2-(4-methyl-1-piperazinylmethyl) acrylophenone dihydrochloride: a new antimicrotubular drug.

abstract：:The solubilizing powers of taurocholate, taurochenodeoxycholate and tauroursodeoxycholate for monoolein and cholesterol, and the size of the bile salt-monoolein-cholesterol micelles have been determined. For the three bile salt species, the micellar size depends on the saturation with monoolein. As a result, for a giv...

journal_title：Biochimie

authors： Montet JC,Lindheimer M,Reynier MO,Crotte C,Bontemps R,Gerolami A

更新日期：1982-04-01 00:00:00

abstract：:Potato virus A (PVA), a potyvirus with a (+)ssRNA genome translated to a large polyprotein, was engineered and used as a gene vector for expression of heterologous proteins in plants. Foreign genes including jellyfish GFP (Aequorea victoria) encoding the green fluorescent protein (GFP, 27 kDa) and the genes of human o...

journal_title：Biochimie

authors： Kelloniemi J,Mäkinen K,Valkonen JP

更新日期：2006-05-01 00:00:00

abstract：:NDPK-A, NDPK-B and NDPK-D are three enzymes which belong to the NDPK group I isoforms and are not only involved in metabolism process but also in transcriptional regulation, DNA cleavage, histidine protein kinase activity and metastasis development. Those enzymes were reported to bind to membranes either in mitochondr...

journal_title：Biochimie

authors： Francois-Moutal L,Marcillat O,Granjon T

更新日期：2014-10-01 00:00:00

abstract：:Replication protein A (RPA) is a heterotrimeric single-stranded DNA binding protein whose role in DNA replication, recombination and repair has been mainly elucidated through in vitro biochemical studies utilizing the mammalian complex. However, the identification of homologs of all three subunits in Saccharomyces cer...

journal_title：Biochimie

authors： Smith J,Zou H,Rothstein R

更新日期：2000-01-01 00:00:00

abstract：:During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...

journal_title：Biochimie

authors： Girard J,Perdereau D,Narkewicz M,Coupé C,Ferré P,Decaux JF,Bossard P

更新日期：1991-01-01 00:00:00

abstract：:The Escherichia coli Fis protein is a dimeric DNA-binding protein whose specific binding sites share a weak consensus sequence. Use of the gel retardation technique indicates that binding of Fis on a linear DNA fragment leads to the formation of a ladder of defined retarded complexes, independently of the presence of ...

journal_title：Biochimie

authors： Bétermier M,Galas DJ,Chandler M

更新日期：1994-01-01 00:00:00

abstract：:The concept of a stem cell subpopulation as understood from normal epithelial tissue or bone marrow function has been extended to our understanding of cancer tissue and is now the target of treatment efforts specifically directed to this subpopulation. In glioblastoma, as well as in other cancers, increased expression...

journal_title：Biochimie

authors： Rappa F,Cappello F,Halatsch ME,Scheuerle A,Kast RE

更新日期：2013-04-01 00:00:00

abstract：:The vasoactive hormone bradykinin, its N-and C-terminal fragments and some structural analogues were studied by Circular Dichroism. Conformational features of the peptide can be detected by comparative analysis of the various CD spectra recorded as a function of aqueous pH, solvent and temperature. It is shown that th...

journal_title：Biochimie

authors： Lintner K,Fermandjian S,Regoli D

更新日期：1979-01-01 00:00:00

abstract：:Herein, we report the purification and biochemical characterization of a novel bi-functional protein proteinase/amylase inhibitor from the dietary leguminous pulse Phaseolus aureus Roxb. (Vigna radiata L.) by means of acetic acid precipitation, salt fractionation, ion-exchange chromatography (DEAE-cellulose) and affin...

journal_title：Biochimie

authors： Haq SK,Atif SM,Khan RH

更新日期：2005-12-01 00:00:00

abstract：:Apolipoprotein E (apoE) has been genetically linked to late-onset Alzheimer's disease. From the three common alleles (epsilon2, epsilon3 and epsilon4), epsilon4 has been suggested to promote amyloid beta (Ass) plaque fibrillation, one hallmark of Alzheimer's disease. It has been demonstrated that altered lipid content...

journal_title：Biochimie

authors： Rapp A,Gmeiner B,Hüttinger M

更新日期：2006-05-01 00:00:00

abstract：:The functions of residues 57-RY-58, G60, L77, 80-GSGR-83, I101, T104, 134-GY-135, N155, V157 and 160-LV-161 in human arsenic (III) methyltransferase (hAS3MT) 5 Å around S-adenosylmethionine (SAM) have not been studied. Herein, sixteen mutants were designed by substituting these residues with Ala. Mutants G60A, G80A, I...

