Abstract:
:The concept of a stem cell subpopulation as understood from normal epithelial tissue or bone marrow function has been extended to our understanding of cancer tissue and is now the target of treatment efforts specifically directed to this subpopulation. In glioblastoma, as well as in other cancers, increased expression of aldehyde dehydrogenase (ALDH) has been found localized within a minority sub-population of tumor cells which demonstrate stem cell properties. A separate body of research associated increased expression of heat-shock protein-90 (HSP90) with stem cell attributes. We present here results from our initial immunohistochemistry study of human glioblastoma biopsy tissue where both ALDH and HSP90 tended to be co-expressed in high amounts in the same minority of cells. Since 12% of all cells in the six biopsies studied were ALDH positive and 17% were HSP90 positive, by chance alone 2% would have been expected to be positive for both. In fact 7% of all cells simultaneously expressed both markers-a significant difference (p = 0.037). That two previously identified proteins associated with stem cell attributes tend to be co-expressed in the same individual glioblastoma cells might have clinical utility. Disulfiram, used to treat alcoholism for half-a century now, is a potent ALDH inhibitor and the old anti-viral drug ritonavir inhibits HSP90. These should be explored for the potential to retard aspects of glioblastoma stem cells' function subserved by ALDH and HSP90.
journal_name
Biochimiejournal_title
Biochimieauthors
Rappa F,Cappello F,Halatsch ME,Scheuerle A,Kast REdoi
10.1016/j.biochi.2012.11.007subject
Has Abstractpub_date
2013-04-01 00:00:00pages
782-6issue
4eissn
0300-9084issn
1638-6183pii
S0300-9084(12)00452-Xjournal_volume
95pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Inhibition of polypeptide chain elongation with the mRNA-complementary (antisense) oligonucleotide has been realized through a RNase H independent mechanism. Nuclease resistant complementary non-natural alpha-17-mer oligonucleotide did not inhibit cell-free protein biosynthesis of beta-globin in the wheat germ system ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(92)90089-w
更新日期:1992-05-01 00:00:00
abstract::Ligation of CD47 by its natural ligand thrombospondin (TSP), or cross-linking by CD47 antibodies, triggers caspase-independent cell death in normal and leukemic cells. This kind of cell death is characterised by the cytoplasmic events of apoptosis including externalisation of phosphatidylserines and mitochondria swell...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00129-9
更新日期:2003-08-01 00:00:00
abstract::In the recent years, we have provided evidence that Tumor Protein 53-Induced Nuclear Protein 1 (TP53INP1) is a key stress protein with antioxidant-associated tumor suppressive function. The TP53INP1 gene, which is highly conserved in mammals, is over-expressed during stress responses including inflammation. This gene ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.07.024
更新日期:2015-11-01 00:00:00
abstract::Our understanding of plant meiosis is rapidly increasing thanks to the model Arabidopsis thaliana. Here we present the results of a screening for meiotic mutants carried out with a library containing 30,719 T-DNA insertion lines. An average of one mutant per 1000 lines was recovered. Several phenotypic classes could b...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(01)01348-7
更新日期:2001-11-01 00:00:00
abstract::Concern about the malicious applications of botulinum neurotoxin has highlighted the need for a new generation of safe and highly potent antitoxins. In this study, we developed and evaluated the preclinical pharmacology and safety of a new F(ab')₂ antitoxin against botulinum neurotoxin serotype A (BoNT/A). As an alter...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.06.010
更新日期:2010-10-01 00:00:00
abstract::The ability of asialo-thyroglobulin to bind the thyroid RHL-1 subunit of the asialoglycoprotein receptor has been investigated. Ligand blot assays show that the recombinant carbohydrate recognition domain of the thyroid RHL-1 subunit specifically interacts with rat desialated thyroglobulin. Moreover, RT-PCR and Wester...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80100-x
更新日期:1999-05-01 00:00:00
abstract::A thrombin-like enzyme, named BjussuSP-I, isolated from Bothrops jararacussu snake venom, is an acidic single-chain glycoprotein with M(r)=61,000, pI approximately 3.8 and 6% sugar. BjussuSP-I shows high proteolytic activity upon synthetic substrates, such as S-2238 and S-2288. It also shows procoagulant and kallikrei...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.10.005
