Abstract:
:Tryptocidine C (TpcC, cyclo[D-Phe1-Pro2-Trp3-D-Trp4-Asn5-Gln6-Trp7-Val8-Orn9-Leu10]) is a broad-spectrum antimicrobial peptide in the tyrothricin complex produced by a soil bacterium, Brevibacillus parabrevis. Electrospray mass spectrometric studies reveal the oligomerisation of TpcC into dimers and higher oligomers, analogous to tyrocidine C (TrcC, Trp7 replaced by Tyr7). Ion mobility mass spectrometry (IMMS) further confirms the formation of stable peptide dimers and tetramers with diameters of 2.7 nm and 3.3 nm, respectively, calculated from collisional cross section (CCS). Molecular dynamic simulations and docking studies support the formation of amphipathic dimers, with a diameter of 2.5 ± 0.07 nm calculated from low energy model CCS. Circular dichroism and IMMS studies point towards dynamic hydrogen-bonded conformational changes up to 28-33 μM after which the structures become more static (or in equilibrium). Fluorescence studies indicate aromatic stacking of Trp residues with a CMC of 18 μM in aqueous solutions. The concentration and time dependent interaction of Trp in oligomers indicate cooperativity in the TpcC oligomerisation that leads to the formation of higher order microscopic structures. Scanning electron microscopy studies unequivocally shows that TpcC forms nanospheres with a mean diameter of 25 nm. Repeated smaller oligomeric units, possibly dimers and tetramers, self-assemble to form these nanospheres.
journal_name
Biochimiejournal_title
Biochimieauthors
Rautenbach M,Kumar V,Vosloo JA,Masoudi Y,van Wyk RJ,Stander MAdoi
10.1016/j.biochi.2020.12.006subject
Has Abstractpub_date
2020-12-18 00:00:00pages
123-133eissn
0300-9084issn
1638-6183pii
S0300-9084(20)30326-6journal_volume
181pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Glycosphingolipids and sphingolipids of cellular plasma membranes (PMs) reach luminal intra-lysosomal vesicles (LVs) for degradation mainly by pathways of endocytosis. After a sorting and maturation process (e.g. degradation of sphingomyelin (SM) and secretion of cholesterol), sphingolipids of the LVs are digested by ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2016.05.004
更新日期:2016-11-01 00:00:00
abstract::Developing a logical and rational methodology for obtaining vaccines, especially against the main parasite causing human malaria (P. falciparum), consists of blocking receptor-ligand interactions. Conserved peptides derived from proteins involved in invasion and having high red blood cell binding ability have thus bee...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.07.006
更新日期:2006-02-01 00:00:00
abstract::The fatty acid composition of animal products (eggs, milk and meat) is the reflect of both the tissue fatty acid biosynthesis and the fatty acid composition of ingested lipids. This relationship is stronger in monogastrics (pigs, poultry and rabbits) than in ruminants, where dietary fatty acids are hydrogenated in the...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2010.02.027
更新日期:2011-01-01 00:00:00
abstract::Plant sucrose transporters (SUTs) are functional as sucrose-proton-cotransporters with an optimal transport activity in the acidic pH range. Recently, the pH optimum of the Solanum tuberosum sucrose transporter StSUT1 was experimentally determined to range at an unexpectedly low pH of 3 or even below. Various research...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.07.028
更新日期:2013-11-01 00:00:00
abstract::Although halogenated hydrocarbons are noted for low chemical reactivity, small amounts are toxic to humans. Cytochromes P450 have been implicated in transforming these compounds to more reactive species, under anaerobic conditions, through reduction at the heme. A significant amount of effort has been directed toward ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(97)82529-1
更新日期:1996-01-01 00:00:00
abstract::GcvB is a non-coding RNA that regulates oppA mRNA in different bacterial species by binding a GcvB GU-rich region named R1 to oppA mRNA. A secondary putative interaction site (PS1) was identified in this study that is able to form a second nearly perfect 10 base-pair duplex between these two RNAs in Escherichia coli. ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.06.020
更新日期:2010-10-01 00:00:00
abstract::Since 2012, the interest for TMEM165 increased due to its implication in a rare genetic human disease named TMEM165-CDG (Congenital Disorder(s) of Glycosylation). TMEM165 is a Golgi localized protein, highly conserved through evolution and belonging to the uncharacterized protein family 0016 (UPF0016). Although the pr...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.07.016
