPs) is a mature ...

journal_title：Biochimie

authors： de Brevern AG

更新日期：2020-12-01 00:00:00

abstract：:Structural studies on various electron transfer complexes involving the tetrahemic cytochrome c3 provided evidence that one of the hemes (heme 4) is the interacting site on the molecule. The reactivity of this particular heme is allocated to the positive charges found around the heme group which are strongly involved ...

journal_title：Biochimie

authors： Guerlesquin F,Dolla A,Bruschi M

更新日期：1994-01-01 00:00:00

abstract：:Starting from total pancreatic mRNAs, turkey pancreatic lipase (TPL) cDNA was synthesized by RT-PCR and cloned into the PGEM-T vector. Amino acid sequence of the TPL is compared to that of human pancreatic lipase (HPL). A 3-D structure model of TPL was built using the 3-D structure of HPL as template, given the high a...

journal_title：Biochimie

authors： Fendri A,Frikha F,Miled N,Gargouri Y

更新日期：2006-10-01 00:00:00

abstract：:The repressible Saccharomyces cerevisiae acid phosphatase (APase) coded by the PHO5 gene is a cell wall protein that follows the yeast secretory pathway. We had previously described the in vivo fate of a multicopy plasmid-encoded modified protein, lacking 15 out of 17 signal peptide amino acids. This modified protein ...

journal_title：Biochimie

authors： Silve S,Volland C,Hinnen A,Haguenauer-Tsapis R

更新日期：1990-02-01 00:00:00

abstract：:The presence of marker genes encoding antibiotic or herbicide resistances in genetically modified plants poses a number of problems. Various techniques are under development for the removal of unwanted marker genes, while leaving required transgenes in place. The aim of this brief review is to describe the principal m...

journal_title：Biochimie

authors： Scutt CP,Zubko E,Meyer P

更新日期：2002-11-01 00:00:00

abstract：:Inducing brown adipocytes in white adipose tissues is a promising target to combat obesity and its related disorders in human beings. This goal has been especially encouraged by new important discoveries of human brown adipose tissues. The accumulating evidence confirms the presence of active brown adipocytes, not onl...

journal_title：Biochimie

authors： Chu DT,Gawronska-Kozak B

更新日期：2017-07-01 00:00:00

abstract：:The hypothesis is proposed that pre-biotic bacterial cell(s) and the first cells capable of growth/division did not require a cytoplasmic membrane. A gel-like microscopic structure less than a cubic micrometer may have had a dual role as both an ancient pre-cytoplasm and a boundary layer to the higher-entropy external...

journal_title：Biochimie

authors： Trevors JT,Pollack GH

更新日期：2012-01-01 00:00:00

abstract：:Identification of proteoglycans in two human malignant mesothelioma cell lines, one with epithelial differentiation and the other with fibroblast-like phenotype, and the effects of epidermal (EGF), insulin-like (IGF-I) and platelet-derived (PDGF-BB) growth factors on the synthesis of hyaluronan (HA) and proteoglycans ...

journal_title：Biochimie

authors： Syrokou A,Tzanakakis GN,Hjerpe A,Karamanos NK

更新日期：1999-07-01 00:00:00

abstract：:The Escherichia coli Fis protein is a dimeric DNA-binding protein whose specific binding sites share a weak consensus sequence. Use of the gel retardation technique indicates that binding of Fis on a linear DNA fragment leads to the formation of a ladder of defined retarded complexes, independently of the presence of ...

journal_title：Biochimie

authors： Bétermier M,Galas DJ,Chandler M

更新日期：1994-01-01 00:00:00

abstract：:It has been shown that two Proteus morganii strains produce an inducible cephalosporinase. No significative difference was shown between them: they present the same isoelectric poit: 8,3 and very similar kinetic constants. The parameter tau, proportional to the half life of antibiotic at low concentration, in presence...

