Abstract：
:Structural and motional features in the denatured state of a protein dictate the early folding events starting from that state and these features vary depending upon the nature of the denaturant used. Here, we have attempted to decipher the early events in the folding of Dynein Light Chain protein (DLC8), starting from DMSO-d6 denatured state. Multinuclear NMR experiments were used to obtain the full spectral assignment. The HSQC spectrum shows the presence of two sets of peaks for the residues Met 1, Ser 2, Arg 4, Ala 11, Met 17, Thr 26, Lys 44, Tyr 50, Asn 51, Trp 54, His 55, Val 58, Gly 59, Ser 64, Tyr 65, His 68, Phe 86, Lys 87 indicating the presence of slow conformational transition in the heterogeneous ensemble. Analysis of residual structural propensities with secondary (13)C chemical shifts, (3)J(H(N)(-)H(α)) coupling constants and (1)H-(1)H NOE revealed the presence of local preferences which encompass both native and non-native like structures. The spectral density calculations, as obtained from measured R(1), R(2) and (1)H-(15)N steady state NOE values provide insights into the backbone dynamics on the milli to picosecond timescale. The segment Ser 14 - His 55 exhibits slow motions on the milli- to microsecond timescale arising from conformational exchange. The presence of native like structural preference, as well as conformational exchange classifies the above segment as the nucleation site of folding. Based on the observations, we propose here, the probable hierarchy of folding of DLC8 on dilution of denaturant: the two helices are formed first followed by the formation of β2 and β5.
journal_name
Biochimiejournal_title
Biochimieauthors
Chakraborty S,Mohan PM,Hosur RVdoi
10.1016/j.biochi.2011.10.013subject
Has Abstractpub_date
2012-01-01 00:00:00pages
231-41issue
1eissn
0300-9084issn
1638-6183pii
S0300-9084(11)00407-Xjournal_volume
94pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Vascular endothelial growth factor (VEGF) and αvβ3 integrin are key molecules that actively participate in tumor angiogenesis and metastasis. Some integrin-blocking molecules are currently under clinical trials for cancer and metastasis treatment. However, the mechanism of action of such inhibitors is not completely u...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.04.020
更新日期：2012-08-01 00:00:00
abstract：:Interferons (IFNs) were considered for the treatment of patients with multiple sclerosis (MS) after the demonstration, based on small studies, of the efficacy of type IFN beta in decreasing the frequency of exacerbations in relapsing-remitting multiple sclerosis when administered intrathecally, subcutaneously, or intr...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2007.03.016
更新日期：2007-06-01 00:00:00
abstract：:Plant sucrose transporters (SUTs) are functional as sucrose-proton-cotransporters with an optimal transport activity in the acidic pH range. Recently, the pH optimum of the Solanum tuberosum sucrose transporter StSUT1 was experimentally determined to range at an unexpectedly low pH of 3 or even below. Various research...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.07.028
更新日期：2013-11-01 00:00:00
abstract：:A reliable phylogenetic reconstruction of the evolutionary history of contemporary species depends on a robust identification of the universal common ancestor (UCA) at the root of the Tree of Life (ToL). That root polarizes the tree so that the evolutionary succession of ancestors to descendants is discernable. In eff...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.04.014
更新日期：2017-07-01 00:00:00
abstract：:The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2004.03.004
更新日期：2004-03-01 00:00:00
abstract：:Our principle hypothesis is that the hypothalamic hormone, gonadotropin-releasing hormone (GnRH), is an immunostimulatory hormone and plays a pivotal role in the gender differences in immunity and/or autoimmunity. As a general rule, females display heightened immune responses and heightened endocrinological responsive...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(03)00106-8
更新日期：2003-06-01 00:00:00
abstract：:We have analyzed changes in the supercoiling of nucleoid DNA of murine fibroblasts cultured in the presence of hydralazine. The entire DNA attached to the nuclear matrix was extracted from the cells and sedimented in neutral sucrose density gradients containing ethidium bromide. Nucleoids from cells treated with hydra...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(98)80025-4
更新日期：1998-07-01 00:00:00
