[Purification of alpha-amylase by affinity chromatography on cross-linked starch].

abstract：:Bacteria of the Lactococcus (formerly lactic Streptococcus) genus are described to emphasize certain less well-known facts of importance in their successful use as dairy starters. Other streptococci (non-rod forms) are briefly characterized to emphasize their usefulness in industrial milk fermentations. ...

journal_title：Biochimie

authors： Sandine WE

更新日期：1988-04-01 00:00:00

abstract：:Nitrogen fixation in A. vinelandii and R. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the ATP/ADP-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. Although we have been successful in preparing in...

journal_title：Biochimie

authors： Veeger C,Laane C,Scherings G,van Zeeland Wolbers L

更新日期：1978-01-01 00:00:00

abstract：:Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and e...

journal_title：Biochimie

authors： Fortova A,Sebestova E,Stepankova V,Koudelakova T,Palkova L,Damborsky J,Chaloupkova R

更新日期：2013-11-01 00:00:00

abstract：:We describe PGtrans, a new and freely available protein sequence databank (2625 sequences, 554198 amino-acids). This data bank is routinely produced by automatic computer translation of the nucleotide sequence library GenBank. The information needed for the translation process (transcriptional orientation, location of...

journal_title：Biochimie

authors： Claverie JM,Sauvaget I,Bougueleret L

更新日期：1985-05-01 00:00:00

abstract：:The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...

journal_title：Biochimie

authors： Giordano G,Rivière C,Azoulay E

更新日期：1977-01-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae strain LB332 bearing a mutation in the ERG20 gene encoding farnesyl diphosphate synthase (FPPS) synthesizes significantly longer dolichols than the wild type strain FL100 (14-31 and 14-19 isoprene units, respectively). The measurement of the short chain prenyl alcohols excreted into ...

journal_title：Biochimie

authors： Plochocka D,Karst F,Swiezewska E,Szkopińska A

更新日期：2000-08-01 00:00:00

abstract：:Coumarins are a group of natural compounds widely distributed in plants. Of late, coumarins and their derivatives have grabbed much attention from the pharmacological and pharmaceutical arena due to their broad range of therapeutical qualities. A coumarin derivative 4-methylesculetin (4-ME) has known to possess effect...

journal_title：Biochimie

authors： Hemshekhar M,Sunitha K,Thushara RM,Sebastin Santhosh M,Shanmuga Sundaram M,Kemparaju K,Girish KS

更新日期：2013-06-01 00:00:00

abstract：:DNA replication in Escherichia coli is controlled at the initiation stage, possibly by regulation of the essential activity of DnaA protein. The cellular membrane has long been hypothesized to be involved in chromosomal replication. Accumulating evidence, both in vitro and in vivo, that supports the importance of memb...

journal_title：Biochimie

authors： Crooke E

更新日期：2001-01-01 00:00:00

abstract：:The mechanism responsible for creating the division site in the right place at the right time in bacteria is unknown. It has been attributed to the formation of proteolipid domains in the cytoplasmic membrane surrounding the nucleoids. We interpret the growing evidence for this hypothesis by invoking hyperstructures, ...

journal_title：Biochimie

authors： Norris V,Fishov I

更新日期：2001-01-01 00:00:00

abstract：:Microalgae can produce large quantities of triacylglycerols (TAGs) and other neutral lipids that are suitable for making biofuels and as feedstocks for green chemistry. However, TAGs accumulate under stress conditions that also stop growth, leading to a trade-off between biomass production and TAG yield. Recently, in ...

journal_title：Biochimie

authors： Prioretti L,Carriere F,Field B,Avilan L,Montané MH,Menand B,Gontero B

更新日期：2020-02-01 00:00:00

abstract：:Versican and decorin, two proteoglycans (PGs) with contradictory roles in the pathophysiology of cancer, comprise important stromal components in many tumor types and play a crucial role in the progression of cancer. In this study, we provide direct evidence for a significant and stage-related accumulation of versican...

journal_title：Biochimie

authors： Skandalis SS,Theocharis AD,Papageorgakopoulou N,Vynios DH,Theocharis DA

更新日期：2006-09-01 00:00:00

abstract：:The Escherichia coli protein synthesis initiation factor IF2 is a member of the large family of G-proteins. Along with translational elongation factors EF-Tu and EF-G and translational release factor RF-3, IF2 belongs to the subgroup of G-proteins that are part of the prokaryotic translational apparatus. The roles of ...