journal_title：Biochimie

authors： Labia R,Guionie M,Fabre C

更新日期：1976-11-13 00:00:00

abstract：:Thraustochytrium is a marine protist producing a specific profile of nutritionally important fatty acids, including very long chain polyunsaturated fatty acids (VLCPUFAs) docosahexaenoic acid (DHA, 22:6n-3), even chain saturated fatty acids (SFAs) palmitic acid (16:0), and odd chain SFAs pentadecanoic acid (15:0). To ...

journal_title：Biochimie

authors： Zhao X,Qiu X

更新日期：2018-01-01 00:00:00

abstract：:We are here reporting that calpain-1 is a constitutive component of a distinct lipid raft/caveolin-1 microdomain isolated from bEnd5 cells in association with endothelial nitric oxide synthase (eNOS) and heat shock protein 90 (HSP90). Perturbations in intracellular calcium concentration by Ca2+-ionophore A23187 or pro...

journal_title：Biochimie

authors： Martines A,Stifanese R,Faelli EL,Perasso L,Melloni I,Ruggeri P,Averna M

更新日期：2017-02-01 00:00:00

abstract：:Long-term treatment with ambroxol (ABX), a bronchial expectorant, was found to prevent acute exacerbation of chronic obstructive pulmonary disease (AECOPD). The underlying mechanism remains unclear. To address this, we have investigated the effect of ABX on critical antioxidant proteins thioredoxin (Trx) and thioredox...

journal_title：Biochimie

authors： Huang J,Xu J,Tian L,Zhong L

更新日期：2014-02-01 00:00:00

abstract：:Directed mutagenesis has been used to study the nicotinamide subsite of the glycolytic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Residue Asn313 is involved together with the carboxyamide moiety of the nicotinamide ring in a complex network of hydrogen bonding interactions which fix the position o...

journal_title：Biochimie

authors： Corbier C,Mougin A,Mely Y,Adolph HW,Zeppezauer M,Gerard D,Wonacott A,Branlant G

更新日期：1990-08-01 00:00:00

abstract：:The Gag-encoded nucleocapsid protein NCp7 (72 amino acids) from HIV-1, the regulatory protein, Vpr (96 amino acids) and numerous derivatives have been synthesized by solid phase method and their structures determined by 2D NMR. In NCp7, the two highly folded zinc fingers of the Cx2Cx4Hx4C type are in close spacial pro...

journal_title：Biochimie

authors： Roques BP,Morellet N,de Rocquigny H,Déméné H,Schueler W,Jullian N

更新日期：1997-11-01 00:00:00

abstract：:We present an approach for designing a knowledge-based system, called Sequence Acquisition In Context (SAIC), that will be able to cooperate with a biologist in the analysis of DNA sequences. The main task of the system is the acquisition of the expert knowledge that the biologist uses for solving ambiguities from gel...

journal_title：Biochimie

authors： Brézillon PJ,Zaraté P,Saci F

更新日期：1993-01-01 00:00:00

abstract：:Sequence analysis of the Lutzomyia (Lu.) ayacuchensis salivary gland cDNA library identified a short peptide containing an RGD (Arg-Gly-Asp) sequence flanked by two cysteine residues in the C-terminal end as the most abundant transcript. In the present study, a recombinant protein of the RGD-containing peptide, design...

journal_title：Biochimie

authors： Kato H,Gomez EA,Fujita M,Ishimaru Y,Uezato H,Mimori T,Iwata H,Hashiguchi Y

更新日期：2015-05-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris is often used as an organism for the heterologous expression of proteins and has been used already for production of a number of glycosyltransferases involved in the biosynthesis of N- and O-linked oligosaccharides. In our recent studies, we have examined the expression in P. p...

journal_title：Biochimie

authors： Bencúrová M,Rendić D,Fabini G,Kopecky EM,Altmann F,Wilson IB

更新日期：2003-03-01 00:00:00

abstract：:In this contribution, we have studied the dynamics of electron transfer (ET) of a flavoprotein to the bound cofactor, an important metabolic process, in a model molecular/macromolecular crowding environments. Vitamin B(2) (riboflavin, Rf) and riboflavin binding protein (RBP) are used as model cofactor and flavoprotein...

