Probing the active site of beta-lactamase R-TEM1 by informational suppression.

abstract：:Staphylococcus aureus is one of the most dreaded pathogens worldwide and emergence of notorious antibiotic resistant strains have further exacerbated the present scenario. The glycolytic enzyme, triosephosphate isomerase (TIM) is one of the cell envelope proteins of the coccus and is involved in biofilm formation. It ...

journal_title：Biochimie

authors： Mukherjee S,Roychowdhury A,Dutta D,Das AK

更新日期：2012-12-01 00:00:00

abstract：:Organisms exposed to ionizing radiation (IR) undergo increases in the production of reactive oxygen species (ROS), which are determinant components in the induction of apoptosis. Sensitive to apoptosis gene (SAG) encodes a redox-inducible and apoptosis-protective antioxidant protein. This report demonstrates that the ...

journal_title：Biochimie

authors： Kim SY,Yang ES,Lee YS,Lee J,Park JW

更新日期：2011-02-01 00:00:00

abstract：:The cartilage tissue has a limited self-regenerative capacity. Tissue-engineering represents a promising trend for cartilage repair. The present study was aimed to develop a biomaterial formulation by combining fragments of chitosan hydrogel with isolated rabbit or human chondrocytes. We first reported the properties ...

journal_title：Biochimie

authors： Montembault A,Tahiri K,Korwin-Zmijowska C,Chevalier X,Corvol MT,Domard A

更新日期：2006-05-01 00:00:00

abstract：:The two subunits of the nickel-iron hydrogenase from Desulfovibrio gigas have been purified by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis and their amino acid compositions have been determined. The N-terminal sequences for 15 residues of the large subunit (Mr 62,000) and 25 residues of the s...

journal_title：Biochimie

authors： Nivière V,Forget N,Bovier-Lapierre G,Bonicel J,Hatchikian C

更新日期：1988-02-01 00:00:00

abstract：:Influenza virus-associated RNA polymerase is composed of one molecule each of three viral P proteins and carries the complete activity of capped RNA-primed vRNA-directed transcription. The RNA polymerase holoenzyme also carries capped RNA endonuclease to generate capped oligonucleotide primers for transcription and 3'...

journal_title：Biochimie

authors： Ishihama A

更新日期：1996-01-01 00:00:00

abstract：:A major tolerance mechanism that functions to replicate damaged genomic DNA across lesions that have escaped elimination by repair mechanism is translesion DNA synthesis (TLS). DNA polymerase kappa (Pol kappa), a specialised low-fidelity DNA polymerase which is able to perform DNA synthesis across several damaged base...

journal_title：Biochimie

authors： Bavoux C,Hoffmann JS,Cazaux C

更新日期：2005-07-01 00:00:00

abstract：:Affinity chromatography on cross-linked starch affords a simple and rapid procedure for alpha-amylases (EC 3.2.1.1.) purification. When starch is cross-linked in alkaline medium by epichlorhydrin in the conditions described, the insoluble polysaccharide obtained is able to retain specifically the alpha-amylase which i...

journal_title：Biochimie

authors： Weber M,Foglietti MJ,Percheron F

更新日期：1976-01-01 00:00:00

abstract：:In most cancer cells, the ATP necessary for survival and proliferation is derived from glycolysis rather than from oxidative phosphorylations (OXPHOS) even when oxygen supply would be adequate to sustain them. This phenomenon, named "aerobic glycolysis" by Warburg many years ago, can now be explained by a mechanism up...

journal_title：Biochimie

authors： Hervouet E,Simonnet H,Godinot C

更新日期：2007-09-01 00:00:00

abstract：:Acetylcholinesterase (AChE) has been identified and purified from the venom of desert cobra (W aegyptia) to apparent homogeneity using a TSK G 3000 SW gel filtration column and a Mono Q anion-exchange column. AChE was purified to homogeneity as established by sodium dodecylsulfate/polyacrylamide gel electrophoresis. T...

journal_title：Biochimie

authors： Duhaiman AS,Alhomida AS,Rabbani N,Kamal MA,al-Jafari AA

更新日期：1996-01-01 00:00:00

abstract：:Matrix metalloproteinase 9 (MMP-9) and interleukin-8 (IL-8) play major roles in tumor progression and invasion of breast cancer cells. The present study was undertaken to investigate the inhibitory mechanism of cell invasion by luteolin 8-C-β-fucopyranoside (named as LU8C-FP), a C-glycosylflavone, in human breast canc...

