Running up that hill: How to create cellular lipid gradients by lipid counter-flows.

abstract：:A major incentive in inhibitor research is that control of limited proteolysis constitutes a valuable pharmacological tool. Protease inhibitors have proved to be successful in influencing pathogenesis in many experimental models but a breakthrough to use in human therapy has mainly been restricted to aprotinin and ang...

journal_title：Biochimie

authors： Scharpe S,De Meester I,Hendriks D,Vanhoof G,van Sande M,Vriend G

更新日期：1991-01-01 00:00:00

abstract：:Kissing loop interactions (KLIs) are a common motif that is critical in retroviral dimerization, viroid replication, mRNA, and riboswitches. In addition, KLIs are currently used in a variety of biotechnology applications, such as in aptamer sensors, RNA scaffolds and to stabilize vaccines for therapeutics. Here we des...

journal_title：Biochimie

authors： Carr CE,Marky LA

更新日期：2019-02-01 00:00:00

abstract：:Genetic elements coding for proteins that present amino acid identity with the conserved motifs of retroviral reverse transcriptases constitute the retroid family. With the exception of reverse transcriptases encoded by mitochondrial plasmids of Neurospora, all reverse transcriptases have an absolute requirement for a...

journal_title：Biochimie

authors： Marquet R,Isel C,Ehresmann C,Ehresmann B

更新日期：1995-01-01 00:00:00

abstract：:The molecular basis for the essential role of cholesterol in mammalian (and other cholesterol-requiring) cells has long been the object of intense interest. Cholesterol has been found to modulate the function of membrane proteins critical to cellular function. Current literature supports two mechanisms for this modula...

journal_title：Biochimie

authors： Yeagle PL

更新日期：1991-10-01 00:00:00

abstract：:The nuclear metabolism of poly(ADP-ribose) is mainly regulated by poly(ADP-ribose) polymerase-1 (PARP-1) and by poly(ADP-ribose) glycohydrolase (PARG). A PARP-like enzyme, V-PARP, and a PARG isoform are present in the extra-nuclear compartment of mammalian cells, even if poly(ADP-ribose) has never been detected therei...

journal_title：Biochimie

authors： Rossi L,Denegri M,Torti M,Poirier GG,Ivana Scovassi A

更新日期：2002-12-01 00:00:00

abstract：:Lysophosphatidic acid (LPA), a naturally occurring bioactive phospholipid, mediates a multitude of (patho)physiological events including activation of mitogen-activated protein kinases (MAPKs). As LPA may induce cellular reponses in human osteosarcoma, the present study aimed at investigating expression of various LPA...

journal_title：Biochimie

authors： Windischhofer W,Huber E,Rossmann C,Semlitsch M,Kitz K,Rauh A,Devaney T,Leis HJ,Malle E

更新日期：2012-09-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris is often used as an organism for the heterologous expression of proteins and has been used already for production of a number of glycosyltransferases involved in the biosynthesis of N- and O-linked oligosaccharides. In our recent studies, we have examined the expression in P. p...

journal_title：Biochimie

authors： Bencúrová M,Rendić D,Fabini G,Kopecky EM,Altmann F,Wilson IB

更新日期：2003-03-01 00:00:00

abstract：:When liver cells from either normal or hereditarily diabetic rats are exposed to (13)C-enriched D-fructose (10 mM) and unlabelled D-glucose (also 10 mM) in the presence of D(2)O, the output of (13)C-enriched D-glucose generated from D-[1-(13)C]fructose is significantly lower than that from D-[2-(13)C]fructose. This co...

journal_title：Biochimie

authors： Malaisse WJ,Willem R

更新日期：2004-02-01 00:00:00

abstract：:As part of a project aimed at obtaining selective inhibitors and drug-like compounds targeting tRNA synthetases from trypanosomatids, we have elucidated the crystal structure of human cytosolic histidyl-tRNA synthetase (Hs-cHisRS) in complex with histidine in order to be able to compare human and parasite enzymes. The...

journal_title：Biochimie

authors： Koh CY,Wetzel AB,de van der Schueren WJ,Hol WG

更新日期：2014-11-01 00:00:00

abstract：:The synthesis of holocytochromes in plastids is a catalyzed process. Several proteins, including plastid CcsA, Ccs1, possibly CcdA and a thioredoxin, plus at least two additional Ccs factors, are required in sub-stoichiometric amounts for the conversion of apocytochromes f and c(6) to their respective holoforms. CcsA,...