journal_title：Biochimie

authors： Rakshit S,Saha R,Verma PK,Mitra RK,Pal SK

更新日期：2012-12-01 00:00:00

abstract：:gamma-Aminobutyraldehyde dehydrogenase from Escherichia coli K-12 has been purified and characterized from cell mutants able to grow in putrescine as the sole carbon and nitrogen source. The enzyme has an Mr of 195,000 +/- 10,000 in its dimeric form with an Mr of 95,000 +/- 1,000 for each subunit, a pH optimum at 5.4 ...

journal_title：Biochimie

authors： Prieto MI,Martin J,Balaña-Fouce R,Garrido-Pertierra A

更新日期：1987-11-01 00:00:00

abstract：:The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central ner...

journal_title：Biochimie

authors： Sarbu M,Raab S,Henderson L,Fabris D,Vukelić Ž,Clemmer DE,Zamfir AD

更新日期：2020-03-01 00:00:00

abstract：:Apolipoprotein E (apoE) has been genetically linked to late-onset Alzheimer's disease. From the three common alleles (epsilon2, epsilon3 and epsilon4), epsilon4 has been suggested to promote amyloid beta (Ass) plaque fibrillation, one hallmark of Alzheimer's disease. It has been demonstrated that altered lipid content...

journal_title：Biochimie

authors： Rapp A,Gmeiner B,Hüttinger M

更新日期：2006-05-01 00:00:00

abstract：:Cell sterol supply is subjected to tight negative feedback regulation through the SREBP pathway. Upon cholesterol depletion, SREBP transcription factors become activated by cleavage of a membrane bound precursor form, which stimulates the expression of the genes encoding proteins of the cholesterol synthesis pathway. ...

journal_title：Biochimie

authors： Robichon C,Dugail I

更新日期：2007-02-01 00:00:00

abstract：:The number of proteins known to contain collagen-like triple helical domains is rapidly increasing. The functions of these domains are to provide molecular rods that separate spatially non-triple helical domains with varied properties and structures and to permit lateral interactions between molecules. Two-thirds of t...

journal_title：Biochimie

authors： van der Rest M,Garrone R

更新日期：1990-06-01 00:00:00

abstract：:A discriminating system capable of recognizing the oxygenated sterols was investigated in human lymphocytes. After labelling entire cells with 25-hydroxy [3H] cholesterol (10 nM) the cytosol was ultracentrifuged on a linear sucrose density gradient. Bound 25-hydroxy [3H] cholesterol was located in a single peak with a...

journal_title：Biochimie

authors： Defay RE,Astruc ME,Roussillon S,Descomps B,Crastes De Paulet A

更新日期：1982-05-01 00:00:00

abstract：:The present study was conducted on CD4(+) T cells, isolated from wild type (WT) and PPARalpha(null) mice, in order to assess the mechanism of action of docosahexaenoic acid (DHA), an n-3 fatty acid, in the modulation of two transcription factors, i.e., T-bet and GATA-3, implicated in T-cell differentiation towards, re...

journal_title：Biochimie

authors： Attakpa E,Hichami A,Simonin AM,Sansón EG,Dramane KL,Khan NA

更新日期：2009-11-01 00:00:00

abstract：:The complete nucleotide sequence of a nerve growth factor precursor from Bothrops jararacussu snake (Bj-NGF) was determined by DNA sequencing of a clone from cDNA library prepared from the poly(A) + RNA of the venom gland of B. jararacussu. cDNA encoding Bj-NGF precursor contained 723 bp in length, which encoded a pre...

journal_title：Biochimie

authors： Kashima S,Soares AM,Roberto PG,Pereira JO,Astolfi-Filho S,Cintra AO,Fontes MR,Giglio JR,de Castro França S

更新日期：2002-07-01 00:00:00

abstract：:Translational control is a common mechanism used to regulate gene expression and occur in bacteria to mammals. Typically in translational control, an RNA-binding protein binds to a unique sequence in the mRNA to regulate protein synthesis by the ribosomes. Alternatively, a protein may bind to or modify a translation f...

journal_title：Biochimie

authors： Chen E,Joseph S

更新日期：2015-07-01 00:00:00