更新日期:2019-10-01 00:00:00
abstract::In most cases, anti-protein aptamers are selected by systematic evolution of ligands by exponential enrichment (SELEX) using purified recombinant protein targets. Cell surface proteins, however, are not easy targets for SELEX due to the difficulties associated with their purification. Here, we developed a novel SELEX ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.02.004
更新日期:2006-07-01 00:00:00
abstract::Administration of beta-adrenergic agonists to domestic species can lead to skeletal muscle hypertrophy, probably by reducing the rate of myofibrillar protein breakdown. Myofibrillar breakdown is associated with the calcium-dependent proteinase system (calpains I,II and calpastatin) whose activity also changes during b...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(92)90125-x
更新日期:1992-03-01 00:00:00
abstract::A variety of species of GPDH undergo acylation at two of the four active cystein sites per mole of tetrameric enzyme. This reaction requires tightly bound NAD+, a situation restricted to two of the four NAD sites per tetramer. S leads to N acyl transfer from cysteins to lysine in the diacyl enzyme yields an inactive e...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(81)80177-0
更新日期:1981-02-01 00:00:00
abstract::Parkinson's disease (PD), a neurodegenerative disorder, is characterized by a loss of dopaminergic neurons in the substantia nigra (SN) of the brain and it is well known that the pathogenesis of PD is related to a number of risk factors including oxidative stress. Antioxidant 1 (ATOX1) protein plays a crucial role in ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.10.010
更新日期:2019-01-01 00:00:00
abstract::Glycogen synthase I was purified from bovine polymorphonuclear leucocytes (PMNs) by a procedure involving concanavalin A-Sepharose affinity chromatography. The purified glycogen-bound glycogen synthase I had a specific activity of 9.83 U/mg protein and the glycogen free enzyme 21 U/mg protein. Molecular ratio of the n...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80201-7
更新日期:1985-06-01 00:00:00
abstract::In recent years an increasing number of inherited decreases in man has been identified in which there is an impairment of one or more peroxisomal functions. Sofar 15 different peroxisomal disorders have been identified which can be subdivided into three distinct groups depending upon whether there is a generalized (gr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(93)90087-9
更新日期:1993-01-01 00:00:00
abstract::At the first symptoms of organisation of the silkgland in the embryo, mitoses stop and nuclei start to grow. Through autoradiographic studies, performed after sequenced labeling with [3H] and [14C] thymidine, the durations of the different phases of DNA synthesis cycles (T = 42 to 48 h, S = 22 to 25 h, G = 20 to 23 h)...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(79)80066-8
更新日期:1979-01-01 00:00:00
abstract::The potent cytotoxicity of reactive oxygen species (ROS) can cause various diseases, however, it may also serve as a powerful chemotherapeutic strategy capable of killing cancer cells. Oxalomalate (OMA, α-hydroxy-β-oxalosuccinic acid), a tricarboxylic acid intermediate, is a well-known competitive inhibitor of two cla...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.01.004
更新日期:2019-03-01 00:00:00
abstract::A thrombin-like enzyme, named BjussuSP-I, isolated from Bothrops jararacussu snake venom, is an acidic single-chain glycoprotein with M(r)=61,000, pI approximately 3.8 and 6% sugar. BjussuSP-I shows high proteolytic activity upon synthetic substrates, such as S-2238 and S-2288. It also shows procoagulant and kallikrei...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.10.005
更新日期:2008-03-01 00:00:00
abstract::The toxicity of amyloids is a subject under intense scrutiny. Many studies link this toxicity to the existence of various intermediate structures prior to the fiber formation and/or their specific interaction with membranes. Membranes can also be a catalyst of amyloidogenesis and the composition or the charge of membr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.07.011
更新日期:2013-01-01 00:00:00
abstract::Lysophosphatidic acid (LPA), a naturally occurring bioactive phospholipid, mediates a multitude of (patho)physiological events including activation of mitogen-activated protein kinases (MAPKs). As LPA may induce cellular reponses in human osteosarcoma, the present study aimed at investigating expression of various LPA...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.05.023