journal_title：Biochimie

authors： Nakamoto SS,Hamel P,Merchant S

更新日期：2000-06-01 00:00:00

abstract：:In order to identify conserved potential secondary structures within translational start sites, mRNA sequences derived from different species were studied with programs able to depict such features. The potential secondary structure of 71 bases around the initiator AUG or AUGs in the coding sequences of 290 eukaryotic...

journal_title：Biochimie

authors： Ganoza MC,Louis BG

更新日期：1994-01-01 00:00:00

abstract：:Epidemiological studies have demonstrated that the diabetes mellitus is a serious health burden for both governments and healthcare providers. The present study was hypothesized to evaluate the antihyperglycemic potential of fraxetin by determining the activities of key enzymes of carbohydrate metabolism in streptozot...

journal_title：Biochimie

authors： Murali R,Srinivasan S,Ashokkumar N

更新日期：2013-10-01 00:00:00

abstract：:A 3-D model of the tyrosylable tRNA-like domain of the genomic brome mosaic virus RNAs was built by computer modelling based on solution probing of the molecule with different chemical and enzymatic reagents. This model encompasses four major structural domains, including two peculiar substructures oriented perpendicu...

journal_title：Biochimie

authors： Felden B,Florentz C,Westhof E,Giegé R

更新日期：1993-01-01 00:00:00

abstract：:Sm and Sm-like (Lsm) proteins are considered as an evolutionary conserved family involved in RNA metabolism in organisms from bacteria and archaea to human. Currently, the function of Sm-like archaeal proteins (SmAP) is not well understood. Here, we report the crystal structures of SmAP proteins from Sulfolobus acidoc...

journal_title：Biochimie

authors： Lekontseva N,Mikhailina A,Fando M,Kravchenko O,Balobanov V,Tishchenko S,Nikulin A

更新日期：2020-08-01 00:00:00

abstract：:The Gag-encoded nucleocapsid protein NCp7 (72 amino acids) from HIV-1, the regulatory protein, Vpr (96 amino acids) and numerous derivatives have been synthesized by solid phase method and their structures determined by 2D NMR. In NCp7, the two highly folded zinc fingers of the Cx2Cx4Hx4C type are in close spacial pro...

journal_title：Biochimie

authors： Roques BP,Morellet N,de Rocquigny H,Déméné H,Schueler W,Jullian N

更新日期：1997-11-01 00:00:00

abstract：:Disintegrins are small non-enzymatic proteins common in the venoms of many viperid snakes. These proteins have received significant attention due to their ability to inhibit platelet aggregation and cell adhesion, making them model compounds in drug development and design investigations. The present study used a combi...

journal_title：Biochimie

authors： Saviola AJ,Modahl CM,Mackessy SP

更新日期：2015-09-01 00:00:00

abstract：:Mouse brain myelin consists of a continuum of particles of different densities, as shown by sucrose density gradient centrifugation. In normal animals most of the material (65 per cent) is concentrated between 0.6 and 0.7 M sucrose (the maximum being found at 0.66 M sucrose, corresponding to 23 per cent). The density ...

journal_title：Biochimie

authors： Bourre JM,Pollet S,Daudu O,Le Saux F,Baumann N

更新日期：1977-01-01 00:00:00

abstract：:There are five subtypes of muscarinic acetylcholine receptors (M(1) to M(5)) which control a large number of physiological processes, such as the function of heart and smooth muscles, glandular secretion, release of neurotransmitters, gene expression and cognitive functions as learning and memory. A selective ligand i...

journal_title：Biochimie

authors： Karlsson E,Jolkkonen M,Mulugeta E,Onali P,Adem A

更新日期：2000-09-01 00:00:00

abstract：:Aptamers are short single-stranded nucleic acid molecules that are selected in vitro from a large random sequence library based on their high and specific affinity to a target molecule by a process known as SELEX. Cell-SELEX that employs whole living cells overexpressing the defined cell surface proteins (for selectio...

journal_title：Biochimie

authors： Takahashi M,Sakota E,Nakamura Y

更新日期：2016-12-01 00:00:00

abstract：:Intracellular cytoplasmic peptidoglycan (PG) intermediate levels were determined in Staphylococcus aureus during log-phase growth in enriched media. Levels of UDP-linked intermediates were quantitatively determined using ion pairing LC-MS/MS in negative mode, and amine intermediates were quantitatively determined ster...