更新日期:2008-03-01 00:00:00
abstract::To elucidate the metabolic function of mRNA polyadenylation in Escherichia coli. we searched for a polyadenylate-binding protein as a potential mediator of the function of the poly(A) moiety. Using a nitrocellulose filter-binding assay and a Northwestern blot technique, a protein in the ribosomal supernatant fraction ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)82741-1
更新日期:1997-09-01 00:00:00
abstract::Genetic elements coding for proteins that present amino acid identity with the conserved motifs of retroviral reverse transcriptases constitute the retroid family. With the exception of reverse transcriptases encoded by mitochondrial plasmids of Neurospora, all reverse transcriptases have an absolute requirement for a...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(96)88114-4
更新日期:1995-01-01 00:00:00
abstract::Nicotinate phosphoribosyltransferase (NaPRT, EC 2.4.2.11) catalyzes the conversion of nicotinate (Na) to nicotinate mononucleotide, the first reaction of the Preiss-Handler pathway for the biosynthesis of NAD(+). Even though NaPRT activity has been described to be responsible for the ability of Na to increase NAD(+) l...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.06.033
更新日期:2012-02-01 00:00:00
abstract::We have modified elongation factor Tu (EF-Tu) from Escherichia coli via mutagenesis of its encoding tufA gene to study its function-structure relationships. The isolation of the N-terminal half molecule of EF-Tu (G domain) has facilitated the analysis of the basic EF-Tu activities, since the G domain binds the substra...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90147-s
更新日期:1991-07-01 00:00:00
abstract::Structural and motional features in the denatured state of a protein dictate the early folding events starting from that state and these features vary depending upon the nature of the denaturant used. Here, we have attempted to decipher the early events in the folding of Dynein Light Chain protein (DLC8), starting fro...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.10.013
更新日期:2012-01-01 00:00:00
abstract::Long non-coding RNAs constitute the most abundant part of the transcribed mammalian genome. lncRNAs affect all essential processes in the living cell including transcription, splicing, translation, replication, shaping of chromatin and post translational modification of proteins. Alterations in lncRNA expression have ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2016.06.007
更新日期:2016-12-01 00:00:00
abstract::We describe PGtrans, a new and freely available protein sequence databank (2625 sequences, 554198 amino-acids). This data bank is routinely produced by automatic computer translation of the nucleotide sequence library GenBank. The information needed for the translation process (transcriptional orientation, location of...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80261-3
更新日期:1985-05-01 00:00:00
abstract::Peptides with hormonal or neuronal activity are derived by enzymatic processing from pro-hormones, which by themselves are biologically inert. Processing and other enzymatic conversions may occur step-wise, leading to the formation of a cascade of biologically active (or inactive) peptides. The neurokin in substance P...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90159-9
更新日期:1988-01-01 00:00:00
abstract::Phosphorus metabolites and intracellular pH have been examined in the slime mold Dictyostelium discoideum by non-destructive 31P-NMR measurements. In a spectrum from a suspension of aerobic amoebae, the major peaks are inorganic phosphate, nucleotide di- and triphosphates. In the corresponding perchloric acid extract,...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(86)80077-3
更新日期:1986-12-01 00:00:00
abstract::Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)88887-2
更新日期:1998-11-01 00:00:00
abstract::This review discusses the regulation of a key controlling step in the initiation of protein synthesis, the binding of mRNA to ribosomes. Particular focus is given to the phosphorylation of the cap-binding factor, eIF-4E, and the role of this factor in the regulation of cell growth. ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(94)90185-6
更新日期:1994-01-01 00:00:00
abstract::We have analysed the complete sequence of the Escherichia coli K12 isolate MG1655 genome for chromatin-associated protein binding sites, and compared the predicted location of predicted sites with experimental expression data from 'DNA chip' experiments. Of the dozen proteins associated with chromatin in E. coli, only...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)01225-6