journal_title：Biochimie

authors： Park SH,Kim JH,Lee DH,Kang JW,Song HH,Oh SR,Yoon DY

更新日期：2013-11-01 00:00:00

abstract：:Neprilysin is also known as skin fibroblast-derived elastase, and its up-regulation during aging is associated with impairments of the elastic fiber network, loss of skin elasticity and wrinkle formation. However, information on its elastase activity is still limited. The aim of this study was to investigate the degra...

journal_title：Biochimie

authors： Mora Huertas AC,Schmelzer CEH,Luise C,Sippl W,Pietzsch M,Hoehenwarter W,Heinz A

更新日期：2018-03-01 00:00:00

abstract：:Parkinson's disease (PD), a neurodegenerative disorder, is characterized by a loss of dopaminergic neurons in the substantia nigra (SN) of the brain and it is well known that the pathogenesis of PD is related to a number of risk factors including oxidative stress. Antioxidant 1 (ATOX1) protein plays a crucial role in ...

journal_title：Biochimie

authors： Eum WS,Shin MJ,Lee CH,Yeo HJ,Yeo EJ,Choi YJ,Kwon HJ,Kim DS,Kwon OS,Lee KW,Han KH,Park J,Kim DW,Choi SY

更新日期：2019-01-01 00:00:00

abstract：:In this contribution, we have studied the dynamics of electron transfer (ET) of a flavoprotein to the bound cofactor, an important metabolic process, in a model molecular/macromolecular crowding environments. Vitamin B(2) (riboflavin, Rf) and riboflavin binding protein (RBP) are used as model cofactor and flavoprotein...

journal_title：Biochimie

authors： Rakshit S,Saha R,Verma PK,Mitra RK,Pal SK

更新日期：2012-12-01 00:00:00

abstract：:The hydrolysis of trioctanoin and of monoolein by rat intestinal homogenates, catalyzed by the previously described "trioctanoin hydrolase" and "monoglyceride lipase" [Ref. 6 and 11], was shown to be entirely due to the action of the intestinal glycerol-ester hydrolase [Ref. 1 and 2] and to contaminating pancreatic li...

journal_title：Biochimie

authors： Negrel R,Serrero G,Ailhaud G

更新日期：1977-01-01 00:00:00

abstract：:Ligation of CD47 by its natural ligand thrombospondin (TSP), or cross-linking by CD47 antibodies, triggers caspase-independent cell death in normal and leukemic cells. This kind of cell death is characterised by the cytoplasmic events of apoptosis including externalisation of phosphatidylserines and mitochondria swell...

journal_title：Biochimie

authors： Roué G,Bitton N,Yuste VJ,Montange T,Rubio M,Dessauge F,Delettre C,Merle-Béral H,Sarfati M,Susin SA

更新日期：2003-08-01 00:00:00

abstract：:Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neu1 physiologic...

journal_title：Biochimie

authors： Ryuzono S,Takase R,Kamada Y,Ikenaga T,Chigwechokha PK,Komatsu M,Shiozaki K

更新日期：2017-04-01 00:00:00

abstract：:The protein "BCL-2-associated athanogene-1" (BAG-1), which exists in multiple isoforms, promotes cancer cell survival and is overexpressed in many different cancers. As a result, BAG-1-targeted therapy appears to be a promising strategy with which to treat cancer. It has previously been shown that the 5'UTR of the BAG...

journal_title：Biochimie

authors： Bolduc F,Turcotte MA,Perreault JP

更新日期：2020-09-01 00:00:00

abstract：:In the present study we have investigated the thermal stability of the globular transport protein human serum albumin (HSA), in the presence of two small chain polyethylene glycols (namely PEG 200 and PEG 400). Both near- and far-UV circular dichroism (CD) study reveal that addition of PEG moderately increases the α-h...

journal_title：Biochimie

authors： Samanta N,Mahanta DD,Hazra S,Kumar GS,Mitra RK

更新日期：2014-09-01 00:00:00

abstract：:The single channel data for 4 different linear gramicidins containing either 4 Trp, 4 Phe, 4 Tyr or TyrBzl have been analyzed on the basis of 3 barriers-2 sites model. They form 2 families which differ by their single channel behavior and thus different energy profiles of the channel. A relationship between the surfac...

journal_title：Biochimie

authors： Heitz F,Van Mau N,Bennes R,Daumas P,Trudelle Y

更新日期：1989-01-01 00:00:00

abstract：:Long-term treatment with ambroxol (ABX), a bronchial expectorant, was found to prevent acute exacerbation of chronic obstructive pulmonary disease (AECOPD). The underlying mechanism remains unclear. To address this, we have investigated the effect of ABX on critical antioxidant proteins thioredoxin (Trx) and thioredox...

