Abstract:
:The hydrolysis of trioctanoin and of monoolein by rat intestinal homogenates, catalyzed by the previously described "trioctanoin hydrolase" and "monoglyceride lipase" [Ref. 6 and 11], was shown to be entirely due to the action of the intestinal glycerol-ester hydrolase [Ref. 1 and 2] and to contaminating pancreatic lipase firmly bound to the intestinal cells. These conclusions are supported by experiments based on the chromatographic behaviour of the different lipolytic activities, the use of an immunoserum directed against rat pancreatic juice and the relief of bile salt inhibitions by pancreatic colipase. The results are in favour of the existence of a single lipolytic enzyme in the rat intestinal mucosa, i.e. glycerol-ester hydrolase, active against short- and medium chain mono-, di- and triglycerides and against long chain monoglycerides.
journal_name
Biochimiejournal_title
Biochimieauthors
Negrel R,Serrero G,Ailhaud Gdoi
10.1016/s0300-9084(77)80320-9subject
Has Abstractpub_date
1977-01-01 00:00:00pages
433-8issue
4eissn
0300-9084issn
1638-6183pii
S0300-9084(77)80320-9journal_volume
59pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::The single channel data for 4 different linear gramicidins containing either 4 Trp, 4 Phe, 4 Tyr or TyrBzl have been analyzed on the basis of 3 barriers-2 sites model. They form 2 families which differ by their single channel behavior and thus different energy profiles of the channel. A relationship between the surfac...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90136-3
更新日期:1989-01-01 00:00:00
abstract::The effects of DNA oligonucleotides complementary to potato spindle tuber viroid (PSTVd) on viroid infection were investigated. The oligonucleotides were used to form hybrids with PSTVd in the infection mixture. A 75% reduction of viroid infection was found when an oligonucleotide complementary to nucleotides 79-110 o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90026-o
更新日期:1993-01-01 00:00:00
abstract::Protein 3D structures support their biological functions. As the number of protein structures is negligible in regards to the number of available protein sequences, prediction methodologies relying only on protein sequences are essential tools. In this field, protein secondary structure prediction (PSSPs) is a mature ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.09.006
更新日期:2020-12-01 00:00:00
abstract::In vitro cycling rates of E. coli ribosomes and of elongation factors EF-Tu and EF-G have been obtained and these are compatible with translation rates in vivo. We show that the rate of translocation is faster than 50 s-1 and therefore that the EF-G function is not a rate limiting step in protein synthesis. The in viv...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90244-1
更新日期:1988-05-01 00:00:00
abstract::The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.05.010
更新日期:2005-11-01 00:00:00
abstract::An immunological demonstration of alpha-fetoprotein (AFP) in uterine cytosol from immature rats is reported. The identity between serum AFP and the estrogen binding 4.5 S macromolecular complex of uterine cytosol was demonstrated by the use of a specific immunoadsorbent to AFP. Analysis of the sedimentation profile ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(74)80075-1
更新日期:1974-01-01 00:00:00
abstract::Kex2 is a Ca2+-dependent serine protease from S. cerevisiae. Characterization of the substrate specificity of Kex2 is of particular interest because this protease serves as the prototype of a large family of eukaryotic subtilisin-related proprotein-processing proteases that cleave sites consisting of pairs or clusters...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2016.10.003
更新日期:2016-12-01 00:00:00
abstract::We have previously identified a protein with Mr approximately 40,000 (p40) that binds with high specificity and affinity to the 5'-untranslated leaders of mitochondrial mRNAs in yeast. Here we show that this protein is abundant, comprising about 0.4% of total mitochondrial protein. p40 is present in a cytoplasmic (rho...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90182-z
更新日期:1991-12-01 00:00:00
abstract::Histidine-proline-rich glycoprotein (HPRG), or histidine-rich glycoprotein (HRG), is a serum protein that is synthesized in the liver and is actively internalised by different cells, including skeletal muscle. The multidomain arrangement of HPRG comprises two modules at the N-terminus that are homologous to cystatin b...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.09.024
更新日期:2015-11-01 00:00:00
abstract::Long-term exposure to high doses of caffeine affects several aspects of nitrogen metabolism, such as purine, pyrimidine and urea synthesis. However, little is known about the mechanisms of these changes and if they occur at shorter term. We have studied in isolated hepatocytes: 1) the in vitro effect of high doses of ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90014-4
更新日期:1988-10-01 00:00:00
abstract::Recent work has shown that a DBF4 analog in yeast may be a target of nitrogen-containing bisphosphonates. DBF4 is an essential protein kinase required for DNA replication from primary eukaryotes to humans and appears to play a critical role in the S-phase checkpoint. It is also required for cell migration and cell sur...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.11.010
更新日期:2014-04-01 00:00:00
abstract::The periplasmic histidine permease of Salmonella typhimurium is composed of a soluble histidine-binding protein and three membrane-bound components. These latter are produced in very small amounts and only two, the Q and the P protein, have been previously identified. This paper describes the construction of a plasmid...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80242-x
更新日期:1985-01-01 00:00:00
abstract::Lysosomal cysteine proteinases were fractionated from partially purified rat muscle lysosomes. By gel filtration on Sephadex G75, cathepsin D was separated from two thiol-requiring proteolytic fractions of Mr 25 000 and 55 000, respectively. By chromatofocusing, the first fraction (Mr = 25 000) was resolved into three...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(84)90114-7
更新日期:1984-09-01 00:00:00
abstract::For efficient DNA hydrolysis, Type III restriction endonuclease EcoP15I interacts with two inversely oriented recognition sites in an ATP-dependent process. EcoP15I consists of two methylation (Mod) subunits and a single restriction (Res) subunit yielding a multifunctional enzyme complex able to methylate or to hydrol...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.11.014
