Abstract：
:Using a rapid FPLC procedure for the separation of calpain I, calpain II and the inhibitor calpastatin from pork meat, results of proteolytic and inhibitory activity were obtained. Duplicate measurements showed variation coefficients less than 0.13. Compared to others we found lower calpain I activity 1 h post-mortem. Three groups of pigs were randomly selected. One group was exposed to work immediately before slaughter, another group was exposed to work but allowed 30 min rest before slaughter, while the third group was a control group. Samples were taken from m longissimus dorsi 1 h and 6 h post-mortem. Work had no influence on the calpain/calpastatin activity, but a significant decrease in the calpain and calpastatin activity 6 h post-mortem was found. It should be noted that the results are preliminary and are part of an ongoing study. The method described allows separation of three extracts per instrument per day followed by activity determinations of calpains and calpastatin by the casein assay.
journal_name
Biochimiejournal_title
Biochimieauthors
Iversen P,Ertbjerg P,Larsen LM,Monllao S,Møller AJdoi
10.1016/0300-9084(93)90041-psubject
Has Abstractpub_date
1993-01-01 00:00:00pages
869-72issue
10eissn
0300-9084issn
1638-6183pii
0300-9084(93)90041-Pjournal_volume
75pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Secondary structure-forming DNA motifs have achieved infamy because of their association with a variety of human diseases and cancer. The 3rd FASEB summer conference on dynamic DNA structures focused on the mechanisms responsible for the instabilities inherent to repetitive DNA and presented many exciting and novel as...
journal_title：Biochimie
pub_type：
doi：10.1016/j.biochi.2012.10.005
更新日期：2013-02-01 00:00:00
abstract：:In septic shock patients, alterations of plasma phospholipid fatty acid profile have never been described. The purpose of this monocentric, non-interventional, observational prospective study was to describe this fatty acid profile in the early phase of septic shock in intensive care unit. Thirty-seven adult patients ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.08.006
更新日期：2013-11-01 00:00:00
abstract：:The number of available genomic sequences is growing very fast, due to the development of massive sequencing techniques. Sequence identification is needed and contributes to the assessment of gene and species evolutionary relationships. Automated bioinformatics tools are thus necessary to carry out these identificatio...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.08.006
更新日期：2008-04-01 00:00:00
abstract：:The exosome is a complex of eleven subunits in yeast, involved in RNA processing and degradation. Despite the extensive in vivo functional studies of the exosome, little information is yet available on the structure of the complex and on the RNase and RNA binding activities of the individual subunits. The current mode...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.01.011
更新日期：2007-05-01 00:00:00
abstract：:We have previously shown that withdrawal of folic acid led to metabolic reprogramming and a less aggressive phenotype in a mouse cell model of triple-negative breast cancer (TNBC). Herein, we evaluate the effects of folic acid withdrawal on transcriptomic profiles in these cells. Murine cell lines were originally deri...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.04.005
更新日期：2020-06-01 00:00:00
abstract：:While many works analyse lipid composition of cellular nuclei, essentially of liver, few describe bird erythrocyte nuclei. For them very discordant results are reported due to very different methods of preparation. In the method we adopted a good reproducibility is controlled by electron microscopy, evaluation of spec...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80126-5
更新日期：1976-01-01 00:00:00
abstract：:Pleurocidin (GWGSFFKKAAHVGKHVGKAALTHYL-NH(2)), found in skin mucous secretions of the winter flounder Pleuronectes americanus, is known to possess a high potency and broad-spectrum antimicrobial peptide without cytotoxicity. In this study, to investigate the impact of pleurocidin on apoptotic progress, we observed mor...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.07.011
更新日期：2011-10-01 00:00:00
abstract：:The termination of protein synthesis in bacteria requires codon-specific polypeptide release factors RF-1 (UAG/UAA specific) and RF-2 (UGA/UAA specific). We have proposed that release factors mimic tRNA and recognize the stop codon for polypeptide release (Nakamura et al (1996) Cell 87, 147-150). In contrast to the te...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)86715-6
更新日期：1996-01-01 00:00:00
abstract：:alpha-Latrotoxin, a protein toxin present in the venom of black widow spider, interacts with membrane receptors of neurons and other secretory cells to stimulate exocytosis. Two types of receptors have been identified and cloned. Our attention has been focused on the calcium independent receptor, a G-protein coupled r...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(00)01170-6
更新日期：2000-09-01 00:00:00
abstract：:Vegetable oils, consisting principally of triacylglycerols (TAG), are major sources of calories and essential fatty acids in the human diet. The fatty acid composition of TAG is a primary determinant of the nutritional quality and health-promoting properties of vegetable oils. TAG fatty acid composition also affects t...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2020.09.020
更新日期：2020-11-01 00:00:00
abstract：:Toyocamycin (TMC), an adenosine analog has been previously reported to inhibit both number and infectivity of retrovirus particles released by chronically infected cells (Bonar et al., 1970; Riman, 1971; Mauchauffé et al., 1979). We have previously shown that loss of infectivity could result from the incorporation of ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(81)80013-2
更新日期：1981-01-01 00:00:00
abstract：:The human ribosomal protein SA, known also as a precursor of the cell-surface laminin receptor, LAMR, is a protein of the 40S ribosomal subunit. It is homologous to eubacterial ribosomal protein S2p, but has a eukaryote-specific C-terminal domain (CTD) that is responsible in LAMR for the binding of laminin as well as ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.12.005
更新日期：2011-03-01 00:00:00