更新日期:2001-02-01 00:00:00
abstract::Recently we have found that cytokine IL-2 and innate immunity protein Tag7 activate cytotoxic lymphocytes that kill HLA-negative tumor cells, inducing both apoptosis and necroptosis. Here we decrypt the processes, taking part in necroptosis execution after FasL-Fas interaction. Necroptosis begins with RIPK1 activation...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.07.003
更新日期:2018-09-01 00:00:00
abstract::Glyoxalate is an effector of oxidative phosphorylation in isolated mitochondria : it slows down State 3 but does not affect State 4 respiration. This report presents the findings of our study on the mechanism of action of glyoxalate ; these findings are listed below. The inhibition of Stage 3 respiration by glyoxalate...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(75)80145-3
更新日期:1975-01-01 00:00:00
abstract::The specific binding of the amyloid protein precursor (APP) to glycosaminoglycans (GAG) suggests that APP is a cell adhesion molecule (CAM) and/or substrate adhesion molecule (SAM). In order to characterize this activity of APP in the brain at the molecular level, we have purified and characterized the major APP speci...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(94)90163-5
更新日期:1994-01-01 00:00:00
abstract::A novel approach to genetic sequence analysis is presented. This approach, based on compression of algorithms, has been launched simultaneously by Grumbach and Tahi, Milosavljevic and Rivals. To reduce the description of an object, a compression algorithm replaces some regularities in the description by special codes....
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)84763-8
更新日期:1996-01-01 00:00:00
abstract::The rat and rabbit lung isoform pattern before (fetal) and after (adult) lung maturation has been analyzed by isoelectric focusing. This pattern has been compared to that of brain tubulin at the same developmental stages. No changes in the pattern of fetal and adult lung tubulin were found. However an increase in brai...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80301-1
更新日期:1985-09-01 00:00:00
abstract::Gallium, a group IIIa metal salt, has been demonstrated to be an effective immunosuppressive agent. Gallium has also been shown to inhibit the production of inflammatory cytokines, such as IL-1beta, produced by macrophage-like cells in vitro. To further characterize the effects of gallium on the inflammatory process, ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)00384-9
更新日期:2000-02-01 00:00:00
abstract::Bacillus subtilis strain SO113 secretes a pectate lyase which is produced during the exponential death phase of growth, just before sporulation. This extracellular pectate lyase, which produces unsaturated products from polygalacturonate, was purified 35-fold from the culture supernatant of Bacillus subtilis by a CM S...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90053-j
更新日期:1990-09-01 00:00:00
abstract::Epigenetic mechanisms are likely to play a role in many complex diseases, the extent of which we only beginning to understand. COPD and asthma are two respiratory diseases subject to strong environmental influences depending on underlying genetic susceptibility. Epigenetic mechanisms such as DNA methylation, histone m...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.07.017
更新日期:2012-11-01 00:00:00
abstract::gamma-Aminobutyraldehyde dehydrogenase from Escherichia coli K-12 has been purified and characterized from cell mutants able to grow in putrescine as the sole carbon and nitrogen source. The enzyme has an Mr of 195,000 +/- 10,000 in its dimeric form with an Mr of 95,000 +/- 1,000 for each subunit, a pH optimum at 5.4 ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(87)90142-8
更新日期:1987-11-01 00:00:00
abstract::Cell sterol supply is subjected to tight negative feedback regulation through the SREBP pathway. Upon cholesterol depletion, SREBP transcription factors become activated by cleavage of a membrane bound precursor form, which stimulates the expression of the genes encoding proteins of the cholesterol synthesis pathway. ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2006.09.015
更新日期:2007-02-01 00:00:00
abstract::The lignan 8,8'-bis-(methylenedioxy)cinnamic acid (BMDCA) is a powerful competitive inhibitor (K1 = 2.0 microM) of the lignin peroxidase (LiP) from Phanerochaete chrysosporium and of the extracellular peroxidase of Phlebia radiata (I0.5 = 10 microM). BMDCA derivatives with the same double bond system also inhibited th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)88186-7
更新日期:1995-01-01 00:00:00