journal_title：Biochimie

authors： Vemula H,Ayon NJ,Gutheil WG

更新日期：2016-02-01 00:00:00

abstract：:An alkaline aminopeptidase was isolated from the culture medium of Penicillium roqueforti. The enzyme was purified by ammonium sulfate precipitation, filtration on Bio-Gel P-100, chromatography on D.E.A.E.-cellulose and hydroxylapatite, filtration on Bio-Gel P-150 and electrofusing. The purified preparation was homoge...

journal_title：Biochimie

authors： Gripon JC

更新日期：1977-01-01 00:00:00

abstract：:Excessive tissue iron levels are associated with the increase of oxidative/nitrative stress which contributes to tissue damage that may elevate the risk of diabetes. Therefore, we investigated the effects of iron on diabetes-associated liver injury and whether iron-related tyrosine nitration participated in this proce...

journal_title：Biochimie

authors： Li X,Li H,Lu N,Feng Y,Huang Y,Gao Z

更新日期：2012-12-01 00:00:00

abstract：:The issue of whether histone H1 possesses specificity of binding to certain nucleotide sequences in DNA is of fundamental importance to the suggested role of the linker histone in the regulation of gene transcription. The purpose of the present study was to reinvestigate the specificity of binding of histone H1 to the...

journal_title：Biochimie

authors： Yaneva JN,Zlatanova JS

更新日期：1993-01-01 00:00:00

abstract：:Diacylglycerol kinase (DGK) phosphorylates diacylglycerol to produce phosphatidic acid (PA). The η isozyme of DGK is abundantly expressed in C2C12 myoblasts. However, the role of DGKη in skeletal muscle cells remains unknown. In the present study, we showed that DGKη was downregulated at an early stage of myogenic dif...

journal_title：Biochimie

authors： Sakai H,Murakami C,Usuki T,Lu Q,Matsumoto KI,Urano T,Sakane F

更新日期：2020-10-01 00:00:00

abstract：:The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...

journal_title：Biochimie

authors： Masson O,Bach AS,Derocq D,Prébois C,Laurent-Matha V,Pattingre S,Liaudet-Coopman E

更新日期：2010-11-01 00:00:00

abstract：:Reuber H 35 hepatoma cells were synchronized by transfer in a serum free medium. Growth was re-initiated by addition of serum. Under these conditions DNA synthesis exhibited a maximum after 24 hours. Chromatin non-histone proteins prepared from cells at various phases of the cell cycle were incubated with [gamma-32P] ...

journal_title：Biochimie

authors： Defer N,Tichonicky L,Kruh J,Zoutewelle G,Van Wijk R

更新日期：1979-01-01 00:00:00

abstract：:Information on the subcellular localization of bacterial proteins is essential for protein function prediction, genome annotation and drug design. Here we proposed a novel approach to predict the subcellular localization of bacterial proteins by fusing features from position-specific score matrix (PSSM), Gene Ontology...

journal_title：Biochimie

authors： Li L,Yu S,Xiao W,Li Y,Li M,Huang L,Zheng X,Zhou S,Yang H

更新日期：2014-09-01 00:00:00

abstract：:Porcine pancreatic extracts (PPE), also named pancreatin, are commonly used as a global source of pancreatic enzymes for enzyme replacement therapy in patients with exocrine pancreatic insufficiency. They are considered as a good substitute of human pancreatic enzymes and they have become a material of choice for in v...

journal_title：Biochimie

authors： Salhi A,Amara S,Mansuelle P,Puppo R,Lebrun R,Gontero B,Aloulou A,Carrière F

更新日期：2020-02-01 00:00:00

abstract：:A previous study has shown the effect of individual mineral surfaces on the activity of sweet almond beta-D-glucosidase. We now consider more complex situations likely to occur in soil, such as adsorption onto mixtures of different mineral surfaces, and the effect on enzyme activity of mineral surfaces with organic co...

journal_title：Biochimie

authors： Quiquampoix H

更新日期：1987-06-01 00:00:00

abstract：:We have modified elongation factor Tu (EF-Tu) from Escherichia coli via mutagenesis of its encoding tufA gene to study its function-structure relationships. The isolation of the N-terminal half molecule of EF-Tu (G domain) has facilitated the analysis of the basic EF-Tu activities, since the G domain binds the substra...

journal_title：Biochimie

authors： Anborgh PH,Cool RH,Gümüsel F,Harmark K,Jacquet E,Weijland A,Mistou MY,Parmeggiani A

更新日期：1991-07-01 00:00:00