journal_title：Biochimie

authors： Larigauderie G,Laalami S,Nyengaard NR,Grunberg-Manago M,Cenatiempo Y,Mortensen KK,Sperling-Petersen HU

更新日期：2000-12-01 00:00:00

abstract：:Tryptocidine C (TpcC, cyclo[D-Phe1-Pro2-Trp3-D-Trp4-Asn5-Gln6-Trp7-Val8-Orn9-Leu10]) is a broad-spectrum antimicrobial peptide in the tyrothricin complex produced by a soil bacterium, Brevibacillus parabrevis. Electrospray mass spectrometric studies reveal the oligomerisation of TpcC into dimers and higher oligomers, ...

journal_title：Biochimie

authors： Rautenbach M,Kumar V,Vosloo JA,Masoudi Y,van Wyk RJ,Stander MA

更新日期：2020-12-18 00:00:00

abstract：:Clustered apurinic/apyrimidinic (AP) sites are more cytotoxic than isolated AP lesions because double strand breaks (DSB) can be formed during repair of closely positioned bistranded AP sites. Formation of DSB due to simultaneous cleavage of bistranded AP sites may be regulated by proteins specifically interacting wit...

journal_title：Biochimie

authors： Ilina ES,Khodyreva SN,Lavrik OI

更新日期：2018-07-01 00:00:00

abstract：:Organisms exposed to ionizing radiation (IR) undergo increases in the production of reactive oxygen species (ROS), which are determinant components in the induction of apoptosis. Sensitive to apoptosis gene (SAG) encodes a redox-inducible and apoptosis-protective antioxidant protein. This report demonstrates that the ...

journal_title：Biochimie

authors： Kim SY,Yang ES,Lee YS,Lee J,Park JW

更新日期：2011-02-01 00:00:00

abstract：:Esterases and lipases both hydrolyse ester bonds. Whereas the lipases display high activity towards the aggregated state of its substrate, the esterases typically show highest activity towards the soluble state of its substrate. We have compared the amino acid sequence, the 3D-structure as well as the pH-dependent ele...

journal_title：Biochimie

authors： Fojan P,Jonson PH,Petersen MT,Petersen SB

更新日期：2000-11-01 00:00:00

abstract：:The binding properties of Mg2+, Mn2+ and Co2+ to yeast mitochondrial oligomycin sensitive ATPase complex are studied, as reflected by their catalytic effect (hydrolysis of ATP or pNPP, a pseudo substrate) or by a physical parameter (atomic absorption, electron paramagnetic reasonance of Mn2+, enhanced fluorescence of ...

journal_title：Biochimie

authors： Jallon JM,Spyridakis A,Cosson J

更新日期：1977-01-01 00:00:00

abstract：:We have recently reported that α(2)β(1) and α(1)β(1) isozymes of Na(+)/K(+)-ATPase (NKA) are localized in the caveolae whereas only the α(1)β(1) isozyme of NKA is localized in the non-caveolae fraction of pulmonary artery smooth muscle cell membrane. It is well known that different isoforms of NKA are regulated differ...

journal_title：Biochimie

authors： Dey K,Roy S,Ghosh B,Chakraborti S

更新日期：2012-04-01 00:00:00

abstract：:We have analysed the complete sequence of the Escherichia coli K12 isolate MG1655 genome for chromatin-associated protein binding sites, and compared the predicted location of predicted sites with experimental expression data from 'DNA chip' experiments. Of the dozen proteins associated with chromatin in E. coli, only...

journal_title：Biochimie

authors： Ussery D,Larsen TS,Wilkes KT,Friis C,Worning P,Krogh A,Brunak S

更新日期：2001-02-01 00:00:00

abstract：:Elongation factor 1 alpha (EF-1 alpha) was purified to homogeneity from full-grown oocytes of Xenopus laevis. This protein is encoded by a gene previously shown to be expressed in male and female germ cells, and repressed in somatic cells. The purified protein was identified with EF-1 alpha on criteria of molecular ma...

journal_title：Biochimie

authors： Morales J,Mulner-Lorillon O,Denis H,Bellé R

更新日期：1991-09-01 00:00:00

abstract：:The Rap family of small GTP-binding proteins is composed by four different members: Rap1A, Rap1B, Rap2A and Rap2B. In this work we report the identification and characterization of a fifth member of this family of small GTPases. This new protein is highly homologous to Rap2A and Rap2B, binds labeled GTP on nitrocellul...

