Abstract:
:The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesicles (lysosomes and endosomes). In cancer cells, overexpressed cath-D accumulates in cells, where it may affect their degradative capacities, and the pro-enzyme is hyper-secreted in the tumor micro-environment. In addition, during apoptosis, lysosomal cath-D is released into the cytosol, where it may interact with and/or cleave pro-apoptotic, anti-apoptotic, or nuclear proteins. Several studies have shown that cath-D affects various different steps in tumor progression and metastasis. Cath-D stimulates cancer cell growth in an autocrine manner, and also cath-D plays a crucial paracrine role in the tumor micro-environment by stimulating fibroblast outgrowth and tumor angiogenesis. A mutant D231N-cath-D, which is devoid of catalytic activity, remained mitogenic, indicating an additional action of cath-D by protein-protein interaction. Targeting cath-D in cancer may require the use of inhibitors of its catalytic activity, but also the development of new tools to inhibit its protein binding functions. Thus, elucidation of the mechanism of action of cath-D is crucial if an appropriate strategy is to be developed to target this protease in cancer. The discovery of new physiological substrates of cath-D using proteomic approaches can be expected to generate new critical targets. The aim of this review is to describe the roles of the cath-D protease in cancer progression and metastasis, as well as its function in apoptosis, and to discuss how it can be targeted in cancer by inhibiting its proteolytic activity and/or its binding protein activity.
journal_name
Biochimiejournal_title
Biochimieauthors
Masson O,Bach AS,Derocq D,Prébois C,Laurent-Matha V,Pattingre S,Liaudet-Coopman Edoi
10.1016/j.biochi.2010.05.009subject
Has Abstractpub_date
2010-11-01 00:00:00pages
1635-43issue
11eissn
0300-9084issn
1638-6183pii
S0300-9084(10)00203-8journal_volume
92pub_type
杂志文章,评审相关文献
BIOCHIMIE文献大全abstract::With the increase of multidrug resistance, novel anti-leukemia agents with diverse mechanisms of action are required to address this challenge. NK-18, the core region of mammalian derived protein NK-lysin, effectively inhibited the viability of both multidrug resistant and sensitive leukemia cell lines. Meanwhile, thi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.02.001
更新日期:2018-04-01 00:00:00
abstract::In man the brain represents about 2% of the body weight, but contains 25% of the body's cholesterol. Cholesterol itself does not cross the blood-brain barrier and is synthesised in situ. Excess cholesterol from brain is exported in the form of oxysterols, or metabolised to steroids, which in contrast to cholesterol ca...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2006.10.008
更新日期:2007-02-01 00:00:00
abstract::After exhaustive pronase digestion, purification by gel filtration and affinity chromatography on concanavalin A, three glycopeptide fractions were obtained from rat hemopexin. Two fractions (I and II) were concanavalin A non-reactive and one (III) was concanavalin A reactive. On the basis of carbohydrate composition,...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(83)80083-2
更新日期:1983-03-01 00:00:00
abstract::Pentatricopeptide repeat (PPR) proteins are a large family of modular RNA-binding proteins which mediate several aspects of gene expression primarily in organelles but also in the nucleus. These proteins facilitate processing, splicing, editing, stability and translation of RNAs. While major advances in PPR research h...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.04.004
更新日期:2015-06-01 00:00:00
abstract::A 3-D model of the tyrosylable tRNA-like domain of the genomic brome mosaic virus RNAs was built by computer modelling based on solution probing of the molecule with different chemical and enzymatic reagents. This model encompasses four major structural domains, including two peculiar substructures oriented perpendicu...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(93)90014-j
更新日期:1993-01-01 00:00:00
abstract::Lipids are unevenly distributed within eukaryotic cells, allowing various processes to work in an optimized membrane environment. Rather than following vesicular trafficking pathways, certain lipids are transported by lipid transfer proteins (LTPs), some at sites of close apposition of two organelles called membrane c...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2016.08.001
更新日期:2016-11-01 00:00:00
abstract::Murine L5178Y cell ribosomes were dissociated into subunits either with potassium chloride in the presence of puromycin or with the chelating agent EDTA. The proteins of ribosomal subunits obtained by these different methods were compared by means of bidimensional polyacrylamide gel electrophoresis. KCl-derived 60S an...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80225-2