abstract：:A non-toxic, direct-acting fibrinolytic serine protease (Bafibrinase) demonstrating thrombolytic and anticoagulant properties was purified from Bacillus sp. strain AS-S20-I. Bafibrinase was monomeric, with a molecular mass of 32.3 kDa. The peptide mass fingerprinting of Bafibrinase revealed only 8.3% sequence coverage...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.02.027
更新日期：2012-06-01 00:00:00
abstract：:Glycogen synthase I was purified from bovine polymorphonuclear leucocytes (PMNs) by a procedure involving concanavalin A-Sepharose affinity chromatography. The purified glycogen-bound glycogen synthase I had a specific activity of 9.83 U/mg protein and the glycogen free enzyme 21 U/mg protein. Molecular ratio of the n...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80201-7
更新日期：1985-06-01 00:00:00
abstract：:Coenzymes are small organic molecules that supply a varied set of reactive groups to protein enzymes, thereby diversifying catalysis beyond the chemistries of amino acid sidechains. As RNA structures begin with a more limited chemical diversity than proteins, it seems likely that RNA enzymes would also use functional ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(02)01404-9
更新日期：2002-09-01 00:00:00
abstract：:Triiodothyronine (T3), injected daily into rats, induces heart hypertrophy. In a recent work we have shown that a single T3 injection stimulates RNA synthesis and modifies the translational products of myocardial mRNAs in reticulocyte lysates, quantitatively and qualitatively. In this work we show that T3 induces smal...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(84)90113-5
更新日期：1984-09-01 00:00:00
abstract：:The product of the spoIIM gene of Bacillus subtilis is required for complete septum migration and forespore engulfment during sporulation. To investigate whether expression of spoIIM is required in the forespore compartment of the sporangium, we have constructed a new integrational vector, pKSV7, which contains temper...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(92)90143-3
更新日期：1992-07-01 00:00:00
abstract：:An efficient phosphotriester methodology based on the use of condensing agents in the presence of several O-nucleophilic catalysts has been developed. ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80169-3
更新日期：1985-07-01 00:00:00
abstract：:Determination of the physicochemical parameters of unilamellar and multilamellar liposomes from concentration data in light of a recent quantitative biodynamic concept which strictly adheres to the CGS unit system (cm, g, s) and the mass/volume chemical unit, mol.cm-3. ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90093-9
更新日期：1987-05-01 00:00:00
abstract：:In 1956, when we started our collaboration, both Alick Isaacs and myself had done previous work on interference between inactive and active influenza viruses. We were aware of the state interference research had reached and of the two alternative explanations that had been envisaged. ...
journal_title：Biochimie
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.biochi.2007.04.007
更新日期：2007-06-01 00:00:00
abstract：:After the assessment of the antitumor activity of the anthracycline pigments, the S peucetius group of metabolites was discovered and eventually doxorubicin, a major anticancer agent of established clinical usefulness was developed in the early seventies. A second generation of compounds followed, represented mainly b...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(98)80003-5
更新日期：1998-03-01 00:00:00
abstract：:Snake venom phospholipases A2 (PLA2) share high sequence identities and a conserved structural scaffold, but show important functional differences. Only a few PLA2s have been purified and characterized from coral snake (Micrurus spp.) venoms, and their role in envenomation remains largely unknown. In this report, we d...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.03.008
更新日期：2017-06-01 00:00:00
abstract：:Binding of indoloquinolines with different aliphatic side chains to a parallel G-quadruplex derived from the MYC promoter sequence was characterized by optical and calorimetric measurements. ITC experiments performed at different temperatures enabled the determination of molar heat capacity changes upon quadruplex bin...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.11.015
更新日期：2019-02-01 00:00:00
abstract：:The function of glycerophosphate and malate-aspartate shuttles during glucose metabolism in two strains of Ehrlich ascites tumor cells was evaluated by several experimental approaches. The activities of the enzymes involved in these shuttle systems were assayed in the cytosolic and mitochondrial compartments after cel...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80055-9
