Polymers containing isonitrile functional groups as supports for the covalent fixation of biologically active molecules.

abstract：:The emergence of drug-resistant Mycobacterium tuberculosis (Mtb) stains has escalated the need for developing more efficient drugs and therapeutic strategies against tuberculosis. Here we functionally annotate a secretory mycobacterial asparaginase Rv1538c (MtA) and describe its biochemical properties. MtA primarily e...

journal_title：Biochimie

authors： Kataria A,Patel AK,Kundu B

更新日期：2021-01-05 00:00:00

abstract：:pH-Dependence of hydrolytic activity of trypsin has been studied in cationic reverse micellar system of cetyltrimethylammonium bromide (CTAB) in (50% v/v) chloroform/isooctane using a positively charged substrate N(alpha)-benzoyl-L-arginine ethyl ester (BAEE). The pH of the medium was varied from 4.0 to 8.5 with addit...

journal_title：Biochimie

authors： Fadnavis NW,Babu RL,Deshpande A

更新日期：1998-12-01 00:00:00

abstract：:The cholesterol lowering drug lovastatin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, blocks DNA synthesis and proliferation of thyrotropin (TSH) primed FRTL-5 rat thyroid cells. The blockade can be completely prevented and/or reversed by mevalonate and largely prevented and/o...

journal_title：Biochimie

authors： Bifulco M,Laezza C,Aloj SM

更新日期：1999-04-01 00:00:00

abstract：:Some physico-chemical properties of R140G and K141Q mutants of human small heat shock protein HspB1 associated with hereditary peripheral neuropathy were analyzed. Mutation K141Q did not affect intrinsic Trp fluorescence and interaction with hydrophobic probe bis-ANS, whereas mutation R140G decreased both intrinsic fl...

journal_title：Biochimie

authors： Nefedova VV,Datskevich PN,Sudnitsyna MV,Strelkov SV,Gusev NB

更新日期：2013-08-01 00:00:00

abstract：:The proteasome activators known as 11S REG or PA28 were discovered about 10 years ago. They are homo- or heteroheptameric rings that bind to the ends of 20S proteasomes and activate cleavage of peptides but not folded proteins. In this article, we focus on structural features of three homologous REG subunits (termed a...

journal_title：Biochimie

authors： Li J,Rechsteiner M

更新日期：2001-03-01 00:00:00

abstract：:Homocysteine (HCY) is a degradation product of the methionine pathway. The B vitamins, in particular vitamin B12 and folate, are the primary nutritional determinant of HCY levels and therefore their deficiencies result in hyperhomocysteinaemia (HHCY). Prevalence of hyperhomocysteinemia (HHCY) and related dietary defic...

journal_title：Biochimie

authors： Feigerlova E,Demarquet L,Guéant JL

更新日期：2016-07-01 00:00:00

abstract：:We have modified elongation factor Tu (EF-Tu) from Escherichia coli via mutagenesis of its encoding tufA gene to study its function-structure relationships. The isolation of the N-terminal half molecule of EF-Tu (G domain) has facilitated the analysis of the basic EF-Tu activities, since the G domain binds the substra...

journal_title：Biochimie

authors： Anborgh PH,Cool RH,Gümüsel F,Harmark K,Jacquet E,Weijland A,Mistou MY,Parmeggiani A

更新日期：1991-07-01 00:00:00

abstract：:The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central ner...

journal_title：Biochimie

authors： Sarbu M,Raab S,Henderson L,Fabris D,Vukelić Ž,Clemmer DE,Zamfir AD

更新日期：2020-03-01 00:00:00

abstract：:Peroxisomal ABC transporters encoded by the ABCD genes are thought to participate in the import of specific fatty acids in the peroxisomal matrix. ABCD1 deficiency is associated with X-linked adrenoleukodystrophy (X-ALD), the most frequent peroxisomal disorder which is characterized by the accumulation of saturated ve...

journal_title：Biochimie

authors： Leclercq S,Skrzypski J,Courvoisier A,Gondcaille C,Bonnetain F,André A,Chardigny JM,Bellenger S,Bellenger J,Narce M,Savary S

更新日期：2008-10-01 00:00:00

abstract：:PA1b (Pea Albumin 1b) is a peptide toxin lethal for certain insects. This paper shows that the cultured insect cells Sf9 are sensitive to the toxin and display a high-affinity binding site for PA1b. Mammalian cells are not sensitive and no binding activity was detected. Signs of apoptosis of the Sf9 cells were observe...