更新日期:1979-01-01 00:00:00
abstract::A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.02.018
更新日期:2008-06-01 00:00:00
abstract::Excitation-contraction coupling describes the series of events that beginning with propagated action potential on the muscle fiber surface membrane, leads to the twitch contraction of the fiber. Myotonic disorders are inherited diseases whose clinical manifestations include electrophysiological signs such as increased...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(87)90032-0
更新日期:1987-04-01 00:00:00
abstract::The maximum activities of some key enzymes of metabolism were studied in lungs of fed and 48-h-starved rats. The maximum activity of hexokinase in the lung is similar to that of other tissues of the body, but lower than that of phosphorylase and 6-phosphofructokinase. High activities of glucose-6-phosphate dehydrogena...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90023-t
更新日期:1991-05-01 00:00:00
abstract::The analysis of RNA from L5178 Y cells, by electrophoreis in 1.7% polyacrylamide gel, after short time labelling with [methyl-3H] methionine, shows that the three molecular species 47S, 46S and 45S RNA from the ribosomal RNA "45S fraction" are methylated. Comparative studies of the kinetics obtained after labelling fo...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80544-5
更新日期:1975-11-01 00:00:00
abstract::The ability to measure the thermodynamic stability of proteins with precision is important for both academic and applied research. Such measurements rely on mathematical models of the protein denaturation profile, i.e. the relation between a global protein signal, corresponding to the folding states in equilibrium, an...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.11.013
更新日期:2016-02-01 00:00:00
abstract::Direct demonstration of the reversible dissociation of native dimeric methionyl-tRNA synthetase from E. coli has been obtained using small angle neutron scattering and deuterated enzyme. Structural parameters of the fully deuterated dimer are very similar to the hydrogenated one. Analysis of the variations of the inte...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80205-4
更新日期:1985-06-01 00:00:00
abstract::The repressible Saccharomyces cerevisiae acid phosphatase (APase) coded by the PHO5 gene is a cell wall protein that follows the yeast secretory pathway. We had previously described the in vivo fate of a multicopy plasmid-encoded modified protein, lacking 15 out of 17 signal peptide amino acids. This modified protein ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90135-4
更新日期:1990-02-01 00:00:00
abstract::Oxidative stress, characterized by the accumulation of reactive oxygen species (ROS), is known to have numerous detrimental effects on the myocardium such as the induction of apoptotic cell death, hypertrophy, fibrosis, dysfunction, and dilatation. Over the past several years, we have shown that mitochondrial NADP(+)-...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.11.016
更新日期:2014-04-01 00:00:00
abstract::Adipose tissue lipoprotein lipase activity (LPL) falls dramatically during experimental infection of mice by Plasmodium chabaudi. The decrease in this activity accounts for the seric accumulation of triacyl-glyceride-rich lipoproteins (chylomicrons mainly and Very Low Density Lipoproteins (VLDL) partly). This loss of ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80414-8
更新日期:1982-02-01 00:00:00
abstract::Identification of proteoglycans in two human malignant mesothelioma cell lines, one with epithelial differentiation and the other with fibroblast-like phenotype, and the effects of epidermal (EGF), insulin-like (IGF-I) and platelet-derived (PDGF-BB) growth factors on the synthesis of hyaluronan (HA) and proteoglycans ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80131-x
更新日期:1999-07-01 00:00:00
abstract::The present review deals with the place of single chain oligonucleotide ligands (aptamers) in affinity chromatography applied to proteins. Aptamers are not the only affinity ligands available but they represent an emerging and highly promising route that advantageously competes with antibodies in immunopurification pr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2017.10.008
更新日期:2018-02-01 00:00:00
abstract::An alkaline aminopeptidase was isolated from the culture medium of Penicillium roqueforti. The enzyme was purified by ammonium sulfate precipitation, filtration on Bio-Gel P-100, chromatography on D.E.A.E.-cellulose and hydroxylapatite, filtration on Bio-Gel P-150 and electrofusing. The purified preparation was homoge...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80246-0
