Abstract:
:Sequence analysis of the Lutzomyia (Lu.) ayacuchensis salivary gland cDNA library identified a short peptide containing an RGD (Arg-Gly-Asp) sequence flanked by two cysteine residues in the C-terminal end as the most abundant transcript. In the present study, a recombinant protein of the RGD-containing peptide, designated ayadualin, was expressed in Escherichia coli and its activity was characterized. Ayadualin inhibited both collagen and ADP-induced platelet aggregations by interfering with the binding of integrin αIIbβ3 to fibrinogen. The RGD sequence and cysteine residues located on both sides of the RGD sequence were essential for the inhibitory action. Moreover, ayadualin efficiently inhibited the intrinsic blood coagulation pathway irrespective of the RGD sequence. Measuring the enzymatic activity of coagulation factors using chromogenic substrates revealed that ayadualin efficiently inhibited factor XIIa (FXIIa) activity in a dose-dependent manner. In addition, pre-incubation of ayadualin with FXII inhibited FXIIa activity, while activated FXIIa was not affected by ayadualin, indicating that ayadualin inhibits the activation of FXII, but not enzymatic activity of FXIIa. These results indicated that ayadualin plays an important role in the blood feeding of Lu. ayacuchensis by inhibiting host hemostasis via dual mechanisms.
journal_name
Biochimiejournal_title
Biochimieauthors
Kato H,Gomez EA,Fujita M,Ishimaru Y,Uezato H,Mimori T,Iwata H,Hashiguchi Ydoi
10.1016/j.biochi.2015.02.011subject
Has Abstractpub_date
2015-05-01 00:00:00pages
49-56eissn
0300-9084issn
1638-6183pii
S0300-9084(15)00046-2journal_volume
112pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Rhodospirillum salexigens is a moderately halophilic purple phototrophic bacterium which grows optimally in 8% NaCl. The amino acid sequences of the two principal soluble cytochromes c have been determined. One of these is a cytochrome c2, similar in size to mitochondrial cytochrome c. While clearly of the same sequen...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90135-x
更新日期:1994-01-01 00:00:00
abstract::Phosphorus metabolites and intracellular pH have been examined in the slime mold Dictyostelium discoideum by non-destructive 31P-NMR measurements. In a spectrum from a suspension of aerobic amoebae, the major peaks are inorganic phosphate, nucleotide di- and triphosphates. In the corresponding perchloric acid extract,...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(86)80077-3
更新日期:1986-12-01 00:00:00
abstract::Injection of 3,5,3' L-triiodothyronine (15 micrograms/100 g) induces a biphasic enhancement of rat heart ornithine decarboxylase (EC. 4.1.17) activity after 4 and 21 hours. This induction is observed after each daily injection, but to a lesser extent. The properties of partially purified basal enzyme and induced enzym...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80383-0
更新日期:1982-11-01 00:00:00
abstract::A lignin model compound, named in short guaiagylglycerol beta-guaiacyl ether (GGE), contains the beta-0-4 ether linkage that is common in the chemical structure of lignin. A Pseudomonas sp. (GU5) had been isolated as an organism able to grow with GGE as the sole source of carbon and energy. When grown on vanillate, th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80292-3
更新日期:1985-09-01 00:00:00
abstract::Estrogen plays an essential role in bone formation, and estrogen modulation dysfunction is tightly associated with postmenopausal osteoporosis (PMOP). The underlying mechanisms of estrogen-mediated osteogenic differentiation have not been well defined. In this study, murine bone marrow mesenchymal stem cells were indu...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.01.001
更新日期:2015-03-01 00:00:00
abstract::The reactivity of sonicated phosphatidylcholine-cholesterol liposomes with cholesterol : oxygene oxydoreductase, an enzyme which catalyses the oxidation of the 3 beta hydroxyl group of cholesterol to a ketone group, is compared with that of ternary system phosphatidylcholine-cholesterol-Thesit. Regardless to the phosp...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(79)80205-9
更新日期:1979-01-01 00:00:00
abstract::The Cystic Fibrosis Transmembrane Regulator (CFTR) is a membrane protein whose mutations cause cystic fibrosis, a lethal genetic disease. We performed a molecular dynamic (MD) study of the properties of the nucleotide binding domains (NBD) whose conformational changes, upon ATP binding, are the direct responsible of t...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.01.010