更新日期:2013-04-01 00:00:00
abstract::Glutathione, being a major intracellular redox regulator has been shown to be implicated in regulation of airway reactivity and inflammation. However, no study so far has investigated the effect of glutathione depletion/repletion during sensitization and challenge phases separately, which could provide an important in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.04.001
更新日期:2014-08-01 00:00:00
abstract::Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.03.023
更新日期:2015-06-01 00:00:00
abstract::At least 6 N-acetylglucosaminyltransferases (GlcNAc-T I, II, III, IV, V and VI) are involved in initiating the synthesis of the various branches found in complex asparagine-linked oligosaccharides (N-glycans), as indicated below: GlcNAc beta 1-6 GlcNAc-T V GlcNAc beta 1-4 GlcNAc-T VI GlcNAc beta 1-2Man alpha 1-6 GlcNA...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90289-1
更新日期:1988-11-01 00:00:00
abstract::Glyoxalate is an effector of oxidative phosphorylation in isolated mitochondria : it slows down State 3 but does not affect State 4 respiration. This report presents the findings of our study on the mechanism of action of glyoxalate ; these findings are listed below. The inhibition of Stage 3 respiration by glyoxalate...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(75)80145-3
更新日期:1975-01-01 00:00:00
abstract::Leptin is a cytokine secreted by the adipose tissue that is involved in the control of body weight. We previously showed that a point mutation (R105W) in leptin results in leptin deficiency, marked obesity and hypogonadism in humans adults. Expression in COS1 cells showed impaired secretion and intracellular accumulat...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.06.005
更新日期:2004-06-01 00:00:00
abstract::Lactic acid bacteria provide a model system for the in vivo study of mechanisms pertaining to the regulation of sugar transport and metabolism by microorganisms. Recent studies with resting and growing cells of the homofermentative Streptococci and Lactobacilli have yielded evidence for hitherto unsuspected regulatory...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(88)90205-2
更新日期:1988-03-01 00:00:00
abstract::In all living cells 3' ends of RNA are posttranscriptionally elongated or shortened by nucleotidyl transferases and ribonucleases. The detailed analysis of the rpsO mRNA of Escherichia coli presented here demonstrates that transcription terminates in vivo at two sites located seven and eight nucleotides downstream fro...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.02.012
更新日期:2005-09-01 00:00:00
abstract::Conformational flexibility and structural fluctuations play an important role in enzyme activity. A great variety of internal motions ranging over different time scales and of different amplitudes are involved in the catalytic cycle. These different types of motions and their functional consequences are considered in ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(98)80054-0
更新日期:1998-01-01 00:00:00
abstract::With the increase of multidrug resistance, novel anti-leukemia agents with diverse mechanisms of action are required to address this challenge. NK-18, the core region of mammalian derived protein NK-lysin, effectively inhibited the viability of both multidrug resistant and sensitive leukemia cell lines. Meanwhile, thi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.02.001
更新日期:2018-04-01 00:00:00
abstract::Affinity chromatography on cross-linked starch affords a simple and rapid procedure for alpha-amylases (EC 3.2.1.1.) purification. When starch is cross-linked in alkaline medium by epichlorhydrin in the conditions described, the insoluble polysaccharide obtained is able to retain specifically the alpha-amylase which i...
journal_title:Biochimie
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::Chronic hypobaric hypoxia induced muscle atrophy results in decreased physical performance at high altitude. Curcumin has been shown to have muscle sparing effects under cachectic conditions. However, the protective effects of curcumin under chronic hypobaric hypoxia have not been studied till now. Therefore, the pres...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.10.012
更新日期:2019-01-01 00:00:00
abstract::The 11 kb las locus, present on the 50 kb plasmid pCIM1, specifies the production of the lantibiotic lactocin S in Lactobacillus sakei L45. The gene cluster is organized into two oppositely orientated operons, lasAMNTUVPJW (lasA-W) and lasXY, the former of which contains the biosynthetic, immunity and transport genes....
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(02)01420-7
更新日期:2002-05-01 00:00:00
abstract::The oxidation of beta-D-glucose with glucose oxidase generally requires oxygen, which, under normal conditions is present at low concentrations in the reaction medium. Experiments show that glucose oxidase is no longer saturated by oxygen at enzyme concentrations greater than 0.4 mg.ml1. This is due to the decrease in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(75)80160-x
更新日期:1975-01-01 00:00:00
abstract::In most cases, anti-protein aptamers are selected by systematic evolution of ligands by exponential enrichment (SELEX) using purified recombinant protein targets. Cell surface proteins, however, are not easy targets for SELEX due to the difficulties associated with their purification. Here, we developed a novel SELEX ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.02.004
更新日期:2006-07-01 00:00:00
abstract::Human α(1)-acid glycoprotein (AGP) is a positive acute phase plasma protein containing two disulfide bridges. Structural studies have shown that under specific conditions AGP undergoes aggregation. In this study, we analysed the nature of AGP's aggregates formed under reducing and non-reducing conditions at pH 5.5 and...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.09.005
更新日期:2013-02-01 00:00:00
abstract::Coumarins are a group of natural compounds widely distributed in plants. Of late, coumarins and their derivatives have grabbed much attention from the pharmacological and pharmaceutical arena due to their broad range of therapeutical qualities. A coumarin derivative 4-methylesculetin (4-ME) has known to possess effect...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.02.014
更新日期:2013-06-01 00:00:00