journal_title：Biochimie

authors： Paganini S,Guidetti GF,Catricalà S,Trionfini P,Panelli S,Balduini C,Torti M

更新日期：2006-03-01 00:00:00

abstract：:All phytopathogenic fungi have two catalase-peroxidase paralogues located either intracellularly (KatG1) or extracellularly (KatG2). Here, for the first time a secreted bifunctional, homodimeric catalase-peroxidase (KatG2 from the rice blast fungus Magnaporthe grisea) has been produced heterologously with almost 100% ...

journal_title：Biochimie

authors： Zámocký M,Droghetti E,Bellei M,Gasselhuber B,Pabst M,Furtmüller PG,Battistuzzi G,Smulevich G,Obinger C

更新日期：2012-03-01 00:00:00

abstract：:A higher level of N-acetylglucosamine incorporation by proteinic and polyprenic endogenous acceptors is observed after infection by Myxovirus. This phenomenon occurs in whole mitochondria as in outer mitochondrial membranes, where it is particularly obvious with proteinic endogenous acceptors. Under viral infection, n...

journal_title：Biochimie

authors： Gateau O,Morelis R,Louisot P

更新日期：1980-01-01 00:00:00

abstract：:The rat and rabbit lung isoform pattern before (fetal) and after (adult) lung maturation has been analyzed by isoelectric focusing. This pattern has been compared to that of brain tubulin at the same developmental stages. No changes in the pattern of fetal and adult lung tubulin were found. However an increase in brai...

journal_title：Biochimie

authors： Déiz JC,Avila J

更新日期：1985-09-01 00:00:00

abstract：:The structure of ATP synthase subunit 4 was determined by using the oligonucleotide probe procedure. This subunit is the fourth polypeptide of the complex when classifying subunits in order of decreasing molecular mass. Its relative molecular mass is 25 kDa. The ATP4 gene was isolated and sequenced. The nucleotide seq...

journal_title：Biochimie

authors： Velours J,Arselin G,Paul MF,Galante M,Durrens P,Aigle M,Guerin B

更新日期：1989-08-01 00:00:00

abstract：:Obesity prevalence continues to rise throughout the developed world, as a result of positive energy balance and reduced physical activity. At present, there is still a perception within the general community, and amongst some nutritionists, that eating multiple small meals spaced throughout the day is beneficial for w...

journal_title：Biochimie

authors： Hutchison AT,Heilbronn LK

更新日期：2016-05-01 00:00:00

abstract：:With the relation between chemical structure and pharmacological activity as a guide, we have been for some time synthetizing a wide range of beta-amino-ketone derivatives. One of them, 2-(4-methyl-1-piperazinylmethyl) acrylophenone, MPMAP, possesses antimicrotubular activities. This product inhibits 50% of the microt...

journal_title：Biochimie

authors： Mallevais ML,Delacourte A,Lesieur I,Lesieur D,Cazin M,Brunet C,Luyckx M

更新日期：1984-06-01 00:00:00

abstract：:Interferons-alpha (IFN-alpha) are pleiotropic cytokines belonging to type I IFNs, extensively used in the treatment of patients with some types of cancer and viral disease. IFN-alpha can affect tumor cell functions by multiple mechanisms. In addition, these cytokines can promote the differentiation and activity of hos...

journal_title：Biochimie

authors： Ferrantini M,Capone I,Belardelli F

更新日期：2007-06-01 00:00:00

abstract：:We have analyzed changes in the supercoiling of nucleoid DNA of murine fibroblasts cultured in the presence of hydralazine. The entire DNA attached to the nuclear matrix was extracted from the cells and sedimented in neutral sucrose density gradients containing ethidium bromide. Nucleoids from cells treated with hydra...

journal_title：Biochimie

authors： Weglarz L,Koceva-Chyła A

更新日期：1998-07-01 00:00:00

abstract：:Murine L5178Y cell ribosomes were dissociated into subunits either with potassium chloride in the presence of puromycin or with the chelating agent EDTA. The proteins of ribosomal subunits obtained by these different methods were compared by means of bidimensional polyacrylamide gel electrophoresis. KCl-derived 60S an...

journal_title：Biochimie

authors： Longuet M,Auger-Buendia MA,Tavitian A

更新日期：1979-01-01 00:00:00