journal_title：Biochimie

authors： Li X,Geng Z,Chang J,Song X,Wang Z

更新日期：2014-12-01 00:00:00

abstract：:Flavodoxins are one domain alpha/beta electron transfer proteins that participate in photosynthetic reactions. All flavodoxins carry a molecule of flavin mononucleotide (FMN), non-covalently bound, that confers redox properties to the protein. There are two structurally distinct flavodoxins, short ones and long flavod...

journal_title：Biochimie

authors： Maldonado S,Lostao A,Irún MP,Férnandez-Recio J,Gustavo Genzor C,Begoña González E,Rubio JA,Luquita A,Daoudi F,Sancho J

更新日期：1998-10-01 00:00:00

abstract：:The NADPH-oxidase complex, which plays beneficial or detrimental role in the inflammatory and degenerative diseases, is a membrane multi-subunit complex tightly regulated in order to produce superoxide anions, precursor of oxygen reactive species (ROS), in cells. The flavocytochrome b(558) (Cytb(558)) is the catalytic...

journal_title：Biochimie

authors： Souabni H,Machillot P,Baciou L

更新日期：2014-12-01 00:00:00

abstract：:The ability of asialo-thyroglobulin to bind the thyroid RHL-1 subunit of the asialoglycoprotein receptor has been investigated. Ligand blot assays show that the recombinant carbohydrate recognition domain of the thyroid RHL-1 subunit specifically interacts with rat desialated thyroglobulin. Moreover, RT-PCR and Wester...

journal_title：Biochimie

authors： Pacifico F,Liguoro D,Acquaviva R,Formisano S,Consiglio E

更新日期：1999-05-01 00:00:00

abstract：:Two forms of baker's yease valyl-tRNA synthetase have been purified to apparent homogeneity by classical methods. It was demonstrated that one of the two forms of the enzyme originates from the other by proteolysis, the respective amounts of each form depending on the physiological state of the yeast. The species main...

journal_title：Biochimie

authors： Kern D,Giegé R,Robre-Saul S,Boulanger Y,Ebel JP

更新日期：1975-01-01 00:00:00

abstract：:Explants from 7, 8, 9, 11, 13-day chick embryonic skin incorporating (3H) Uridine for different periods 1 hr, 3 or 4 hr and a chase with actinomycin) are studied with respect to free (F) or membrane bound (B) cytoplasmic polysomes and to RNA extracted from them. Polysome specific activity decreases at older stages but...

journal_title：Biochimie

authors： Desveaux-Chabrol J

更新日期：1976-01-01 00:00:00

abstract：:Sticholysin II (StII) is a pore-forming toxin of biomedical interest that belongs to the actinoporin protein family. Sticholysins are currently under examination as an active immunomodulating component of a vaccinal platform against tumoral cells and as a key element of a nucleic acids delivery system to cell cytosol....

journal_title：Biochimie

authors： Soto C,Bergado G,Blanco R,Griñán T,Rodríguez H,Ros U,Pazos F,Lanio ME,Hernández AM,Álvarez C

更新日期：2018-05-01 00:00:00

abstract：:Reuber H 35 hepatoma cells were synchronized by transfer in a serum free medium. Growth was re-initiated by addition of serum. Under these conditions DNA synthesis exhibited a maximum after 24 hours. Chromatin non-histone proteins prepared from cells at various phases of the cell cycle were incubated with [gamma-32P] ...

journal_title：Biochimie

authors： Defer N,Tichonicky L,Kruh J,Zoutewelle G,Van Wijk R

更新日期：1979-01-01 00:00:00

abstract：:Colorectal cancer (CRC) is the third most diagnosed cancer in the western world, affecting 1 out of approximately 22 people in their lifetime. Several epidemiological studies suggest a positive association between high plasma cholesterol levels and colorectal cancer. However, the molecular mechanisms by which choleste...

journal_title：Biochimie

authors： Warns J,Marwarha G,Freking N,Ghribi O

更新日期：2018-10-01 00:00:00

abstract：:The interferon (IFN) system is a major effector of the innate immunity that allows time for the subsequent establishment of an adaptive immune response against wide-range pathogens. The effectiveness of IFN to control initial infection requires the cooperation between several pathways induced in the target cells. Rece...