更新日期：1985-02-01 00:00:00
abstract：:Chagasin-type inhibitors comprise natural inhibitors of papain-like cysteine proteases that are distributed among Protist, Bacteria and Archaea. Chagasin was identified in the pathogenic protozoa Trypanosoma cruzi as an approximately 11 kDa protein that is a tight-binding and highly thermostable inhibitor of papain, c...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.11.002
更新日期：2016-03-01 00:00:00
abstract：:The hydrolysis of trioctanoin and of monoolein by rat intestinal homogenates, catalyzed by the previously described "trioctanoin hydrolase" and "monoglyceride lipase" [Ref. 6 and 11], was shown to be entirely due to the action of the intestinal glycerol-ester hydrolase [Ref. 1 and 2] and to contaminating pancreatic li...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80320-9
更新日期：1977-01-01 00:00:00
abstract：:Pleurocidin (GWGSFFKKAAHVGKHVGKAALTHYL-NH(2)), found in skin mucous secretions of the winter flounder Pleuronectes americanus, is known to possess a high potency and broad-spectrum antimicrobial peptide without cytotoxicity. In this study, to investigate the impact of pleurocidin on apoptotic progress, we observed mor...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.07.011
更新日期：2011-10-01 00:00:00
abstract：:The physical maps of cloned recBCD gene regions of Serratia marcescens and Proteus mirabilis were correlated to genes located in this region. The genes thyA, recC, recB, recD and argA were organized as in Escherichia coli. The 3 rec genes code for the 3 different subunits of the RecBCD enzyme and produced enzymes prom...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90104-9
更新日期：1991-04-01 00:00:00
abstract：:Thorium-232 ((232)Th), a natural radionuclide from the actinide family, is abundantly present in monazite and other ores. It is used as one of the prime fuel materials in nuclear industry and may pose an exposure risk to nuclear workers and members of the public. Human erythrocytes, as a classical cellular membrane mo...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.03.008
更新日期：2010-07-01 00:00:00
abstract：:Epigenetic alterations are involved in every step of carcinogenesis. The development of chromatin-modifying agents (CMAs) has provided the ability to fight cancer by reversing these alterations. Currently, four CMAs have been approved for cancer treatment; two DNA demethylating agents and two deacetylase inhibitors. A...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.05.012
更新日期：2012-11-01 00:00:00
abstract：:Strains of Escherichia, Salmonella, Shigella and Yersinia actively enter eukaryotic cells. Several techniques were used to compare and contrast the invasion mechanisms of Salmonella cholerae-suis, Yersinia enterocolitica and Shigella flexneri. Three animal cell lines (CHO, HEp-2 and MDCK) were examined for susceptibil...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90271-4
更新日期：1988-08-01 00:00:00
abstract：:Several inositol isomers and in particular myo-inositol (MI) and D-chiro-inositol (DCI), were shown to possess insulin-mimetic properties and to be efficient in lowering post-prandial blood glucose. In addition, abnormalities in inositol metabolism are associated with insulin resistance and with long term microvascula...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2013.05.011
更新日期：2013-10-01 00:00:00
abstract：:We have previously identified a protein with Mr approximately 40,000 (p40) that binds with high specificity and affinity to the 5'-untranslated leaders of mitochondrial mRNAs in yeast. Here we show that this protein is abundant, comprising about 0.4% of total mitochondrial protein. p40 is present in a cytoplasmic (rho...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90182-z
更新日期：1991-12-01 00:00:00
abstract：:The human liver fatty acid binding protein (hFABP1) participates in cellular long-chain fatty acid trafficking and regulation of lipid metabolism and changes in hFABP1 are associated with an increased risk for type 2 diabetes, cardiovascular disease (CVD), and metabolic syndromes. Gene regulation of hFABP1 is not full...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.08.006
更新日期：2012-02-01 00:00:00
abstract：:In this contribution, we have studied the dynamics of electron transfer (ET) of a flavoprotein to the bound cofactor, an important metabolic process, in a model molecular/macromolecular crowding environments. Vitamin B(2) (riboflavin, Rf) and riboflavin binding protein (RBP) are used as model cofactor and flavoprotein...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.08.005
更新日期：2012-12-01 00:00:00