journal_title：Biochimie

authors： Rahioui I,Laugier C,Balmand S,Da Silva P,Rahbe Y,Gressent F

更新日期：2007-12-01 00:00:00

abstract：BACKGROUND:Fatty acids (FAs), and especially monounsaturated FAs (MUFAs) stimulate GLP-1 release. However, lipotoxicity is indicated in GLP-1 secreting cells following long-term exposure to elevated levels of saturated FAs (SFAs) in vivo and in vitro, where in vitro studies indicate that cosupplementation with MUFAs co...

journal_title：Biochimie

authors： Ntika S,Thombare K,Aryapoor M,Kristinsson H,Bergsten P,Krizhanovskii C

更新日期：2019-04-01 00:00:00

abstract：:Human mitochondrial diseases, defined as the diseases due to a mitochondrial oxidative phosphorylation defect, represent a large group of very diverse diseases with respect to phenotype and genetic causes. They present with many unsolved issues, the comprehensive analysis of which is beyond the scope of this review. W...

journal_title：Biochimie

authors： Lombès A,Auré K,Bellanné-Chantelot C,Gilleron M,Jardel C

更新日期：2014-05-01 00:00:00

abstract：:Identification of proteoglycans in two human malignant mesothelioma cell lines, one with epithelial differentiation and the other with fibroblast-like phenotype, and the effects of epidermal (EGF), insulin-like (IGF-I) and platelet-derived (PDGF-BB) growth factors on the synthesis of hyaluronan (HA) and proteoglycans ...

journal_title：Biochimie

authors： Syrokou A,Tzanakakis GN,Hjerpe A,Karamanos NK

更新日期：1999-07-01 00:00:00

abstract：:Leishmania parasites cause two million new cases of leishmaniasis each year with several hundreds of millions of people at risk. Due to the paucity and shortcomings of available drugs, we have undertaken the crystal structure determination of a key enzyme from Leishmania major in hopes of creating a platform for the r...

journal_title：Biochimie

authors： Larson ET,Kim JE,Zucker FH,Kelley A,Mueller N,Napuli AJ,Verlinde CL,Fan E,Buckner FS,Van Voorhis WC,Merritt EA,Hol WG

更新日期：2011-03-01 00:00:00

abstract：:Kinetic studies indicate that glucose-6-phosphatase is a multifunctional enzyme. a) Phosphohydrolase activities. The mannose-6-phosphatase activity is low (Km = 8 mM, VM = 90 nmoles. min-1mg-1). The enzyme shows a strong affinity for glucose-6-phosphate (Km = 2.5 mM, VM = 220 nmoles.min-1mg-1). beta-glycerophosphate (...

journal_title：Biochimie

authors： Benedetto JP,Martel MB,Got R

更新日期：1979-01-01 00:00:00

abstract：:Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neu1 physiologic...

journal_title：Biochimie

authors： Ryuzono S,Takase R,Kamada Y,Ikenaga T,Chigwechokha PK,Komatsu M,Shiozaki K

更新日期：2017-04-01 00:00:00

abstract：:We have previously shown that withdrawal of folic acid led to metabolic reprogramming and a less aggressive phenotype in a mouse cell model of triple-negative breast cancer (TNBC). Herein, we evaluate the effects of folic acid withdrawal on transcriptomic profiles in these cells. Murine cell lines were originally deri...

journal_title：Biochimie

authors： Kok DE,O'Flanagan CH,Coleman MF,Ashkavand Z,Hursting SD,Krupenko SA

更新日期：2020-06-01 00:00:00

abstract：:We screened a cDNA library of the primitive eukaryote Dictyostelium discoideum constructed in the expression vector lambda gt11 with a specific antiserum directed against S-adenosyl-L-homocysteine hydrolase (AdoHcy hydrolase) and isolated cDNA clones coding for fusion proteins with beta-galactosidase. The identity of ...

journal_title：Biochimie

authors： Guitton MC,Part D,Veron M

更新日期：1988-06-01 00:00:00

abstract：:Lipids are unevenly distributed within eukaryotic cells, allowing various processes to work in an optimized membrane environment. Rather than following vesicular trafficking pathways, certain lipids are transported by lipid transfer proteins (LTPs), some at sites of close apposition of two organelles called membrane c...

journal_title：Biochimie

authors： Moser von Filseck J,Drin G

更新日期：2016-11-01 00:00:00

abstract：:The functions of residues 57-RY-58, G60, L77, 80-GSGR-83, I101, T104, 134-GY-135, N155, V157 and 160-LV-161 in human arsenic (III) methyltransferase (hAS3MT) 5 Å around S-adenosylmethionine (SAM) have not been studied. Herein, sixteen mutants were designed by substituting these residues with Ala. Mutants G60A, G80A, I...

journal_title：Biochimie

authors： Li X,Geng Z,Chang J,Song X,Wang Z

更新日期：2014-12-01 00:00:00

abstract：:Mitochondrial function requires genes encoded in both mitochondrial and nuclear genomes. Tfam, the activator of mammalian mitochondrial transcription, is encoded in the nucleus and its expression has been shown in in vitro studies to be controlled by nuclear respiratory factors NRF-1 and NRF-2. In order to understand ...