更新日期:1977-01-01 00:00:00
abstract::Cell-free extracts of fungal mycelia of two aflatoxin non-producing isolates of Aspergillus parasiticus (SRRC 163 and SRRC 2043) were utilized for the study of enzyme activities involved in the latter stages of aflatoxin biosynthesis. The post-microsomal fractions (105,000 x g supernatant) of both SRRC 163 and SRRC 20...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90103-4
更新日期:1988-06-01 00:00:00
abstract::The fatty acid composition of animal products (eggs, milk and meat) is the reflect of both the tissue fatty acid biosynthesis and the fatty acid composition of ingested lipids. This relationship is stronger in monogastrics (pigs, poultry and rabbits) than in ruminants, where dietary fatty acids are hydrogenated in the...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2010.02.027
更新日期:2011-01-01 00:00:00
abstract::The vasoactive hormone bradykinin, its N-and C-terminal fragments and some structural analogues were studied by Circular Dichroism. Conformational features of the peptide can be detected by comparative analysis of the various CD spectra recorded as a function of aqueous pH, solvent and temperature. It is shown that th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(79)80316-8
更新日期:1979-01-01 00:00:00
abstract::Split luciferase complementary assay has been used to investigate the effect of WD domain deletion on Apaf-1 oligomerization. Apaf-1 is an adaptor molecule in formation of apoptosome that activates caspase-9, an activation that is a key event in the mitochondrial cell death pathway. Structural studies suggest that nor...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.10.013
更新日期:2021-01-01 00:00:00
abstract::Interferons-alpha (IFN-alpha) are pleiotropic cytokines belonging to type I IFNs, extensively used in the treatment of patients with some types of cancer and viral disease. IFN-alpha can affect tumor cell functions by multiple mechanisms. In addition, these cytokines can promote the differentiation and activity of hos...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.04.006
更新日期:2007-06-01 00:00:00
abstract::The nuclear metabolism of poly(ADP-ribose) is mainly regulated by poly(ADP-ribose) polymerase-1 (PARP-1) and by poly(ADP-ribose) glycohydrolase (PARG). A PARP-like enzyme, V-PARP, and a PARG isoform are present in the extra-nuclear compartment of mammalian cells, even if poly(ADP-ribose) has never been detected therei...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(02)00017-2
更新日期:2002-12-01 00:00:00
abstract::The Rap family of small GTP-binding proteins is composed by four different members: Rap1A, Rap1B, Rap2A and Rap2B. In this work we report the identification and characterization of a fifth member of this family of small GTPases. This new protein is highly homologous to Rap2A and Rap2B, binds labeled GTP on nitrocellul...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.08.007
更新日期:2006-03-01 00:00:00
abstract::The iron absorption by duodenal enterocytes is a key step of its homeostasis. But the control of this absorption is complex and cannot be fully explicated with present knowledge. In a global transcriptome approach, we identified 60 genes over-expressed in hemin (iron) overload in Caco-2 cells, an in vitro model of duo...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.01.002
更新日期:2008-04-01 00:00:00
abstract::Cotranslational translocation of exportable proteins across the RER membrane prior to their release into the extracellular space has been essentially described by use of canine pancreatic microsomal membranes. Intracisternal segregation of nascent secretory proteins was observed to be irreversible and proteolytic remo...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(88)90194-0
更新日期:1988-09-01 00:00:00
abstract::Epigenetic mechanisms are likely to play a role in many complex diseases, the extent of which we only beginning to understand. COPD and asthma are two respiratory diseases subject to strong environmental influences depending on underlying genetic susceptibility. Epigenetic mechanisms such as DNA methylation, histone m...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.07.017
更新日期:2012-11-01 00:00:00
abstract::The systems involving folate and cobalamin have several features in common: 1) their dietary forms require luminal digestion for absorption; 2) intestinal bacteria in the upper intestine synthesize and utilize both vitamins, creating possible competition for the nutrients; 3) there is one major intestinal brush border...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.11.006
更新日期:2016-07-01 00:00:00