更新日期:2015-04-01 00:00:00
abstract::This paper deals with the discovery of plasmalogen deficiency in the cerebro-hepato-renal (Zellweger) syndrome and discusses how this has led to the development of postnatal and prenatal diagnostic procedures for this and a number of related peroxisomal disorders in man that show a general impairment in the biosynthes...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(93)90076-5
更新日期:1993-01-01 00:00:00
abstract::Carbonic anhydrases (CAs, EC 4.2.1.1) are metalloenzymes found throughout the phylogenetic tree. The β-class carbonic anhydrases (β-CAs) are the predominating class of CAs in plants. Growing evidence underscores the importance of β-CAs in plant immunity and environmental adaptation in addition to their roles in photos...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.10.020
更新日期:2014-02-01 00:00:00
abstract::Isopentenyl adenosine derivatives can be found next to the anticodon (position 37) in tRNA from both the Bacteria and Eucarya domains. These modified nucleosides improve the efficiency of tRNA in translation, can increase and decrease translational fidelity, and make the tRNA less codon context sensitive. In bacteria ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(94)90044-2
更新日期:1994-01-01 00:00:00
abstract::Diacylglycerol kinase (DGK) phosphorylates diacylglycerol to produce phosphatidic acid (PA). The η isozyme of DGK is abundantly expressed in C2C12 myoblasts. However, the role of DGKη in skeletal muscle cells remains unknown. In the present study, we showed that DGKη was downregulated at an early stage of myogenic dif...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.07.018
更新日期:2020-10-01 00:00:00
abstract::Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and e...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.07.025
更新日期:2013-11-01 00:00:00
abstract::Because of the ability of photosensitizers to induce specific photochemical reactions in vivo, leading to cell injury and death, many such molecules have been considered as therapeutic agents. Among them two classes of sensitizers, i.e. furocoumarins (psoralens) and porphyrins, are currently used for the photochemothe...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(86)80103-1
更新日期:1986-06-01 00:00:00
abstract::Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.03.023
更新日期:2015-06-01 00:00:00
abstract::Two-dimensional J-resolved and correlated 1H NMR spectra with complete water suppression have been obtained to further characterize a metabolic pattern for normal and pathological human plasma samples. 1H COSY spectra have been recorded on plasma from 12 patients with cancer in order to check for the possible presence...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(92)90075-p
更新日期:1992-09-01 00:00:00
abstract::The mechanism responsible for creating the division site in the right place at the right time in bacteria is unknown. It has been attributed to the formation of proteolipid domains in the cytoplasmic membrane surrounding the nucleoids. We interpret the growing evidence for this hypothesis by invoking hyperstructures, ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(00)01203-7
更新日期:2001-01-01 00:00:00
abstract::In the present study we evaluated the effect of chronic methionine administration on oxidative stress and biochemical parameters in liver and serum of rats, respectively. We also performed histological analysis in liver. Results showed that hypermethioninemia increased chemiluminescence, carbonyl content and glutathio...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.04.018
更新日期:2009-08-01 00:00:00
abstract::Previous reports on the carbohydrate specificities of Amaranthus caudatus lectin (ACL) and peanut agglutinin (PNA, Arachis hypogea) indicated that they share the same specificity for the Thomsen-Friedenreich (T(alpha), Galbeta1-3GalNAcalpha1-Ser/Thr) glycotope, but differ in monosaccharide binding--GalNAc>Gal (inactiv...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.08.001
更新日期:2008-11-01 00:00:00
abstract::In this work, were studied the conditions of erucic acid (cis-docosenoic, n-9) shortening by using Rat liver mitochondrial preparations which were incubated in vitro with [14-14C] erucic acid (22:1), with inhibitors of the respiratory chain (rotenone, cyanide) or not, with activators of either the shortening reaction ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80352-0
更新日期:1982-10-01 00:00:00
