Abstract:
:Gallium, a group IIIa metal salt, has been demonstrated to be an effective immunosuppressive agent. Gallium has also been shown to inhibit the production of inflammatory cytokines, such as IL-1beta, produced by macrophage-like cells in vitro. To further characterize the effects of gallium on the inflammatory process, we examined the effects of gallium nitrate on matrix metalloproteinase (MMP) activity utilizing the rabbit synoviocyte cell line HIG-82. HIG-82 cells were incubated with IL-1beta and TPA, with and without increasing concentrations of gallium nitrate. Conditioned medium was collected and assayed for MMP activity using a synthetic substrate and substrate gel zymography. IL-1beta and TPA alone induced MMP activity in HIG-82 cells. A dose-dependent inhibition of IL-1beta and TPA stimulated MMP activity by gallium nitrate at increasing concentrations was observed. This study demonstrates that gallium nitrate can inhibit the activity of MMPs and may be useful as a modulator of inflammation in arthritis.
journal_name
Biochimiejournal_title
Biochimieauthors
Panagakos FS,Kumar E,Venescar C,Guidon Pdoi
10.1016/s0300-9084(00)00384-9keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
147-51issue
2eissn
0300-9084issn
1638-6183pii
S0300-9084(00)00384-9journal_volume
82pub_type
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