Abstract:
:The imprinted gene Grb10 influences fetal growth, adult behavior and tumor formation. Investigating this gene often involves consecutive passaging of cells in vitro. We analyzed changes in Grb10 DNA methylation and histone modifications in serially passaged adult mouse tail-tip fibroblast cells with bisulfite genomic sequencing method and chromatin immunoprecipitation (ChIP) respectively. There was a significant reduction in global DNA methylation, but no changes in methylation at CpG island 1 (CGI1) of Grb10. There were no changes in methylation at histone H3K4me2, but less methylated histone H3K27me3 was detected, along with more of the acetylated histones H3Ac and H4Ac. The major-type transcript level of Grb10 in fibroblasts increased mildly after extended cultivation. These results suggest consecutive passaging may affect epigenetic modifications of Grb10 in adult fibroblast cells.
journal_name
Biochimiejournal_title
Biochimieauthors
Yao J,Huang Y,Huang R,Shi R,Chen P,Zhu B,Li M,Jiang X,Zheng M,Jiang Y,Yang Xdoi
10.1016/j.biochi.2012.08.009subject
Has Abstractpub_date
2012-12-01 00:00:00pages
2699-705issue
12eissn
0300-9084issn
1638-6183pii
S0300-9084(12)00333-1journal_volume
94pub_type
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