abstract：:Chagasin-type inhibitors comprise natural inhibitors of papain-like cysteine proteases that are distributed among Protist, Bacteria and Archaea. Chagasin was identified in the pathogenic protozoa Trypanosoma cruzi as an approximately 11 kDa protein that is a tight-binding and highly thermostable inhibitor of papain, c...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.11.002
更新日期：2016-03-01 00:00:00
abstract：:The emergence of drug-resistant Mycobacterium tuberculosis (Mtb) stains has escalated the need for developing more efficient drugs and therapeutic strategies against tuberculosis. Here we functionally annotate a secretory mycobacterial asparaginase Rv1538c (MtA) and describe its biochemical properties. MtA primarily e...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.12.023
更新日期：2021-01-05 00:00:00
abstract：:The open reading frame PA3859 of Pseudomonas aeruginosa encodes an intracellular carboxylesterase belonging to a group of microbial enzymes (EC 3.1.1.1) that catalyze the hydrolysis of aliphatic and aromatic esters with a broad substrate specificity. With few exceptions, for this class of enzymes, belonging to the α/β...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.09.001
更新日期：2010-12-01 00:00:00
abstract：:The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2010.05.009
更新日期：2010-11-01 00:00:00
abstract：:The variable manifestation of phenotypes that occur in patients with neurofibromatosis type 1 (NF1) includes benign and malignant neurocutaneous tumors for which no adequate treatment exists. Cell-based screening of known bioactive compounds library identified the protein phosphatase 2A (PP2A) inhibitor Cantharidin an...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.01.001
更新日期：2017-04-01 00:00:00
abstract：:In this review, we show how the stability of the asymmetric transverse distribution of phospholipids and the physiological role of the asymmetric distribution can be explained. Experiments with paramagnetic or fluorescent lipids enabled us to show that in fresh red blood cells, i.e. containing ATP, and in resealed gho...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(86)80005-0
更新日期：1986-03-01 00:00:00
abstract：:The antiviral activity of L-nucleoside analogs depends in part on the enantioselectivity of nucleoside kinases which catalyse their monophosphorylation. The substrate properties of human recombinant deoxycytidine kinase (dCK) and human recombinant deoxyguanosine kinase (dGK) with respect to L-adenosine and L-guanosine...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(99)00331-4
更新日期：1999-11-01 00:00:00
abstract：:A reliable phylogenetic reconstruction of the evolutionary history of contemporary species depends on a robust identification of the universal common ancestor (UCA) at the root of the Tree of Life (ToL). That root polarizes the tree so that the evolutionary succession of ancestors to descendants is discernable. In eff...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.04.014
更新日期：2017-07-01 00:00:00
abstract：:Hypertension is the leading cause of preventable mortality worldwide, contributing to over 9 million deaths per annum, predominantly owing to cardiovascular disease. The association of obesity, physical inactivity and alcohol with elevated blood pressure (BP) is firmly established. Weight loss or other dietary strateg...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2020.03.016
更新日期：2020-06-01 00:00:00
abstract：:During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90077-e
更新日期：1991-01-01 00:00:00
abstract：:Thyroglobulin is a large-size iodoglycoprotein specific to thyroid tissue and is the substrate for the synthesis of thyroid hormones, thyroxine and 3,5,3'-triiodothyronine. Recent studies, which greatly benefited from recombinant DNA methodologies, improved the knowledge of several structural features of this dimeric ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(89)90057-6
更新日期：1989-02-01 00:00:00
abstract：:Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and e...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.07.025
更新日期：2013-11-01 00:00:00
abstract：:Although up to 25% of glucose released into circulation in the postabsorptive state comes from renal gluconeogenesis, the regulatory mechanisms of this process are still poorly recognized, comparing to hepatic ones. The aim of the present study was to examine if hypoxia-inducible factor-1 (HIF-1) might be involved in ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.02.002
更新日期：2020-01-01 00:00:00
abstract：:We have recently reported that α(2)β(1) and α(1)β(1) isozymes of Na(+)/K(+)-ATPase (NKA) are localized in the caveolae whereas only the α(1)β(1) isozyme of NKA is localized in the non-caveolae fraction of pulmonary artery smooth muscle cell membrane. It is well known that different isoforms of NKA are regulated differ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.12.020
更新日期：2012-04-01 00:00:00
abstract：:During clinical trials, a number of fully characterized molecules are dropped along the way because they do not provide enough benefit for the patient. Some of them show limited side effects and might be of great use for other applications. AS1411 is a nucleolin-targeting aptamer that underwent phase II clinical trial...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.09.009
更新日期：2015-11-01 00:00:00
abstract：:Ceramidase plays an important role in regulating the metabolism of sphingolipids, such as ceramide, sphingosine (SPH), and sphingosine-1-phosphate (S1P), by controlling the hydrolysis of ceramide. Here we report the cloning and biochemical characterization of a neutral ceramidase from the red flour beetle Tribolium ca...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.03.009
更新日期：2011-07-01 00:00:00
abstract：:Since the initial identification of leptin as the product of the ob gene in 1994, the signaling pathways by which this hormone alters cell physiology have been the subject of extensive investigations. The fact that leptin can induce nitric oxide (NO) production was first demonstrated in studies of the pituitary gland ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.06.017
更新日期：2012-10-01 00:00:00
abstract：:The multisubunit Mediator is an evolutionary conserved transcriptional coregulatory complex in eukaryotes. It is needed for the transcriptional regulation of gene expression in general as well as in a gene specific manner. Mediator complex subunits interact with different transcription factors as well as components of...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2017.10.012
更新日期：2018-01-01 00:00:00