journal_title：Biochimie

authors： Degols G,Eldin P,Mechti N

更新日期：2007-06-01 00:00:00

abstract：:TcrX/Y is one of the twelve two component system (TCS) present in Mycobacterium tuberculosis. We have investigated the TcrX/Y interaction by in silico studies, pull down assay, radioactive phosphotransfer, surface plasmon resonance as well as crosstalk analysis of TcrY with TcrA - a non-cognate response regulator. Seq...

journal_title：Biochimie

authors： Bhattacharya M,Biswas A,Das AK

更新日期：2010-03-01 00:00:00

abstract：:The interactions between lactic acid bacteria and their phages are commercially significant. Current research has focused on the elucidation of the mechanisms and genetics of phage resistance. Phage resistance genes have been linked to plasmid DNA for Streptococcus lactis and Streptococcus cremoris, and preliminary st...

journal_title：Biochimie

authors： Sanders ME

更新日期：1988-03-01 00:00:00

abstract：:Snake venom phospholipases A2 (PLA2) share high sequence identities and a conserved structural scaffold, but show important functional differences. Only a few PLA2s have been purified and characterized from coral snake (Micrurus spp.) venoms, and their role in envenomation remains largely unknown. In this report, we d...

journal_title：Biochimie

authors： Rey-Suárez P,Núñez V,Saldarriaga-Córdoba M,Lomonte B

更新日期：2017-06-01 00:00:00

abstract：:Toyocamycin (TMC), an adenosine analog has been previously reported to inhibit both number and infectivity of retrovirus particles released by chronically infected cells (Bonar et al., 1970; Riman, 1971; Mauchauffé et al., 1979). We have previously shown that loss of infectivity could result from the incorporation of ...

journal_title：Biochimie

authors： Mathieu-Mahul D,Barque JP,Mauchauffe M,Peraudeau L,Larsen CJ

更新日期：1981-01-01 00:00:00

abstract：:The very end of the chromosome is called the telomere and is composed of DNA repeat sequences and associated proteins. Genetic and biochemical analyses of this complex, the telosome, lead to the hypothesis that transcription and DNA replication are submitted to position effects mediated by the telomere proximity. Telo...

journal_title：Biochimie

authors： Pommier JP,Lebeau J,Ducray C,Sabatier L

更新日期：1995-01-01 00:00:00

abstract：:Isovaleryl-CoA dehydrogenase (IVD) catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA and the transfer of electrons to the electron transfer flavoprotein (ETF). Recombinant human IVD purifies with bound CoA-persulfide. A modified purification protocol was developed to isolate IVD without bound CoA-pers...

journal_title：Biochimie

authors： Mohsen AW,Vockley J

更新日期：2015-01-01 00:00:00

abstract：:1) Isonitrile derivatives of synthetic polyamides, polyesters and polyalcohols, of polysaccharides such as cellulose, cross-linked dextran and agarose, linear dextran and of cross-linked and linear polyacrylamide were prepared. 2) Proteins and low-molecular-weight ligands were bound covalently to these materials by fo...

journal_title：Biochimie

authors： Goldstein L

更新日期：1980-01-01 00:00:00

abstract：:Lipidomics is a subspecialty of metabolomics that focuses on water insoluble metabolites that form membrane barriers. Most lipidomic databases catalog lipids from common model organisms, like humans or Escherichia coli. However, model organisms' lipid profiles show surprisingly little overlap with those of specialized...

journal_title：Biochimie

authors： Layre E,Moody DB

更新日期：2013-01-01 00:00:00

abstract：:Changes in the cytosolic Ca(2+) concentration ([Ca(2+)](c)) translate a variety of extracellular signals into widely diverse intracellular effects, ranging from secretion to movement, proliferation and also cell death. As regards the last one, it has long been known that large [Ca(2+)](c) increases lead cells to death...

journal_title：Biochimie

authors： Pinton P,Ferrari D,Rapizzi E,Di Virgilio F,Pozzan T,Rizzuto R

更新日期：2002-02-01 00:00:00

abstract：:A neoglycolipid of structure beta-D-Glcp-S-(CH2)3N(OH)(CH2)4-O-cholest-5-en-3 beta-yl has been prepared in fair overall yield by reduction of the nitrone obtained by condensation of beta-D-Glcp-S-(CH2)3NHOH and OCH-(CH2)3-O-cholest-5-en-3 beta-yl. This synthetic procedure is very flexible, allowing a large range of le...

journal_title：Biochimie

authors： Tronchet JM,Zsély M,Ricca A,Barbalat-Rey F,Geoffroy M

更新日期：1992-01-01 00:00:00