abstract::Polysialylated neural cell adhesion molecule (NCAM) was immunoaffinity-purified from the brains of newborn calves. A degree of polymerization of up to 40 was chromatographically determined for released polysialic acid (PSA) chains. For characterization of N-glycan structures and attachment sites, PSA-NCAM was digested...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00043-9
更新日期:2003-01-01 00:00:00
abstract::Glutathione, being a major intracellular redox regulator has been shown to be implicated in regulation of airway reactivity and inflammation. However, no study so far has investigated the effect of glutathione depletion/repletion during sensitization and challenge phases separately, which could provide an important in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.04.001
更新日期:2014-08-01 00:00:00
abstract::Human telomeres are intrinsically dynamic structures, with multiple biological processes operating to generate substantial length heterogeneity. Processes that operate specifically at the terminus, that include the end-replication problem coupled with C-strand resection, result in gradual telomere erosion with ongoing...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.08.003
更新日期:2008-01-01 00:00:00
abstract::In recent years an increasing number of inherited decreases in man has been identified in which there is an impairment of one or more peroxisomal functions. Sofar 15 different peroxisomal disorders have been identified which can be subdivided into three distinct groups depending upon whether there is a generalized (gr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(93)90087-9
更新日期:1993-01-01 00:00:00
abstract::Adenosine to inosine (A-to-I) modification by the ADAR (adenosine deaminase that acts on RNA) enzymes perform the most common type of RNA editing in metazoans. ADARs use double stranded RNA as substrates but allow interruptions of bulges and loops in the structure. It is well known that these enzymes can use messenger...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.06.002
更新日期:2007-10-01 00:00:00
abstract::Bacterial survive and respond to adverse changes in the environment by regulating gene transcription through two-component regulatory systems. In Salmonella Typhimurium the STM1485 gene expression is induced under low pH (4.5) during replication inside the epithelial host cell, but it is not involved in sensing or res...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.02.005
更新日期:2019-05-01 00:00:00
abstract::Flavodoxins are one domain alpha/beta electron transfer proteins that participate in photosynthetic reactions. All flavodoxins carry a molecule of flavin mononucleotide (FMN), non-covalently bound, that confers redox properties to the protein. There are two structurally distinct flavodoxins, short ones and long flavod...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)88876-8
更新日期:1998-10-01 00:00:00
abstract::At least 6 N-acetylglucosaminyltransferases (GlcNAc-T I, II, III, IV, V and VI) are involved in initiating the synthesis of the various branches found in complex asparagine-linked oligosaccharides (N-glycans), as indicated below: GlcNAc beta 1-6 GlcNAc-T V GlcNAc beta 1-4 GlcNAc-T VI GlcNAc beta 1-2Man alpha 1-6 GlcNA...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90289-1
更新日期:1988-11-01 00:00:00
abstract::The concept of a stem cell subpopulation as understood from normal epithelial tissue or bone marrow function has been extended to our understanding of cancer tissue and is now the target of treatment efforts specifically directed to this subpopulation. In glioblastoma, as well as in other cancers, increased expression...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.11.007
更新日期:2013-04-01 00:00:00
abstract::Alteration of the epigenome is associated with a wide range of human diseases. Therefore, deciphering the pathways that regulate the epigenetic modulation of gene expression is a major milestone for the understanding of diverse biological mechanisms and subsequently human pathologies. Although often evoked, little is ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.07.022
更新日期:2008-01-01 00:00:00
abstract::We extracted from L. biflexa patoc a fraction F, reacting in hemagglutination and ring tests with sera prepared against more than ten different serogroups. This fraction contains mainly a polysaccharide (65 per cent), the role of which was clearly demonstrated in the precipitation reaction with homologous antisera, th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80313-6
更新日期:1976-01-01 00:00:00