journal_title：Biochimie

authors： Huang J,Xu J,Tian L,Zhong L

更新日期：2014-02-01 00:00:00

abstract：:In the recent years, we have provided evidence that Tumor Protein 53-Induced Nuclear Protein 1 (TP53INP1) is a key stress protein with antioxidant-associated tumor suppressive function. The TP53INP1 gene, which is highly conserved in mammals, is over-expressed during stress responses including inflammation. This gene ...

journal_title：Biochimie

authors： Saadi H,Seillier M,Carrier A

更新日期：2015-11-01 00:00:00

abstract：:In recent years an increasing number of inherited decreases in man has been identified in which there is an impairment of one or more peroxisomal functions. Sofar 15 different peroxisomal disorders have been identified which can be subdivided into three distinct groups depending upon whether there is a generalized (gr...

journal_title：Biochimie

authors： Wanders RJ,Schutgens RB,Barth PG,Tager JM,van den Bosch H

更新日期：1993-01-01 00:00:00

abstract：:The hbs operon of Bacillus subtilis comprises a single gene which is localized between the spoIVA and mtrA open reading frames, and is situated at 204 degrees of the standard map of B subtilis. Expression of hbs is initiated from two distinct promoters called P1 and P2. The transcription initiation sites have been map...

journal_title：Biochimie

authors： Micka B,Marahiel MA

更新日期：1992-07-01 00:00:00

abstract：:pH-Dependence of hydrolytic activity of trypsin has been studied in cationic reverse micellar system of cetyltrimethylammonium bromide (CTAB) in (50% v/v) chloroform/isooctane using a positively charged substrate N(alpha)-benzoyl-L-arginine ethyl ester (BAEE). The pH of the medium was varied from 4.0 to 8.5 with addit...

journal_title：Biochimie

authors： Fadnavis NW,Babu RL,Deshpande A

更新日期：1998-12-01 00:00:00

abstract：:The ability of asialo-thyroglobulin to bind the thyroid RHL-1 subunit of the asialoglycoprotein receptor has been investigated. Ligand blot assays show that the recombinant carbohydrate recognition domain of the thyroid RHL-1 subunit specifically interacts with rat desialated thyroglobulin. Moreover, RT-PCR and Wester...

journal_title：Biochimie

authors： Pacifico F,Liguoro D,Acquaviva R,Formisano S,Consiglio E

更新日期：1999-05-01 00:00:00

abstract：:Clustered apurinic/apyrimidinic (AP) sites are more cytotoxic than isolated AP lesions because double strand breaks (DSB) can be formed during repair of closely positioned bistranded AP sites. Formation of DSB due to simultaneous cleavage of bistranded AP sites may be regulated by proteins specifically interacting wit...

journal_title：Biochimie

authors： Ilina ES,Khodyreva SN,Lavrik OI

更新日期：2018-07-01 00:00:00

abstract：:Mammalian chymotrypsin-like serine proteases (SPs) are one of the best-studied family of enzymes with roles in a wide range of physiological processes, including digestion, blood coagulation, fibrinolysis and humoral immunity. Extracellular SPs can form cascades, in which one protease activates the zymogen of the next...

journal_title：Biochimie

authors： Veillard F,Troxler L,Reichhart JM

更新日期：2016-03-01 00:00:00

abstract：:NADH cytochrome b5 oxidoreductase (Ncb5or) protects β-cells against oxidative stress and lipotoxicity. The predominant phenotype of lean Ncb5or-null mouse is insulin-dependent diabetes due to β-cell death. This suggests the putative role of NCB5OR polymorphism in human diabetes. Therefore, we aimed to investigate the ...

journal_title：Biochimie

authors： Kálmán FS,Lizák B,Nagy SK,Mészáros T,Zámbó V,Mandl J,Csala M,Kereszturi E

更新日期：2013-07-01 00:00:00

abstract：:Human mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs), the enzymes which esterify tRNAs with the cognate specific amino acid, form mainly a different set of proteins than those involved in the cytosolic translation machinery. Many of the mt-aaRSs are of bacterial-type in regard of sequence and modular structural o...

journal_title：Biochimie

authors： Messmer M,Blais SP,Balg C,Chênevert R,Grenier L,Lagüe P,Sauter C,Sissler M,Giegé R,Lapointe J,Florentz C

更新日期：2009-05-01 00:00:00

abstract：:Leptin is a cytokine secreted by the adipose tissue that is involved in the control of body weight. We previously showed that a point mutation (R105W) in leptin results in leptin deficiency, marked obesity and hypogonadism in humans adults. Expression in COS1 cells showed impaired secretion and intracellular accumulat...

journal_title：Biochimie

authors： Boute N,Zilberfarb V,Camoin L,Bonnafous S,Le Marchand-Brustel Y,Issad T

更新日期：2004-06-01 00:00:00