journal_title：Biochimie

authors： Escrivá H,Rodríguez-Peña A,Vallejo CG

更新日期：1999-10-01 00:00:00

abstract：:Virtually all brain circuits are physically embedded in a three-dimensional matrix of fibers that release 5-hydroxytryptamine (5-HT, serotonin). The density of this matrix varies across brain regions and cortical laminae, and it is altered in some mental disorders, including Major Depressive Disorder and Autism Spectr...

journal_title：Biochimie

authors： Janušonis S,Detering N

更新日期：2019-06-01 00:00:00

abstract：:Poly(ADP-ribosyl)ation is a posttranslational protein modification significant for genomic stability and cell survival in response to DNA damage. Poly(ADP-ribosyl)ation is catalyzed by poly(ADP-ribose)polymerases (PARPs). Among the 17 members of the PARP family, PARP-1 and PARP-2 are described as enzymes whose catalyt...

journal_title：Biochimie

authors： Kutuzov MM,Khodyreva SN,Amé JC,Ilina ES,Sukhanova MV,Schreiber V,Lavrik OI

更新日期：2013-06-01 00:00:00

abstract：:GABARAPL1 belongs to the small family of GABARAP proteins (including GABARAP, GABARAPL1 and GABARAPL2/GATE-16), one of the two subfamilies of the yeast Atg8 orthologue. GABARAPL1 is involved in the intracellular transport of receptors, via an interaction with tubulin and GABA(A) or kappa opioid receptors, and also par...

journal_title：Biochimie

authors： Seguin-Py S,Lucchi G,Croizier S,Chakrama FZ,Despouy G,Le Grand JN,Ducoroy P,Boireau W,Boyer-Guittaut M,Jouvenot M,Fraichard A,Delage-Mourroux R

更新日期：2012-03-01 00:00:00

abstract：:Versican and decorin, two proteoglycans (PGs) with contradictory roles in the pathophysiology of cancer, comprise important stromal components in many tumor types and play a crucial role in the progression of cancer. In this study, we provide direct evidence for a significant and stage-related accumulation of versican...

journal_title：Biochimie

authors： Skandalis SS,Theocharis AD,Papageorgakopoulou N,Vynios DH,Theocharis DA

更新日期：2006-09-01 00:00:00

abstract：:The translation factor EF-P is required for efficient prokaryotic peptide bond synthesis on 70S ribosomes from fMet-tRNAfMet. This protein has been purified from Escherichia coli cells and the gene, efp, encoding it has been cloned and sequenced. We have isolated recombinant clones which overexpress a protein that co-...

journal_title：Biochimie

authors： Aoki H,Adams SL,Turner MA,Ganoza MC

更新日期：1997-01-01 00:00:00

abstract：:In vitro cycling rates of E. coli ribosomes and of elongation factors EF-Tu and EF-G have been obtained and these are compatible with translation rates in vivo. We show that the rate of translocation is faster than 50 s-1 and therefore that the EF-G function is not a rate limiting step in protein synthesis. The in viv...

journal_title：Biochimie

authors： Bilgin N,Kirsebom LA,Ehrenberg M,Kurland CG

更新日期：1988-05-01 00:00:00

abstract：:We have proposed an allosteric ATP inhibition mechanism of Pfk-2 determining the structure of different forms of the enzyme together with a kinetic enzyme analysis. Here we complement the mechanism by using hybrid oligomers of the homodimeric enzyme to get insights about the allosteric communication pathways between t...

journal_title：Biochimie

authors： Villalobos P,Soto F,Baez M,Babul J

更新日期：2016-09-01 00:00:00

abstract：:The solubilizing powers of taurocholate, taurochenodeoxycholate and tauroursodeoxycholate for monoolein and cholesterol, and the size of the bile salt-monoolein-cholesterol micelles have been determined. For the three bile salt species, the micellar size depends on the saturation with monoolein. As a result, for a giv...

journal_title：Biochimie

authors： Montet JC,Lindheimer M,Reynier MO,Crotte C,Bontemps R,Gerolami A

更新日期：1982-04-01 00:00:00

abstract：:Using a new extended set of 13 amber suppressors in E coli, systematic amino-acid replacements were performed at positions 104(E) and 238(G) of TEM-1 beta-lactamase from PUC19. The enzyme is tolerant to most substitutions tested at position 104. Missense revertants E104K, E104S or E104Y exhibited only minor changes in...

journal_title：Biochimie

authors： Lenfant F,Labia R,Masson JM

更新日期：1990-06-01 00:00:00