Assembly of chloroplast cytochromes b and c.

Abstract:

:The synthesis of holocytochromes in plastids is a catalyzed process. Several proteins, including plastid CcsA, Ccs1, possibly CcdA and a thioredoxin, plus at least two additional Ccs factors, are required in sub-stoichiometric amounts for the conversion of apocytochromes f and c(6) to their respective holoforms. CcsA, proposed to be a heme delivery factor, and Ccs1, an apoprotein chaperone, are speculated to interact physically in vivo. The formation of holocytochrome b(6) is a multi-step pathway in which at least four, as yet unidentified, Ccb factors are required for association of the b(H) heme. The specific requirement of reduced heme for in vitro synthesis of a cytochrome b(559)-derived holo-beta(2) suggests that cytochrome b synthesis in PSII might also be catalyzed in vivo.

journal_name

Biochimie

journal_title

Biochimie

authors

Nakamoto SS,Hamel P,Merchant S

doi

10.1016/s0300-9084(00)00605-2

keywords:

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

603-14

issue

6-7

eissn

0300-9084

issn

1638-6183

pii

S0300-9084(00)00605-2

journal_volume

82

pub_type

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