更新日期:2012-09-01 00:00:00
abstract::The present review deals with the place of single chain oligonucleotide ligands (aptamers) in affinity chromatography applied to proteins. Aptamers are not the only affinity ligands available but they represent an emerging and highly promising route that advantageously competes with antibodies in immunopurification pr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2017.10.008
更新日期:2018-02-01 00:00:00
abstract::High glucose (HG)-induced podocyte injury contributes to the pathogenesis of diabetic nephropathy, a severe complication of diabetes. Bromodomain-containing protein 4 (BRD4) has emerged as a critical regulator for cell injury. However, whether BRD4 participates in HG-induced podocyte injury remains unclear. In this st...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.07.012
更新日期:2019-10-01 00:00:00
abstract::The cartilage tissue has a limited self-regenerative capacity. Tissue-engineering represents a promising trend for cartilage repair. The present study was aimed to develop a biomaterial formulation by combining fragments of chitosan hydrogel with isolated rabbit or human chondrocytes. We first reported the properties ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.03.002
更新日期:2006-05-01 00:00:00
abstract::The rate of hydrolysis of long chain triglycerides by pure bovine pancreatic lipase has been determined in the presence of variable amounts of bile salts and colipase. Cofactor-free lipase is strongly inhibited by sodium taurodesoxycholate and by mixed bovine bile salts at concentrations higher than the critical micel...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80572-x
更新日期:1975-01-01 00:00:00
abstract:BACKGROUND:Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by promoting ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.08.003
更新日期:2014-11-01 00:00:00
abstract::Dyskeratosis congenita is an inherited syndrome characterised by mucocutaneous features, bone marrow failure, an increased risk of malignancy and other somatic abnormalities. There is a considerable range of clinical severity and in its occult form the disease may present as idiopathic aplastic anaemia. Genes responsi...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.07.017
更新日期:2008-01-01 00:00:00
abstract::The RecA protein of E coli promotes a strand exchange reaction in vitro which appears to be similar to homologous genetic recombination in vivo. A model for the mechanism of strand transfer reaction by RecA protein has been proposed by Howard-Flanders et al based on the assumption that the RecA monomer has two distinc...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90200-k
更新日期:1991-02-01 00:00:00
abstract::The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.05.010
更新日期:2005-11-01 00:00:00
abstract::The reactivity of sonicated phosphatidylcholine-cholesterol liposomes with cholesterol : oxygene oxydoreductase, an enzyme which catalyses the oxidation of the 3 beta hydroxyl group of cholesterol to a ketone group, is compared with that of ternary system phosphatidylcholine-cholesterol-Thesit. Regardless to the phosp...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(79)80205-9
更新日期:1979-01-01 00:00:00
abstract::Kissing loop interactions (KLIs) are a common motif that is critical in retroviral dimerization, viroid replication, mRNA, and riboswitches. In addition, KLIs are currently used in a variety of biotechnology applications, such as in aptamer sensors, RNA scaffolds and to stabilize vaccines for therapeutics. Here we des...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.11.012
更新日期:2019-02-01 00:00:00
abstract::By use of procollagen proline hydroxylas which catalyses the substitution of the hydrogen in the trans position on carbon 4 with an hydroxyl on L-proline residues of procollagen, we have shown that the L-[3H-4] proline product of C.E.N. Saclay contained 29 per cent of the trans and 71 per cent of the cis form. It was ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80433-6
更新日期:1976-01-01 00:00:00
abstract::Five 4-arylcoumarins (1c-g) and twelve 3,4-dihydro-4-arylcoumarins (2a-l) were synthesized and tested for antioxidant activity, antitumor activity, toxicity and structure-activity relationships analysis. 4-Arylcoumarins and 3,4-dihydro-4-arylcoumarins that possess two hydroxyl groups in ortho position, such as 1d, 1f,...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.03.014
更新日期:2014-12-01 00:00:00